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The map label for this gene is ligT [C]

Identifier: 118580933

GI number: 118580933

Start: 2732975

End: 2733535

Strand: Reverse

Name: ligT [C]

Synonym: Ppro_2521

Alternate gene names: 118580933

Gene position: 2733535-2732975 (Counterclockwise)

Preceding gene: 118580934

Following gene: 118580932

Centisome position: 68.2

GC content: 67.2

Gene sequence:

>561_bases
ATGCCGCGCTTGTTCATTGCCATCGATCTGCCCGAAGTCGTGCGGCAGGATCTCTGTCGTCTGAACGTGCCGATCCCCGG
TAGCCGCTGGGTTCCCGCCGATCAGCTGCACCTGACCCTGGCATTCCTGGGGGATGTGGGGGAGGAGAGGGTGGCTGGCC
TGGCCAGGGCGCTTGACCAGGTGAGCGCTGCTCCCTTCCGGTTGCGCTTCGACCGGCTGGGCTGCTTCCCCTCCCGTGCG
CGGCCCCGGGTGCTCTGGGTGGGGCTGAAACCGGAGCCGTTGCTCCTCTCCCTGGCGGACCGGCTGCGTGAGTTCCTCCG
CGACTGTTGCATTCCCCAGGAAGAACGCCCCTTCTCGCCCCATATCACCCTGGCCCGACTCAAGCTGCCGGCGGCCCGGG
AGTGTGCCTCCTTTCTGGATCGGCCGTTGGCGCTGGAGCGGACATCGGTTTCCGCCCAGGAGTTCATCCTCTTCGAGAGC
CTCTTGACTGCCGCAGGCGCCATACATACGCCGCTGAAACGCTTTCCGCTCTCCGGCGGCAGTGGCGGCCCGCCAGCCTG
A

Upstream 100 bases:

>100_bases
TGCTGAAGGACCTGAACGACGCCGTAGCCTAGCCTTTTCGTCGTACATCAAGAGCGACCCCCGGGATTTCCCGGGGGTCG
CCGCGTTTGGGGGTGTTCCC

Downstream 100 bases:

>100_bases
GGCGTTGACAAACCGGCCCGCTGCGGGTAAAAATCAACTCGCTTGTCCCCGTTGGGAACGGATTGCCCTTCAGAACTACC
GGACTTTTTAACGCACCCCG

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MPRLFIAIDLPEVVRQDLCRLNVPIPGSRWVPADQLHLTLAFLGDVGEERVAGLARALDQVSAAPFRLRFDRLGCFPSRA
RPRVLWVGLKPEPLLLSLADRLREFLRDCCIPQEERPFSPHITLARLKLPAARECASFLDRPLALERTSVSAQEFILFES
LLTAAGAIHTPLKRFPLSGGSGGPPA

Sequences:

>Translated_186_residues
MPRLFIAIDLPEVVRQDLCRLNVPIPGSRWVPADQLHLTLAFLGDVGEERVAGLARALDQVSAAPFRLRFDRLGCFPSRA
RPRVLWVGLKPEPLLLSLADRLREFLRDCCIPQEERPFSPHITLARLKLPAARECASFLDRPLALERTSVSAQEFILFES
LLTAAGAIHTPLKRFPLSGGSGGPPA
>Mature_185_residues
PRLFIAIDLPEVVRQDLCRLNVPIPGSRWVPADQLHLTLAFLGDVGEERVAGLARALDQVSAAPFRLRFDRLGCFPSRAR
PRVLWVGLKPEPLLLSLADRLREFLRDCCIPQEERPFSPHITLARLKLPAARECASFLDRPLALERTSVSAQEFILFESL
LTAAGAIHTPLKRFPLSGGSGGPPA

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

Organism=Escherichia coli, GI87081692, Length=181, Percent_Identity=35.9116022099448, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20592; Mature: 20461

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLFIAIDLPEVVRQDLCRLNVPIPGSRWVPADQLHLTLAFLGDVGEERVAGLARALDQ
CCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VSAAPFRLRFDRLGCFPSRARPRVLWVGLKPEPLLLSLADRLREFLRDCCIPQEERPFSP
HCCCCCEEEHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HITLARLKLPAARECASFLDRPLALERTSVSAQEFILFESLLTAAGAIHTPLKRFPLSGG
CEEEEEECCCCHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGGPPA
CCCCCC
>Mature Secondary Structure 
PRLFIAIDLPEVVRQDLCRLNVPIPGSRWVPADQLHLTLAFLGDVGEERVAGLARALDQ
CEEEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VSAAPFRLRFDRLGCFPSRARPRVLWVGLKPEPLLLSLADRLREFLRDCCIPQEERPFSP
HCCCCCEEEHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HITLARLKLPAARECASFLDRPLALERTSVSAQEFILFESLLTAAGAIHTPLKRFPLSGG
CEEEEEECCCCHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGGPPA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]