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The map label for this gene is tyrS [H]

Identifier: 118581710

GI number: 118581710

Start: 3648084

End: 3649298

Strand: Reverse

Name: tyrS [H]

Synonym: Ppro_3310

Alternate gene names: 118581710

Gene position: 3649298-3648084 (Counterclockwise)

Preceding gene: 118581711

Following gene: 118581709

Centisome position: 91.05

GC content: 62.14

Gene sequence:

>1215_bases
ATGTCCGTAGCCGAACAGATGGCCGTTATCAAGCGTGGCGCCGTGGAGATCCTGATCGAGAAGGAGCTTGAGGCGAAACT
GGAGAAGTCGCTGAAAACCGGCGTGCCGCTCAGGATCAAGGCCGGCTTCGATCCCACCGCCCCTGACCTGCATCTGGGAC
ACACCGTGCTGATCCACAAGCTGCGCCAGTTTCAGCAGCTGGGTCACGAGGTGAACTTCCTGATCGGCGATTTCACCGGC
ATGATCGGCGACCCCACCGGCAAATCGGAGACCCGCAAGGTCCTGACCCGCGAGGATGTGCTCAAGAACGCCGAGACCTA
CAAGGAGCAGGTCTTCAAGATCCTGGATCCGGACAAGACCAAGGTGGTGTTCAACTCCAGCTGGCTGAACGACCTGGGCT
GCGGCGGTCTGATCGCCCTGGCATCCAAGTACACCGTGGCCCGCATGCTGGAGCGGGACGACTTCCACAAACGCTTCTCC
AGCCAGCAGCCGATCAGCATCCATGAGTTCCTCTACCCGCTGGTGCAGGGGTACGACTCGGTTGCCCTGAAATCGGACGT
GGAGCTGGGGGGGACGGACCAGAAGTTCAACCTGCTGGTGGGGCGCGAGTTGCAGCGCGAGTGGGGCCAGGAGCCCCAGT
GCGTGCTGACTATGCCGCTCCTGGAGGGGCTGGACGGGGTCAACAAGATGTCCAAGTCACTGGGCAACTACATCGGCATC
AATGAACCGGGCGACGAGATCTTCGGCAAGGTCATGTCAATCTCCGACGAGCTGATGGTCCGTTACTACGAACTGCTCTC
CGACATGCCGCTCCCGGAGCTTGAGAAGCTCAAACAGGGGCTGGCGGACAACTCCCTGCACCCCATGGAAACCAAGAAGG
CCCTGGGCCGTGAGCTGGTGGGACGCTTCCACGGAGCAGCGGCGGCCAGGGATGCGGAGGAAAATTTCGTCAAACGCTTC
CGCGACAACCAGATCCCGGACGACATGCCCCAGATCAGTCACTGTCTGGCCGACGGCACCCTGCTTTTGGCCCGCGCCAT
GAGCGAGGCGGGGCTGACCAAGTCCAACGGCGAGGCGCGGCGTTCCATCGATCAGGGGGGGGTCAAGCTGAACGGCGAGA
AGATCTCCAACCCCAACCTGGAGCTGGATGCGGCCGGCGAGTACATCGTCCAGATCGGGCGCCGCCGTTTCGGCCGGATC
ATCGTCAGCGCATGA

Upstream 100 bases:

>100_bases
GGTCTGCTGCTGAAAAACAACGCCACAGCGGTCACGGAATTATGGACTGAATCCATGATGCCGTGACTGCTGTGGCTACA
TTATTATGAGGAGCCTGAAC

Downstream 100 bases:

>100_bases
TCCATCTGACCAATATCAGCAAGCAGTACGGCTCCCAGGTTCTCTTCCGCGAGGCGGGTTTCCAGATCCTGCCCGCAAGC
CGCAGCGGCCTGGTGGGCCC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSVAEQMAVIKRGAVEILIEKELEAKLEKSLKTGVPLRIKAGFDPTAPDLHLGHTVLIHKLRQFQQLGHEVNFLIGDFTG
MIGDPTGKSETRKVLTREDVLKNAETYKEQVFKILDPDKTKVVFNSSWLNDLGCGGLIALASKYTVARMLERDDFHKRFS
SQQPISIHEFLYPLVQGYDSVALKSDVELGGTDQKFNLLVGRELQREWGQEPQCVLTMPLLEGLDGVNKMSKSLGNYIGI
NEPGDEIFGKVMSISDELMVRYYELLSDMPLPELEKLKQGLADNSLHPMETKKALGRELVGRFHGAAAARDAEENFVKRF
RDNQIPDDMPQISHCLADGTLLLARAMSEAGLTKSNGEARRSIDQGGVKLNGEKISNPNLELDAAGEYIVQIGRRRFGRI
IVSA

Sequences:

>Translated_404_residues
MSVAEQMAVIKRGAVEILIEKELEAKLEKSLKTGVPLRIKAGFDPTAPDLHLGHTVLIHKLRQFQQLGHEVNFLIGDFTG
MIGDPTGKSETRKVLTREDVLKNAETYKEQVFKILDPDKTKVVFNSSWLNDLGCGGLIALASKYTVARMLERDDFHKRFS
SQQPISIHEFLYPLVQGYDSVALKSDVELGGTDQKFNLLVGRELQREWGQEPQCVLTMPLLEGLDGVNKMSKSLGNYIGI
NEPGDEIFGKVMSISDELMVRYYELLSDMPLPELEKLKQGLADNSLHPMETKKALGRELVGRFHGAAAARDAEENFVKRF
RDNQIPDDMPQISHCLADGTLLLARAMSEAGLTKSNGEARRSIDQGGVKLNGEKISNPNLELDAAGEYIVQIGRRRFGRI
IVSA
>Mature_403_residues
SVAEQMAVIKRGAVEILIEKELEAKLEKSLKTGVPLRIKAGFDPTAPDLHLGHTVLIHKLRQFQQLGHEVNFLIGDFTGM
IGDPTGKSETRKVLTREDVLKNAETYKEQVFKILDPDKTKVVFNSSWLNDLGCGGLIALASKYTVARMLERDDFHKRFSS
QQPISIHEFLYPLVQGYDSVALKSDVELGGTDQKFNLLVGRELQREWGQEPQCVLTMPLLEGLDGVNKMSKSLGNYIGIN
EPGDEIFGKVMSISDELMVRYYELLSDMPLPELEKLKQGLADNSLHPMETKKALGRELVGRFHGAAAARDAEENFVKRFR
DNQIPDDMPQISHCLADGTLLLARAMSEAGLTKSNGEARRSIDQGGVKLNGEKISNPNLELDAAGEYIVQIGRRRFGRII
VSA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=410, Percent_Identity=27.5609756097561, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1787925, Length=416, Percent_Identity=27.8846153846154, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25145802, Length=263, Percent_Identity=26.9961977186312, Blast_Score=88, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6325160, Length=417, Percent_Identity=24.220623501199, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI20130383, Length=406, Percent_Identity=25.3694581280788, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44960; Mature: 44829

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAEQMAVIKRGAVEILIEKELEAKLEKSLKTGVPLRIKAGFDPTAPDLHLGHTVLIHK
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHH
LRQFQQLGHEVNFLIGDFTGMIGDPTGKSETRKVLTREDVLKNAETYKEQVFKILDPDKT
HHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KVVFNSSWLNDLGCGGLIALASKYTVARMLERDDFHKRFSSQQPISIHEFLYPLVQGYDS
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
VALKSDVELGGTDQKFNLLVGRELQREWGQEPQCVLTMPLLEGLDGVNKMSKSLGNYIGI
HHECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCHHHHHHHHHCCCCCC
NEPGDEIFGKVMSISDELMVRYYELLSDMPLPELEKLKQGLADNSLHPMETKKALGRELV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHH
GRFHGAAAARDAEENFVKRFRDNQIPDDMPQISHCLADGTLLLARAMSEAGLTKSNGEAR
HHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHH
RSIDQGGVKLNGEKISNPNLELDAAGEYIVQIGRRRFGRIIVSA
HHHHHCCEEECCCCCCCCCEEEECCHHHHHHHHHHHHCEEEECC
>Mature Secondary Structure 
SVAEQMAVIKRGAVEILIEKELEAKLEKSLKTGVPLRIKAGFDPTAPDLHLGHTVLIHK
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHH
LRQFQQLGHEVNFLIGDFTGMIGDPTGKSETRKVLTREDVLKNAETYKEQVFKILDPDKT
HHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KVVFNSSWLNDLGCGGLIALASKYTVARMLERDDFHKRFSSQQPISIHEFLYPLVQGYDS
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
VALKSDVELGGTDQKFNLLVGRELQREWGQEPQCVLTMPLLEGLDGVNKMSKSLGNYIGI
HHECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCHHHHHHHHHCCCCCC
NEPGDEIFGKVMSISDELMVRYYELLSDMPLPELEKLKQGLADNSLHPMETKKALGRELV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHH
GRFHGAAAARDAEENFVKRFRDNQIPDDMPQISHCLADGTLLLARAMSEAGLTKSNGEAR
HHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHH
RSIDQGGVKLNGEKISNPNLELDAAGEYIVQIGRRRFGRIIVSA
HHHHHCCEEECCCCCCCCCEEEECCHHHHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA