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The map label for this gene is ygfT [H]

Identifier: 118581236

GI number: 118581236

Start: 3096556

End: 3097965

Strand: Direct

Name: ygfT [H]

Synonym: Ppro_2830

Alternate gene names: 118581236

Gene position: 3096556-3097965 (Clockwise)

Preceding gene: 118581235

Following gene: 118581239

Centisome position: 77.26

GC content: 63.97

Gene sequence:

>1410_bases
ATGACAGCCGATATGACCATCAAGGAGCGACTGGCCATCAAGCGGGTCCATATGTCCGAGCGGGATGCCGGGGAGCGGAG
CAGGGCCTTTGTCGAGGTAAACCAGGGGCTCTCCCTGGAGGACGCTATCCGCGAAGCCCAGCGCTGCCTGCAGTGCAAGA
CGCGTCCCTGCGTGGCGGGATGCCCGGTCAGCGTTTCCATCCCTGAATTCATCGCCAAACTGGCCGACAACGACCTGGTG
GCAGCTGCCCGCATCCTGCGCGGCGACAATGCGCTTCCCGCAGTCTGTGGCCGGGTCTGCCCCCAGGAAACCCAGTGCGA
GGAGAAGTGCGTCTGCGGGATCAAGGGAGATCCGGTCGCCATCGGCTATCTGGAGAGGTTCGTCGCCGACTGGGCCATGG
AACACTCCGACCAGCTGGAACAAGAGATACTGCCCCCGCCCACCGGCAAGCGGGTCGCCGTTGTCGGCAGCGGCCCCGCC
GGCTTGACCGCGGCCGGAGAACTGGCCCGTGCCGGACATGACGTGACCATCTTCGAGGCACTGCACGACACCGGCGGCGT
CCTGCGCTACGGCATCCCCGAGTTCCGTCTGCCCAACGACATCATCGACGCGGAAGTCAATTGCCTGCGTACCCTGGGCG
TCAAGATCGAGCTCAACGTCATCATCGGCAAGACCCTGACCCTGCCCCAGTTGCAGGCCGAATTCGACGCCCTCTTCATC
GCCAATGGCGCCGGCCTGCCGGTGATGCTCAACATTCCCGGCGAAAACCTGAAGGGGGTCTATGCGGCCAACGAATACCT
GACCAGGGTCAACCTGATGGGAGCGGGGCGTTCCCAAGATGCCGCCACGCCGATCATTAAGGGAAAGAACGTTACGGTCA
TCGGCGGCGGCAACACGGCCATGGATTGCGTACGCACCGCCAAGCGCCTGGGCGCCGAGCGGGCCATGATCGTCTACCGT
CGCACCGAAGCGGAAATGCCGGCCCGTGCCGAGGAGATCAAGCACGCCAAGGAGGAGGGGGTGGAGTTCCTGCTACTCAC
CGCTCCAGTGGCCATCCTCGATGAAAACGGCTGGGTCGCGAAACTTTCCTGTCAGCATATGGAGTTGGGGGAACCGGATG
ATTCGGGTCGGCGCAGACCGGTTCCGGTGGACGGCGCCTATTTCGACCTGCCGGCCGACGTGGTGGTCAACGCCATCGGC
ACCCGGGCCAACCCGCTGCTGACCGCCACCGCCCCTGATTTGAAGCTGAACCGCTGGGGCAACATCCAGACCGATGAAAA
CGGCTTGACCAGTCTTGAAGGTGTCTTCGCGGGCGGAGATATCGTCAGGGGGGGGGCCACGGTGATCCTCGCCATGGGTG
ACGGGAAGCAGGCCGCGGCGGCGATAAACCGCTATCTGGCATCAAAATAG

Upstream 100 bases:

>100_bases
CCCACAAGGTAGATTTCGCCAACCTGATCGATCGTCTCGGCACCTACCGTGAACATGAGGCCGCATCCGCAGAGTGCCGG
CTGAAACAGGAGGTGAAGCC

Downstream 100 bases:

>100_bases
CGCCACAAAAACAAAAACAGCATTTTATCCGCTTGATCTACACGGGACTGCCGCAGGGCATGTCCCGTTTTTTATGCGCG
GGCATCTATGGGAGGAGGCG

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTADMTIKERLAIKRVHMSERDAGERSRAFVEVNQGLSLEDAIREAQRCLQCKTRPCVAGCPVSVSIPEFIAKLADNDLV
AAARILRGDNALPAVCGRVCPQETQCEEKCVCGIKGDPVAIGYLERFVADWAMEHSDQLEQEILPPPTGKRVAVVGSGPA
GLTAAGELARAGHDVTIFEALHDTGGVLRYGIPEFRLPNDIIDAEVNCLRTLGVKIELNVIIGKTLTLPQLQAEFDALFI
ANGAGLPVMLNIPGENLKGVYAANEYLTRVNLMGAGRSQDAATPIIKGKNVTVIGGGNTAMDCVRTAKRLGAERAMIVYR
RTEAEMPARAEEIKHAKEEGVEFLLLTAPVAILDENGWVAKLSCQHMELGEPDDSGRRRPVPVDGAYFDLPADVVVNAIG
TRANPLLTATAPDLKLNRWGNIQTDENGLTSLEGVFAGGDIVRGGATVILAMGDGKQAAAAINRYLASK

Sequences:

>Translated_469_residues
MTADMTIKERLAIKRVHMSERDAGERSRAFVEVNQGLSLEDAIREAQRCLQCKTRPCVAGCPVSVSIPEFIAKLADNDLV
AAARILRGDNALPAVCGRVCPQETQCEEKCVCGIKGDPVAIGYLERFVADWAMEHSDQLEQEILPPPTGKRVAVVGSGPA
GLTAAGELARAGHDVTIFEALHDTGGVLRYGIPEFRLPNDIIDAEVNCLRTLGVKIELNVIIGKTLTLPQLQAEFDALFI
ANGAGLPVMLNIPGENLKGVYAANEYLTRVNLMGAGRSQDAATPIIKGKNVTVIGGGNTAMDCVRTAKRLGAERAMIVYR
RTEAEMPARAEEIKHAKEEGVEFLLLTAPVAILDENGWVAKLSCQHMELGEPDDSGRRRPVPVDGAYFDLPADVVVNAIG
TRANPLLTATAPDLKLNRWGNIQTDENGLTSLEGVFAGGDIVRGGATVILAMGDGKQAAAAINRYLASK
>Mature_468_residues
TADMTIKERLAIKRVHMSERDAGERSRAFVEVNQGLSLEDAIREAQRCLQCKTRPCVAGCPVSVSIPEFIAKLADNDLVA
AARILRGDNALPAVCGRVCPQETQCEEKCVCGIKGDPVAIGYLERFVADWAMEHSDQLEQEILPPPTGKRVAVVGSGPAG
LTAAGELARAGHDVTIFEALHDTGGVLRYGIPEFRLPNDIIDAEVNCLRTLGVKIELNVIIGKTLTLPQLQAEFDALFIA
NGAGLPVMLNIPGENLKGVYAANEYLTRVNLMGAGRSQDAATPIIKGKNVTVIGGGNTAMDCVRTAKRLGAERAMIVYRR
TEAEMPARAEEIKHAKEEGVEFLLLTAPVAILDENGWVAKLSCQHMELGEPDDSGRRRPVPVDGAYFDLPADVVVNAIGT
RANPLLTATAPDLKLNRWGNIQTDENGLTSLEGVFAGGDIVRGGATVILAMGDGKQAAAAINRYLASK

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=456, Percent_Identity=33.3333333333333, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI237757300, Length=90, Percent_Identity=36.6666666666667, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI87082180, Length=445, Percent_Identity=38.2022471910112, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1788811, Length=452, Percent_Identity=36.9469026548673, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1789606, Length=468, Percent_Identity=35.6837606837607, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1788468, Length=432, Percent_Identity=36.8055555555556, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789243, Length=458, Percent_Identity=34.9344978165939, Blast_Score=207, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI71984108, Length=460, Percent_Identity=33.2608695652174, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17570289, Length=486, Percent_Identity=30.8641975308642, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17543860, Length=162, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=479, Percent_Identity=31.1064718162839, Blast_Score=199, Evalue=6e-52,
Organism=Drosophila melanogaster, GI28574881, Length=479, Percent_Identity=32.1503131524008, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24665539, Length=479, Percent_Identity=32.1503131524008, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24665547, Length=482, Percent_Identity=31.9502074688797, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24665543, Length=482, Percent_Identity=31.9502074688797, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24640763, Length=450, Percent_Identity=31.3333333333333, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI18858217, Length=450, Percent_Identity=31.3333333333333, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00037 Fer4; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 50178; Mature: 50047

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADMTIKERLAIKRVHMSERDAGERSRAFVEVNQGLSLEDAIREAQRCLQCKTRPCVAG
CCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCC
CPVSVSIPEFIAKLADNDLVAAARILRGDNALPAVCGRVCPQETQCEEKCVCGIKGDPVA
CCCEECHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHEEECCCCCCEE
IGYLERFVADWAMEHSDQLEQEILPPPTGKRVAVVGSGPAGLTAAGELARAGHDVTIFEA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEE
LHDTGGVLRYGIPEFRLPNDIIDAEVNCLRTLGVKIELNVIIGKTLTLPQLQAEFDALFI
HHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHCCCEEEE
ANGAGLPVMLNIPGENLKGVYAANEYLTRVNLMGAGRSQDAATPIIKGKNVTVIGGGNTA
ECCCCCEEEEECCCCCCCEEEEHHHHHHEEEEEECCCCCCCCCCEECCCEEEEEECCCHH
MDCVRTAKRLGAERAMIVYRRTEAEMPARAEEIKHAKEEGVEFLLLTAPVAILDENGWVA
HHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCEEEEECCCCEE
KLSCQHMELGEPDDSGRRRPVPVDGAYFDLPADVVVNAIGTRANPLLTATAPDLKLNRWG
EEEECCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHCCCCCCEEEECCCCCEECCCC
NIQTDENGLTSLEGVFAGGDIVRGGATVILAMGDGKQAAAAINRYLASK
CCCCCCCCCCHHHEEECCCCEEECCEEEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TADMTIKERLAIKRVHMSERDAGERSRAFVEVNQGLSLEDAIREAQRCLQCKTRPCVAG
CCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCC
CPVSVSIPEFIAKLADNDLVAAARILRGDNALPAVCGRVCPQETQCEEKCVCGIKGDPVA
CCCEECHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHEEECCCCCCEE
IGYLERFVADWAMEHSDQLEQEILPPPTGKRVAVVGSGPAGLTAAGELARAGHDVTIFEA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEE
LHDTGGVLRYGIPEFRLPNDIIDAEVNCLRTLGVKIELNVIIGKTLTLPQLQAEFDALFI
HHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHCCCEEEE
ANGAGLPVMLNIPGENLKGVYAANEYLTRVNLMGAGRSQDAATPIIKGKNVTVIGGGNTA
ECCCCCEEEEECCCCCCCEEEEHHHHHHEEEEEECCCCCCCCCCEECCCEEEEEECCCHH
MDCVRTAKRLGAERAMIVYRRTEAEMPARAEEIKHAKEEGVEFLLLTAPVAILDENGWVA
HHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCEEEEECCCCEE
KLSCQHMELGEPDDSGRRRPVPVDGAYFDLPADVVVNAIGTRANPLLTATAPDLKLNRWG
EEEECCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHCCCCCCEEEECCCCCEECCCC
NIQTDENGLTSLEGVFAGGDIVRGGATVILAMGDGKQAAAAINRYLASK
CCCCCCCCCCHHHEEECCCCEEECCEEEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]