Click here to switch to the map view.

The map label for this gene is cysL [H]

Identifier: 119356275

GI number: 119356275

Start: 483873

End: 484418

Strand: Reverse

Name: cysL [H]

Synonym: Cpha266_0437

Alternate gene names: 119356275

Gene position: 484418-483873 (Counterclockwise)

Preceding gene: 119356276

Following gene: 119356274

Centisome position: 15.46

GC content: 45.79

Gene sequence:

>546_bases
GTGTTTGACTATCGCCTGAAAGTTTTTGACACGGTTGCCCGTCGCCTGAGCTTTACAAAAGCTGCCGAAGAACTATGCAT
TACACAGCCTGCCGTCACAAAGCATATCAAGGAACTTGAAAAACAGTTCAGGCTTCGGCTGTTTGAGCGCGCCGGCAATA
GAATAGCACTGACCCAGGGCGGACTGATGGTTCTCAAACACACATCAATACTGCGCGATATTTCAAAACAACTGGAGTAC
GAAATCAGTATGCTTCATGAATCCCGCAGAGGAACACTTAACCTTGGCGCAAGCACAACGATCGCCCAATATGTACTTCC
GCCTCTTCTTGCTCGCTTTCACGGGATATACCAGGATGTTCAGCTCCATATGCTCAACACCAACACAGAGCAGATTGAAC
AGGCTCTATTGCGAAAGGAAATCGAACCGGGCATTATCGAAGGAGACTCAAAACGCAGGGAAATCAACTACACACCCTTT
GCCCGCGATGAAATTGTTCTGATAGGCAGAAAAAAGAAAAAATATCTCTCTATATCCTTTAAATAA

Upstream 100 bases:

>100_bases
GAGCAGGCATGATTTCGAAACCCTCAAGGTCGGCGACGAGGTTCTTGTCGCCTCAAAAGCATTCAATCCCCTTGTCATCA
AATTGTAAACCATACTGCCA

Downstream 100 bases:

>100_bases
TATGAGTTGGGAAATCTCACTCTTCTTATAAAAATCAGGGCTCCAACAGTAACTCCGCAAAGCGATCTGATTCGGATCGA
TAAAAAAACATTATGTGCAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MFDYRLKVFDTVARRLSFTKAAEELCITQPAVTKHIKELEKQFRLRLFERAGNRIALTQGGLMVLKHTSILRDISKQLEY
EISMLHESRRGTLNLGASTTIAQYVLPPLLARFHGIYQDVQLHMLNTNTEQIEQALLRKEIEPGIIEGDSKRREINYTPF
ARDEIVLIGRKKKKYLSISFK

Sequences:

>Translated_181_residues
MFDYRLKVFDTVARRLSFTKAAEELCITQPAVTKHIKELEKQFRLRLFERAGNRIALTQGGLMVLKHTSILRDISKQLEY
EISMLHESRRGTLNLGASTTIAQYVLPPLLARFHGIYQDVQLHMLNTNTEQIEQALLRKEIEPGIIEGDSKRREINYTPF
ARDEIVLIGRKKKKYLSISFK
>Mature_181_residues
MFDYRLKVFDTVARRLSFTKAAEELCITQPAVTKHIKELEKQFRLRLFERAGNRIALTQGGLMVLKHTSILRDISKQLEY
EISMLHESRRGTLNLGASTTIAQYVLPPLLARFHGIYQDVQLHMLNTNTEQIEQALLRKEIEPGIIEGDSKRREINYTPF
ARDEIVLIGRKKKKYLSISFK

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=169, Percent_Identity=28.9940828402367, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1788481, Length=165, Percent_Identity=29.6969696969697, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI157672245, Length=171, Percent_Identity=29.2397660818713, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI87082132, Length=165, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1788748, Length=161, Percent_Identity=27.3291925465839, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1789173, Length=130, Percent_Identity=34.6153846153846, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI48994962, Length=118, Percent_Identity=34.7457627118644, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=122, Percent_Identity=27.0491803278689, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1789506, Length=171, Percent_Identity=26.9005847953216, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790399, Length=128, Percent_Identity=25, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 21018; Mature: 21018

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDYRLKVFDTVARRLSFTKAAEELCITQPAVTKHIKELEKQFRLRLFERAGNRIALTQG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
GLMVLKHTSILRDISKQLEYEISMLHESRRGTLNLGASTTIAQYVLPPLLARFHGIYQDV
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
QLHMLNTNTEQIEQALLRKEIEPGIIEGDSKRREINYTPFARDEIVLIGRKKKKYLSISF
EEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEC
K
C
>Mature Secondary Structure
MFDYRLKVFDTVARRLSFTKAAEELCITQPAVTKHIKELEKQFRLRLFERAGNRIALTQG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
GLMVLKHTSILRDISKQLEYEISMLHESRRGTLNLGASTTIAQYVLPPLLARFHGIYQDV
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
QLHMLNTNTEQIEQALLRKEIEPGIIEGDSKRREINYTPFARDEIVLIGRKKKKYLSISF
EEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]