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The map label for this gene is dusB [H]

Identifier: 118581317

GI number: 118581317

Start: 3186569

End: 3187597

Strand: Direct

Name: dusB [H]

Synonym: Ppro_2912

Alternate gene names: 118581317

Gene position: 3186569-3187597 (Clockwise)

Preceding gene: 118581316

Following gene: 118581318

Centisome position: 79.51

GC content: 63.27

Gene sequence:

>1029_bases
ATGACCCCATCCCCAACAACAGACCGCGACAACCCGACGCCACCACGTCTCCTGCCCGGGCCAGCGCACCCCCCGACGCT
CCCCTGGCGCCAGGGGCAGACTCCGCTCATGCTCGCTCCCATGCAGGGGGTGACCAACAGCGCCCTGCGCTCCCTGTTCA
TCGACTGGGTCCGACCGGATGTGGTCTTCACCGAATTCATGCGCGTCAACCCCGCTGCCGCACAGAGACGCCTCTCGGCG
GCCGACCTGGCCGAGGCGGGTTCAGCCGGAGATGATGTCCCCCTTGTGGTGCAACTGATCGGCCATGGCAGGGAAGCGCT
CGTTTCGGGCAGTCAGGCGGCCCAGGATGCCGGAGCAGCGCACATCAACCTGAACATGGGCTGTCCCTACGGCCGCATGA
CCAGCGGCCAGACCGGCGGCGGTATGCTGCGCTCCCCCCAGGAACTTTCAAGCATCATACCTGCACTGCGCAAGACCATC
AGGGGAACTTTCTCCATCAAACTGCGCTCCGGTTACGACAACCCCCGTCAGATATTCGACCTGCTGCCGCTCTTCGAAGC
ATCGGGAGTGGATTTCCTAGTTCTGCATCCCCGCACAGTACTGCAGAAGTACGCTGGTTTCGCCGACCACGGCATCACGG
CCGAAGCGGTCCAAAGGAGCACCCTGCCGATCATCGCCAACGGGGATATCCGCAGCGTAGCAACGGGGCGGCATGTGCTG
CGGGAAACCGGCGCAGCCGGACTGATGCTGGGGCGGGGCGCCATATCCGACCCCCTGCTCTTCCAGCGCCTGCGCAACTG
CGCCCCTCCCGACCCGCAACCACAGGAACGGGCGGCGATGCTCTCCCGCTATCTGGATGAGTTGCTGACCCGCTACCAGA
CCCTGTTCTGCGGTGAAGCCCAGATACTGGGTAAAATCAAGGAGATCGTATCCACCATCCATGAACCTGCCTTTGAAAAA
GGCTTCCATCGGCTAAAAAAGGCCCGCAGAATCGAGGCCTTCAGGGAGCTTTTGGCGGAACTGGCCTGA

Upstream 100 bases:

>100_bases
AGAGATCTTCTAGAGAGTTCCCCCCACGGATTCACTGCACAACGACTTCCGGGGCCGTGCCCGGGAGTCGTTGTTTTTTT
GCGTCCGCACCATCGCCCCC

Downstream 100 bases:

>100_bases
CCCTTGGAAACGCAACCCACCTTCGACCACCAGAAACTGCTGGATCTTGCCGCAATGCGCATGCCCTTTGGAAAGTATGC
CGGGCGCTTGCTGATCGACC

Product: dihydrouridine synthase, DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTPSPTTDRDNPTPPRLLPGPAHPPTLPWRQGQTPLMLAPMQGVTNSALRSLFIDWVRPDVVFTEFMRVNPAAAQRRLSA
ADLAEAGSAGDDVPLVVQLIGHGREALVSGSQAAQDAGAAHINLNMGCPYGRMTSGQTGGGMLRSPQELSSIIPALRKTI
RGTFSIKLRSGYDNPRQIFDLLPLFEASGVDFLVLHPRTVLQKYAGFADHGITAEAVQRSTLPIIANGDIRSVATGRHVL
RETGAAGLMLGRGAISDPLLFQRLRNCAPPDPQPQERAAMLSRYLDELLTRYQTLFCGEAQILGKIKEIVSTIHEPAFEK
GFHRLKKARRIEAFRELLAELA

Sequences:

>Translated_342_residues
MTPSPTTDRDNPTPPRLLPGPAHPPTLPWRQGQTPLMLAPMQGVTNSALRSLFIDWVRPDVVFTEFMRVNPAAAQRRLSA
ADLAEAGSAGDDVPLVVQLIGHGREALVSGSQAAQDAGAAHINLNMGCPYGRMTSGQTGGGMLRSPQELSSIIPALRKTI
RGTFSIKLRSGYDNPRQIFDLLPLFEASGVDFLVLHPRTVLQKYAGFADHGITAEAVQRSTLPIIANGDIRSVATGRHVL
RETGAAGLMLGRGAISDPLLFQRLRNCAPPDPQPQERAAMLSRYLDELLTRYQTLFCGEAQILGKIKEIVSTIHEPAFEK
GFHRLKKARRIEAFRELLAELA
>Mature_341_residues
TPSPTTDRDNPTPPRLLPGPAHPPTLPWRQGQTPLMLAPMQGVTNSALRSLFIDWVRPDVVFTEFMRVNPAAAQRRLSAA
DLAEAGSAGDDVPLVVQLIGHGREALVSGSQAAQDAGAAHINLNMGCPYGRMTSGQTGGGMLRSPQELSSIIPALRKTIR
GTFSIKLRSGYDNPRQIFDLLPLFEASGVDFLVLHPRTVLQKYAGFADHGITAEAVQRSTLPIIANGDIRSVATGRHVLR
ETGAAGLMLGRGAISDPLLFQRLRNCAPPDPQPQERAAMLSRYLDELLTRYQTLFCGEAQILGKIKEIVSTIHEPAFEKG
FHRLKKARRIEAFRELLAELA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=243, Percent_Identity=27.5720164609054, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI239788483, Length=173, Percent_Identity=26.0115606936416, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI239788462, Length=173, Percent_Identity=26.0115606936416, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1788462, Length=256, Percent_Identity=29.296875, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789660, Length=240, Percent_Identity=27.9166666666667, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25144369, Length=207, Percent_Identity=26.5700483091787, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19921524, Length=192, Percent_Identity=31.7708333333333, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585320, Length=203, Percent_Identity=29.064039408867, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24580595, Length=174, Percent_Identity=26.4367816091954, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920448, Length=174, Percent_Identity=26.4367816091954, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37246; Mature: 37115

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSPTTDRDNPTPPRLLPGPAHPPTLPWRQGQTPLMLAPMQGVTNSALRSLFIDWVRPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCHH
VVFTEFMRVNPAAAQRRLSAADLAEAGSAGDDVPLVVQLIGHGREALVSGSQAAQDAGAA
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHCCCHHHHHCCCE
HINLNMGCPYGRMTSGQTGGGMLRSPQELSSIIPALRKTIRGTFSIKLRSGYDNPRQIFD
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHH
LLPLFEASGVDFLVLHPRTVLQKYAGFADHGITAEAVQRSTLPIIANGDIRSVATGRHVL
HHHHHHCCCCCEEEECCHHHHHHHCCHHCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHH
RETGAAGLMLGRGAISDPLLFQRLRNCAPPDPQPQERAAMLSRYLDELLTRYQTLFCGEA
HHCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
QILGKIKEIVSTIHEPAFEKGFHRLKKARRIEAFRELLAELA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TPSPTTDRDNPTPPRLLPGPAHPPTLPWRQGQTPLMLAPMQGVTNSALRSLFIDWVRPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCHH
VVFTEFMRVNPAAAQRRLSAADLAEAGSAGDDVPLVVQLIGHGREALVSGSQAAQDAGAA
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHCCCHHHHHCCCE
HINLNMGCPYGRMTSGQTGGGMLRSPQELSSIIPALRKTIRGTFSIKLRSGYDNPRQIFD
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHH
LLPLFEASGVDFLVLHPRTVLQKYAGFADHGITAEAVQRSTLPIIANGDIRSVATGRHVL
HHHHHHCCCCCEEEECCHHHHHHHCCHHCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHH
RETGAAGLMLGRGAISDPLLFQRLRNCAPPDPQPQERAAMLSRYLDELLTRYQTLFCGEA
HHCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
QILGKIKEIVSTIHEPAFEKGFHRLKKARRIEAFRELLAELA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]