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The map label for this gene is yfhA [H]

Identifier: 118581763

GI number: 118581763

Start: 3711253

End: 3712728

Strand: Reverse

Name: yfhA [H]

Synonym: Ppro_3363

Alternate gene names: 118581763

Gene position: 3712728-3711253 (Counterclockwise)

Preceding gene: 118581771

Following gene: 118581762

Centisome position: 92.63

GC content: 57.72

Gene sequence:

>1476_bases
ATGAATGAAATCAAATCGTTATCCGCGCCCGATCGAGAGTTTTTCAGGAACGTTTCCCAGGCAGCCTTCACCAACCCCTT
CAGCCAGTCACGCATCGAAGTTGACCGCAGGATCGCCGACGCCCCGCCCTCCATGTCGTGGGACGATACCGTTGAACAGG
CTGTCGCACGGGTCAGCGAGCGGCTGCGGATGCTGAAGCTGCGTGGCTCAACCGACCTGAGCGGCTACAGCCAGAGCGAC
CGGGAACTGTTGCGCACCGCGATGCTCTTCGACCTGTACCACCAGTACCGGCAACCCTTTGACGCCTTTATCCTGAAACA
GCTTACGGCAGGGGACACCCCCATCCCGGTCCCCTTCGCGTCAACAATCCTTTCCGCCCTTGCCAACATCGGCTTGAACA
GTGCTGCCGCGCAACGCTTTCTGGCCATCTTCTACCAGATCCGGAGAGCCTTCTACTTTATCGAAAGCGGGCTGACCGGC
CCCTCCCCGGCCATGCAGAACCTGCGCCTGCAACTCTGGAACAATATCTTCACCTGCAACATCACCTGGTACGAAGAGTA
TCTCTGGGACCGGATGGAAGATTTCTCGACCCTGCTGCTGGGCGAAACCGGCACCGGCAAGGGGGCCGCGGCTGCAGCCA
TCGGACGCTCCGGCTTCATCCCCTTCAATGAACGCAAGGGGTGCTTCAGCGAGAGCTTTACCCGCTCCTTTATTGCCATC
AACCTGTCACAGTATCCTGAATCCCTGCTGGAGTCGGAACTCTTCGGTCACAAAAAAGGCGCCTTTACCGGCGCCATCGA
ACATCACGAGGGGGTCTTCGACCGCTGTTCTCCCCACGGCTCCATTTTTCTGGATGAAATCGGCGATGTATCCATCCCGG
TGCAGATCAAGCTCCTGCAGGTATTGCAGGAACGAACATTCGCACCGGTGGGAAGTCACGAACGACGACGCTTCCAAGGG
CGGGTGATCGCGGCAACCAACCGACCGCTGGACGAGATGCGTGCCAAGGGGCAGTTCCGCAACGATTTCTACTACCGCCT
CTGTTCCGACATCGTAACGGTTCCCTCGCTCAGGGAGAGGATCAGGAAGGAATCCCGCGAACTGGAAGTGCTGATCAGCA
GGATTCTGGTCCGCATGGTCGGTGAAAAAGCCAGGGAATACACCTGTCTGGTATACGACATCATCAGGCGGGATGTGGGA
AGTGACTATGCCTGGCCGGGCAACGTGCGGGAGCTGGAACAGGCGGTGCGTAGAATCATCATTACCCGCCATTACAGGGG
TGACAGAACCCCGCCGAATCAGGAGAATGACGACAACAATCTTCTTGATGCCATCAGGAGCGAATCACTGGACGCCGAAT
CGCTGTTGGCAGGCTACTGTAGCGTGCTCTACCAACGTCACGGCACCTACGAAGAGGTGGCGCGAATCACGAACCTTGAC
CGCCGAACGGTCAAGAAATATATCCGGAAGGACTAA

Upstream 100 bases:

>100_bases
CCCACACCCTGGCACCGCCTGACCATGCCGGAGTCGCCGGCAGCAAGCACCGGGTTCACAGGCGTTGACAAAACAGGTAC
GCTTCCTGTACATAATTTGC

Downstream 100 bases:

>100_bases
TCCTGGCCCATAAACGGTTTCGAGAGGTAGCATCTTTGTTACGCAAGCTGAAGTCCATACTGCAGCAAATCGGTGTTGAC
GATCGGTTCGATACTGCCAC

Product: sigma-54 factor interaction domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MNEIKSLSAPDREFFRNVSQAAFTNPFSQSRIEVDRRIADAPPSMSWDDTVEQAVARVSERLRMLKLRGSTDLSGYSQSD
RELLRTAMLFDLYHQYRQPFDAFILKQLTAGDTPIPVPFASTILSALANIGLNSAAAQRFLAIFYQIRRAFYFIESGLTG
PSPAMQNLRLQLWNNIFTCNITWYEEYLWDRMEDFSTLLLGETGTGKGAAAAAIGRSGFIPFNERKGCFSESFTRSFIAI
NLSQYPESLLESELFGHKKGAFTGAIEHHEGVFDRCSPHGSIFLDEIGDVSIPVQIKLLQVLQERTFAPVGSHERRRFQG
RVIAATNRPLDEMRAKGQFRNDFYYRLCSDIVTVPSLRERIRKESRELEVLISRILVRMVGEKAREYTCLVYDIIRRDVG
SDYAWPGNVRELEQAVRRIIITRHYRGDRTPPNQENDDNNLLDAIRSESLDAESLLAGYCSVLYQRHGTYEEVARITNLD
RRTVKKYIRKD

Sequences:

>Translated_491_residues
MNEIKSLSAPDREFFRNVSQAAFTNPFSQSRIEVDRRIADAPPSMSWDDTVEQAVARVSERLRMLKLRGSTDLSGYSQSD
RELLRTAMLFDLYHQYRQPFDAFILKQLTAGDTPIPVPFASTILSALANIGLNSAAAQRFLAIFYQIRRAFYFIESGLTG
PSPAMQNLRLQLWNNIFTCNITWYEEYLWDRMEDFSTLLLGETGTGKGAAAAAIGRSGFIPFNERKGCFSESFTRSFIAI
NLSQYPESLLESELFGHKKGAFTGAIEHHEGVFDRCSPHGSIFLDEIGDVSIPVQIKLLQVLQERTFAPVGSHERRRFQG
RVIAATNRPLDEMRAKGQFRNDFYYRLCSDIVTVPSLRERIRKESRELEVLISRILVRMVGEKAREYTCLVYDIIRRDVG
SDYAWPGNVRELEQAVRRIIITRHYRGDRTPPNQENDDNNLLDAIRSESLDAESLLAGYCSVLYQRHGTYEEVARITNLD
RRTVKKYIRKD
>Mature_491_residues
MNEIKSLSAPDREFFRNVSQAAFTNPFSQSRIEVDRRIADAPPSMSWDDTVEQAVARVSERLRMLKLRGSTDLSGYSQSD
RELLRTAMLFDLYHQYRQPFDAFILKQLTAGDTPIPVPFASTILSALANIGLNSAAAQRFLAIFYQIRRAFYFIESGLTG
PSPAMQNLRLQLWNNIFTCNITWYEEYLWDRMEDFSTLLLGETGTGKGAAAAAIGRSGFIPFNERKGCFSESFTRSFIAI
NLSQYPESLLESELFGHKKGAFTGAIEHHEGVFDRCSPHGSIFLDEIGDVSIPVQIKLLQVLQERTFAPVGSHERRRFQG
RVIAATNRPLDEMRAKGQFRNDFYYRLCSDIVTVPSLRERIRKESRELEVLISRILVRMVGEKAREYTCLVYDIIRRDVG
SDYAWPGNVRELEQAVRRIIITRHYRGDRTPPNQENDDNNLLDAIRSESLDAESLLAGYCSVLYQRHGTYEEVARITNLD
RRTVKKYIRKD

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=228, Percent_Identity=40.3508771929825, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788550, Length=226, Percent_Identity=38.9380530973451, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI87082117, Length=262, Percent_Identity=37.4045801526718, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1790437, Length=344, Percent_Identity=34.0116279069767, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789087, Length=231, Percent_Identity=38.5281385281385, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789233, Length=291, Percent_Identity=32.3024054982818, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=39.3939393939394, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786524, Length=235, Percent_Identity=36.5957446808511, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1790299, Length=227, Percent_Identity=37.4449339207048, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI87081872, Length=238, Percent_Identity=34.453781512605, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787583, Length=223, Percent_Identity=35.4260089686099, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789828, Length=222, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 56267; Mature: 56267

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]