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The map label for this gene is pit [H]

Identifier: 118581984

GI number: 118581984

Start: 3964726

End: 3965727

Strand: Direct

Name: pit [H]

Synonym: Ppro_3585

Alternate gene names: 118581984

Gene position: 3964726-3965727 (Clockwise)

Preceding gene: 118581983

Following gene: 118581986

Centisome position: 98.92

GC content: 62.67

Gene sequence:

>1002_bases
ATGCTTGAGTCGAGCTTTGTGATGCTCTGCCTGGTCATAGCCGCCGCGCTGGCCTTCGACTACATCAACGGCTTTCACGA
CACGGCCAACGCCATAGCCACCTGCGTCTCCACCCGGGCCCTCTCCATCAAGGCCGCCATTTTCATGGCAGCCACCCTGA
ACTTCGCCGGCGCCATGGTCTCCACCAAGGTGGCCTCCACCATCGGCAAGGGGATCGTGGACGGCGCCAACATCACCCAG
ATGGTAGTGCTGGCCGGTATCCTGGGGGCGATTATCTGGGATCTGGTCACCTGGTACTATGGACTCCCCTCCTCCTCCTC
TCACGCCATTATCGGCGGCATCATGGGCTCGGTATTCGCCCACGCCGGCCTGGCTGCCCTGAAGTTGGACGGACTCAAGA
TCATCGTCCTGGCCCTGGTGCTCTCCCCCATCCTGGGCACCCTGATCGGGTTCCTGTTCATGCTGGCCATCTACTGGACA
TTCAGGAACAGCGCCCCCGGCGGCCTCAACAAGGTTTTCCGCCACCTGCAGGTGGCCTCGGCCGCCTTCATGGCCTTTTC
CCACGGCATGGCCGACGCCCAGAAATCCATGGGCGTGATTACCCTGGCCCTTGTCAGCTACGGATTTCTCAAGACATTCT
CCGTTCCCTGGGAAGTCATGGTGGCCTGCGCAACGGCCATGGCCCTGGGTACGGCCGGTGGAGGATGGCGCATCATCAAG
ACCGTGGGGCGTGACTTCGTCAAGCTGCAACCGGTGCACGGCTTCTGCGTCGAAACCGCATCGGCCGGCGTCATCATGGG
CGCCGCCGCCTTCGGCATGCCCACCAGCACAACCCATGTGATCACGTCCACCATCCTGGGGGTGGGGCTCTCCAAGCGGA
TTACCGCGGTCAACTGGAAGGTGGCCCAGCGCATCGTTATCGCCTGGGTCCTGACCATCCCGGCCTCCGCCTTGGTCTCC
TACCTCACCTACCAGGTACTGAGCCCGATCCTGGGCAAGTAG

Upstream 100 bases:

>100_bases
TTCCAGCTGATCAAGTGGAAGGAGATCTACGAAACCCTGGAGAAAGCCACCGACAAGTGCGAAGACGCCGCCAATATCCT
GGAAAGCGTGGTGGTAAAGA

Downstream 100 bases:

>100_bases
AACTGGGGACGGGCCCCCGGGTCCGTCCTACTCCAACTCCTCTCCCACCTCTTCCATCTCCCGGGTCAACTCGGCCAGAG
TAGCATCCTTGCGCCGGGAA

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MLESSFVMLCLVIAAALAFDYINGFHDTANAIATCVSTRALSIKAAIFMAATLNFAGAMVSTKVASTIGKGIVDGANITQ
MVVLAGILGAIIWDLVTWYYGLPSSSSHAIIGGIMGSVFAHAGLAALKLDGLKIIVLALVLSPILGTLIGFLFMLAIYWT
FRNSAPGGLNKVFRHLQVASAAFMAFSHGMADAQKSMGVITLALVSYGFLKTFSVPWEVMVACATAMALGTAGGGWRIIK
TVGRDFVKLQPVHGFCVETASAGVIMGAAAFGMPTSTTHVITSTILGVGLSKRITAVNWKVAQRIVIAWVLTIPASALVS
YLTYQVLSPILGK

Sequences:

>Translated_333_residues
MLESSFVMLCLVIAAALAFDYINGFHDTANAIATCVSTRALSIKAAIFMAATLNFAGAMVSTKVASTIGKGIVDGANITQ
MVVLAGILGAIIWDLVTWYYGLPSSSSHAIIGGIMGSVFAHAGLAALKLDGLKIIVLALVLSPILGTLIGFLFMLAIYWT
FRNSAPGGLNKVFRHLQVASAAFMAFSHGMADAQKSMGVITLALVSYGFLKTFSVPWEVMVACATAMALGTAGGGWRIIK
TVGRDFVKLQPVHGFCVETASAGVIMGAAAFGMPTSTTHVITSTILGVGLSKRITAVNWKVAQRIVIAWVLTIPASALVS
YLTYQVLSPILGK
>Mature_333_residues
MLESSFVMLCLVIAAALAFDYINGFHDTANAIATCVSTRALSIKAAIFMAATLNFAGAMVSTKVASTIGKGIVDGANITQ
MVVLAGILGAIIWDLVTWYYGLPSSSSHAIIGGIMGSVFAHAGLAALKLDGLKIIVLALVLSPILGTLIGFLFMLAIYWT
FRNSAPGGLNKVFRHLQVASAAFMAFSHGMADAQKSMGVITLALVSYGFLKTFSVPWEVMVACATAMALGTAGGGWRIIK
TVGRDFVKLQPVHGFCVETASAGVIMGAAAFGMPTSTTHVITSTILGVGLSKRITAVNWKVAQRIVIAWVLTIPASALVS
YLTYQVLSPILGK

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI5803173, Length=152, Percent_Identity=30.9210526315789, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI31543630, Length=152, Percent_Identity=29.6052631578947, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1789907, Length=220, Percent_Identity=35.4545454545455, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789360, Length=221, Percent_Identity=34.841628959276, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17539280, Length=172, Percent_Identity=30.8139534883721, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71981576, Length=156, Percent_Identity=31.4102564102564, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17536725, Length=185, Percent_Identity=28.1081081081081, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25146401, Length=155, Percent_Identity=29.0322580645161, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI32566716, Length=155, Percent_Identity=27.0967741935484, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17557328, Length=155, Percent_Identity=27.0967741935484, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319773, Length=156, Percent_Identity=31.4102564102564, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356511, Length=180, Percent_Identity=31.6666666666667, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 34841; Mature: 34841

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLESSFVMLCLVIAAALAFDYINGFHDTANAIATCVSTRALSIKAAIFMAATLNFAGAMV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STKVASTIGKGIVDGANITQMVVLAGILGAIIWDLVTWYYGLPSSSSHAIIGGIMGSVFA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
HAGLAALKLDGLKIIVLALVLSPILGTLIGFLFMLAIYWTFRNSAPGGLNKVFRHLQVAS
HCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
AAFMAFSHGMADAQKSMGVITLALVSYGFLKTFSVPWEVMVACATAMALGTAGGGWRIIK
HHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHH
TVGRDFVKLQPVHGFCVETASAGVIMGAAAFGMPTSTTHVITSTILGVGLSKRITAVNWK
HHCCHHEEECCCCHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
VAQRIVIAWVLTIPASALVSYLTYQVLSPILGK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLESSFVMLCLVIAAALAFDYINGFHDTANAIATCVSTRALSIKAAIFMAATLNFAGAMV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STKVASTIGKGIVDGANITQMVVLAGILGAIIWDLVTWYYGLPSSSSHAIIGGIMGSVFA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
HAGLAALKLDGLKIIVLALVLSPILGTLIGFLFMLAIYWTFRNSAPGGLNKVFRHLQVAS
HCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
AAFMAFSHGMADAQKSMGVITLALVSYGFLKTFSVPWEVMVACATAMALGTAGGGWRIIK
HHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHH
TVGRDFVKLQPVHGFCVETASAGVIMGAAAFGMPTSTTHVITSTILGVGLSKRITAVNWK
HHCCHHEEECCCCHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
VAQRIVIAWVLTIPASALVSYLTYQVLSPILGK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]