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The map label for this gene is prfA

Identifier: 118581059

GI number: 118581059

Start: 2874660

End: 2875724

Strand: Reverse

Name: prfA

Synonym: Ppro_2647

Alternate gene names: 118581059

Gene position: 2875724-2874660 (Counterclockwise)

Preceding gene: 118581060

Following gene: 118581056

Centisome position: 71.75

GC content: 61.69

Gene sequence:

>1065_bases
ATGTTCGACAAGATAGAAGAACTTGAGCGGCGCTACCAGGAACTGGAAGCGCTGTTGTCCGACCCGGCGGTCATTTCCAA
CCAGCCGGAGTTTCGCAAGCTGTCCCGCGAACATGCCGACCTGACCGGCCTGGTGGCTGCCTACCGCCGCTACCGCAGGG
TGCTGGAGGAGATGGAGGGAAACCGCGAGCTGCTGGCTGATGTGGATATGAAGGAGATGGCGGAAGAGGAGCTGAAAGTT
CTGGAGGAGGAAAAGGAACGACTGGAGGGGGAAATCCAGATGCTGTTGCTTCCCAGGGATCCCAACGATGACAAGAGCGT
CATCCTGGAGATTCGCGCCGGCACCGGCGGCGACGAATCGGCTCTGTTCGCCGGCGACCTGTTTCGCATGTATTCCCGTT
TCGCGGACGTCAACCGCTGGAAGGTGGAGACCATATCCGCCTCGGAATCGGAGCGGGGCGGCTTCAAGGAGATCATCGCC
TCCGTGGAAGGGGAGGGGGTCTTTGCCAAGCTCAAGTACGAATCAGGTACCCACCGCGTGCAGCGCGTTCCGGAGACCGA
GGCCCAAGGGCGCATCCACACCAGCGCCTGCACCGTGGCGATCATGGCAGAGGCCGAGGACGTGGACATCGACATCAATC
CGACCGACCTGAAGATCGACGTGTACCGCTCCTCCGGCGCTGGTGGTCAGCATGTCAACACCACCGATTCAGCCGTGCGC
ATCACCCACCTTCCCACCGGCACGGTGGTGGCCTGCCAGGAGGAACGCAGCCAGATCAAGAACCGCGCCAAGGCCATGAA
GGTCCTCAAGACCCGCATCATGGACTCCATTCAGCAGGAACAGAACGCCAGGATGGCGGCGGACCGCAAGCAGCAGGTTG
GTTCCGGCGACCGCTCGGAGCGCATTCGCACCTATAACTTTCCCCAGGGACGCATGACCGATCATCGCATCGGCCTGACG
CTCTACCGCCTGGATGCCATCATGGCCGGGGATATCGCCGAGATCGTGGATGCCCTGCGCGCCCATTACCAGATGGAGGC
GCTCAAGGCGCAGAGTGAAGAGTGA

Upstream 100 bases:

>100_bases
ACGGTGCGCCTGGTCGCTTCAGTGCTCTGATCCGTTGCGTCCGTGAGGTGGCGTGAGTAACTGCCATTTTGAGATGATAT
TTTTGTGAGAGGTCTTTGTG

Downstream 100 bases:

>100_bases
AGAGTGAAGAGTGAAGAGTGAAGAGTGAAGAGTGAAGAGTGAAGAGTGAAGAGTGAAGAGTGAAGAGTGAAGAGTGAAGA
AAGCGCGGGGCGACCATCCG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MFDKIEELERRYQELEALLSDPAVISNQPEFRKLSREHADLTGLVAAYRRYRRVLEEMEGNRELLADVDMKEMAEEELKV
LEEEKERLEGEIQMLLLPRDPNDDKSVILEIRAGTGGDESALFAGDLFRMYSRFADVNRWKVETISASESERGGFKEIIA
SVEGEGVFAKLKYESGTHRVQRVPETEAQGRIHTSACTVAIMAEAEDVDIDINPTDLKIDVYRSSGAGGQHVNTTDSAVR
ITHLPTGTVVACQEERSQIKNRAKAMKVLKTRIMDSIQQEQNARMAADRKQQVGSGDRSERIRTYNFPQGRMTDHRIGLT
LYRLDAIMAGDIAEIVDALRAHYQMEALKAQSEE

Sequences:

>Translated_354_residues
MFDKIEELERRYQELEALLSDPAVISNQPEFRKLSREHADLTGLVAAYRRYRRVLEEMEGNRELLADVDMKEMAEEELKV
LEEEKERLEGEIQMLLLPRDPNDDKSVILEIRAGTGGDESALFAGDLFRMYSRFADVNRWKVETISASESERGGFKEIIA
SVEGEGVFAKLKYESGTHRVQRVPETEAQGRIHTSACTVAIMAEAEDVDIDINPTDLKIDVYRSSGAGGQHVNTTDSAVR
ITHLPTGTVVACQEERSQIKNRAKAMKVLKTRIMDSIQQEQNARMAADRKQQVGSGDRSERIRTYNFPQGRMTDHRIGLT
LYRLDAIMAGDIAEIVDALRAHYQMEALKAQSEE
>Mature_354_residues
MFDKIEELERRYQELEALLSDPAVISNQPEFRKLSREHADLTGLVAAYRRYRRVLEEMEGNRELLADVDMKEMAEEELKV
LEEEKERLEGEIQMLLLPRDPNDDKSVILEIRAGTGGDESALFAGDLFRMYSRFADVNRWKVETISASESERGGFKEIIA
SVEGEGVFAKLKYESGTHRVQRVPETEAQGRIHTSACTVAIMAEAEDVDIDINPTDLKIDVYRSSGAGGQHVNTTDSAVR
ITHLPTGTVVACQEERSQIKNRAKAMKVLKTRIMDSIQQEQNARMAADRKQQVGSGDRSERIRTYNFPQGRMTDHRIGLT
LYRLDAIMAGDIAEIVDALRAHYQMEALKAQSEE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=283, Percent_Identity=48.4098939929329, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI34577120, Length=337, Percent_Identity=39.7626112759644, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI166795305, Length=178, Percent_Identity=48.876404494382, Blast_Score=194, Evalue=2e-49,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=56.8181818181818, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI2367172, Length=332, Percent_Identity=37.0481927710843, Blast_Score=203, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=257, Percent_Identity=38.5214007782101, Blast_Score=180, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=318, Percent_Identity=39.622641509434, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI19921226, Length=325, Percent_Identity=41.8461538461538, Blast_Score=231, Evalue=8e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_PELPD (A1ASD1)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902309.1
- ProteinModelPortal:   A1ASD1
- SMR:   A1ASD1
- STRING:   A1ASD1
- GeneID:   4574319
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_2647
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   NTNEGEH
- PhylomeDB:   A1ASD1
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40066; Mature: 40066

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKIEELERRYQELEALLSDPAVISNQPEFRKLSREHADLTGLVAAYRRYRRVLEEMEG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NRELLADVDMKEMAEEELKVLEEEKERLEGEIQMLLLPRDPNDDKSVILEIRAGTGGDES
CHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCCCCC
ALFAGDLFRMYSRFADVNRWKVETISASESERGGFKEIIASVEGEGVFAKLKYESGTHRV
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCHHH
QRVPETEAQGRIHTSACTVAIMAEAEDVDIDINPTDLKIDVYRSSGAGGQHVNTTDSAVR
HCCCCCHHCCCEEECCEEEEEEECCCCCEEECCCCEEEEEEEECCCCCCCCCCCCCCEEE
ITHLPTGTVVACQEERSQIKNRAKAMKVLKTRIMDSIQQEQNARMAADRKQQVGSGDRSE
EEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RIRTYNFPQGRMTDHRIGLTLYRLDAIMAGDIAEIVDALRAHYQMEALKAQSEE
CCEEECCCCCCCCCHHHCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFDKIEELERRYQELEALLSDPAVISNQPEFRKLSREHADLTGLVAAYRRYRRVLEEMEG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NRELLADVDMKEMAEEELKVLEEEKERLEGEIQMLLLPRDPNDDKSVILEIRAGTGGDES
CHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECCCCCCCC
ALFAGDLFRMYSRFADVNRWKVETISASESERGGFKEIIASVEGEGVFAKLKYESGTHRV
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCHHH
QRVPETEAQGRIHTSACTVAIMAEAEDVDIDINPTDLKIDVYRSSGAGGQHVNTTDSAVR
HCCCCCHHCCCEEECCEEEEEEECCCCCEEECCCCEEEEEEEECCCCCCCCCCCCCCEEE
ITHLPTGTVVACQEERSQIKNRAKAMKVLKTRIMDSIQQEQNARMAADRKQQVGSGDRSE
EEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RIRTYNFPQGRMTDHRIGLTLYRLDAIMAGDIAEIVDALRAHYQMEALKAQSEE
CCEEECCCCCCCCCHHHCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA