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The map label for this gene is hbaA [H]

Identifier: 118581760

GI number: 118581760

Start: 3708292

End: 3709809

Strand: Direct

Name: hbaA [H]

Synonym: Ppro_3360

Alternate gene names: 118581760

Gene position: 3708292-3709809 (Clockwise)

Preceding gene: 118581758

Following gene: 118581761

Centisome position: 92.52

GC content: 64.95

Gene sequence:

>1518_bases
TTGAGTACGCCAAATGCGGCAGTGGAGCTGATCAGGCAGAGCATTGAACGCCATCCCGATAAGGTGGCCTATTTCTGCGG
GGAGCGGGCGCTCAGCTATGCCGAGCTCAACCTGGCCAGCCGTCGCTTCGCCCTGCTCCTGCGGGAGCGGGGGATCGTCG
CTGGCGAGCGTGTCGTGGTGGCCCTTCCCGATTGTCTCTCCTTTCCGGTTGCCTTTCTGGGGTGCCTGCTGGCAGGGACG
GTGGCCGTGGCCGCCGGTTCAGCACTGGCGGATGACGACCTGGCCCACGTGATTGCCGACAGTGGCGCTCGCCTGCTGGT
CAGCCACGCCGAGCATGCCTCACGCCATGCCGCCCTGTGTCCCGGACTAGCCCAGGTCGTCTGTGACGATCTCGGCCCGA
CCCAGGAGGCTCCTTCATGCGTAGATGGCTTCCTCCCCTATCACCCCTCGGCCGAGGATTTCGCCTACATGCTCTACACG
TCCGGCTCCACCGGAAGGCCCAAGGGGATTCCCCATCGCCACACGAGCCTGCTTGTGCCCTGCGACCTGATGGGGCGGAG
CGTGCTGGGTATCACGGGCGATGACGTCATCTTCTCCACCAGCAAGCTCTCCTTCTGCTACGGCCTGCTCAACAGCCTCT
CGTTCGCGCTCCGTTTCGGGGCCACTGCCGTCCTCCTTCCGGGCAAGCCGGATGTCCTCTCCATTCTCCACGTCATAGAA
CAGCGCAGCCCCTCCATCTTCTTCTCCGTGCCGACCGTATTCCGGCAGATCATCCTCTCCTGCGCAGAACCAAAGCTGAA
ATTCCCCATGCGGCTCTGCTGCTCGGCCGGCGAGGCGCTCCCCCCGCTCCTGTTCCACGAGTGGCTACGGCTGACCGGCA
TGGAGATCATCGACGGCATCGGCGCTTCCGAACTCTCCCACCACTTCATCTGCAACCCGCCGGGGCGGGCGGTGGCCGGA
TCGGCGGGGATGGTGGTGCCCGGCTACCGGGTTCGCCTGGTGGACGACGCTGACAACGACGTTCCTTCCGGGAGCGAGGG
CAACCTGCTGGTGAGCGGCGAAACCGGAGCCCCCTTTTACTGGAACCTGCCCGAACTGAGCCGCACGACCATGCGGCCGG
ACGGCTTCATCCGCACCGGCGACATCTTCCTGGAGAAGGATGGATACTACTTCCATCGCGGCAGGAGCGACGACATGATC
AAGGTGGATGCCCAGTGGGTTTCACCGCTTGTGGTGGAGGATGTGCTACGTGGCCACCCCGCGGTGGCTGATTGCGCCGT
TGCCGCCGTGACTATCGGCGCCCTGGCCAGGCCGGGGGCCTTTATCGTACCCGCCGCCGGATTCGAGTCTTCTCCCGAGC
TGGTACGGGAGTTGAAGGAACTGGTCAGATCGCGCCTGCCGGATCATATGTGCCCGGCCCGCTTCCGTTTCATGACCAAC
CTGCCGCGCACCTCCACCGGAAAAGTCCGGCGCTGCTGCCTGAGGGAGCAGACACCCTCCCCGACCAAGCCGGAGTGA

Upstream 100 bases:

>100_bases
ATGCGATTGTCCCTTGTTGCGGCCGCGTAACCTATTGTATTGTGTTGTGCCGTGCCGTATGGCGCCGTGCGCGCAATCCA
CGAATCACCAGGGAGATGAC

Downstream 100 bases:

>100_bases
ACGTACACCTTACACAAAGGAGACCAAGATGGATCTGATTGACGAGATGAAAGAGATTCTGCTGAAAGTCGGGGTAGAGG
AGAGTGTTGTCAAGGAATTG

Product: benzoate-CoA ligase family

Products: NA

Alternate protein names: 4-hydroxybenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSTPNAAVELIRQSIERHPDKVAYFCGERALSYAELNLASRRFALLLRERGIVAGERVVVALPDCLSFPVAFLGCLLAGT
VAVAAGSALADDDLAHVIADSGARLLVSHAEHASRHAALCPGLAQVVCDDLGPTQEAPSCVDGFLPYHPSAEDFAYMLYT
SGSTGRPKGIPHRHTSLLVPCDLMGRSVLGITGDDVIFSTSKLSFCYGLLNSLSFALRFGATAVLLPGKPDVLSILHVIE
QRSPSIFFSVPTVFRQIILSCAEPKLKFPMRLCCSAGEALPPLLFHEWLRLTGMEIIDGIGASELSHHFICNPPGRAVAG
SAGMVVPGYRVRLVDDADNDVPSGSEGNLLVSGETGAPFYWNLPELSRTTMRPDGFIRTGDIFLEKDGYYFHRGRSDDMI
KVDAQWVSPLVVEDVLRGHPAVADCAVAAVTIGALARPGAFIVPAAGFESSPELVRELKELVRSRLPDHMCPARFRFMTN
LPRTSTGKVRRCCLREQTPSPTKPE

Sequences:

>Translated_505_residues
MSTPNAAVELIRQSIERHPDKVAYFCGERALSYAELNLASRRFALLLRERGIVAGERVVVALPDCLSFPVAFLGCLLAGT
VAVAAGSALADDDLAHVIADSGARLLVSHAEHASRHAALCPGLAQVVCDDLGPTQEAPSCVDGFLPYHPSAEDFAYMLYT
SGSTGRPKGIPHRHTSLLVPCDLMGRSVLGITGDDVIFSTSKLSFCYGLLNSLSFALRFGATAVLLPGKPDVLSILHVIE
QRSPSIFFSVPTVFRQIILSCAEPKLKFPMRLCCSAGEALPPLLFHEWLRLTGMEIIDGIGASELSHHFICNPPGRAVAG
SAGMVVPGYRVRLVDDADNDVPSGSEGNLLVSGETGAPFYWNLPELSRTTMRPDGFIRTGDIFLEKDGYYFHRGRSDDMI
KVDAQWVSPLVVEDVLRGHPAVADCAVAAVTIGALARPGAFIVPAAGFESSPELVRELKELVRSRLPDHMCPARFRFMTN
LPRTSTGKVRRCCLREQTPSPTKPE
>Mature_504_residues
STPNAAVELIRQSIERHPDKVAYFCGERALSYAELNLASRRFALLLRERGIVAGERVVVALPDCLSFPVAFLGCLLAGTV
AVAAGSALADDDLAHVIADSGARLLVSHAEHASRHAALCPGLAQVVCDDLGPTQEAPSCVDGFLPYHPSAEDFAYMLYTS
GSTGRPKGIPHRHTSLLVPCDLMGRSVLGITGDDVIFSTSKLSFCYGLLNSLSFALRFGATAVLLPGKPDVLSILHVIEQ
RSPSIFFSVPTVFRQIILSCAEPKLKFPMRLCCSAGEALPPLLFHEWLRLTGMEIIDGIGASELSHHFICNPPGRAVAGS
AGMVVPGYRVRLVDDADNDVPSGSEGNLLVSGETGAPFYWNLPELSRTTMRPDGFIRTGDIFLEKDGYYFHRGRSDDMIK
VDAQWVSPLVVEDVLRGHPAVADCAVAAVTIGALARPGAFIVPAAGFESSPELVRELKELVRSRLPDHMCPARFRFMTNL
PRTSTGKVRRCCLREQTPSPTKPE

Specific function: Catalyzes the ligation of 4-hydroxybenzoate, benzoate or cyclohex-1,4-dienecarboxylate and CoA at the expense of ATP. The enzyme shows low activity towards cyclo-2,5-dienecarboxylate, 4- fluorobenzoate, 4-chlorobenzoate and 2-methoxybenzoate [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=494, Percent_Identity=27.5303643724696, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI38505220, Length=501, Percent_Identity=27.9441117764471, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI122937307, Length=502, Percent_Identity=27.4900398406374, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI58082049, Length=499, Percent_Identity=27.2545090180361, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI157311624, Length=499, Percent_Identity=27.0541082164329, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI157311622, Length=499, Percent_Identity=27.0541082164329, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI187761345, Length=533, Percent_Identity=28.8930581613508, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI187761343, Length=533, Percent_Identity=28.8930581613508, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI115511026, Length=497, Percent_Identity=27.1629778672032, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI8923896, Length=574, Percent_Identity=24.7386759581882, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI28416953, Length=561, Percent_Identity=25.3119429590018, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI253970489, Length=366, Percent_Identity=27.3224043715847, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI42544134, Length=321, Percent_Identity=26.4797507788162, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI156151445, Length=516, Percent_Identity=23.062015503876, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI195947378, Length=414, Percent_Identity=21.9806763285024, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1788107, Length=534, Percent_Identity=26.9662921348315, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1790505, Length=545, Percent_Identity=25.1376146788991, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI145693145, Length=522, Percent_Identity=24.904214559387, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1786801, Length=520, Percent_Identity=28.4615384615385, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI221142682, Length=504, Percent_Identity=23.2142857142857, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786810, Length=515, Percent_Identity=22.7184466019417, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789201, Length=351, Percent_Identity=27.3504273504274, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI32563687, Length=498, Percent_Identity=25.7028112449799, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17558820, Length=506, Percent_Identity=25.098814229249, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17531443, Length=519, Percent_Identity=24.2774566473988, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=506, Percent_Identity=25.296442687747, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=509, Percent_Identity=24.75442043222, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI71982997, Length=562, Percent_Identity=23.8434163701068, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71983001, Length=562, Percent_Identity=23.8434163701068, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71994694, Length=539, Percent_Identity=21.7068645640074, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71994703, Length=539, Percent_Identity=21.7068645640074, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71994690, Length=539, Percent_Identity=21.7068645640074, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=530, Percent_Identity=23.5849056603774, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=361, Percent_Identity=23.5457063711911, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323182, Length=559, Percent_Identity=23.613595706619, Blast_Score=109, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6319699, Length=354, Percent_Identity=26.5536723163842, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319264, Length=558, Percent_Identity=22.4014336917563, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24667955, Length=546, Percent_Identity=27.6556776556777, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI62472339, Length=545, Percent_Identity=27.3394495412844, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24667959, Length=501, Percent_Identity=26.9461077844311, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI18859661, Length=539, Percent_Identity=25.0463821892393, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21355225, Length=541, Percent_Identity=25.1386321626617, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648260, Length=516, Percent_Identity=24.2248062015504, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24581924, Length=516, Percent_Identity=22.6744186046512, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356947, Length=475, Percent_Identity=22.3157894736842, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24656500, Length=372, Percent_Identity=23.6559139784946, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25; =6.2.1.27 [H]

Molecular weight: Translated: 54546; Mature: 54415

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPNAAVELIRQSIERHPDKVAYFCGERALSYAELNLASRRFALLLRERGIVAGERVVV
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
ALPDCLSFPVAFLGCLLAGTVAVAAGSALADDDLAHVIADSGARLLVSHAEHASRHAALC
ECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHC
PGLAQVVCDDLGPTQEAPSCVDGFLPYHPSAEDFAYMLYTSGSTGRPKGIPHRHTSLLVP
CHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEE
CDLMGRSVLGITGDDVIFSTSKLSFCYGLLNSLSFALRFGATAVLLPGKPDVLSILHVIE
HHHHCCEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHH
QRSPSIFFSVPTVFRQIILSCAEPKLKFPMRLCCSAGEALPPLLFHEWLRLTGMEIIDGI
CCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
GASELSHHFICNPPGRAVAGSAGMVVPGYRVRLVDDADNDVPSGSEGNLLVSGETGAPFY
CHHHHCCCEEECCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCEEEECCCCCCEE
WNLPELSRTTMRPDGFIRTGDIFLEKDGYYFHRGRSDDMIKVDAQWVSPLVVEDVLRGHP
ECCCHHHHHCCCCCCCEEECCEEEECCCEEEECCCCCCEEEECHHHHCHHHHHHHHCCCC
AVADCAVAAVTIGALARPGAFIVPAAGFESSPELVRELKELVRSRLPDHMCPARFRFMTN
CHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
LPRTSTGKVRRCCLREQTPSPTKPE
CCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
STPNAAVELIRQSIERHPDKVAYFCGERALSYAELNLASRRFALLLRERGIVAGERVVV
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
ALPDCLSFPVAFLGCLLAGTVAVAAGSALADDDLAHVIADSGARLLVSHAEHASRHAALC
ECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHC
PGLAQVVCDDLGPTQEAPSCVDGFLPYHPSAEDFAYMLYTSGSTGRPKGIPHRHTSLLVP
CHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEE
CDLMGRSVLGITGDDVIFSTSKLSFCYGLLNSLSFALRFGATAVLLPGKPDVLSILHVIE
HHHHCCEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHH
QRSPSIFFSVPTVFRQIILSCAEPKLKFPMRLCCSAGEALPPLLFHEWLRLTGMEIIDGI
CCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
GASELSHHFICNPPGRAVAGSAGMVVPGYRVRLVDDADNDVPSGSEGNLLVSGETGAPFY
CHHHHCCCEEECCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCEEEECCCCCCEE
WNLPELSRTTMRPDGFIRTGDIFLEKDGYYFHRGRSDDMIKVDAQWVSPLVVEDVLRGHP
ECCCHHHHHCCCCCCCEEECCEEEECCCEEEECCCCCCEEEECHHHHCHHHHHHHHCCCC
AVADCAVAAVTIGALARPGAFIVPAAGFESSPELVRELKELVRSRLPDHMCPARFRFMTN
CHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
LPRTSTGKVRRCCLREQTPSPTKPE
CCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8300518 [H]