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The map label for this gene is glyS [H]

Identifier: 118581462

GI number: 118581462

Start: 3345538

End: 3347601

Strand: Reverse

Name: glyS [H]

Synonym: Ppro_3059

Alternate gene names: 118581462

Gene position: 3347601-3345538 (Counterclockwise)

Preceding gene: 118581463

Following gene: 118581461

Centisome position: 83.52

GC content: 62.55

Gene sequence:

>2064_bases
ATGGGCGTCAAAGAACTCTTTCTGGAAATCGGTACCGAGGAGATTCCGGCCGGGTTTATTCCCCGGGCCATGGCCGAGAT
GGAGGCCATGATCACCAGGGAACTGACCAATGCCCGCCTGAGCTATTCCACGGTGCAGACCATGGCAACGCCCCGCCGCT
TGGCGCTGGTGGTGAAGGATATCCCGGCGGTGCAGCCGGATGCCGAGATTACCGCCACCGGCCCCTCCAAAAAGGCCGCC
TTCGATGCCGACGGCAAACCGACCAAAGCAGCGGAAGGTTTCGCCAGGGGGCAGGGGGTAACGGTCGACCAGCTTCAGAT
CGTCACCACCGACAAGGGAGAGTATCTGGCGGTGACCAAGACGGAAACCGGACGGCCCACCCCTGAGCTGCTGTCGGAGA
TCCTGCCGGCGATGATCGCCAGCATCCCCTTCAAGAAGTCCATGCGCTGGGGGGATCTGGATGTGCGCTTTGCCAGGCCG
ATCCACTGGATCGTGGCCCTGTTCGACGGGATCGTGGTTCCCTTCGTTTTCGGCAACATCGAGAGCGGTAGTGTCTCCCG
CGGGCACCGCTTCATGGCCAATACCTCATTCCCGGTGCGCAATTTCAGCCACTACCTGGACGAATGCGAGCGCCACTTCG
TGATTGTTGACCCGGAACGGCGCAAGGAGCTGATCCGTCGCGAGAGCCACCGTGTGGCCGCGGCAGCCGGCGGACACCTG
CTGCCCGACGAGGAGCTGCTGGAGCAGATCACCTACCTGGTGGAGTATCCCAGCGCCGTGCACGGAACCTTCTCGCGTGA
ATTCCTCAAGGTCCCCAAAGAGGTGCTGATCACCTCCATGCGCAGCCACCAGCGCTACTTCTCCATCGTGGATGGCCAGG
GCAACCTCATGCCCGGCTTCATCACCATCAACAACACTCTCACCGATGATCCGGGTGTGGTGGTCAAGGGGAACGAGCGT
GTCCTCCGGGCGCGTCTCTCCGACGCACGCTTCTTCTTCGAAGAGGACCAGAAGGTCAAGCTGGATGAGCGTGTGGAAGA
GTTGAAAAAGGTGGTCTACCAGCAGAAGCTGGGCACCTCCTTCGAAAAGATGGAACGCTTCAGGGCGCTGGCCGAAGAAC
TGGCCGACAGGCTGAACCCGGCTGTGAAATCAGAGACATCCCGTGCCGCCTGGCTCTGCAAGGCGGATCTGGTGTCCGGC
ATGGTCGGTGAGTTTCCCGAGGTGCAGGGGATCATGGGGCGTGAATATGCGCTGCTGGAAGGTGAATCAGGGGACGTGGC
CTGTGCCATTGCCGAGCACTACCTGCCGATCCAGGCCGGTGGGGAGCTGCCTGCCAGCGACATCGGTGCGTTCGTCTCCA
TTGCTGACAAGCTTGACACCATCTGCGGCTGTTTCAGCGTGGGGCTGATTCCCACCGGCGCCGCCGACCCCTATGCCCTG
CGGCGGGCCACCATCGGCATCATCGCCATCATCCTGGACAGGGGCTATCGCCTGGGGCTTGCCTCCCTGATCGACCGCTC
CCTTGATCTGCTGGAGGCCAAGCTCCTCAGGCCGAAGGAGCAGGTTGCAACTGAGGTGCTGGAGTTTTTCCGCGGCCGCT
TCGTCAACCTCCTGGGAGGCGAATTCGACGCTGACGCGGTGGAGGCGGCTGTTTCGGCCGAGTTTGACGACCTGGTGGAT
GTGAAGAGCCGCGTCGCGGCCCTGGCGCAGTTCAAGTCCCACCCCGACTTCGAGCCGCTGGCGGTGGCCTTCAAGCGCAT
TGGCAACATCATCAAGGGGGGCACCGATGCCTTGGTCGATCCGTCCCTGTTCCAGGACGCCGCCGAGGGTGCCCTGTACC
AGGCCTTCCATGAGGTCAAGGTGCATGTCAGCGCACTGGTCGAGCAGGGAGCGTGGCAGGATGCCCTGACCCGTATCGCC
GGTCTGCGCGCCCCGGTTGACGGCTTTTTCGACAAGGTCATGGTCATGGCCGAGGATCAGCGGGTGCGCAGCAACCGCCT
GGCCCTGCTGACGGCCATAGACCGCATGTTCGGACGGATCGCCGATTTTTCACGCATAGCGTAA

Upstream 100 bases:

>100_bases
ACATCGGACGGGTCAGGAACGTGGCCCGCGTCTGCGCCGAGGGATACCTGCGCATGCGCGAACGGCTCGGCTTTCCACTG
CTGAAAGGAGGTGTTGCCTG

Downstream 100 bases:

>100_bases
CAACCGGATCAGTATCCGGGCGTTATCCTGCGCCGCCGCTTTTTGAGTTGCACCCTGTCTCCAAATCGTCTTCAGGGAGA
GTGCCGACATGTCCGCTACC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MGVKELFLEIGTEEIPAGFIPRAMAEMEAMITRELTNARLSYSTVQTMATPRRLALVVKDIPAVQPDAEITATGPSKKAA
FDADGKPTKAAEGFARGQGVTVDQLQIVTTDKGEYLAVTKTETGRPTPELLSEILPAMIASIPFKKSMRWGDLDVRFARP
IHWIVALFDGIVVPFVFGNIESGSVSRGHRFMANTSFPVRNFSHYLDECERHFVIVDPERRKELIRRESHRVAAAAGGHL
LPDEELLEQITYLVEYPSAVHGTFSREFLKVPKEVLITSMRSHQRYFSIVDGQGNLMPGFITINNTLTDDPGVVVKGNER
VLRARLSDARFFFEEDQKVKLDERVEELKKVVYQQKLGTSFEKMERFRALAEELADRLNPAVKSETSRAAWLCKADLVSG
MVGEFPEVQGIMGREYALLEGESGDVACAIAEHYLPIQAGGELPASDIGAFVSIADKLDTICGCFSVGLIPTGAADPYAL
RRATIGIIAIILDRGYRLGLASLIDRSLDLLEAKLLRPKEQVATEVLEFFRGRFVNLLGGEFDADAVEAAVSAEFDDLVD
VKSRVAALAQFKSHPDFEPLAVAFKRIGNIIKGGTDALVDPSLFQDAAEGALYQAFHEVKVHVSALVEQGAWQDALTRIA
GLRAPVDGFFDKVMVMAEDQRVRSNRLALLTAIDRMFGRIADFSRIA

Sequences:

>Translated_687_residues
MGVKELFLEIGTEEIPAGFIPRAMAEMEAMITRELTNARLSYSTVQTMATPRRLALVVKDIPAVQPDAEITATGPSKKAA
FDADGKPTKAAEGFARGQGVTVDQLQIVTTDKGEYLAVTKTETGRPTPELLSEILPAMIASIPFKKSMRWGDLDVRFARP
IHWIVALFDGIVVPFVFGNIESGSVSRGHRFMANTSFPVRNFSHYLDECERHFVIVDPERRKELIRRESHRVAAAAGGHL
LPDEELLEQITYLVEYPSAVHGTFSREFLKVPKEVLITSMRSHQRYFSIVDGQGNLMPGFITINNTLTDDPGVVVKGNER
VLRARLSDARFFFEEDQKVKLDERVEELKKVVYQQKLGTSFEKMERFRALAEELADRLNPAVKSETSRAAWLCKADLVSG
MVGEFPEVQGIMGREYALLEGESGDVACAIAEHYLPIQAGGELPASDIGAFVSIADKLDTICGCFSVGLIPTGAADPYAL
RRATIGIIAIILDRGYRLGLASLIDRSLDLLEAKLLRPKEQVATEVLEFFRGRFVNLLGGEFDADAVEAAVSAEFDDLVD
VKSRVAALAQFKSHPDFEPLAVAFKRIGNIIKGGTDALVDPSLFQDAAEGALYQAFHEVKVHVSALVEQGAWQDALTRIA
GLRAPVDGFFDKVMVMAEDQRVRSNRLALLTAIDRMFGRIADFSRIA
>Mature_686_residues
GVKELFLEIGTEEIPAGFIPRAMAEMEAMITRELTNARLSYSTVQTMATPRRLALVVKDIPAVQPDAEITATGPSKKAAF
DADGKPTKAAEGFARGQGVTVDQLQIVTTDKGEYLAVTKTETGRPTPELLSEILPAMIASIPFKKSMRWGDLDVRFARPI
HWIVALFDGIVVPFVFGNIESGSVSRGHRFMANTSFPVRNFSHYLDECERHFVIVDPERRKELIRRESHRVAAAAGGHLL
PDEELLEQITYLVEYPSAVHGTFSREFLKVPKEVLITSMRSHQRYFSIVDGQGNLMPGFITINNTLTDDPGVVVKGNERV
LRARLSDARFFFEEDQKVKLDERVEELKKVVYQQKLGTSFEKMERFRALAEELADRLNPAVKSETSRAAWLCKADLVSGM
VGEFPEVQGIMGREYALLEGESGDVACAIAEHYLPIQAGGELPASDIGAFVSIADKLDTICGCFSVGLIPTGAADPYALR
RATIGIIAIILDRGYRLGLASLIDRSLDLLEAKLLRPKEQVATEVLEFFRGRFVNLLGGEFDADAVEAAVSAEFDDLVDV
KSRVAALAQFKSHPDFEPLAVAFKRIGNIIKGGTDALVDPSLFQDAAEGALYQAFHEVKVHVSALVEQGAWQDALTRIAG
LRAPVDGFFDKVMVMAEDQRVRSNRLALLTAIDRMFGRIADFSRIA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=690, Percent_Identity=40, Blast_Score=483, Evalue=1e-137,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75752; Mature: 75621

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVKELFLEIGTEEIPAGFIPRAMAEMEAMITRELTNARLSYSTVQTMATPRRLALVVKD
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IPAVQPDAEITATGPSKKAAFDADGKPTKAAEGFARGQGVTVDQLQIVTTDKGEYLAVTK
CCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEEEEECCCCEEEEEE
TETGRPTPELLSEILPAMIASIPFKKSMRWGDLDVRFARPIHWIVALFDGIVVPFVFGNI
CCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCC
ESGSVSRGHRFMANTSFPVRNFSHYLDECERHFVIVDPERRKELIRRESHRVAAAAGGHL
CCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCEEHHCCCCC
LPDEELLEQITYLVEYPSAVHGTFSREFLKVPKEVLITSMRSHQRYFSIVDGQGNLMPGF
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCEE
ITINNTLTDDPGVVVKGNERVLRARLSDARFFFEEDQKVKLDERVEELKKVVYQQKLGTS
EEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
FEKMERFRALAEELADRLNPAVKSETSRAAWLCKADLVSGMVGEFPEVQGIMGREYALLE
HHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEE
GESGDVACAIAEHYLPIQAGGELPASDIGAFVSIADKLDTICGCFSVGLIPTGAADPYAL
CCCCCEEEEEHHHCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RRATIGIIAIILDRGYRLGLASLIDRSLDLLEAKLLRPKEQVATEVLEFFRGRFVNLLGG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
EFDADAVEAAVSAEFDDLVDVKSRVAALAQFKSHPDFEPLAVAFKRIGNIIKGGTDALVD
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PSLFQDAAEGALYQAFHEVKVHVSALVEQGAWQDALTRIAGLRAPVDGFFDKVMVMAEDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCH
RVRSNRLALLTAIDRMFGRIADFSRIA
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GVKELFLEIGTEEIPAGFIPRAMAEMEAMITRELTNARLSYSTVQTMATPRRLALVVKD
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IPAVQPDAEITATGPSKKAAFDADGKPTKAAEGFARGQGVTVDQLQIVTTDKGEYLAVTK
CCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEEEEECCCCEEEEEE
TETGRPTPELLSEILPAMIASIPFKKSMRWGDLDVRFARPIHWIVALFDGIVVPFVFGNI
CCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCC
ESGSVSRGHRFMANTSFPVRNFSHYLDECERHFVIVDPERRKELIRRESHRVAAAAGGHL
CCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCEEHHCCCCC
LPDEELLEQITYLVEYPSAVHGTFSREFLKVPKEVLITSMRSHQRYFSIVDGQGNLMPGF
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCEE
ITINNTLTDDPGVVVKGNERVLRARLSDARFFFEEDQKVKLDERVEELKKVVYQQKLGTS
EEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
FEKMERFRALAEELADRLNPAVKSETSRAAWLCKADLVSGMVGEFPEVQGIMGREYALLE
HHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEE
GESGDVACAIAEHYLPIQAGGELPASDIGAFVSIADKLDTICGCFSVGLIPTGAADPYAL
CCCCCEEEEEHHHCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RRATIGIIAIILDRGYRLGLASLIDRSLDLLEAKLLRPKEQVATEVLEFFRGRFVNLLGG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
EFDADAVEAAVSAEFDDLVDVKSRVAALAQFKSHPDFEPLAVAFKRIGNIIKGGTDALVD
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PSLFQDAAEGALYQAFHEVKVHVSALVEQGAWQDALTRIAGLRAPVDGFFDKVMVMAEDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCH
RVRSNRLALLTAIDRMFGRIADFSRIA
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA