Click here to switch to the map view.

The map label for this gene is rho [H]

Identifier: 119356232

GI number: 119356232

Start: 434054

End: 435343

Strand: Direct

Name: rho [H]

Synonym: Cpha266_0393

Alternate gene names: 119356232

Gene position: 434054-435343 (Clockwise)

Preceding gene: 119356231

Following gene: 119356233

Centisome position: 13.85

GC content: 45.27

Gene sequence:

>1290_bases
ATGTCGAACAATTCGTTAGCAAAAGGTCTGGACATCAATGTGCTCCAGAAAAAAAAGGTTTTTGAGCTGCACGCTCTGGC
AAAAGAGATTGGTGTGACTGCAGCCGGTTTACGCAAGGAAGAGCTTATTTTTAAAATCATAGAGGCGCAGTCGCGAAACA
ATGCCGACTCTGAAAGCGGCAACGTCATGATCAATACCGGTGTTCTTCAGGTTATTCCTGAGGGATACGGATTTTTACGC
TCGGCAAATTATAACTATCTCTCTTCACCAGACGATATCTATGTCTCTCCTTCGCAGATCAAGCGGTTCAATATGCGAAC
AGGTGATACGGTTTCAGGTCAGGTTCGAGCTCCAAAGGAGGGCGAGCGTTTTTTTGCTCTGCTTAAAATCAACACCATTG
ATGGTAATGATCCTGAAATCACCAGAGAGCGACCATATTTCGAGAATCTGACGCCGCTGTTTCCTCATGACAGGATTAAG
CTGGAGATCAAGCAATCGGAATATTGCGGCAGGATCATGGATATTTTTACCCCGATAGGTAAAGGTCAGCGAGGCCTTAT
AGTCGCACAGCCGAAAACCGGTAAAACTATGTTGCTTCAGATGATAGCTAACGCGATCATTCAAAATCATCCCGAAATCT
ATCTTATCGTCCTGCTTATCGATGAACGTCCTGAAGAGGTGACGGACATGGCTCGAAGCGTTGAGGCGGAGGTTGTCAGT
TCAACTTTCGATGAAGATCCTGAACGTCATGTTCAGGTTGCCGATATGGTGCTTGAAAAGGCTAAAAGACTTGTTGAGGT
AGGCAGGGATGTTGTTATTCTGCTTGACTCCATAACAAGGCTTGCCAGAGCTCACAATACCATCATTCCACATTCAGGTA
AAATCCTTTCAGGCGGTATTGATGCCAATGCGCTAACAAAGCCGAAACGATTTTTTGGCGCAGCCCGCAATATTGAGGAG
GGTGGAAGCCTCACAATTATTGCCACAGCACTTGTCGATACCGGCTCACGTATGGACGATGTTATTTTTGAGGAGTTCAA
GGGTACTGGCAACATGGAGCTTTTACTCGATCGCAGGCTTTCTGAACGACGAATTTTTCCTGCAATTGATATTCTTCGTT
CAGGAACCCGTAAAGAGGAACTTCTGTTCAGCCGGGAAGAGCTTTCAAGAACCTGGTTGCTCCGGAAATATCTTGCCGAT
AAAAACCCGATCGAGTGTATGGAGTTCATGCGGGAGAAAATGGCTGATACCAAGGATAACAAAGAGTTTTTCAAATATAT
GAATGCCTGA

Upstream 100 bases:

>100_bases
TTGCTTTGGTCATGAGAGCATCATCTTTTCGGGATTTCCTCCCGTCTGATTTTTTTGATACTAATGTCATAATCACGTTT
TGAATACAATTACAAGAACA

Downstream 100 bases:

>100_bases
TATCCCTTTTTGTGCCCTCACGTCTTCTGCACGGCATGGGGATCCGGCGTTGCGTCCGGTTCTCCTTGCGACTTGTTATG
ACGGCACTCCGGTCTTCTCT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSNNSLAKGLDINVLQKKKVFELHALAKEIGVTAAGLRKEELIFKIIEAQSRNNADSESGNVMINTGVLQVIPEGYGFLR
SANYNYLSSPDDIYVSPSQIKRFNMRTGDTVSGQVRAPKEGERFFALLKINTIDGNDPEITRERPYFENLTPLFPHDRIK
LEIKQSEYCGRIMDIFTPIGKGQRGLIVAQPKTGKTMLLQMIANAIIQNHPEIYLIVLLIDERPEEVTDMARSVEAEVVS
STFDEDPERHVQVADMVLEKAKRLVEVGRDVVILLDSITRLARAHNTIIPHSGKILSGGIDANALTKPKRFFGAARNIEE
GGSLTIIATALVDTGSRMDDVIFEEFKGTGNMELLLDRRLSERRIFPAIDILRSGTRKEELLFSREELSRTWLLRKYLAD
KNPIECMEFMREKMADTKDNKEFFKYMNA

Sequences:

>Translated_429_residues
MSNNSLAKGLDINVLQKKKVFELHALAKEIGVTAAGLRKEELIFKIIEAQSRNNADSESGNVMINTGVLQVIPEGYGFLR
SANYNYLSSPDDIYVSPSQIKRFNMRTGDTVSGQVRAPKEGERFFALLKINTIDGNDPEITRERPYFENLTPLFPHDRIK
LEIKQSEYCGRIMDIFTPIGKGQRGLIVAQPKTGKTMLLQMIANAIIQNHPEIYLIVLLIDERPEEVTDMARSVEAEVVS
STFDEDPERHVQVADMVLEKAKRLVEVGRDVVILLDSITRLARAHNTIIPHSGKILSGGIDANALTKPKRFFGAARNIEE
GGSLTIIATALVDTGSRMDDVIFEEFKGTGNMELLLDRRLSERRIFPAIDILRSGTRKEELLFSREELSRTWLLRKYLAD
KNPIECMEFMREKMADTKDNKEFFKYMNA
>Mature_428_residues
SNNSLAKGLDINVLQKKKVFELHALAKEIGVTAAGLRKEELIFKIIEAQSRNNADSESGNVMINTGVLQVIPEGYGFLRS
ANYNYLSSPDDIYVSPSQIKRFNMRTGDTVSGQVRAPKEGERFFALLKINTIDGNDPEITRERPYFENLTPLFPHDRIKL
EIKQSEYCGRIMDIFTPIGKGQRGLIVAQPKTGKTMLLQMIANAIIQNHPEIYLIVLLIDERPEEVTDMARSVEAEVVSS
TFDEDPERHVQVADMVLEKAKRLVEVGRDVVILLDSITRLARAHNTIIPHSGKILSGGIDANALTKPKRFFGAARNIEEG
GSLTIIATALVDTGSRMDDVIFEEFKGTGNMELLLDRRLSERRIFPAIDILRSGTRKEELLFSREELSRTWLLRKYLADK
NPIECMEFMREKMADTKDNKEFFKYMNA

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=422, Percent_Identity=58.7677725118483, Blast_Score=508, Evalue=1e-145,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 48512; Mature: 48380

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNSLAKGLDINVLQKKKVFELHALAKEIGVTAAGLRKEELIFKIIEAQSRNNADSESG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCCCC
NVMINTGVLQVIPEGYGFLRSANYNYLSSPDDIYVSPSQIKRFNMRTGDTVSGQVRAPKE
CEEEECCCEEECCCCCCHHCCCCCCCCCCCCCEEECHHHHHHCCCCCCCCCCCCEECCCC
GERFFALLKINTIDGNDPEITRERPYFENLTPLFPHDRIKLEIKQSEYCGRIMDIFTPIG
CCEEEEEEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCC
KGQRGLIVAQPKTGKTMLLQMIANAIIQNHPEIYLIVLLIDERPEEVTDMARSVEAEVVS
CCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
STFDEDPERHVQVADMVLEKAKRLVEVGRDVVILLDSITRLARAHNTIIPHSGKILSGGI
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCEEECCC
DANALTKPKRFFGAARNIEEGGSLTIIATALVDTGSRMDDVIFEEFKGTGNMELLLDRRL
CCCCCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEHHHH
SERRIFPAIDILRSGTRKEELLFSREELSRTWLLRKYLADKNPIECMEFMREKMADTKDN
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
KEFFKYMNA
HHHHHHHCC
>Mature Secondary Structure 
SNNSLAKGLDINVLQKKKVFELHALAKEIGVTAAGLRKEELIFKIIEAQSRNNADSESG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCCCC
NVMINTGVLQVIPEGYGFLRSANYNYLSSPDDIYVSPSQIKRFNMRTGDTVSGQVRAPKE
CEEEECCCEEECCCCCCHHCCCCCCCCCCCCCEEECHHHHHHCCCCCCCCCCCCEECCCC
GERFFALLKINTIDGNDPEITRERPYFENLTPLFPHDRIKLEIKQSEYCGRIMDIFTPIG
CCEEEEEEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCC
KGQRGLIVAQPKTGKTMLLQMIANAIIQNHPEIYLIVLLIDERPEEVTDMARSVEAEVVS
CCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
STFDEDPERHVQVADMVLEKAKRLVEVGRDVVILLDSITRLARAHNTIIPHSGKILSGGI
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCEEECCC
DANALTKPKRFFGAARNIEEGGSLTIIATALVDTGSRMDDVIFEEFKGTGNMELLLDRRL
CCCCCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEHHHH
SERRIFPAIDILRSGTRKEELLFSREELSRTWLLRKYLADKNPIECMEFMREKMADTKDN
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
KEFFKYMNA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8051015 [H]