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The map label for this gene is pcm2

Identifier: 118581244

GI number: 118581244

Start: 3104537

End: 3105214

Strand: Reverse

Name: pcm2

Synonym: Ppro_2838

Alternate gene names: 118581244

Gene position: 3105214-3104537 (Counterclockwise)

Preceding gene: 118581246

Following gene: 118581243

Centisome position: 77.48

GC content: 62.98

Gene sequence:

>678_bases
ATGCGCTACCGTTGTCCTATGAATTGTCCTTCTCTCAGAAATCAGGAGTACCGGCGCGAGATCATGATCAGCGAACAGCT
GATGGGGCGCGGAATCCGTGACCCCGCCGTGCTGGCGGCCATGCGTCAGGTGCCCCGCGAGGCGTTCGTCGATCAGGGGA
TGCGGGAACTGGCCTACGAGGACCATCCGCTGCCCATCGACGGAGGGCAGACCATCTCGCAGCCCTACATCGTGGCTTAC
ATGACCGAAGCGCTTGAATTGTCGAGTTCGGACCGGGTCCTGGAGATCGGCACCGGTTCGGGCTATGCCGCTGCTGTGCT
CAGCCGCATCGTCCATACCGTCTACAGCGTGGAGCGTCTGGAGGAGTTGGCCCGCGGCGCACGCCAGCGCCTGGAGAGCC
TGGGCTACAACAATGTTGAAGTCTTTGAAGGGGACGGTACCCTGGGGTGGCCGGAGCATGCCCCCTACGATGCCATCGTT
GTCACGGCCGGGGCGCCCGCTGTACCGAAGCCGCTCCTCCGGCAGCTTGTCGTGGGGGGGCGGCTGGTCATTCCGGTGGG
TGCATCGTCATTTCTGCAGAATCTCATCCGTGTTCGCCGGCAGGGGGAAGATGACTATCGCAGCGAAGAACTCTGTGGAG
TTCGTTTCGTTCCCCTGGTGGGGGCAGAGGGGTGGTGA

Upstream 100 bases:

>100_bases
CATGGCGGGGATATGTGCCAGAGTATATCAGACGGCGCCGCGGTCGCCATCACTGCCCCGTTGTGGCGGACCGGCCGCCC
CCGATTGCTAATTGCCGGTA

Downstream 100 bases:

>100_bases
GCGGACAGCCCCTCAAGCCATAAGCTGATTGTCAGGTCGTAAACCGCCAACCCAGAAGCACATCCATAGAAGGGGGTATC
CCATGAACCTTGAAATGCAT

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 2; Protein L-isoaspartyl methyltransferase 2; Protein-beta-aspartate methyltransferase 2; PIMT 2

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MRYRCPMNCPSLRNQEYRREIMISEQLMGRGIRDPAVLAAMRQVPREAFVDQGMRELAYEDHPLPIDGGQTISQPYIVAY
MTEALELSSSDRVLEIGTGSGYAAAVLSRIVHTVYSVERLEELARGARQRLESLGYNNVEVFEGDGTLGWPEHAPYDAIV
VTAGAPAVPKPLLRQLVVGGRLVIPVGASSFLQNLIRVRRQGEDDYRSEELCGVRFVPLVGAEGW

Sequences:

>Translated_225_residues
MRYRCPMNCPSLRNQEYRREIMISEQLMGRGIRDPAVLAAMRQVPREAFVDQGMRELAYEDHPLPIDGGQTISQPYIVAY
MTEALELSSSDRVLEIGTGSGYAAAVLSRIVHTVYSVERLEELARGARQRLESLGYNNVEVFEGDGTLGWPEHAPYDAIV
VTAGAPAVPKPLLRQLVVGGRLVIPVGASSFLQNLIRVRRQGEDDYRSEELCGVRFVPLVGAEGW
>Mature_225_residues
MRYRCPMNCPSLRNQEYRREIMISEQLMGRGIRDPAVLAAMRQVPREAFVDQGMRELAYEDHPLPIDGGQTISQPYIVAY
MTEALELSSSDRVLEIGTGSGYAAAVLSRIVHTVYSVERLEELARGARQRLESLGYNNVEVFEGDGTLGWPEHAPYDAIV
VTAGAPAVPKPLLRQLVVGGRLVIPVGASSFLQNLIRVRRQGEDDYRSEELCGVRFVPLVGAEGW

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=175, Percent_Identity=35.4285714285714, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1789100, Length=206, Percent_Identity=50.4854368932039, Blast_Score=184, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI71983477, Length=180, Percent_Identity=34.4444444444444, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI193207222, Length=178, Percent_Identity=33.1460674157303, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17981723, Length=211, Percent_Identity=34.1232227488152, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT2_PELPD (A1ASW6)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902494.1
- ProteinModelPortal:   A1ASW6
- SMR:   A1ASW6
- STRING:   A1ASW6
- GeneID:   4574858
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_2838
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   LCPVRFV
- PhylomeDB:   A1ASW6
- ProtClustDB:   CLSK850223
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 25003; Mature: 25003

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 73-73

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYRCPMNCPSLRNQEYRREIMISEQLMGRGIRDPAVLAAMRQVPREAFVDQGMRELAYE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DHPLPIDGGQTISQPYIVAYMTEALELSSSDRVLEIGTGSGYAAAVLSRIVHTVYSVERL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
EELARGARQRLESLGYNNVEVFEGDGTLGWPEHAPYDAIVVTAGAPAVPKPLLRQLVVGG
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCC
RLVIPVGASSFLQNLIRVRRQGEDDYRSEELCGVRFVPLVGAEGW
EEEEECCHHHHHHHHHHHHHCCCCHHCHHHHCCEEEEECCCCCCC
>Mature Secondary Structure
MRYRCPMNCPSLRNQEYRREIMISEQLMGRGIRDPAVLAAMRQVPREAFVDQGMRELAYE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DHPLPIDGGQTISQPYIVAYMTEALELSSSDRVLEIGTGSGYAAAVLSRIVHTVYSVERL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
EELARGARQRLESLGYNNVEVFEGDGTLGWPEHAPYDAIVVTAGAPAVPKPLLRQLVVGG
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCC
RLVIPVGASSFLQNLIRVRRQGEDDYRSEELCGVRFVPLVGAEGW
EEEEECCHHHHHHHHHHHHHCCCCHHCHHHHCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA