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The map label for this gene is mjaIM [H]

Identifier: 119355923

GI number: 119355923

Start: 83897

End: 85186

Strand: Direct

Name: mjaIM [H]

Synonym: Cpha266_0073

Alternate gene names: 119355923

Gene position: 83897-85186 (Clockwise)

Preceding gene: 119355920

Following gene: 119355926

Centisome position: 2.68

GC content: 51.63

Gene sequence:

>1290_bases
ATGAATCTGCTGCCGGACAGTTCGGTGCATCTTATCATTACCTCTCCCCCCTATTGGCAGCTCAAGGACTACGGGACGGA
GAACCAGATCGGATTTCACGACAGCTATGAGAGCTACATCAACAATCTGAACCTTGTCTGGAGCGAGTGCGAGCGGGTGC
TGCATCCCGGCTGCAGGCTCTGCATCAACATCGGCGACCAGTTCGCCCGTTCGGTGTATTACGGACGGTACAAGGTTATC
CCGATCCGGACGGAGATCATCAGGTTCTGCGAGACTATCGGTTTCGACTATATGGGCGCGGTGATCTGGCAGAAGGTGAC
GACAACCAACACCACCGGAGGCGCTTCCATTATGGGAAGCTTTCCGTATCCGCGCAACGGCATTCTCAAGCTCGATTATG
AGTTCATTCTTCTGTTCAAAAAACAGGGAGATGCCCCGAAGCCGTCAAAAGAGCTGAAAGAGCGCTCAGCCATGACCACC
GAAGAGTGGAACACCTGTTTCTCCGGACACTGGAACTTTGCCGGAGCAAAACAGGATGGCCATATCGCCGTGTTTCCGGA
AGATCTGCCGCATCGCCTGATCAGGATGTTCGCTTTCGTCGGCGAAACGGTGCTCGATCCGTTCATGGGCAGCGGGACGA
CCAGTCTTGCGGCAAAAAACCTCGACAGGAACTCGGTCGGCTACGAAATCAATCCCGAGTTTATCGGGATAGCAAGAGAG
AAACTCAATTCCAACCAGACGGACTTTGCCGGAACGGAGTATGTTTTTCAGCACGATACCCTGAAGGGGGATATTTCCGA
AATGATCGAGCGTCTTCCTTATCGTTTTCAAGACCCCCACAAACTCGACAAGAAAATCGACCCGCGAAAGCTGACGTTCG
GTTCAAGAGTAGAAAAGGGTGGCGGGGCAAAACAGGAAGAGACGTTTATCGTCAGGGAGATTCTAAGCCCCGAGATGGTC
AGATTGTCCAACGGCCTGACGGTGAGGCTGATCGGAGTGAAGGAAGACCCTTTCACGCGGGAAAAAGCTGTCGGGTATCT
CGTTGACAAGATCAAGGGAAAACGGATTTTCATGAAGTACGACAGCATGAAATACGATGGGCGGAACAATCTGCTCTGTT
ACCTCTATCTGGAAAACAGGACATTTGTCAACGCGCATCTGATCAGGAGTGGTTTAGTAGGGATTGACGGCAGCTACGAG
TACAGGTACAAACAGAAATTCCTGGCTTTTTCTGATGAGAACAGCACACTTGTTCAAACAGGTCGGTGGTCTGGAATTGC
TGCAAAGTGA

Upstream 100 bases:

>100_bases
AAATCCGGAAAAATATCTATACTTCTTTCATTCGATCCTTTAACCCAATTGCAAACCATTGAAAACCCGTCACACCATAA
TAGAAGGCGACAGCAGGCAG

Downstream 100 bases:

>100_bases
AGATGTTGAACATGCAGGATACATGCAGGGGTTGTACTCCCTGTTTCAATATTTAATCCGGCATGGGAATGACCTTGTCA
AGCCCGGTTTCCACAAGAAA

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.MjaI; N-4 cytosine-specific methyltransferase MjaI [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNLLPDSSVHLIITSPPYWQLKDYGTENQIGFHDSYESYINNLNLVWSECERVLHPGCRLCINIGDQFARSVYYGRYKVI
PIRTEIIRFCETIGFDYMGAVIWQKVTTTNTTGGASIMGSFPYPRNGILKLDYEFILLFKKQGDAPKPSKELKERSAMTT
EEWNTCFSGHWNFAGAKQDGHIAVFPEDLPHRLIRMFAFVGETVLDPFMGSGTTSLAAKNLDRNSVGYEINPEFIGIARE
KLNSNQTDFAGTEYVFQHDTLKGDISEMIERLPYRFQDPHKLDKKIDPRKLTFGSRVEKGGGAKQEETFIVREILSPEMV
RLSNGLTVRLIGVKEDPFTREKAVGYLVDKIKGKRIFMKYDSMKYDGRNNLLCYLYLENRTFVNAHLIRSGLVGIDGSYE
YRYKQKFLAFSDENSTLVQTGRWSGIAAK

Sequences:

>Translated_429_residues
MNLLPDSSVHLIITSPPYWQLKDYGTENQIGFHDSYESYINNLNLVWSECERVLHPGCRLCINIGDQFARSVYYGRYKVI
PIRTEIIRFCETIGFDYMGAVIWQKVTTTNTTGGASIMGSFPYPRNGILKLDYEFILLFKKQGDAPKPSKELKERSAMTT
EEWNTCFSGHWNFAGAKQDGHIAVFPEDLPHRLIRMFAFVGETVLDPFMGSGTTSLAAKNLDRNSVGYEINPEFIGIARE
KLNSNQTDFAGTEYVFQHDTLKGDISEMIERLPYRFQDPHKLDKKIDPRKLTFGSRVEKGGGAKQEETFIVREILSPEMV
RLSNGLTVRLIGVKEDPFTREKAVGYLVDKIKGKRIFMKYDSMKYDGRNNLLCYLYLENRTFVNAHLIRSGLVGIDGSYE
YRYKQKFLAFSDENSTLVQTGRWSGIAAK
>Mature_429_residues
MNLLPDSSVHLIITSPPYWQLKDYGTENQIGFHDSYESYINNLNLVWSECERVLHPGCRLCINIGDQFARSVYYGRYKVI
PIRTEIIRFCETIGFDYMGAVIWQKVTTTNTTGGASIMGSFPYPRNGILKLDYEFILLFKKQGDAPKPSKELKERSAMTT
EEWNTCFSGHWNFAGAKQDGHIAVFPEDLPHRLIRMFAFVGETVLDPFMGSGTTSLAAKNLDRNSVGYEINPEFIGIARE
KLNSNQTDFAGTEYVFQHDTLKGDISEMIERLPYRFQDPHKLDKKIDPRKLTFGSRVEKGGGAKQEETFIVREILSPEMV
RLSNGLTVRLIGVKEDPFTREKAVGYLVDKIKGKRIFMKYDSMKYDGRNNLLCYLYLENRTFVNAHLIRSGLVGIDGSYE
YRYKQKFLAFSDENSTLVQTGRWSGIAAK

Specific function: This methylase recognizes the double-stranded sequence CTAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MjaI endonuclease (Potential) [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 49070; Mature: 49070

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLPDSSVHLIITSPPYWQLKDYGTENQIGFHDSYESYINNLNLVWSECERVLHPGCRL
CCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCEE
CINIGDQFARSVYYGRYKVIPIRTEIIRFCETIGFDYMGAVIWQKVTTTNTTGGASIMGS
EEECHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCHHHHHHHHHHHEECCCCCCCCEEEEC
FPYPRNGILKLDYEFILLFKKQGDAPKPSKELKERSAMTTEEWNTCFSGHWNFAGAKQDG
CCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHCCCHHHHCHHCCCCCCCCCCCCCC
HIAVFPEDLPHRLIRMFAFVGETVLDPFMGSGTTSLAAKNLDRNSVGYEINPEFIGIARE
EEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEECCHHEEHHHH
KLNSNQTDFAGTEYVFQHDTLKGDISEMIERLPYRFQDPHKLDKKIDPRKLTFGSRVEKG
HCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCEECCCCCCCCC
GGAKQEETFIVREILSPEMVRLSNGLTVRLIGVKEDPFTREKAVGYLVDKIKGKRIFMKY
CCCCCHHHHHHHHHCCCHHEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEE
DSMKYDGRNNLLCYLYLENRTFVNAHLIRSGLVGIDGSYEYRYKQKFLAFSDENSTLVQT
CCEEECCCCCEEEEEEECCCEEEEHHHHHCCCEECCCCCCEEEEEEEEEECCCCCEEEEC
GRWSGIAAK
CCCCCCCCC
>Mature Secondary Structure
MNLLPDSSVHLIITSPPYWQLKDYGTENQIGFHDSYESYINNLNLVWSECERVLHPGCRL
CCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCEE
CINIGDQFARSVYYGRYKVIPIRTEIIRFCETIGFDYMGAVIWQKVTTTNTTGGASIMGS
EEECHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCHHHHHHHHHHHEECCCCCCCCEEEEC
FPYPRNGILKLDYEFILLFKKQGDAPKPSKELKERSAMTTEEWNTCFSGHWNFAGAKQDG
CCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHCCCHHHHCHHCCCCCCCCCCCCCC
HIAVFPEDLPHRLIRMFAFVGETVLDPFMGSGTTSLAAKNLDRNSVGYEINPEFIGIARE
EEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEECCHHEEHHHH
KLNSNQTDFAGTEYVFQHDTLKGDISEMIERLPYRFQDPHKLDKKIDPRKLTFGSRVEKG
HCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCEECCCCCCCCC
GGAKQEETFIVREILSPEMVRLSNGLTVRLIGVKEDPFTREKAVGYLVDKIKGKRIFMKY
CCCCCHHHHHHHHHCCCHHEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEE
DSMKYDGRNNLLCYLYLENRTFVNAHLIRSGLVGIDGSYEYRYKQKFLAFSDENSTLVQT
CCEEECCCCCEEEEEEECCCEEEEHHHHHCCCEECCCCCCEEEEEEEEEECCCCCEEEEC
GRWSGIAAK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]