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The map label for this gene is cofH [H]

Identifier: 118581977

GI number: 118581977

Start: 3958032

End: 3959111

Strand: Direct

Name: cofH [H]

Synonym: Ppro_3578

Alternate gene names: 118581977

Gene position: 3958032-3959111 (Clockwise)

Preceding gene: 118581976

Following gene: 118581978

Centisome position: 98.75

GC content: 63.89

Gene sequence:

>1080_bases
ATGCCCACCCTGGAAACAATAAAAGCCAACATCCGTGAAAACCGCCGCATCTCCGCCCAGGAGGCGCTGGCCCTGTTCGC
CTCCAACGACCTGCTGGCCATGGGCGAGATGGCGGCCCTGGCCAACGAGCGCAAGAACGGCAAAAACGTTTTCTTCAACG
TCAACCGCCACATCAACCCCAGCAACATCTGCGTCAACCGCTGCGCCTTCTGCGCCTTCTACCGGACCGCCGACCAGGAG
GGGGCCTACACCCTGACCACGGAGGAGATCTGCCACAGGGCGGTGGAGGCCCAGGCCAGCGGCGCCACCGAACTGCACAT
CGTCGGCGGGCTGCATCCCGATCTCCCCTTCCCCTTTTACGAAGAAACCCTCTCCGCCCTGCGCCGCGCCGCGCCAGACC
TGCACATCAAGGCATTCACCGCCGTTGAGATCGACTACTTCAGCTGCATCTCGGGCCTCAGCCTGGAGCAGGTGCTGGAG
CGCTTGAAGGCTGCCGGACTCGGCTCCATGCCCGGCGGCGGCGCGGAGATCCTGGTGGAAGAGGTGCGCCGCAAAATCTG
CCCGGAGAAGATCTCCGGGGCGCGCTGGCTGGAGGTAATCGCCACTGCCCACCGTGCCGGACTGAAAACCAACGCCACCA
TGCTCTACGGCCATCTGGAAACGGTTGCCGACCGGGTGGCCCACATGGAGATGCTGCGCGACCTGCAAGACCGGACCAAC
GGTTTCCAGGCCTTCATCCCGCTGGCCTTCCAGCCGGAAAATTCTGACCTGAAGCTGGGCATCAGCGGTACCTCCGGCCT
GGACGACCTGAAGACCCTTGCCATCTCGCGCATTTTCCTGGACAATTTCGACCACATCAAGGCCTACTGGGTCATGCTGG
GGGAGAAGATCGCCCAGGTGTCGCTGGCCTTCGGGGTCAACGACCTGGACGGGACCGTGATCGAGGAGAAGATCGGCCAC
GACGCCGGTGCACCTTCCCCCCAGTCGCTGACCAAGAGCGCCATCATCCGCCTGATCAAACGGGCCGACAAGGTCCCGGT
GGAACGGGACACGCTCTACCGGCCGCTGGCCAGCTACTGA

Upstream 100 bases:

>100_bases
AAGGAAGCCGACCGGATCGTGAACAGGCTGGAGGGGGACTGAACGCCGTACCCTACAAATACCTTGAACCGGCGGGTATG
TTTGACGTAGACTTCCCCCC

Downstream 100 bases:

>100_bases
CGACAAACCGCATATTCCCACCACAAGAGCGCAGCAATCACGACGATATCATTTTTTTCGGATTTCCCTTCACCCGCCAA
AGATCCTGCTCTCCTGCGTA

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MPTLETIKANIRENRRISAQEALALFASNDLLAMGEMAALANERKNGKNVFFNVNRHINPSNICVNRCAFCAFYRTADQE
GAYTLTTEEICHRAVEAQASGATELHIVGGLHPDLPFPFYEETLSALRRAAPDLHIKAFTAVEIDYFSCISGLSLEQVLE
RLKAAGLGSMPGGGAEILVEEVRRKICPEKISGARWLEVIATAHRAGLKTNATMLYGHLETVADRVAHMEMLRDLQDRTN
GFQAFIPLAFQPENSDLKLGISGTSGLDDLKTLAISRIFLDNFDHIKAYWVMLGEKIAQVSLAFGVNDLDGTVIEEKIGH
DAGAPSPQSLTKSAIIRLIKRADKVPVERDTLYRPLASY

Sequences:

>Translated_359_residues
MPTLETIKANIRENRRISAQEALALFASNDLLAMGEMAALANERKNGKNVFFNVNRHINPSNICVNRCAFCAFYRTADQE
GAYTLTTEEICHRAVEAQASGATELHIVGGLHPDLPFPFYEETLSALRRAAPDLHIKAFTAVEIDYFSCISGLSLEQVLE
RLKAAGLGSMPGGGAEILVEEVRRKICPEKISGARWLEVIATAHRAGLKTNATMLYGHLETVADRVAHMEMLRDLQDRTN
GFQAFIPLAFQPENSDLKLGISGTSGLDDLKTLAISRIFLDNFDHIKAYWVMLGEKIAQVSLAFGVNDLDGTVIEEKIGH
DAGAPSPQSLTKSAIIRLIKRADKVPVERDTLYRPLASY
>Mature_358_residues
PTLETIKANIRENRRISAQEALALFASNDLLAMGEMAALANERKNGKNVFFNVNRHINPSNICVNRCAFCAFYRTADQEG
AYTLTTEEICHRAVEAQASGATELHIVGGLHPDLPFPFYEETLSALRRAAPDLHIKAFTAVEIDYFSCISGLSLEQVLER
LKAAGLGSMPGGGAEILVEEVRRKICPEKISGARWLEVIATAHRAGLKTNATMLYGHLETVADRVAHMEMLRDLQDRTNG
FQAFIPLAFQPENSDLKLGISGTSGLDDLKTLAISRIFLDNFDHIKAYWVMLGEKIAQVSLAFGVNDLDGTVIEEKIGHD
AGAPSPQSLTKSAIIRLIKRADKVPVERDTLYRPLASY

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39511; Mature: 39380

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLETIKANIRENRRISAQEALALFASNDLLAMGEMAALANERKNGKNVFFNVNRHINP
CCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCH
SNICVNRCAFCAFYRTADQEGAYTLTTEEICHRAVEAQASGATELHIVGGLHPDLPFPFY
HHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHH
EETLSALRRAAPDLHIKAFTAVEIDYFSCISGLSLEQVLERLKAAGLGSMPGGGAEILVE
HHHHHHHHHCCCCCEEEEEEEEEEHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
EVRRKICPEKISGARWLEVIATAHRAGLKTNATMLYGHLETVADRVAHMEMLRDLQDRTN
HHHHHHCHHHCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC
GFQAFIPLAFQPENSDLKLGISGTSGLDDLKTLAISRIFLDNFDHIKAYWVMLGEKIAQV
CEEEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SLAFGVNDLDGTVIEEKIGHDAGAPSPQSLTKSAIIRLIKRADKVPVERDTLYRPLASY
HHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PTLETIKANIRENRRISAQEALALFASNDLLAMGEMAALANERKNGKNVFFNVNRHINP
CCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCH
SNICVNRCAFCAFYRTADQEGAYTLTTEEICHRAVEAQASGATELHIVGGLHPDLPFPFY
HHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHH
EETLSALRRAAPDLHIKAFTAVEIDYFSCISGLSLEQVLERLKAAGLGSMPGGGAEILVE
HHHHHHHHHCCCCCEEEEEEEEEEHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
EVRRKICPEKISGARWLEVIATAHRAGLKTNATMLYGHLETVADRVAHMEMLRDLQDRTN
HHHHHHCHHHCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC
GFQAFIPLAFQPENSDLKLGISGTSGLDDLKTLAISRIFLDNFDHIKAYWVMLGEKIAQV
CEEEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SLAFGVNDLDGTVIEEKIGHDAGAPSPQSLTKSAIIRLIKRADKVPVERDTLYRPLASY
HHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]