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The map label for this gene is trmE

Identifier: 118582021

GI number: 118582021

Start: 4003875

End: 4005245

Strand: Reverse

Name: trmE

Synonym: Ppro_3622

Alternate gene names: 118582021

Gene position: 4005245-4003875 (Counterclockwise)

Preceding gene: 118582022

Following gene: 118582020

Centisome position: 99.93

GC content: 59.45

Gene sequence:

>1371_bases
ATGTACATGAGGGATACCATAGCGGCAATCAGCACTCCCCCCGGCAATGGCGGTATCGGCATAATCCGGATCAGCGGCGG
GAGTGCATCCTGCATAGCTGAAACCCTCTTCAGACCGGTGCGCAGCGGTGGCCTCATCAGCCACCGTTTTTATTATGGCA
CGCTGATCGACATTCAGACCGGCGCGGTTGTGGACGAGGTTATGGCGGTCATCATGCGCGCCCCCCACTCCTATACGCGG
GAGGATGTGCTGGAACTGCACTGCCATGGCGGCATGCTGGTTGTTGAGCGGATTCTGTCGCTGGTCCTCCGCGCAGGGGC
GCGTCTGGCTGAACCGGGAGAGTTTACCCGCCGGGCTTTCCTCAATGGCAGGATCGATCTCGTCCAGGCAGAGTCTGTCA
TGGATATCATCTCTGCCCAGACCGATGCCGCGCTTGCGCTGGCCCAACGCCAGCGGGGAGGCGTTCTCTCTTCGCGCATC
GCCGAGGCACGTCAACTGCTGCTGCATGCGCTGGCACTGATCGAGGCATACATCGATTTTCCCGAGGACGATCTGGGTGA
AACGGATGTCGAGAGCATCCGTTCATCCGTCGAGGGGGCACGGACCCACATTCGGCGCCTGCTTGAGGATTTCAACGAGG
GGCGTCTCCTGCGCGAGGGAATATCGGTCCTGATCATCGGGAAACCGAATGCCGGCAAGTCAAGCCTGCTCAACCGTCTG
CTCAACGAGAATCGGGCCATCGTTACCCACCTTCCCGGCACCACCCGCGACATTATCGAGGAGACGATCAATCTTGGGGG
ACTTGCGGTTCGTCTCCTGGATACGGCGGGAATTCGCCATACCGAGGATGTGATTGAGCAGGAGGGGATCAACCGTGCAC
TGGAGAAAATTCCCCAGGCCGACCTGATCCTGTTCGTTCTGGACGGTTCGCGCCCCTTTGGCCCGGAAGACCGGCTTATC
CTCGATGCCCTGCAGGGAAACAGGTTTGTTGCGGCCATTAGCAAGGCCGACCTTCCCCGCGTTCTTGAACTGCCGTCCGA
GTGCTGCAATTTGCCGCAGGTCTCTTTTTCCGCCGAGTCGGGTGAAGGGGTCGATGATCTCAAGAGCACTGTCCGATCAT
TGTTCGTCTCCAGTCAGGCCATCGATAACCGGGAGTATGTCGCCATTTCACGGGTGCGGCACCGCGATGCGCTGAGCTCC
GCGGATGGGTCTCTGTCCAGATTCCGGCAGGGGCTTGATTCAGGAGCTTCTCTTGACCTTCTGGCGCTGGATCTTCGGGA
GGCGCTGGCGTCAGTGGGTGAGGTGACGGGACAGGTGACGAACGAAGACGTTCTCGATCTGATCTTTTCTTCCTTCTGTA
TCGGCAAGTAG

Upstream 100 bases:

>100_bases
GTCCTGTACTGGTTGCTGAACAACATACTCACCATTGGCCAGCAGATGTACATCAATAAACTGGTTAACGACTGATCCCG
GTCGTGGCGGAGTAGCGTCA

Downstream 100 bases:

>100_bases
GTTGCCACAACCGCAGCATTTAGGGTCATGTTTCACGTGAAACGTCGGAGATGTAATTCATGATGACGTACGATTTTGAA
TACGATGTAATCGTGGTTGG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MYMRDTIAAISTPPGNGGIGIIRISGGSASCIAETLFRPVRSGGLISHRFYYGTLIDIQTGAVVDEVMAVIMRAPHSYTR
EDVLELHCHGGMLVVERILSLVLRAGARLAEPGEFTRRAFLNGRIDLVQAESVMDIISAQTDAALALAQRQRGGVLSSRI
AEARQLLLHALALIEAYIDFPEDDLGETDVESIRSSVEGARTHIRRLLEDFNEGRLLREGISVLIIGKPNAGKSSLLNRL
LNENRAIVTHLPGTTRDIIEETINLGGLAVRLLDTAGIRHTEDVIEQEGINRALEKIPQADLILFVLDGSRPFGPEDRLI
LDALQGNRFVAAISKADLPRVLELPSECCNLPQVSFSAESGEGVDDLKSTVRSLFVSSQAIDNREYVAISRVRHRDALSS
ADGSLSRFRQGLDSGASLDLLALDLREALASVGEVTGQVTNEDVLDLIFSSFCIGK

Sequences:

>Translated_456_residues
MYMRDTIAAISTPPGNGGIGIIRISGGSASCIAETLFRPVRSGGLISHRFYYGTLIDIQTGAVVDEVMAVIMRAPHSYTR
EDVLELHCHGGMLVVERILSLVLRAGARLAEPGEFTRRAFLNGRIDLVQAESVMDIISAQTDAALALAQRQRGGVLSSRI
AEARQLLLHALALIEAYIDFPEDDLGETDVESIRSSVEGARTHIRRLLEDFNEGRLLREGISVLIIGKPNAGKSSLLNRL
LNENRAIVTHLPGTTRDIIEETINLGGLAVRLLDTAGIRHTEDVIEQEGINRALEKIPQADLILFVLDGSRPFGPEDRLI
LDALQGNRFVAAISKADLPRVLELPSECCNLPQVSFSAESGEGVDDLKSTVRSLFVSSQAIDNREYVAISRVRHRDALSS
ADGSLSRFRQGLDSGASLDLLALDLREALASVGEVTGQVTNEDVLDLIFSSFCIGK
>Mature_456_residues
MYMRDTIAAISTPPGNGGIGIIRISGGSASCIAETLFRPVRSGGLISHRFYYGTLIDIQTGAVVDEVMAVIMRAPHSYTR
EDVLELHCHGGMLVVERILSLVLRAGARLAEPGEFTRRAFLNGRIDLVQAESVMDIISAQTDAALALAQRQRGGVLSSRI
AEARQLLLHALALIEAYIDFPEDDLGETDVESIRSSVEGARTHIRRLLEDFNEGRLLREGISVLIIGKPNAGKSSLLNRL
LNENRAIVTHLPGTTRDIIEETINLGGLAVRLLDTAGIRHTEDVIEQEGINRALEKIPQADLILFVLDGSRPFGPEDRLI
LDALQGNRFVAAISKADLPRVLELPSECCNLPQVSFSAESGEGVDDLKSTVRSLFVSSQAIDNREYVAISRVRHRDALSS
ADGSLSRFRQGLDSGASLDLLALDLREALASVGEVTGQVTNEDVLDLIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=35.9913793103448, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI193082988, Length=496, Percent_Identity=34.2741935483871, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI193082993, Length=453, Percent_Identity=35.0993377483444, Blast_Score=215, Evalue=6e-56,
Organism=Escherichia coli, GI2367268, Length=468, Percent_Identity=37.6068376068376, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI87082120, Length=173, Percent_Identity=31.7919075144509, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788919, Length=166, Percent_Identity=33.1325301204819, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=464, Percent_Identity=31.6810344827586, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6323665, Length=499, Percent_Identity=33.8677354709419, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI45550813, Length=487, Percent_Identity=32.2381930184805, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_PELPD (A1AV43)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_903271.1
- ProteinModelPortal:   A1AV43
- SMR:   A1AV43
- STRING:   A1AV43
- GeneID:   4574422
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3622
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   A1AV43
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49480; Mature: 49480

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 85-85 BINDING 124-124 BINDING 456-456

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMRDTIAAISTPPGNGGIGIIRISGGSASCIAETLFRPVRSGGLISHRFYYGTLIDIQT
CCCCHHHHHEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECC
GAVVDEVMAVIMRAPHSYTREDVLELHCHGGMLVVERILSLVLRAGARLAEPGEFTRRAF
CHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LNGRIDLVQAESVMDIISAQTDAALALAQRQRGGVLSSRIAEARQLLLHALALIEAYIDF
HCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PEDDLGETDVESIRSSVEGARTHIRRLLEDFNEGRLLREGISVLIIGKPNAGKSSLLNRL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEECCCCCHHHHHHHH
LNENRAIVTHLPGTTRDIIEETINLGGLAVRLLDTAGIRHTEDVIEQEGINRALEKIPQA
HCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
DLILFVLDGSRPFGPEDRLILDALQGNRFVAAISKADLPRVLELPSECCNLPQVSFSAES
CEEEEEEECCCCCCCCCCEEHHHHCCCEEEEEECCCCCCHHHHCCHHHCCCCCCEECCCC
GEGVDDLKSTVRSLFVSSQAIDNREYVAISRVRHRDALSSADGSLSRFRQGLDSGASLDL
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHH
LALDLREALASVGEVTGQVTNEDVLDLIFSSFCIGK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYMRDTIAAISTPPGNGGIGIIRISGGSASCIAETLFRPVRSGGLISHRFYYGTLIDIQT
CCCCHHHHHEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECC
GAVVDEVMAVIMRAPHSYTREDVLELHCHGGMLVVERILSLVLRAGARLAEPGEFTRRAF
CHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LNGRIDLVQAESVMDIISAQTDAALALAQRQRGGVLSSRIAEARQLLLHALALIEAYIDF
HCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PEDDLGETDVESIRSSVEGARTHIRRLLEDFNEGRLLREGISVLIIGKPNAGKSSLLNRL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEECCCCCHHHHHHHH
LNENRAIVTHLPGTTRDIIEETINLGGLAVRLLDTAGIRHTEDVIEQEGINRALEKIPQA
HCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
DLILFVLDGSRPFGPEDRLILDALQGNRFVAAISKADLPRVLELPSECCNLPQVSFSAES
CEEEEEEECCCCCCCCCCEEHHHHCCCEEEEEECCCCCCHHHHCCHHHCCCCCCEECCCC
GEGVDDLKSTVRSLFVSSQAIDNREYVAISRVRHRDALSSADGSLSRFRQGLDSGASLDL
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHH
LALDLREALASVGEVTGQVTNEDVLDLIFSSFCIGK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA