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The map label for this gene is idnO [C]

Identifier: 119356036

GI number: 119356036

Start: 214842

End: 215615

Strand: Direct

Name: idnO [C]

Synonym: Cpha266_0189

Alternate gene names: 119356036

Gene position: 214842-215615 (Clockwise)

Preceding gene: 119356035

Following gene: 119356040

Centisome position: 6.86

GC content: 47.16

Gene sequence:

>774_bases
ATGAAAAACATCGTTATCACAGGAAGCAGCAGAGGAATTGGCCTGGGTCTTGCTCATGCATTTCTCTCAAAAGGCTGCCG
TGTCATGATCAGCAGCAGCAACAGCAGCAACCTTGACAAAGCACTGAATGAGCTGGTCGAGCAGCATGGCTCCGAACATA
TCTCGGCAAAAACATGTGATGTTGCCAATTACCAGGAGGTTCAGGATCTTTGGGACCATGCGCAAAAATCGATAGGCCAG
GTAGATATCTGGATAAACAACGCCGGTATTGCCCACCCTTTGCTTCCGTTCTGGAAGCTTGATATGACACAGATCGGGCA
TACCCTTGATGTCAATCTTGCCGGAACAATAAATGGATCGCATGTGGCTATCAGAGGAATGATCGAACAGGGGTCGGGAT
ACCTGTACAATCTTGAAGGCCAGGGTGCCGATGGGGGAATAATTCACGGAATGGGTGTTTTTGGAACGTCCAAGGCGGCT
GTACACTATTTCTCGAAATCCCTTATCGAAGAAACCCGGTCTTTGAAGATAAAGGTAGGTACAATCATACCGGGAATCAT
ACGTACGGAGCTGCAAGCTAAAACCTCACAACAAACCGATGCTGGCAGGCTTTTTCTTTCGTTGCTTGGCGAAAATGTTC
GGGAAGCAACGGAAGACCTTGCAAAAAGAATTCTTGCAAACAATACACATGGGGTCTGCATCAACCGCATGACGACACCC
GACATGATCGGAAAAATCGCAACATTACCTATCAATTTTTTGTTAGGCAGGTGA

Upstream 100 bases:

>100_bases
TTGTGCCGACCCATATCTATGACCTTCTTTCTCAATATGACCTTTCATCGATACAAAGAATAATCGAGGCCCGGATTTAA
CAGCAAAAACTGATATAAAC

Downstream 100 bases:

>100_bases
CCTGACAGCGATGGAAATAGCAATCGGATAAGGGCAGGCAATCACAAGATAAAAGCCTGATAATTCCGGTTCCTGGCTTC
AAGTCACCTAAACGCGTGCG

Product: short-chain dehydrogenase/reductase SDR

Products: D-Gluconate; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKNIVITGSSRGIGLGLAHAFLSKGCRVMISSSNSSNLDKALNELVEQHGSEHISAKTCDVANYQEVQDLWDHAQKSIGQ
VDIWINNAGIAHPLLPFWKLDMTQIGHTLDVNLAGTINGSHVAIRGMIEQGSGYLYNLEGQGADGGIIHGMGVFGTSKAA
VHYFSKSLIEETRSLKIKVGTIIPGIIRTELQAKTSQQTDAGRLFLSLLGENVREATEDLAKRILANNTHGVCINRMTTP
DMIGKIATLPINFLLGR

Sequences:

>Translated_257_residues
MKNIVITGSSRGIGLGLAHAFLSKGCRVMISSSNSSNLDKALNELVEQHGSEHISAKTCDVANYQEVQDLWDHAQKSIGQ
VDIWINNAGIAHPLLPFWKLDMTQIGHTLDVNLAGTINGSHVAIRGMIEQGSGYLYNLEGQGADGGIIHGMGVFGTSKAA
VHYFSKSLIEETRSLKIKVGTIIPGIIRTELQAKTSQQTDAGRLFLSLLGENVREATEDLAKRILANNTHGVCINRMTTP
DMIGKIATLPINFLLGR
>Mature_257_residues
MKNIVITGSSRGIGLGLAHAFLSKGCRVMISSSNSSNLDKALNELVEQHGSEHISAKTCDVANYQEVQDLWDHAQKSIGQ
VDIWINNAGIAHPLLPFWKLDMTQIGHTLDVNLAGTINGSHVAIRGMIEQGSGYLYNLEGQGADGGIIHGMGVFGTSKAA
VHYFSKSLIEETRSLKIKVGTIIPGIIRTELQAKTSQQTDAGRLFLSLLGENVREATEDLAKRILANNTHGVCINRMTTP
DMIGKIATLPINFLLGR

Specific function: Catalyzes A Reversible Reduction Of 5-Ketoglutanate To Form D-Gluconate. Dependent On Nadp, Almost Inactive With NAD. [C]

COG id: COG4221

COG function: function code R; Short-chain alcohol dehydrogenase of unknown specificity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=222, Percent_Identity=29.2792792792793, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI7705925, Length=195, Percent_Identity=26.1538461538462, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI7705855, Length=201, Percent_Identity=25.3731343283582, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI31542939, Length=216, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790717, Length=192, Percent_Identity=28.6458333333333, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787335, Length=205, Percent_Identity=26.3414634146341, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082100, Length=248, Percent_Identity=24.1935483870968, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787820, Length=184, Percent_Identity=24.4565217391304, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1787905, Length=212, Percent_Identity=27.8301886792453, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI87082160, Length=228, Percent_Identity=26.3157894736842, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25147288, Length=192, Percent_Identity=28.125, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322227, Length=197, Percent_Identity=25.3807106598985, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21358495, Length=256, Percent_Identity=27.34375, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24584744, Length=262, Percent_Identity=27.4809160305344, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI28574402, Length=262, Percent_Identity=27.4809160305344, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21356301, Length=186, Percent_Identity=26.3440860215054, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116007236, Length=203, Percent_Identity=27.0935960591133, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24641232, Length=233, Percent_Identity=26.6094420600858, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24665243, Length=207, Percent_Identity=28.5024154589372, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI221331218, Length=207, Percent_Identity=28.5024154589372, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.69 [C]

Molecular weight: Translated: 27755; Mature: 27755

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIVITGSSRGIGLGLAHAFLSKGCRVMISSSNSSNLDKALNELVEQHGSEHISAKTCD
CCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCC
VANYQEVQDLWDHAQKSIGQVDIWINNAGIAHPLLPFWKLDMTQIGHTLDVNLAGTINGS
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCC
HVAIRGMIEQGSGYLYNLEGQGADGGIIHGMGVFGTSKAAVHYFSKSLIEETRSLKIKVG
EEEEEEEEECCCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCEEEEEC
TIIPGIIRTELQAKTSQQTDAGRLFLSLLGENVREATEDLAKRILANNTHGVCINRMTTP
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCH
DMIGKIATLPINFLLGR
HHHHHHHHCCHHHCCCC
>Mature Secondary Structure
MKNIVITGSSRGIGLGLAHAFLSKGCRVMISSSNSSNLDKALNELVEQHGSEHISAKTCD
CCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCC
VANYQEVQDLWDHAQKSIGQVDIWINNAGIAHPLLPFWKLDMTQIGHTLDVNLAGTINGS
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCC
HVAIRGMIEQGSGYLYNLEGQGADGGIIHGMGVFGTSKAAVHYFSKSLIEETRSLKIKVG
EEEEEEEEECCCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCEEEEEC
TIIPGIIRTELQAKTSQQTDAGRLFLSLLGENVREATEDLAKRILANNTHGVCINRMTTP
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCH
DMIGKIATLPINFLLGR
HHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH; NADP+; NADPH [C]

Metal ions: Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 20 {D-gluconate}} 0.006 {NADPH}} 0.9 {5-keto-D-gluconate}} 0.02 {NADP+}} [C]

Substrates: 5-Dehydro-D-gluconate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: 5-Dehydro-D-gluconate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced <==> D-Gluconate + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: Cd2+; Cs2+; Cu2+; Divalent metal ions; Hg2+; Ni2+; p-Chloromercuribenzoate; Sn2+; Sulfhydryl reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]