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The map label for this gene is fruA [H]

Identifier: 119356202

GI number: 119356202

Start: 407105

End: 407575

Strand: Direct

Name: fruA [H]

Synonym: Cpha266_0363

Alternate gene names: 119356202

Gene position: 407105-407575 (Clockwise)

Preceding gene: 119356201

Following gene: 119356203

Centisome position: 12.99

GC content: 47.56

Gene sequence:

>471_bases
ATGAAAATTGAAAGCCTTCTGTCGGAGAGCTTTATTGCCTTGAATCTCGACCTTGCCTCAAAGAGTCAGGTGATTGATGC
CATGCTTGATCTTGTTGCAACTCATCCAAGTGTTTGCGATAAGGAGAAGCTGCGTCAGGATGTGCTTAAACGGGAGCGGG
AGATGTCAACAGGTATCGGAAAAGCAATTGCACTTCCTCATGCCAAAACAAGTGCGGTAAAACATCCGGTGCTTGCTCTT
GCAACACTCCATAAAGAGATCAATTTCGATTCCATCGATAACGAGCCTGTCAGGATTATTTTTCTTCTTGCAACTCCTGA
GGAGATGCTTGCCGAGCACTTGAAGCTGCTTGGCAGAATTACTCGCCTTGCCGGCAGGATGGATTTTCGCGAACAGTTGC
TGTTGCTGAAAACGCCGGCAGCGGTGCTTGACCTTTTCAGGGAAGAGGAAAAGGATTTCCCGCAGATCTGA

Upstream 100 bases:

>100_bases
TGCCCGGAAATAAAGTTTTTTTATATTATCCATTGAGTTAAGGTGAGAACAGCTCTGCTTGTGCAGCTGTAAATAGATTC
AACAACAATATGTTGTCACC

Downstream 100 bases:

>100_bases
AGGTGTTGACGGTGAGTCTTGAATTTGAGATTTTGCAGGTTGAGGTGATGAGATTCCTCCTTAAGTTCGGGATGACAAAT
AAACTCGGCATGCAGCGCTT

Product: putative PTS IIA-like nitrogen-regulatory protein PtsN

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKIESLLSESFIALNLDLASKSQVIDAMLDLVATHPSVCDKEKLRQDVLKREREMSTGIGKAIALPHAKTSAVKHPVLAL
ATLHKEINFDSIDNEPVRIIFLLATPEEMLAEHLKLLGRITRLAGRMDFREQLLLLKTPAAVLDLFREEEKDFPQI

Sequences:

>Translated_156_residues
MKIESLLSESFIALNLDLASKSQVIDAMLDLVATHPSVCDKEKLRQDVLKREREMSTGIGKAIALPHAKTSAVKHPVLAL
ATLHKEINFDSIDNEPVRIIFLLATPEEMLAEHLKLLGRITRLAGRMDFREQLLLLKTPAAVLDLFREEEKDFPQI
>Mature_156_residues
MKIESLLSESFIALNLDLASKSQVIDAMLDLVATHPSVCDKEKLRQDVLKREREMSTGIGKAIALPHAKTSAVKHPVLAL
ATLHKEINFDSIDNEPVRIIFLLATPEEMLAEHLKLLGRITRLAGRMDFREQLLLLKTPAAVLDLFREEEKDFPQI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786951, Length=145, Percent_Identity=28.9655172413793, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI2367327, Length=127, Percent_Identity=27.5590551181102, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1788726, Length=147, Percent_Identity=25.1700680272109, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17549; Mature: 17549

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIESLLSESFIALNLDLASKSQVIDAMLDLVATHPSVCDKEKLRQDVLKREREMSTGIG
CCHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
KAIALPHAKTSAVKHPVLALATLHKEINFDSIDNEPVRIIFLLATPEEMLAEHLKLLGRI
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHH
TRLAGRMDFREQLLLLKTPAAVLDLFREEEKDFPQI
HHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKIESLLSESFIALNLDLASKSQVIDAMLDLVATHPSVCDKEKLRQDVLKREREMSTGIG
CCHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
KAIALPHAKTSAVKHPVLALATLHKEINFDSIDNEPVRIIFLLATPEEMLAEHLKLLGRI
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHH
TRLAGRMDFREQLLLLKTPAAVLDLFREEEKDFPQI
HHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]