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The map label for this gene is pksE [H]

Identifier: 118581501

GI number: 118581501

Start: 3388234

End: 3389898

Strand: Direct

Name: pksE [H]

Synonym: Ppro_3100

Alternate gene names: 118581501

Gene position: 3388234-3389898 (Clockwise)

Preceding gene: 118581500

Following gene: 118581502

Centisome position: 84.54

GC content: 65.95

Gene sequence:

>1665_bases
ATGTCGTATCAGTCACCGGGTACCACCACAATGGAACACCCCCTTCTGGGGTGGTGTCGGCCAGACGCCGCCCCCTGCTC
CGGAACAGACCTGGCCGATGGCCTTCGCCGCCTTCACCGGCCGCTCTTCATCGCCGCCCAGGACGATTCCCTGACCCCCC
TCTCCAGGCCACGGCTGCTGGCGGACAGTGAGCCACCACCGAGCGGCGCCCTGCCGCTGGTCGGCTTCTCCCCCCCATGT
CCCCCTGAAAACCTGGGCGATCCCTCCTTCTGCGGGGAACTGGGAATCCGCTACCCCTACCTAGGCGGTTCCATGGCCAA
GGGGATCAGTTCGGTGGCCATGGCGCTGGCCCTGGGCAGGGCAGGCATGCTCGGCTTCTTCGGCGCCGCGGGCCTGACCA
TCTCCCAGGTGGAAGAGGCCGTCAACCAGCTCACGTCCAGCAAGGTTCCCTTCGGCTGCAACCTGATCCATTCGCCCCAC
GACCCGGAACTGGAGCAGACCCTGGTTCAGCTCTACCTGGAGCGGAACGTGCGGCTGATGGAGGCATCGGCCTTCATGAA
GCTGACCCTGCCGCTGGTGCGCTACCGCCTGGCCGGCATCCGCCGTAATGACGACGGCAGCATCCACACCCCCAACCGGA
TCATCGCCAAGGTCTCCCGAGAGGAGTTGGCGGCACGGTTCTTCGCCCCGCCACCCGAGGCGTTTCTTCACGAGCTGGTG
GCGGACAAAGAGATCACCCACGAACAGGCGGAACTGGCTGCCCATATCCCCATGGCCCAGGAGATCACCGCCGAGGCCGA
TTCCGGCGGGCACACCGACAACCGGCCAGCCAACGCCCTCTTCCCCACCATCGCATCCCTGGCGGCCCGCATGCAGGCCG
AGCACAACTATGACCTGAGGCTGCGCGTCGGCCTGGGGGGAGGCATCTCCACACCTGCCGCGGCTGCGGCGGCCTTTGTC
ATGGGCGCGGCCTACGTCATGACCGGCTCGGTCAACCAGGCATGCCGGGAGTCGGGAACCTCGGACGAGGTGCGCGCCAT
GCTGGCCGAAACCCGCCAGGCAGACGTGACCATGGCCCCCTCGGCCGACATGTTCGAGATGGGGGTCAACGTGCAGGTAC
TCAAGCGCGGCACCATGTTCCCCATGCGCGCCCAGAAGCTGTACGAGATCTACCGCGCCTGGCCGAGCCTGGAGCAGATC
CCGACTCTCGAGCGTGAAAAGCTGGAAAAGACCATCTTCCAGGCGCCGCTGGAGCAGATCTGGAAGATCACCCGCGACTA
CTTTCTTAAGCGCGCGCCGCAGCAGGTCAGCCGCGCCGAGCAGGACCCCAAACACCGCATGGCACTGGTCTTCCGCTGGT
ACCTGGGACAGGCGGCCCACTGGGCCAAGGATGGCGACAGATCGCGCAGGATCGACTACCAGATCTGGTGCGGCCCGGCC
ATGGGCGCCTTCAACGAATGGGTCGCCGACTCCTTCCTGGAGCCCCCGGCCAACCGCGACGTGGTAACGGTCGGCCTGAA
CATCCTCTTCGGCGCAGCGCTCATGACCCGCGCCAGCGTACTGCGCTGCCAGGGGCTCCCCATCCCCCGGGAAGCCCTGA
CCACGAAACCGCTCGAACTCGCACATATCAAGGAGTATCTATCAGGTGAAAACGCTCGATCCTAA

Upstream 100 bases:

>100_bases
AACGACAAGGAAACAGCGGACCGGGCACATCGGTCACCCCCCAAAAGCCGCCGTATCCGTTGCAGTTCATTGCACCATTC
AAAGAGGAGACTCCCGTTCG

Downstream 100 bases:

>100_bases
ACCGTCATCCTCCACCAACTCGGTACCACTGGCCATCATCGGCATCGGCTGTCTCTTCCCCCAGGCGGATGACATGCACG
CCTACTGGTCCAACATAACG

Product: 2-nitropropane dioxygenase, NPD

Products: NA

Alternate protein names: Malonyl CoA-acyl carrier protein transacylase; MCT [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSYQSPGTTTMEHPLLGWCRPDAAPCSGTDLADGLRRLHRPLFIAAQDDSLTPLSRPRLLADSEPPPSGALPLVGFSPPC
PPENLGDPSFCGELGIRYPYLGGSMAKGISSVAMALALGRAGMLGFFGAAGLTISQVEEAVNQLTSSKVPFGCNLIHSPH
DPELEQTLVQLYLERNVRLMEASAFMKLTLPLVRYRLAGIRRNDDGSIHTPNRIIAKVSREELAARFFAPPPEAFLHELV
ADKEITHEQAELAAHIPMAQEITAEADSGGHTDNRPANALFPTIASLAARMQAEHNYDLRLRVGLGGGISTPAAAAAAFV
MGAAYVMTGSVNQACRESGTSDEVRAMLAETRQADVTMAPSADMFEMGVNVQVLKRGTMFPMRAQKLYEIYRAWPSLEQI
PTLEREKLEKTIFQAPLEQIWKITRDYFLKRAPQQVSRAEQDPKHRMALVFRWYLGQAAHWAKDGDRSRRIDYQIWCGPA
MGAFNEWVADSFLEPPANRDVVTVGLNILFGAALMTRASVLRCQGLPIPREALTTKPLELAHIKEYLSGENARS

Sequences:

>Translated_554_residues
MSYQSPGTTTMEHPLLGWCRPDAAPCSGTDLADGLRRLHRPLFIAAQDDSLTPLSRPRLLADSEPPPSGALPLVGFSPPC
PPENLGDPSFCGELGIRYPYLGGSMAKGISSVAMALALGRAGMLGFFGAAGLTISQVEEAVNQLTSSKVPFGCNLIHSPH
DPELEQTLVQLYLERNVRLMEASAFMKLTLPLVRYRLAGIRRNDDGSIHTPNRIIAKVSREELAARFFAPPPEAFLHELV
ADKEITHEQAELAAHIPMAQEITAEADSGGHTDNRPANALFPTIASLAARMQAEHNYDLRLRVGLGGGISTPAAAAAAFV
MGAAYVMTGSVNQACRESGTSDEVRAMLAETRQADVTMAPSADMFEMGVNVQVLKRGTMFPMRAQKLYEIYRAWPSLEQI
PTLEREKLEKTIFQAPLEQIWKITRDYFLKRAPQQVSRAEQDPKHRMALVFRWYLGQAAHWAKDGDRSRRIDYQIWCGPA
MGAFNEWVADSFLEPPANRDVVTVGLNILFGAALMTRASVLRCQGLPIPREALTTKPLELAHIKEYLSGENARS
>Mature_553_residues
SYQSPGTTTMEHPLLGWCRPDAAPCSGTDLADGLRRLHRPLFIAAQDDSLTPLSRPRLLADSEPPPSGALPLVGFSPPCP
PENLGDPSFCGELGIRYPYLGGSMAKGISSVAMALALGRAGMLGFFGAAGLTISQVEEAVNQLTSSKVPFGCNLIHSPHD
PELEQTLVQLYLERNVRLMEASAFMKLTLPLVRYRLAGIRRNDDGSIHTPNRIIAKVSREELAARFFAPPPEAFLHELVA
DKEITHEQAELAAHIPMAQEITAEADSGGHTDNRPANALFPTIASLAARMQAEHNYDLRLRVGLGGGISTPAAAAAAFVM
GAAYVMTGSVNQACRESGTSDEVRAMLAETRQADVTMAPSADMFEMGVNVQVLKRGTMFPMRAQKLYEIYRAWPSLEQIP
TLEREKLEKTIFQAPLEQIWKITRDYFLKRAPQQVSRAEQDPKHRMALVFRWYLGQAAHWAKDGDRSRRIDYQIWCGPAM
GAFNEWVADSFLEPPANRDVVTVGLNILFGAALMTRASVLRCQGLPIPREALTTKPLELAHIKEYLSGENARS

Specific function: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductas

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the fabD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR001227
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013785
- InterPro:   IPR004410
- InterPro:   IPR016036
- InterPro:   IPR014179 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF03060 NPD [H]

EC number: =2.3.1.39 [H]

Molecular weight: Translated: 60796; Mature: 60665

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQSPGTTTMEHPLLGWCRPDAAPCSGTDLADGLRRLHRPLFIAAQDDSLTPLSRPRLL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCC
ADSEPPPSGALPLVGFSPPCPPENLGDPSFCGELGIRYPYLGGSMAKGISSVAMALALGR
CCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
AGMLGFFGAAGLTISQVEEAVNQLTSSKVPFGCNLIHSPHDPELEQTLVQLYLERNVRLM
CHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCEEE
EASAFMKLTLPLVRYRLAGIRRNDDGSIHTPNRIIAKVSREELAARFFAPPPEAFLHELV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ADKEITHEQAELAAHIPMAQEITAEADSGGHTDNRPANALFPTIASLAARMQAEHNYDLR
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
LRVGLGGGISTPAAAAAAFVMGAAYVMTGSVNQACRESGTSDEVRAMLAETRQADVTMAP
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCEEECC
SADMFEMGVNVQVLKRGTMFPMRAQKLYEIYRAWPSLEQIPTLEREKLEKTIFQAPLEQI
CCCHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHH
WKITRDYFLKRAPQQVSRAEQDPKHRMALVFRWYLGQAAHWAKDGDRSRRIDYQIWCGPA
HHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEEEECCC
MGAFNEWVADSFLEPPANRDVVTVGLNILFGAALMTRASVLRCQGLPIPREALTTKPLEL
HHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHH
AHIKEYLSGENARS
HHHHHHHCCCCCCC
>Mature Secondary Structure 
SYQSPGTTTMEHPLLGWCRPDAAPCSGTDLADGLRRLHRPLFIAAQDDSLTPLSRPRLL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCC
ADSEPPPSGALPLVGFSPPCPPENLGDPSFCGELGIRYPYLGGSMAKGISSVAMALALGR
CCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
AGMLGFFGAAGLTISQVEEAVNQLTSSKVPFGCNLIHSPHDPELEQTLVQLYLERNVRLM
CHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCEEE
EASAFMKLTLPLVRYRLAGIRRNDDGSIHTPNRIIAKVSREELAARFFAPPPEAFLHELV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ADKEITHEQAELAAHIPMAQEITAEADSGGHTDNRPANALFPTIASLAARMQAEHNYDLR
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
LRVGLGGGISTPAAAAAAFVMGAAYVMTGSVNQACRESGTSDEVRAMLAETRQADVTMAP
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCEEECC
SADMFEMGVNVQVLKRGTMFPMRAQKLYEIYRAWPSLEQIPTLEREKLEKTIFQAPLEQI
CCCHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHH
WKITRDYFLKRAPQQVSRAEQDPKHRMALVFRWYLGQAAHWAKDGDRSRRIDYQIWCGPA
HHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEEEECCC
MGAFNEWVADSFLEPPANRDVVTVGLNILFGAALMTRASVLRCQGLPIPREALTTKPLEL
HHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHH
AHIKEYLSGENARS
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]