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The map label for this gene is rpfG [H]

Identifier: 118581451

GI number: 118581451

Start: 3329836

End: 3330933

Strand: Direct

Name: rpfG [H]

Synonym: Ppro_3046

Alternate gene names: 118581451

Gene position: 3329836-3330933 (Clockwise)

Preceding gene: 118581450

Following gene: 118581468

Centisome position: 83.08

GC content: 52.91

Gene sequence:

>1098_bases
ATGCACTCTGAATCACCAGGAAACATCTTTATAGTAGATGACGACCGATATGTTCTCGAGAGTGTCAGCATACTGCTGCG
TGAATCTGGTTTCTCCGTAACCTCATTCAGCAGCGGGCACGACGCCATCAGGCAATTTGTAACTGAACCGGTGGATCTGA
TTCTGACCGATATCAACATGCCCGGCATGACAGGAATCGAGTTACTGGAAAAGATCCGCTATCTGGACCGAGAAACACCG
GTCCTGCTGATGACTGCCTATGCCGACCTGGATACCGCAGTGCGGGCTATTCAAAAAGGGGCCTTTGATTTTATCATCAA
ACCGTATCGCCCCCCCTACTTGATCCATGCGGTTGAAAAGGGGCTCACCTACAAGCGCCTGACTAACATTGAAAAGAACT
ACAAGCGCGAACTGGAGAAGACGGTTGAGCTGAGAACGTCAGAGTTGAACGATGCGCTCAACAAGCTGACTAACATGAGT
GGAGAAATCATCGAGCGGCTCACCGCCGCCGCTGAACTGCGCGACGAGGACACCGGCGCCCACATTAACCGTATCGGAAA
GTACGCCCAATGCCTCTCCTCTCAGCTCGGCCTTTCCGCGTCTTTCACGAAAAACATAACCGTTGCCAGCGCGATGCACG
ATGTCGGCAAGATCGGCATTCCTGACTCAATTCTATTGAAGCCTGCGGCACTCACCGCTGAAGAGTTCAACTGCATCAAA
CAGCATACCGTCATCGGCGAGAAGATTCTGCAGGGTTCTTCCCATGCCATGCTGCAGATGGGTGCGGTCATTGCTGCTAC
TCATCATGAACGCTGGGATGGGACGGGATATCCTTATGGGTTGAAGGCAGAGGCAATACCCATGGAAGGACGTCTCGTCA
TGCTGGCTGACCAATACGACGCCCTACGCAACAGCCGTGTCTATAAGCCAGCCTTCAGTCACGCCAAGACGTGCGACATA
CTGTTGAACGGTGACGGCCGGACGTTGCCGGAGCATTTTGAACCGCGTGTTCTGGATGCTTTCCGGGAGAGCGAGAAGGT
GTTTGAGGAGATTTTTGAAACCAGCCTGGCCCCGTCGATGGAAGACGATAGGGCTTAA

Upstream 100 bases:

>100_bases
ATACTCAAACCGCTCCTGTCCGTTGACAGAGACCTGCATGTCCTTACGAAACCGTTTCCTCCAAAGGAATTGCTTATGAA
AATCAGTGAGGTAGTCAAAG

Downstream 100 bases:

>100_bases
TCAAAGCAACTATTTAGATGTATTTACCGAGGTTTTTTAAACGCAAAGCAGCCCTATCACTTTGTAGTGATAGGGCTGTT
ATTTATTATTCCCGGCGGCG

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MHSESPGNIFIVDDDRYVLESVSILLRESGFSVTSFSSGHDAIRQFVTEPVDLILTDINMPGMTGIELLEKIRYLDRETP
VLLMTAYADLDTAVRAIQKGAFDFIIKPYRPPYLIHAVEKGLTYKRLTNIEKNYKRELEKTVELRTSELNDALNKLTNMS
GEIIERLTAAAELRDEDTGAHINRIGKYAQCLSSQLGLSASFTKNITVASAMHDVGKIGIPDSILLKPAALTAEEFNCIK
QHTVIGEKILQGSSHAMLQMGAVIAATHHERWDGTGYPYGLKAEAIPMEGRLVMLADQYDALRNSRVYKPAFSHAKTCDI
LLNGDGRTLPEHFEPRVLDAFRESEKVFEEIFETSLAPSMEDDRA

Sequences:

>Translated_365_residues
MHSESPGNIFIVDDDRYVLESVSILLRESGFSVTSFSSGHDAIRQFVTEPVDLILTDINMPGMTGIELLEKIRYLDRETP
VLLMTAYADLDTAVRAIQKGAFDFIIKPYRPPYLIHAVEKGLTYKRLTNIEKNYKRELEKTVELRTSELNDALNKLTNMS
GEIIERLTAAAELRDEDTGAHINRIGKYAQCLSSQLGLSASFTKNITVASAMHDVGKIGIPDSILLKPAALTAEEFNCIK
QHTVIGEKILQGSSHAMLQMGAVIAATHHERWDGTGYPYGLKAEAIPMEGRLVMLADQYDALRNSRVYKPAFSHAKTCDI
LLNGDGRTLPEHFEPRVLDAFRESEKVFEEIFETSLAPSMEDDRA
>Mature_365_residues
MHSESPGNIFIVDDDRYVLESVSILLRESGFSVTSFSSGHDAIRQFVTEPVDLILTDINMPGMTGIELLEKIRYLDRETP
VLLMTAYADLDTAVRAIQKGAFDFIIKPYRPPYLIHAVEKGLTYKRLTNIEKNYKRELEKTVELRTSELNDALNKLTNMS
GEIIERLTAAAELRDEDTGAHINRIGKYAQCLSSQLGLSASFTKNITVASAMHDVGKIGIPDSILLKPAALTAEEFNCIK
QHTVIGEKILQGSSHAMLQMGAVIAATHHERWDGTGYPYGLKAEAIPMEGRLVMLADQYDALRNSRVYKPAFSHAKTCDI
LLNGDGRTLPEHFEPRVLDAFRESEKVFEEIFETSLAPSMEDDRA

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=132, Percent_Identity=35.6060606060606, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790437, Length=117, Percent_Identity=39.3162393162393, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788550, Length=119, Percent_Identity=38.655462184874, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788905, Length=120, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082012, Length=178, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789402, Length=102, Percent_Identity=30.3921568627451, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786599, Length=110, Percent_Identity=30.9090909090909, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 40751; Mature: 40751

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSESPGNIFIVDDDRYVLESVSILLRESGFSVTSFSSGHDAIRQFVTEPVDLILTDINM
CCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHCCCHHEEEECCCC
PGMTGIELLEKIRYLDRETPVLLMTAYADLDTAVRAIQKGAFDFIIKPYRPPYLIHAVEK
CCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHHHH
GLTYKRLTNIEKNYKRELEKTVELRTSELNDALNKLTNMSGEIIERLTAAAELRDEDTGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCH
HINRIGKYAQCLSSQLGLSASFTKNITVASAMHDVGKIGIPDSILLKPAALTAEEFNCIK
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCHHHCHHHHHHHH
QHTVIGEKILQGSSHAMLQMGAVIAATHHERWDGTGYPYGLKAEAIPMEGRLVMLADQYD
HHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHH
ALRNSRVYKPAFSHAKTCDILLNGDGRTLPEHFEPRVLDAFRESEKVFEEIFETSLAPSM
HHHCCCCCCHHHCCCCEEEEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EDDRA
CCCCC
>Mature Secondary Structure
MHSESPGNIFIVDDDRYVLESVSILLRESGFSVTSFSSGHDAIRQFVTEPVDLILTDINM
CCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHCCCHHEEEECCCC
PGMTGIELLEKIRYLDRETPVLLMTAYADLDTAVRAIQKGAFDFIIKPYRPPYLIHAVEK
CCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHHHH
GLTYKRLTNIEKNYKRELEKTVELRTSELNDALNKLTNMSGEIIERLTAAAELRDEDTGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCH
HINRIGKYAQCLSSQLGLSASFTKNITVASAMHDVGKIGIPDSILLKPAALTAEEFNCIK
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCHHHCHHHHHHHH
QHTVIGEKILQGSSHAMLQMGAVIAATHHERWDGTGYPYGLKAEAIPMEGRLVMLADQYD
HHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHH
ALRNSRVYKPAFSHAKTCDILLNGDGRTLPEHFEPRVLDAFRESEKVFEEIFETSLAPSM
HHHCCCCCCHHHCCCCEEEEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EDDRA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]