Click here to switch to the map view.

The map label for this gene is nuoF2 [H]

Identifier: 118581916

GI number: 118581916

Start: 3888289

End: 3889755

Strand: Reverse

Name: nuoF2 [H]

Synonym: Ppro_3516

Alternate gene names: 118581916

Gene position: 3889755-3888289 (Counterclockwise)

Preceding gene: 118581917

Following gene: 118581914

Centisome position: 97.05

GC content: 60.33

Gene sequence:

>1467_bases
ATGGAACCCAAAAAGCAGCTGCGGATAGCGACCAGGAACTGCGGTTTCATCGATCCGGAAAATATCGAGGATTACATCGG
GGTGCGCGGCTACGAGGCGCTGGCCAGCGCCATTACCAACGGCACGCCTGCCGGAGTGATCGAGCAGATGAAAAAATCGG
GCTTGAGGGGGCGGGGTGGCGGCGGTTTCCCCACTGGCGTCAAGTGGGCATTTGCCGCCGCGAATCAGGCTGACCAGAAA
TACGTCGTCTGCAATGCGGACGAGGGGGACCCTGGCGCCTTCATGGATCGGGCCCTGCTGGAGGGGGATCCCCATTCCAT
AGTGGAGGCCATGGCCATCTGCGGCTACGCCATCGGCGCATCCATCGGCGTCGTCTACATCCGGGCCGAATACCCCCTAG
CAATCAAACGGCTGATCAAGTCCATCGAGGATGCCCGTGACTACGGCCTCCTGGGCAGGAACATTCTCGGTACCGGCTTC
AATTTCGACATAGAACTGAAGTATGGCGCCGGCGCCTTTGTCTGCGGCGAGGAGACCGCCCTGATCAGGTCCATGGAAGG
GAGCCGCGGCGAACCGTACACCAAGCCCCCCTTTCCGGCCAGCGCCGGGTACTGGGGCAAACCTACCATCGTCAACAACG
TGGAGACCCTTGCCAATATCCCCCCTATCCTCGTCAAGGGGGCTGACTGGTTCTCCTCCATCGGTACGGAGAATTCCAAG
GGGACCAAGGTCTTCGCCCTGGCCGGCAAGATCACGAACGTCGGCCTGATCGAGGTTCCCATGGGCATAACCCTGGGTGA
GATCATCTTCGAGATCGGCGGCGGCTGCCCGAATGGCAAACAGTTCAAGGCGGTGCAGACCGGCGGCCCCTCCGGCGGCG
CGCTGACCTCCAGGGATATCGATGTCCCCATCGACTATGACAATCTGATCGCCAGAGGCTCCATGATGGGCTCCGGCGGC
ATGATCGTCATGGATGAGGACAACTGCATGGTCGACGTGGCCAAGTTCTTCCTCGACTTCACCATGGATGAGACTTGCGG
CAAATGCACCCCCTGCCGCATCGGTTCAAAACGGCTCCATGAGATCCTCTGCCGGATCACCCAGGGCGAGGGCACACTGG
AGGATATGGAAAACCTGCAATGTCTCGGCGCAACCATCAAGGACACGGCGCTGTGCGGCTTCGGCCAGACCATGCCCAAC
CCGGTGCTCTCCACCATCAGGACCTTCATGGATGAATACCGTGCCCATGTAGTTGACAAGAAGTGCCCGGCCGGGGTCTG
TGTCAGCCTGCTGGAAATTGTCATCCTGGCCGAAAGCTGTGTCGGCTGCACCCTCTGCAGCCGTGTCTGCCCGGTCAACT
GCATCTCCGGTACGGTCAAAGGTGTGCATGTCATCGATCAGGCAGCCTGCATCAAGTGCGGCGCCTGCATCGACGCGTGC
AAATTCCAGGCGATAGTGAAACGATGA

Upstream 100 bases:

>100_bases
AACTTGTCCAGAAATATCTGAAAAAAGGCGAGCTTACCGGCACGGTGATTCCGGTGAACTACACCAGGGTAGAACTATGA
TGTCGGGGAGCAGATAGAGC

Downstream 100 bases:

>100_bases
CGGAAAGAGTCGTTGCGACAGGTCGGCTCAGGCACCTCACAGTAGCTTGCGCAGATACTGGCCGGTGTAGGAACCGCTCA
CCGTTGCCAGCTGCTCCGGG

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MEPKKQLRIATRNCGFIDPENIEDYIGVRGYEALASAITNGTPAGVIEQMKKSGLRGRGGGGFPTGVKWAFAAANQADQK
YVVCNADEGDPGAFMDRALLEGDPHSIVEAMAICGYAIGASIGVVYIRAEYPLAIKRLIKSIEDARDYGLLGRNILGTGF
NFDIELKYGAGAFVCGEETALIRSMEGSRGEPYTKPPFPASAGYWGKPTIVNNVETLANIPPILVKGADWFSSIGTENSK
GTKVFALAGKITNVGLIEVPMGITLGEIIFEIGGGCPNGKQFKAVQTGGPSGGALTSRDIDVPIDYDNLIARGSMMGSGG
MIVMDEDNCMVDVAKFFLDFTMDETCGKCTPCRIGSKRLHEILCRITQGEGTLEDMENLQCLGATIKDTALCGFGQTMPN
PVLSTIRTFMDEYRAHVVDKKCPAGVCVSLLEIVILAESCVGCTLCSRVCPVNCISGTVKGVHVIDQAACIKCGACIDAC
KFQAIVKR

Sequences:

>Translated_488_residues
MEPKKQLRIATRNCGFIDPENIEDYIGVRGYEALASAITNGTPAGVIEQMKKSGLRGRGGGGFPTGVKWAFAAANQADQK
YVVCNADEGDPGAFMDRALLEGDPHSIVEAMAICGYAIGASIGVVYIRAEYPLAIKRLIKSIEDARDYGLLGRNILGTGF
NFDIELKYGAGAFVCGEETALIRSMEGSRGEPYTKPPFPASAGYWGKPTIVNNVETLANIPPILVKGADWFSSIGTENSK
GTKVFALAGKITNVGLIEVPMGITLGEIIFEIGGGCPNGKQFKAVQTGGPSGGALTSRDIDVPIDYDNLIARGSMMGSGG
MIVMDEDNCMVDVAKFFLDFTMDETCGKCTPCRIGSKRLHEILCRITQGEGTLEDMENLQCLGATIKDTALCGFGQTMPN
PVLSTIRTFMDEYRAHVVDKKCPAGVCVSLLEIVILAESCVGCTLCSRVCPVNCISGTVKGVHVIDQAACIKCGACIDAC
KFQAIVKR
>Mature_488_residues
MEPKKQLRIATRNCGFIDPENIEDYIGVRGYEALASAITNGTPAGVIEQMKKSGLRGRGGGGFPTGVKWAFAAANQADQK
YVVCNADEGDPGAFMDRALLEGDPHSIVEAMAICGYAIGASIGVVYIRAEYPLAIKRLIKSIEDARDYGLLGRNILGTGF
NFDIELKYGAGAFVCGEETALIRSMEGSRGEPYTKPPFPASAGYWGKPTIVNNVETLANIPPILVKGADWFSSIGTENSK
GTKVFALAGKITNVGLIEVPMGITLGEIIFEIGGGCPNGKQFKAVQTGGPSGGALTSRDIDVPIDYDNLIARGSMMGSGG
MIVMDEDNCMVDVAKFFLDFTMDETCGKCTPCRIGSKRLHEILCRITQGEGTLEDMENLQCLGATIKDTALCGFGQTMPN
PVLSTIRTFMDEYRAHVVDKKCPAGVCVSLLEIVILAESCVGCTLCSRVCPVNCISGTVKGVHVIDQAACIKCGACIDAC
KFQAIVKR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=374, Percent_Identity=38.7700534759358, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI260656005, Length=374, Percent_Identity=38.7700534759358, Blast_Score=271, Evalue=1e-72,
Organism=Escherichia coli, GI1788620, Length=402, Percent_Identity=40.0497512437811, Blast_Score=323, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17531727, Length=377, Percent_Identity=39.2572944297082, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI19920794, Length=370, Percent_Identity=38.9189189189189, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI45550452, Length=367, Percent_Identity=39.5095367847411, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24653819, Length=386, Percent_Identity=32.6424870466321, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24653817, Length=347, Percent_Identity=31.4121037463977, Blast_Score=186, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 51931; Mature: 51931

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPKKQLRIATRNCGFIDPENIEDYIGVRGYEALASAITNGTPAGVIEQMKKSGLRGRGG
CCCHHHEEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
GGFPTGVKWAFAAANQADQKYVVCNADEGDPGAFMDRALLEGDPHSIVEAMAICGYAIGA
CCCCCCCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCC
SIGVVYIRAEYPLAIKRLIKSIEDARDYGLLGRNILGTGFNFDIELKYGAGAFVCGEETA
CEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEECCHHH
LIRSMEGSRGEPYTKPPFPASAGYWGKPTIVNNVETLANIPPILVKGADWFSSIGTENSK
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHCCCCEEEECCHHHHHCCCCCCC
GTKVFALAGKITNVGLIEVPMGITLGEIIFEIGGGCPNGKQFKAVQTGGPSGGALTSRDI
CCEEEEEECCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCC
DVPIDYDNLIARGSMMGSGGMIVMDEDNCMVDVAKFFLDFTMDETCGKCTPCRIGSKRLH
CCCCCHHHHHHCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
EILCRITQGEGTLEDMENLQCLGATIKDTALCGFGQTMPNPVLSTIRTFMDEYRAHVVDK
HHHHHHCCCCCCHHHHHHHHHHCCCHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
KCPAGVCVSLLEIVILAESCVGCTLCSRVCPVNCISGTVKGVHVIDQAACIKCGACIDAC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KFQAIVKR
HHHHHHCC
>Mature Secondary Structure
MEPKKQLRIATRNCGFIDPENIEDYIGVRGYEALASAITNGTPAGVIEQMKKSGLRGRGG
CCCHHHEEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
GGFPTGVKWAFAAANQADQKYVVCNADEGDPGAFMDRALLEGDPHSIVEAMAICGYAIGA
CCCCCCCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCC
SIGVVYIRAEYPLAIKRLIKSIEDARDYGLLGRNILGTGFNFDIELKYGAGAFVCGEETA
CEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEECCHHH
LIRSMEGSRGEPYTKPPFPASAGYWGKPTIVNNVETLANIPPILVKGADWFSSIGTENSK
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHCCCCEEEECCHHHHHCCCCCCC
GTKVFALAGKITNVGLIEVPMGITLGEIIFEIGGGCPNGKQFKAVQTGGPSGGALTSRDI
CCEEEEEECCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCC
DVPIDYDNLIARGSMMGSGGMIVMDEDNCMVDVAKFFLDFTMDETCGKCTPCRIGSKRLH
CCCCCHHHHHHCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
EILCRITQGEGTLEDMENLQCLGATIKDTALCGFGQTMPNPVLSTIRTFMDEYRAHVVDK
HHHHHHCCCCCCHHHHHHHHHHCCCHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
KCPAGVCVSLLEIVILAESCVGCTLCSRVCPVNCISGTVKGVHVIDQAACIKCGACIDAC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KFQAIVKR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]