Click here to switch to the map view.

The map label for this gene is fhlA [H]

Identifier: 119355947

GI number: 119355947

Start: 112186

End: 114285

Strand: Direct

Name: fhlA [H]

Synonym: Cpha266_0097

Alternate gene names: 119355947

Gene position: 112186-114285 (Clockwise)

Preceding gene: 119355940

Following gene: 119355954

Centisome position: 3.58

GC content: 53.81

Gene sequence:

>2100_bases
ATGCAGAACATTGCCGTACACGAAGCCAGCACGTTGATGCGCTATATCAGCAACGCAATCGGTTCGATTCGCGATCCGCA
GGAGCTGTTTCGTACGGTGACCGACAAGCTGCGCCTCGTTTTCGAGTTCGATTCTGCCTTCATCATCACGCTGGACCGCG
ATCGGCGCTTTATCAATGTTGTTTTTGAGATGGTTCGCTTTGATCTTCCCGATACTATTCAGCGCAAAAAACGACCGGTT
GCCGGTTCGTGGATCGAGTCTCATCTTGGCGACGAAGGCGTTTCCGTGGTGGCTATTGAAGGAGAGATGGATCGTTATCC
CGCTGATTTTCCCGTTCCCCGGATGCTCTACGATCTCGGCATGCGGCAGGTGGTGCTCTCTCCGCTCCGTTCAGGAGGAA
AGGTGATCGGGTTTCTCAATTTTGTGTCGAGGGAGCAAAAACAGTGGAAAGAGAGCGATAAAGAGCTGCTCAGCACCCTT
TCATCCCCTGTTGCCGTTGCTCTCAGCAGCGCGCTTGCCTATGAGGAGCTGCGTCAGCGCGAGGCGCAGACCGCCATGCA
GCTTGCGGTGAACAACGCTCTTCTGACCATCAAGGATCGCAGCCGTATGCTTCTTGCCGTCTGCGAGCAGATCGACAAGC
TGGTACCCTGTACCTTTCTTGGTATTCGGGTGATGGACAGAGACGGAAACTTCAGGATTTTTGACAATTTCATGCGCGAA
ACGCCTTCAGGTTTTTTCCCTGTTTCTGCTCATGAAAAGCTTGATGTTCCGGATTTCGCGGAGATGGCCCGCGAGAGTTT
TTCGCTGATTACAAGCCCGGGCTGTTTTTCGGGAGAGGCTTTTCAGCTTCTCTGCGACAAGTATCGTCTTGTTGGGCTGG
TTCGGGAGAAATTCAATATCTGTTCGCTCATCAGCGTTCGACTCTGGGATCTTCCGGGAAGCTGCGCGGGTTTGATTATT
TCTGATACTTCGCGGGCTTTTGACGAGCATGATCAGGAAACCGTAGGCCTGATTGTGCCCCAGCTCTCTCTGGCCCTGCA
GAACTACCTTGCTTTTGAGGAGATTGACAAGCTTCGTCGCAAGCTCGAAGGGGAAAAAGCTTATCTTTTTGAGGAAATCA
AGGCGACGCATGATTTTGAGGAGATCAAGGGGGAGAGCGCCGTTCTTGCATCGGTACTGCACAGGGTAAGTCAGGTTGCG
CCTACCGATGCGACGGTTCTTATTCAGGGCGAGACGGGAACCGGCAAGGAGCTTTTTGCACGGGCGCTTCACAACCTCTC
CCGGCGCAGGAACCGGATGCTTGTCAAGGTGAATTGCGCCGCTCTTCCGGCAACGCTTATCGAGTCCGAGTTGTTCGGGC
ACGAGAAAGGGAGTTTTACCGGCGCTATCGAGAGGCGTATCGGGAAGTTCGAACTTGCCGAGGGAGGGACTGTTTTTCTT
GACGAGGTGGGCGAACTTCCCCTCGAACTTCAGGCAAAGCTCCTGAGGGTTCTGCAGGAGCGGGAGCTGGAGCGTCTCGG
GGGCAGGGGCGTGATAAGCGTCGATGTTCGAGTTCTGGCTGCAACGAACAGGAACCTGGAAAAGGAGGTGGCTGAGGGGC
GGTTTCGTGAAGATCTGTTTTTCCGGCTCAATGTTTTTCCTCTTTTCGTTCCGCCGCTGCGGGAACGCAGGGAGGATATT
CCCCTGCTGGCGCTTTATTTTGCGGCAAAATTTGCAAGATTGATGGGTAAGCCGCATTGCGAGTTCAGGGAGAGCGACAT
GCTGCTTCTCAAGGCGCGTGAGTGGAAGGGGAATATCAGGGAGCTTGCCCATCTTGTTGAGCAGGCGGTGATTGTTTCCG
ATGGCCGCTGGCTTGATTTTTCAACAATGCTCTCTCCTTCCCTTGCGTTTACGGAGCCTCGTGAGCAGGGGCAGCTCAAA
AGTATGCGGGAGTTCGAAGAGCAGGTCGCCATGATGGAGCGCGAGCTTATTCTTGAGGTTCTCAGTCAGTCGAACGGGCG
CGTAAGCGGTGAGGGAGGAGCGGCTGAAAAGCTCGGGATGCATCCTAAAACGCTCTATTCGCGTATTGAAAAGCTTGAAA
TCAGAAAACGGTATCGTTGA

Upstream 100 bases:

>100_bases
CGCTTTTACCTGCTTGCTCGTTGCTTTTCTGAAAAAATAAGAATATTCTGTAAGGAGTGTTCTTATTTTTCAGGAAATCC
CTTGTTAGCCGTTGCAACCT

Downstream 100 bases:

>100_bases
ACTGACGGGATCAGTTTTTTTCCCCCGCCGCCGAGCCGTTCTGTTTGAGACCGTTGGTATCGGGAGGCGGGATTTTCGAT
GCAAGTACTTTATCAATCCT

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MQNIAVHEASTLMRYISNAIGSIRDPQELFRTVTDKLRLVFEFDSAFIITLDRDRRFINVVFEMVRFDLPDTIQRKKRPV
AGSWIESHLGDEGVSVVAIEGEMDRYPADFPVPRMLYDLGMRQVVLSPLRSGGKVIGFLNFVSREQKQWKESDKELLSTL
SSPVAVALSSALAYEELRQREAQTAMQLAVNNALLTIKDRSRMLLAVCEQIDKLVPCTFLGIRVMDRDGNFRIFDNFMRE
TPSGFFPVSAHEKLDVPDFAEMARESFSLITSPGCFSGEAFQLLCDKYRLVGLVREKFNICSLISVRLWDLPGSCAGLII
SDTSRAFDEHDQETVGLIVPQLSLALQNYLAFEEIDKLRRKLEGEKAYLFEEIKATHDFEEIKGESAVLASVLHRVSQVA
PTDATVLIQGETGTGKELFARALHNLSRRRNRMLVKVNCAALPATLIESELFGHEKGSFTGAIERRIGKFELAEGGTVFL
DEVGELPLELQAKLLRVLQERELERLGGRGVISVDVRVLAATNRNLEKEVAEGRFREDLFFRLNVFPLFVPPLRERREDI
PLLALYFAAKFARLMGKPHCEFRESDMLLLKAREWKGNIRELAHLVEQAVIVSDGRWLDFSTMLSPSLAFTEPREQGQLK
SMREFEEQVAMMERELILEVLSQSNGRVSGEGGAAEKLGMHPKTLYSRIEKLEIRKRYR

Sequences:

>Translated_699_residues
MQNIAVHEASTLMRYISNAIGSIRDPQELFRTVTDKLRLVFEFDSAFIITLDRDRRFINVVFEMVRFDLPDTIQRKKRPV
AGSWIESHLGDEGVSVVAIEGEMDRYPADFPVPRMLYDLGMRQVVLSPLRSGGKVIGFLNFVSREQKQWKESDKELLSTL
SSPVAVALSSALAYEELRQREAQTAMQLAVNNALLTIKDRSRMLLAVCEQIDKLVPCTFLGIRVMDRDGNFRIFDNFMRE
TPSGFFPVSAHEKLDVPDFAEMARESFSLITSPGCFSGEAFQLLCDKYRLVGLVREKFNICSLISVRLWDLPGSCAGLII
SDTSRAFDEHDQETVGLIVPQLSLALQNYLAFEEIDKLRRKLEGEKAYLFEEIKATHDFEEIKGESAVLASVLHRVSQVA
PTDATVLIQGETGTGKELFARALHNLSRRRNRMLVKVNCAALPATLIESELFGHEKGSFTGAIERRIGKFELAEGGTVFL
DEVGELPLELQAKLLRVLQERELERLGGRGVISVDVRVLAATNRNLEKEVAEGRFREDLFFRLNVFPLFVPPLRERREDI
PLLALYFAAKFARLMGKPHCEFRESDMLLLKAREWKGNIRELAHLVEQAVIVSDGRWLDFSTMLSPSLAFTEPREQGQLK
SMREFEEQVAMMERELILEVLSQSNGRVSGEGGAAEKLGMHPKTLYSRIEKLEIRKRYR
>Mature_699_residues
MQNIAVHEASTLMRYISNAIGSIRDPQELFRTVTDKLRLVFEFDSAFIITLDRDRRFINVVFEMVRFDLPDTIQRKKRPV
AGSWIESHLGDEGVSVVAIEGEMDRYPADFPVPRMLYDLGMRQVVLSPLRSGGKVIGFLNFVSREQKQWKESDKELLSTL
SSPVAVALSSALAYEELRQREAQTAMQLAVNNALLTIKDRSRMLLAVCEQIDKLVPCTFLGIRVMDRDGNFRIFDNFMRE
TPSGFFPVSAHEKLDVPDFAEMARESFSLITSPGCFSGEAFQLLCDKYRLVGLVREKFNICSLISVRLWDLPGSCAGLII
SDTSRAFDEHDQETVGLIVPQLSLALQNYLAFEEIDKLRRKLEGEKAYLFEEIKATHDFEEIKGESAVLASVLHRVSQVA
PTDATVLIQGETGTGKELFARALHNLSRRRNRMLVKVNCAALPATLIESELFGHEKGSFTGAIERRIGKFELAEGGTVFL
DEVGELPLELQAKLLRVLQERELERLGGRGVISVDVRVLAATNRNLEKEVAEGRFREDLFFRLNVFPLFVPPLRERREDI
PLLALYFAAKFARLMGKPHCEFRESDMLLLKAREWKGNIRELAHLVEQAVIVSDGRWLDFSTMLSPSLAFTEPREQGQLK
SMREFEEQVAMMERELILEVLSQSNGRVSGEGGAAEKLGMHPKTLYSRIEKLEIRKRYR

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=594, Percent_Identity=37.7104377104377, Blast_Score=338, Evalue=8e-94,
Organism=Escherichia coli, GI87082117, Length=391, Percent_Identity=45.7800511508951, Blast_Score=323, Evalue=3e-89,
Organism=Escherichia coli, GI1788550, Length=331, Percent_Identity=45.9214501510574, Blast_Score=272, Evalue=5e-74,
Organism=Escherichia coli, GI1790437, Length=308, Percent_Identity=46.4285714285714, Blast_Score=261, Evalue=1e-70,
Organism=Escherichia coli, GI1789233, Length=326, Percent_Identity=42.3312883435583, Blast_Score=256, Evalue=4e-69,
Organism=Escherichia coli, GI87082152, Length=305, Percent_Identity=45.2459016393443, Blast_Score=235, Evalue=8e-63,
Organism=Escherichia coli, GI1788905, Length=276, Percent_Identity=44.5652173913043, Blast_Score=226, Evalue=5e-60,
Organism=Escherichia coli, GI1790299, Length=236, Percent_Identity=47.4576271186441, Blast_Score=219, Evalue=6e-58,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=48.7068965517241, Blast_Score=199, Evalue=7e-52,
Organism=Escherichia coli, GI1786524, Length=329, Percent_Identity=39.5136778115502, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1787583, Length=248, Percent_Identity=40.7258064516129, Blast_Score=177, Evalue=3e-45,
Organism=Escherichia coli, GI1789828, Length=258, Percent_Identity=36.8217054263566, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87081858, Length=320, Percent_Identity=29.6875, Blast_Score=126, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 79389; Mature: 79389

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNIAVHEASTLMRYISNAIGSIRDPQELFRTVTDKLRLVFEFDSAFIITLDRDRRFINV
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECCEEEEEECCCCHHHHH
VFEMVRFDLPDTIQRKKRPVAGSWIESHLGDEGVSVVAIEGEMDRYPADFPVPRMLYDLG
HHHHHHCCCCHHHHHHCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
MRQVVLSPLRSGGKVIGFLNFVSREQKQWKESDKELLSTLSSPVAVALSSALAYEELRQR
HHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EAQTAMQLAVNNALLTIKDRSRMLLAVCEQIDKLVPCTFLGIRVMDRDGNFRIFDNFMRE
HHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCHHEEEEEEEECCCCEEHHHHHHHH
TPSGFFPVSAHEKLDVPDFAEMARESFSLITSPGCFSGEAFQLLCDKYRLVGLVREKFNI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
CSLISVRLWDLPGSCAGLIISDTSRAFDEHDQETVGLIVPQLSLALQNYLAFEEIDKLRR
HHHHHHHEECCCCCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLEGEKAYLFEEIKATHDFEEIKGESAVLASVLHRVSQVAPTDATVLIQGETGTGKELFA
HHCCCHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
RALHNLSRRRNRMLVKVNCAALPATLIESELFGHEKGSFTGAIERRIGKFELAEGGTVFL
HHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCCEEEE
DEVGELPLELQAKLLRVLQERELERLGGRGVISVDVRVLAATNRNLEKEVAEGRFREDLF
CHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHCCCHHHHHE
FRLNVFPLFVPPLRERREDIPLLALYFAAKFARLMGKPHCEFRESDMLLLKAREWKGNIR
EEEEEHEEECCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHCCCHH
ELAHLVEQAVIVSDGRWLDFSTMLSPSLAFTEPREQGQLKSMREFEEQVAMMERELILEV
HHHHHHHHHHEECCCCEEEHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHH
LSQSNGRVSGEGGAAEKLGMHPKTLYSRIEKLEIRKRYR
HHCCCCEECCCCCCHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQNIAVHEASTLMRYISNAIGSIRDPQELFRTVTDKLRLVFEFDSAFIITLDRDRRFINV
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECCEEEEEECCCCHHHHH
VFEMVRFDLPDTIQRKKRPVAGSWIESHLGDEGVSVVAIEGEMDRYPADFPVPRMLYDLG
HHHHHHCCCCHHHHHHCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
MRQVVLSPLRSGGKVIGFLNFVSREQKQWKESDKELLSTLSSPVAVALSSALAYEELRQR
HHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EAQTAMQLAVNNALLTIKDRSRMLLAVCEQIDKLVPCTFLGIRVMDRDGNFRIFDNFMRE
HHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCHHEEEEEEEECCCCEEHHHHHHHH
TPSGFFPVSAHEKLDVPDFAEMARESFSLITSPGCFSGEAFQLLCDKYRLVGLVREKFNI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
CSLISVRLWDLPGSCAGLIISDTSRAFDEHDQETVGLIVPQLSLALQNYLAFEEIDKLRR
HHHHHHHEECCCCCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLEGEKAYLFEEIKATHDFEEIKGESAVLASVLHRVSQVAPTDATVLIQGETGTGKELFA
HHCCCHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
RALHNLSRRRNRMLVKVNCAALPATLIESELFGHEKGSFTGAIERRIGKFELAEGGTVFL
HHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCCEEEE
DEVGELPLELQAKLLRVLQERELERLGGRGVISVDVRVLAATNRNLEKEVAEGRFREDLF
CHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHCCCHHHHHE
FRLNVFPLFVPPLRERREDIPLLALYFAAKFARLMGKPHCEFRESDMLLLKAREWKGNIR
EEEEEHEEECCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHCCCHH
ELAHLVEQAVIVSDGRWLDFSTMLSPSLAFTEPREQGQLKSMREFEEQVAMMERELILEV
HHHHHHHHHHEECCCCEEEHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHH
LSQSNGRVSGEGGAAEKLGMHPKTLYSRIEKLEIRKRYR
HHCCCCEECCCCCCHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]