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The map label for this gene is dapF [H]

Identifier: 119356065

GI number: 119356065

Start: 240597

End: 241397

Strand: Reverse

Name: dapF [H]

Synonym: Cpha266_0218

Alternate gene names: 119356065

Gene position: 241397-240597 (Counterclockwise)

Preceding gene: 119356069

Following gene: 119356064

Centisome position: 7.7

GC content: 47.69

Gene sequence:

>801_bases
ATGCAGATCAATTTCAGCAAACTCTCCGGAGCAGGAAATGATTTTATCATTATCGATAACAGAGCGCTTGCAATCCATCT
TACTCAGCCGCAGATCAATGCCTTGTGTACTCGAAGAACAGGTGTTGGTGCAGACGGACTGATTCTTGTTGAACCCTCGT
TGCACTGCGATTTCACCATGAAATACTTTAATGCCGACGGATTTCCCGGATCAATGTGCGGCAATGGCGGGCGATGTGCT
GTTTATTTTGCCTTTATGCAGGGAGTTACGTCTGCCGAGCCTGAACATTTTTCCTTCGAGGCAAACAGTAACCGATATGA
CGCAGAGGTAACGAATTCCGAAACCGTCCGCCTGCACATGCTGGACGCTCGGGATTTCCGCTACCATATGGAAATTGAAG
GATTTGCCTGCACCTTCGTTAACACTGGGTCTCCTCATGTCGTCATCTTCGCCGACGACATTAATGCCATCAATGTTGCT
GAAAAAGGAGTCGCTATCAGACGCAATACGGATTTTTTCCCGGAAGGTACAAATGTTAACTTCGTCGAAATAACCTCACC
TTCAAGTATCGCTCTCAGAACTTTTGAACGAGGTGTTGAAGAGGAAACTCTCGCATGCGGCACCGGCGCAGTCGCTGCTG
CGATTATCAGCCGAAAACTTGGAAAAATCCTGCCAAACTCCGTTAAGGTTACGGTAAAAAGTGGCGATATGCTTGAGGTG
AGCTTCAGTGAAAACATGCAGAATATTACGCTGACCGGACCGGCAAAAATAGTCTACCAGGGCTCGGTCACGATCCCATG
A

Upstream 100 bases:

>100_bases
GTATACTTTACAATATAAGTGCTTGATGCGTATAGGGTTCCCATCCACCCCTGCCGTATAATTACAGAAAGAAAAGCAAA
CGTTTAACACTCAGAACACC

Downstream 100 bases:

>100_bases
TCGAACAACCTTTTTCCTGACTTGTTCTGCTGAATGTTTGAAAGCAGCCTCTCTCATTGGTACCGTTTACTGAAGGAGAA
AATGCAGGTTGAATAAAAAT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MQINFSKLSGAGNDFIIIDNRALAIHLTQPQINALCTRRTGVGADGLILVEPSLHCDFTMKYFNADGFPGSMCGNGGRCA
VYFAFMQGVTSAEPEHFSFEANSNRYDAEVTNSETVRLHMLDARDFRYHMEIEGFACTFVNTGSPHVVIFADDINAINVA
EKGVAIRRNTDFFPEGTNVNFVEITSPSSIALRTFERGVEEETLACGTGAVAAAIISRKLGKILPNSVKVTVKSGDMLEV
SFSENMQNITLTGPAKIVYQGSVTIP

Sequences:

>Translated_266_residues
MQINFSKLSGAGNDFIIIDNRALAIHLTQPQINALCTRRTGVGADGLILVEPSLHCDFTMKYFNADGFPGSMCGNGGRCA
VYFAFMQGVTSAEPEHFSFEANSNRYDAEVTNSETVRLHMLDARDFRYHMEIEGFACTFVNTGSPHVVIFADDINAINVA
EKGVAIRRNTDFFPEGTNVNFVEITSPSSIALRTFERGVEEETLACGTGAVAAAIISRKLGKILPNSVKVTVKSGDMLEV
SFSENMQNITLTGPAKIVYQGSVTIP
>Mature_266_residues
MQINFSKLSGAGNDFIIIDNRALAIHLTQPQINALCTRRTGVGADGLILVEPSLHCDFTMKYFNADGFPGSMCGNGGRCA
VYFAFMQGVTSAEPEHFSFEANSNRYDAEVTNSETVRLHMLDARDFRYHMEIEGFACTFVNTGSPHVVIFADDINAINVA
EKGVAIRRNTDFFPEGTNVNFVEITSPSSIALRTFERGVEEETLACGTGAVAAAIISRKLGKILPNSVKVTVKSGDMLEV
SFSENMQNITLTGPAKIVYQGSVTIP

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=280, Percent_Identity=32.8571428571429, Blast_Score=131, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 28892; Mature: 28892

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQINFSKLSGAGNDFIIIDNRALAIHLTQPQINALCTRRTGVGADGLILVEPSLHCDFTM
CEEECHHHCCCCCCEEEECCCEEEEEECCCCHHEEEEECCCCCCCCEEEECCCCCCEEEE
KYFNADGFPGSMCGNGGRCAVYFAFMQGVTSAEPEHFSFEANSNRYDAEVTNSETVRLHM
EEEECCCCCCCCCCCCCEEEEEEEEHHCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEE
LDARDFRYHMEIEGFACTFVNTGSPHVVIFADDINAINVAEKGVAIRRNTDFFPEGTNVN
EECCCEEEEEEECCEEEEEEECCCCEEEEEECCCCEEEECCCCEEEEECCCCCCCCCCEE
FVEITSPSSIALRTFERGVEEETLACGTGAVAAAIISRKLGKILPNSVKVTVKSGDMLEV
EEEEECCCCEEHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEE
SFSENMQNITLTGPAKIVYQGSVTIP
EECCCCCEEEEECCEEEEEECEEECC
>Mature Secondary Structure
MQINFSKLSGAGNDFIIIDNRALAIHLTQPQINALCTRRTGVGADGLILVEPSLHCDFTM
CEEECHHHCCCCCCEEEECCCEEEEEECCCCHHEEEEECCCCCCCCEEEECCCCCCEEEE
KYFNADGFPGSMCGNGGRCAVYFAFMQGVTSAEPEHFSFEANSNRYDAEVTNSETVRLHM
EEEECCCCCCCCCCCCCEEEEEEEEHHCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEE
LDARDFRYHMEIEGFACTFVNTGSPHVVIFADDINAINVAEKGVAIRRNTDFFPEGTNVN
EECCCEEEEEEECCEEEEEEECCCCEEEEEECCCCEEEECCCCEEEEECCCCCCCCCCEE
FVEITSPSSIALRTFERGVEEETLACGTGAVAAAIISRKLGKILPNSVKVTVKSGDMLEV
EEEEECCCCEEHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEE
SFSENMQNITLTGPAKIVYQGSVTIP
EECCCCCEEEEECCEEEEEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]