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The map label for this gene is truB [H]

Identifier: 119356210

GI number: 119356210

Start: 415342

End: 416100

Strand: Direct

Name: truB [H]

Synonym: Cpha266_0371

Alternate gene names: 119356210

Gene position: 415342-416100 (Clockwise)

Preceding gene: 119356209

Following gene: 119356211

Centisome position: 13.25

GC content: 49.14

Gene sequence:

>759_bases
ATGGATCAGGAAGGCTTGAAAAGCGTTGCAGGAACTTTGCACACTCCTGAAGTTCATGAGGATTCAGGGTGTTTCCATCT
GGTGGACAAGCCTGTTGACTGGACATCTTTTGACGTGGTTGCCAAGATCCGCAACACATACAAGTTGATCGGACAGAAGC
GAAAGGTCGGCCATTGCGGTACGCTTGATCCGAAAGCAAGCGGGTTGCTCATTCTGGCGACCGGTCGGAAGACCAAAGAG
ATCGCATCTCTTGAACTTCTCGATAAAGTCTATGAGGGATCGTTCAAGCTTGGCGTTGTGACCGACAGTCATGACAGGGA
AACTCCTGAATATAATCAGTGCGCTACAGGGCATTTAGAAAGAGAGCAGATCATTGAAGCGGCTGCTTCTTTTGTTGGCA
CCCGTCAACAGCAGCCTCCGATGTACTCGGCTGCATGGCATAATGGCAAGCGGCTTTATGAACTCGCCCGCAAGGGAGAT
GTTGTTCATGACCGCAAGGCAAAAGAGATTATTATCCGGAGGTTCGAAATTACAGCGGTAGAGCTTCCGCTGGTCCGTTT
TATTCTTGAAGTATCAAAGGGAGCCTATATCCGGGTTATTGCTCATGAGCTTGGTGCTTTGCTTGGAGTGGGCGCCTATC
TTGCATCGCTGAAAAGGGTTGCAATTGGCCCCTATGAGCTCGGTTCGGCATGCAGTGTTCCTGAGACCATAGAAGCTATT
CTCAAAAAGCGCGAGTCGGTATTGTTCATAGAGAAATAA

Upstream 100 bases:

>100_bases
TTCCCGAGCTTGAGTTTTATCAGGATCGTTTATATGAAAAGGCCGACAGAATAGAGCAGTTGCTAAAAGAGGTCAGAAAA
GAACAGGAGTAATCTTGGAG

Downstream 100 bases:

>100_bases
AAAGGCAGTATGCTTGTTGTTGAATATCAGAATAATGACGTTGTTGCATATCCTTCAGGCGAGCCCGTTATTTTTTCTCC
GGTTCCTTCGGCCGTGACGA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MDQEGLKSVAGTLHTPEVHEDSGCFHLVDKPVDWTSFDVVAKIRNTYKLIGQKRKVGHCGTLDPKASGLLILATGRKTKE
IASLELLDKVYEGSFKLGVVTDSHDRETPEYNQCATGHLEREQIIEAAASFVGTRQQQPPMYSAAWHNGKRLYELARKGD
VVHDRKAKEIIIRRFEITAVELPLVRFILEVSKGAYIRVIAHELGALLGVGAYLASLKRVAIGPYELGSACSVPETIEAI
LKKRESVLFIEK

Sequences:

>Translated_252_residues
MDQEGLKSVAGTLHTPEVHEDSGCFHLVDKPVDWTSFDVVAKIRNTYKLIGQKRKVGHCGTLDPKASGLLILATGRKTKE
IASLELLDKVYEGSFKLGVVTDSHDRETPEYNQCATGHLEREQIIEAAASFVGTRQQQPPMYSAAWHNGKRLYELARKGD
VVHDRKAKEIIIRRFEITAVELPLVRFILEVSKGAYIRVIAHELGALLGVGAYLASLKRVAIGPYELGSACSVPETIEAI
LKKRESVLFIEK
>Mature_252_residues
MDQEGLKSVAGTLHTPEVHEDSGCFHLVDKPVDWTSFDVVAKIRNTYKLIGQKRKVGHCGTLDPKASGLLILATGRKTKE
IASLELLDKVYEGSFKLGVVTDSHDRETPEYNQCATGHLEREQIIEAAASFVGTRQQQPPMYSAAWHNGKRLYELARKGD
VVHDRKAKEIIIRRFEITAVELPLVRFILEVSKGAYIRVIAHELGALLGVGAYLASLKRVAIGPYELGSACSVPETIEAI
LKKRESVLFIEK

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=176, Percent_Identity=30.6818181818182, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI2367200, Length=199, Percent_Identity=35.1758793969849, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324037, Length=108, Percent_Identity=42.5925925925926, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 27948; Mature: 27948

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQEGLKSVAGTLHTPEVHEDSGCFHLVDKPVDWTSFDVVAKIRNTYKLIGQKRKVGHCG
CCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
TLDPKASGLLILATGRKTKEIASLELLDKVYEGSFKLGVVTDSHDRETPEYNQCATGHLE
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHCCCCC
REQIIEAAASFVGTRQQQPPMYSAAWHNGKRLYELARKGDVVHDRKAKEIIIRRFEITAV
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ELPLVRFILEVSKGAYIRVIAHELGALLGVGAYLASLKRVAIGPYELGSACSVPETIEAI
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LKKRESVLFIEK
HHCCCCEEEEEC
>Mature Secondary Structure
MDQEGLKSVAGTLHTPEVHEDSGCFHLVDKPVDWTSFDVVAKIRNTYKLIGQKRKVGHCG
CCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
TLDPKASGLLILATGRKTKEIASLELLDKVYEGSFKLGVVTDSHDRETPEYNQCATGHLE
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHCCCCC
REQIIEAAASFVGTRQQQPPMYSAAWHNGKRLYELARKGDVVHDRKAKEIIIRRFEITAV
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ELPLVRFILEVSKGAYIRVIAHELGALLGVGAYLASLKRVAIGPYELGSACSVPETIEAI
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LKKRESVLFIEK
HHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA