Click here to switch to the map view.

The map label for this gene is truA

Identifier: 118581782

GI number: 118581782

Start: 3732723

End: 3733457

Strand: Reverse

Name: truA

Synonym: Ppro_3382

Alternate gene names: 118581782

Gene position: 3733457-3732723 (Counterclockwise)

Preceding gene: 118581783

Following gene: 118581779

Centisome position: 93.15

GC content: 66.8

Gene sequence:

>735_bases
ATGCGCACCATCAAACTGACCATAGAGTACGACGGCACCAATTACGCCGGCTGGCAGGTCCAGCCCAACGGCCTGGCCGT
GCAGCAGGTGCTCGAGGAGGCGTTGCTGCGGCTCCTGGGGGAGCGGGTGCGGCTGCGCTCTTCGGGACGCACCGATGCCG
GCGTGCATGCCCGCGCCATGGTGGCCTCTTTCACCACCAGCCGGTCGCTTCCCCTGCAGGCGTTCGTGGGCGGGGCCAAC
CGCTTCCTTCCCGACGACATCGCCATCCTCTCCGCGGCCGAGGCGGCGCCGGAGTTCAGGCCGATCCAGGACGCGCGAAG
CAAATGGTATCGCTACACCATCTACAACGCGCCGGTGCGCTCCCCTCTGCGGCGCCTGTTCAGCTGGCACGTGCGCGAGC
CGCTGGACACGGATGCCATGCAGCTGGCCGCGCATCATTTCCCGGGGCGCCACGACTTCGCCGCCTTCCGCGCCTCCAAC
TGCGCGGCAAAGACGACGCTGCGACGGATCGACTCCGTAACCATCGCCAGTGAAGGGGAACTGATCGTCATCGACGTGAT
CGGGGAGGGGTTTCTGAAGAACATGGTGCGGGTCATGGCGGGAACTCTGGTGGATGTGGGGCGGGGGAGGTTCGAGCCGG
AACGGGTCGCCTGGCTGCTGGAGAATCCGGACAGGAAAAAGGCCGGCGTGACGGCGCCGGCCTGCGGACTCTGCCTGATG
GATGTCAGCTACTGA

Upstream 100 bases:

>100_bases
GTCGTTGTTTGTACGTTATGTACCTTCTTTGCGGCTTGGCGTCTCTGCGTGAGATGAAGTGCCTGTTTCAGAATAATCAG
TTACAGGAAAGGGTCGCCCC

Downstream 100 bases:

>100_bases
TGCCGTCGTTCGGTGTTACTGCTCCGTGAGCAGCCGCCTGATCAGGAGCTCCATGGAGCGGCCGACCTCATCGTTGAAGT
TCCGGTAGATCTCTCGCACC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRTIKLTIEYDGTNYAGWQVQPNGLAVQQVLEEALLRLLGERVRLRSSGRTDAGVHARAMVASFTTSRSLPLQAFVGGAN
RFLPDDIAILSAAEAAPEFRPIQDARSKWYRYTIYNAPVRSPLRRLFSWHVREPLDTDAMQLAAHHFPGRHDFAAFRASN
CAAKTTLRRIDSVTIASEGELIVIDVIGEGFLKNMVRVMAGTLVDVGRGRFEPERVAWLLENPDRKKAGVTAPACGLCLM
DVSY

Sequences:

>Translated_244_residues
MRTIKLTIEYDGTNYAGWQVQPNGLAVQQVLEEALLRLLGERVRLRSSGRTDAGVHARAMVASFTTSRSLPLQAFVGGAN
RFLPDDIAILSAAEAAPEFRPIQDARSKWYRYTIYNAPVRSPLRRLFSWHVREPLDTDAMQLAAHHFPGRHDFAAFRASN
CAAKTTLRRIDSVTIASEGELIVIDVIGEGFLKNMVRVMAGTLVDVGRGRFEPERVAWLLENPDRKKAGVTAPACGLCLM
DVSY
>Mature_244_residues
MRTIKLTIEYDGTNYAGWQVQPNGLAVQQVLEEALLRLLGERVRLRSSGRTDAGVHARAMVASFTTSRSLPLQAFVGGAN
RFLPDDIAILSAAEAAPEFRPIQDARSKWYRYTIYNAPVRSPLRRLFSWHVREPLDTDAMQLAAHHFPGRHDFAAFRASN
CAAKTTLRRIDSVTIASEGELIVIDVIGEGFLKNMVRVMAGTLVDVGRGRFEPERVAWLLENPDRKKAGVTAPACGLCLM
DVSY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=268, Percent_Identity=31.7164179104478, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788657, Length=241, Percent_Identity=36.9294605809129, Blast_Score=151, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_PELPD (A1AUF4)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_903032.1
- ProteinModelPortal:   A1AUF4
- SMR:   A1AUF4
- STRING:   A1AUF4
- GeneID:   4572533
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3382
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   A1AUF4
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27098; Mature: 27098

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIKLTIEYDGTNYAGWQVQPNGLAVQQVLEEALLRLLGERVRLRSSGRTDAGVHARAM
CCEEEEEEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VASFTTSRSLPLQAFVGGANRFLPDDIAILSAAEAAPEFRPIQDARSKWYRYTIYNAPVR
HHHHHCCCCCCHHHEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCEEEEEEECCCHH
SPLRRLFSWHVREPLDTDAMQLAAHHFPGRHDFAAFRASNCAAKTTLRRIDSVTIASEGE
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHCEEEECCCC
LIVIDVIGEGFLKNMVRVMAGTLVDVGRGRFEPERVAWLLENPDRKKAGVTAPACGLCLM
EEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCCCCCCCCHHCEEEE
DVSY
ECCC
>Mature Secondary Structure
MRTIKLTIEYDGTNYAGWQVQPNGLAVQQVLEEALLRLLGERVRLRSSGRTDAGVHARAM
CCEEEEEEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VASFTTSRSLPLQAFVGGANRFLPDDIAILSAAEAAPEFRPIQDARSKWYRYTIYNAPVR
HHHHHCCCCCCHHHEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCEEEEEEECCCHH
SPLRRLFSWHVREPLDTDAMQLAAHHFPGRHDFAAFRASNCAAKTTLRRIDSVTIASEGE
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHCEEEECCCC
LIVIDVIGEGFLKNMVRVMAGTLVDVGRGRFEPERVAWLLENPDRKKAGVTAPACGLCLM
EEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCCCCCCCCHHCEEEE
DVSY
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA