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The map label for this gene is ilvB2 [H]

Identifier: 118580972

GI number: 118580972

Start: 2773871

End: 2775592

Strand: Direct

Name: ilvB2 [H]

Synonym: Ppro_2560

Alternate gene names: 118580972

Gene position: 2773871-2775592 (Clockwise)

Preceding gene: 118580964

Following gene: 118580973

Centisome position: 69.21

GC content: 65.1

Gene sequence:

>1722_bases
ATGGCACTGACGGTAAGCGATCTGATCGTGGAGTATCTGGAGCAGTTTGGCGTGGAGTACGTATTCGGCGTCCCGGGCAG
CCCGTTGGGGCCGCTGTTCGACGCCCTGGTGTGCAGCGAGAAACGGGGCGGCCCGCGGCTTGTGCTGGCCCGCCACGAGG
CGGGAGCGGCGTTCATGGCCGACGGCTATGCCCGGGAGAGCGGAAGGATCGGCGTCTGCTGCTCCACCACCGGGCCGGGC
GCCACCAACCTGATCACCGGCGTGGCCGGCGCTTATGCCGAGCAGGTGCCCATGCTGGTGCTCTCCTCCCAGACCAGGGT
GACCGATTTCAGCTTCGGCTGTTTCCAGGACTCTTCCCGGGACGGCGTGGACATCATGAGCATGTTCGCCTGCTGCACCC
GCTACAACTCCCTGGTGTCACACCCCAACCAGTTGGAGAAGAAGCTGGCCTCGGCCCTGACCACGGCGTTGGGCAGCCCC
CGGGGACCGGCCCACCTGAGCATCCCCATCGACATCTTCGGCGCCGATGCCAGTGGTCCAGCCTCTTATCCCGGACTGCA
CTCCCTGGTGAGCGCCGAGGAAAGCACCGTCGACGGCGCGGCTCTGGAGCGGTTGATGAATGAGATCACCACCGTACGGA
GCACTTCCGGCCGGATCGTCCTGCTGGCAGGGGCCAACGCCATCAGCGCAGGCGACCTGATCACACGCTTCGCCGAACTG
ACCGGGGCCAAGATCATCACCACCCCGCGGGGAAAACCGGCCATCAACCCCTATCATCCCTTGGCGCGCGGCGTGTTCGG
TGTTTCCGGGCACAGTTCGGCCCGCCAGACACTGGCGGAGGATAACGTCCAGCTGATCTTGGCCGTGGGGAGCAACCTGG
GGGAATGGGAGACCAGCAAATGGGACCCAATCCTGATGAACGATTGCATGGTGCACATCGACAGCGACCGCCAGAACTTC
ACCCGCTCCCCCATGGCCAGACTGCACCTCTACGGGGGGATTCATGCCATCTTCGAACAGTTGAACCGCCGACTGGAGCA
ACATGACGGCTCGGCCGTCTCCCGTTCCAAAGGGTCCGCTGAAACCGCTCCCGCGACGAGCGCCTATGTGCCGGCGGGAA
TCAGCGTGCGCAACGCCGACTGCTGCGCTCCTCCTGCCAGCGGCCAGCTGCTCAAGGCGCCCCAGGTGTACGTGGAGCTA
ATCCAGCGCCTGCCGCGGGAGAGCCGTTTCTTCATCGACAACAGCAACAGCGTGCCCTGGTCCATCCACTACTTCTTCCA
CCAGCGACCGGAGGCGCTGCACTACTCCATCGAATTCGCCACCATGGCCTGGGCCGTGGGCGCCGCGGTGGGAGGAGCAT
TCGCCAATCAAAAGGCGCCGTCAGTCTGCATCGCCGGCGACGGTTGCTATCTGATGAGCGGCCAGGAGATAACCGTGGCG
GTGGAGCGGCGCCTGCCGGTGATCTTCGCGGTGCTGAACGACCAGGCCTACGGCCTGATCAGGCACGGCCACCGGACCCA
CGGCAGGGAGGCGGTGGATTTCAGCATTCCGCCGGTGGATTTCGCCATGATGGCCCGCTCCACCGGCGCCCAGGCCTTCA
CCATCCGTTCCATCGAGGACTTCGAAACCATCGACTGGCAGAAGCTGGCAACCCACCAGGGGCCGACGCTCTTGGATGTG
ACCATCGACCCGAAGGAACCGCCGCCACTGGCCATGGCGTAG

Upstream 100 bases:

>100_bases
AATGTGCTACCATGCCGCAGCATCCCTTCCGTCATGATTTGTGCGTCGGAAGGATGGAAACGTCGGAAAACGCATTTGAG
CTGGCAAGGAGAACTAAGTA

Downstream 100 bases:

>100_bases
CTGTCGGTTATCAAGATGAGGGCAAAAAACAGCCCTTGATGAGGATTTTCAACGTTCATCAAGGGCTGTCCCGGCAACCC
ACGCCACAATATATACAAGC

Product: thiamine pyrophosphate binding domain-containing protein

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MALTVSDLIVEYLEQFGVEYVFGVPGSPLGPLFDALVCSEKRGGPRLVLARHEAGAAFMADGYARESGRIGVCCSTTGPG
ATNLITGVAGAYAEQVPMLVLSSQTRVTDFSFGCFQDSSRDGVDIMSMFACCTRYNSLVSHPNQLEKKLASALTTALGSP
RGPAHLSIPIDIFGADASGPASYPGLHSLVSAEESTVDGAALERLMNEITTVRSTSGRIVLLAGANAISAGDLITRFAEL
TGAKIITTPRGKPAINPYHPLARGVFGVSGHSSARQTLAEDNVQLILAVGSNLGEWETSKWDPILMNDCMVHIDSDRQNF
TRSPMARLHLYGGIHAIFEQLNRRLEQHDGSAVSRSKGSAETAPATSAYVPAGISVRNADCCAPPASGQLLKAPQVYVEL
IQRLPRESRFFIDNSNSVPWSIHYFFHQRPEALHYSIEFATMAWAVGAAVGGAFANQKAPSVCIAGDGCYLMSGQEITVA
VERRLPVIFAVLNDQAYGLIRHGHRTHGREAVDFSIPPVDFAMMARSTGAQAFTIRSIEDFETIDWQKLATHQGPTLLDV
TIDPKEPPPLAMA

Sequences:

>Translated_573_residues
MALTVSDLIVEYLEQFGVEYVFGVPGSPLGPLFDALVCSEKRGGPRLVLARHEAGAAFMADGYARESGRIGVCCSTTGPG
ATNLITGVAGAYAEQVPMLVLSSQTRVTDFSFGCFQDSSRDGVDIMSMFACCTRYNSLVSHPNQLEKKLASALTTALGSP
RGPAHLSIPIDIFGADASGPASYPGLHSLVSAEESTVDGAALERLMNEITTVRSTSGRIVLLAGANAISAGDLITRFAEL
TGAKIITTPRGKPAINPYHPLARGVFGVSGHSSARQTLAEDNVQLILAVGSNLGEWETSKWDPILMNDCMVHIDSDRQNF
TRSPMARLHLYGGIHAIFEQLNRRLEQHDGSAVSRSKGSAETAPATSAYVPAGISVRNADCCAPPASGQLLKAPQVYVEL
IQRLPRESRFFIDNSNSVPWSIHYFFHQRPEALHYSIEFATMAWAVGAAVGGAFANQKAPSVCIAGDGCYLMSGQEITVA
VERRLPVIFAVLNDQAYGLIRHGHRTHGREAVDFSIPPVDFAMMARSTGAQAFTIRSIEDFETIDWQKLATHQGPTLLDV
TIDPKEPPPLAMA
>Mature_572_residues
ALTVSDLIVEYLEQFGVEYVFGVPGSPLGPLFDALVCSEKRGGPRLVLARHEAGAAFMADGYARESGRIGVCCSTTGPGA
TNLITGVAGAYAEQVPMLVLSSQTRVTDFSFGCFQDSSRDGVDIMSMFACCTRYNSLVSHPNQLEKKLASALTTALGSPR
GPAHLSIPIDIFGADASGPASYPGLHSLVSAEESTVDGAALERLMNEITTVRSTSGRIVLLAGANAISAGDLITRFAELT
GAKIITTPRGKPAINPYHPLARGVFGVSGHSSARQTLAEDNVQLILAVGSNLGEWETSKWDPILMNDCMVHIDSDRQNFT
RSPMARLHLYGGIHAIFEQLNRRLEQHDGSAVSRSKGSAETAPATSAYVPAGISVRNADCCAPPASGQLLKAPQVYVELI
QRLPRESRFFIDNSNSVPWSIHYFFHQRPEALHYSIEFATMAWAVGAAVGGAFANQKAPSVCIAGDGCYLMSGQEITVAV
ERRLPVIFAVLNDQAYGLIRHGHRTHGREAVDFSIPPVDFAMMARSTGAQAFTIRSIEDFETIDWQKLATHQGPTLLDVT
IDPKEPPPLAMA

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=581, Percent_Identity=20.4819277108434, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI21361361, Length=165, Percent_Identity=32.7272727272727, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1790104, Length=569, Percent_Identity=26.5377855887522, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87081685, Length=572, Percent_Identity=25.8741258741259, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1786717, Length=584, Percent_Identity=24.6575342465753, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1787096, Length=579, Percent_Identity=23.3160621761658, Blast_Score=115, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17542570, Length=167, Percent_Identity=30.5389221556886, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323755, Length=589, Percent_Identity=23.2597623089983, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320123, Length=601, Percent_Identity=23.6272878535774, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=569, Percent_Identity=23.3743409490334, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320816, Length=604, Percent_Identity=20.6953642384106, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321524, Length=571, Percent_Identity=21.5411558669002, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=565, Percent_Identity=23.0088495575221, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922626, Length=584, Percent_Identity=23.1164383561644, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61433; Mature: 61302

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTVSDLIVEYLEQFGVEYVFGVPGSPLGPLFDALVCSEKRGGPRLVLARHEAGAAFMA
CEEEHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
DGYARESGRIGVCCSTTGPGATNLITGVAGAYAEQVPMLVLSSQTRVTDFSFGCFQDSSR
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCCCCC
DGVDIMSMFACCTRYNSLVSHPNQLEKKLASALTTALGSPRGPAHLSIPIDIFGADASGP
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCC
ASYPGLHSLVSAEESTVDGAALERLMNEITTVRSTSGRIVLLAGANAISAGDLITRFAEL
CCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
TGAKIITTPRGKPAINPYHPLARGVFGVSGHSSARQTLAEDNVQLILAVGSNLGEWETSK
CCCEEEECCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCC
WDPILMNDCMVHIDSDRQNFTRSPMARLHLYGGIHAIFEQLNRRLEQHDGSAVSRSKGSA
CCCEEECCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCC
ETAPATSAYVPAGISVRNADCCAPPASGQLLKAPQVYVELIQRLPRESRFFIDNSNSVPW
CCCCCCCCCCCCCCEECCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCEEEEECCCCCCE
SIHYFFHQRPEALHYSIEFATMAWAVGAAVGGAFANQKAPSVCIAGDGCYLMSGQEITVA
EEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCEEEEE
VERRLPVIFAVLNDQAYGLIRHGHRTHGREAVDFSIPPVDFAMMARSTGAQAFTIRSIED
EECCCCEEEEEECCCCEEHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCCCEEEEECCCC
FETIDWQKLATHQGPTLLDVTIDPKEPPPLAMA
CCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
ALTVSDLIVEYLEQFGVEYVFGVPGSPLGPLFDALVCSEKRGGPRLVLARHEAGAAFMA
EEEHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
DGYARESGRIGVCCSTTGPGATNLITGVAGAYAEQVPMLVLSSQTRVTDFSFGCFQDSSR
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCCCCC
DGVDIMSMFACCTRYNSLVSHPNQLEKKLASALTTALGSPRGPAHLSIPIDIFGADASGP
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCC
ASYPGLHSLVSAEESTVDGAALERLMNEITTVRSTSGRIVLLAGANAISAGDLITRFAEL
CCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
TGAKIITTPRGKPAINPYHPLARGVFGVSGHSSARQTLAEDNVQLILAVGSNLGEWETSK
CCCEEEECCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCC
WDPILMNDCMVHIDSDRQNFTRSPMARLHLYGGIHAIFEQLNRRLEQHDGSAVSRSKGSA
CCCEEECCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCC
ETAPATSAYVPAGISVRNADCCAPPASGQLLKAPQVYVELIQRLPRESRFFIDNSNSVPW
CCCCCCCCCCCCCCEECCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCEEEEECCCCCCE
SIHYFFHQRPEALHYSIEFATMAWAVGAAVGGAFANQKAPSVCIAGDGCYLMSGQEITVA
EEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCEEEEE
VERRLPVIFAVLNDQAYGLIRHGHRTHGREAVDFSIPPVDFAMMARSTGAQAFTIRSIED
EECCCCEEEEEECCCCEEHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCCCEEEEECCCC
FETIDWQKLATHQGPTLLDVTIDPKEPPPLAMA
CCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]