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The map label for this gene is tgt [H]

Identifier: 118580897

GI number: 118580897

Start: 2691190

End: 2692311

Strand: Direct

Name: tgt [H]

Synonym: Ppro_2484

Alternate gene names: 118580897

Gene position: 2691190-2692311 (Clockwise)

Preceding gene: 118580892

Following gene: 118580900

Centisome position: 67.15

GC content: 60.61

Gene sequence:

>1122_bases
GTGGAATTTAAGCTGATCAAAAAAGACAAACAGAGTTCCGCCCGCCTGGGCGTCGTTACGACGGGTCGGGGAGAGATCCA
GACCCCCATCTTCATGCCGGTGGCCACCCACGGCGCCATGAAACCGCTCACACCGGCCCAGATCACCGAGACCGGCGCCC
AGATCATCCTCAGCAACACCTACCACCTGCACCTGCAACCGGGCGAGGGGCTGGTGAAGAAGGCGGGGGGGCTGCACAAG
TTCATGGCATGGGACAAACCGATTCTCACCGATTCGGGCGGTTTTCAGGTCTTCTCGCTCCCCAACAAGCGCATAACCGA
TGAAGGGGTCTTTTTCCGCCACGAGGTAACCGGCGACGAGATCTACCTGGATCCGGCCTCTGCCACCCGCATCCAGCAGG
ATCTGGGCGCCGACATCATCATGGCCTTCGACGAATGCATCCCCTACCCCTGCGAGCGCCAGTATGCCGAACAGTCCACG
AAAAAGACCATTCGCTGGCTGAAGGAGTGCCAGAGAGCCATGACCGACAACGGCCAGGCCCTGTTCGGTATCGTGCAGGG
GAGTGTGTTCGAGGATCTGCGGGCTTACTGCGCCAGGGAGATGCGCGCCCTGGACCTTCCCGGTTACGCCATCGGCGGGG
TCAGTGTGGGCGAGGGACTGGAGCTCCTGAAAAAGGTGGTCGCATGGACGGCTCCCCACCTGCCTGAGGACAAGCCGCGC
TACCTGATGGGGGTGGGACTGCCGGAGGACATCCTGGAAAGCGTGGAGCGGGGCATCGACATGTTCGACTGCGTGATACC
GACCCGCTATGCCCGCAGTGCCACCCTCTTCACCAGGCGGGGCAAGATCAGACTAACCAACAAGAACTACAAGCGGGACT
TCTTCCCCGTGGACCCCTCGTGCACCTGCTATACCTGCGGCAACTTCACCCGCGCCTACCTGCACCACCTCTTTGTTTCC
AACGAGGTACTCTCCGCGGTGCTTTCCGCCATCCACAACGTCCACTTCTACCTGAACATGATGGCGGAAATCCGCACAGC
CATCGCCGACAACCGTTTCATGGAGTACAAGAGTCGCTTCCTGGCCGAATATCTGGAGGGAAACCGGCGCACCAAGCAGT
AA

Upstream 100 bases:

>100_bases
ACTCTGATCCTGTACGGTTGTTTGCTGAAAATCAAATAATTAACAAAAGCTACTCAACAAAAACAGTGCGCCAGATTGGC
GCGTTACCGGAGCAATGCAC

Downstream 100 bases:

>100_bases
CCTCCCCTGCGCGGTTCCAGGTTCACGCCTCATGGCGCACCCTGTTCAGCGCTTGATCTTCTGGTCCAGTTCAATGTCCA
GGAGTTTCAGGCGTTCGATG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MEFKLIKKDKQSSARLGVVTTGRGEIQTPIFMPVATHGAMKPLTPAQITETGAQIILSNTYHLHLQPGEGLVKKAGGLHK
FMAWDKPILTDSGGFQVFSLPNKRITDEGVFFRHEVTGDEIYLDPASATRIQQDLGADIIMAFDECIPYPCERQYAEQST
KKTIRWLKECQRAMTDNGQALFGIVQGSVFEDLRAYCAREMRALDLPGYAIGGVSVGEGLELLKKVVAWTAPHLPEDKPR
YLMGVGLPEDILESVERGIDMFDCVIPTRYARSATLFTRRGKIRLTNKNYKRDFFPVDPSCTCYTCGNFTRAYLHHLFVS
NEVLSAVLSAIHNVHFYLNMMAEIRTAIADNRFMEYKSRFLAEYLEGNRRTKQ

Sequences:

>Translated_373_residues
MEFKLIKKDKQSSARLGVVTTGRGEIQTPIFMPVATHGAMKPLTPAQITETGAQIILSNTYHLHLQPGEGLVKKAGGLHK
FMAWDKPILTDSGGFQVFSLPNKRITDEGVFFRHEVTGDEIYLDPASATRIQQDLGADIIMAFDECIPYPCERQYAEQST
KKTIRWLKECQRAMTDNGQALFGIVQGSVFEDLRAYCAREMRALDLPGYAIGGVSVGEGLELLKKVVAWTAPHLPEDKPR
YLMGVGLPEDILESVERGIDMFDCVIPTRYARSATLFTRRGKIRLTNKNYKRDFFPVDPSCTCYTCGNFTRAYLHHLFVS
NEVLSAVLSAIHNVHFYLNMMAEIRTAIADNRFMEYKSRFLAEYLEGNRRTKQ
>Mature_373_residues
MEFKLIKKDKQSSARLGVVTTGRGEIQTPIFMPVATHGAMKPLTPAQITETGAQIILSNTYHLHLQPGEGLVKKAGGLHK
FMAWDKPILTDSGGFQVFSLPNKRITDEGVFFRHEVTGDEIYLDPASATRIQQDLGADIIMAFDECIPYPCERQYAEQST
KKTIRWLKECQRAMTDNGQALFGIVQGSVFEDLRAYCAREMRALDLPGYAIGGVSVGEGLELLKKVVAWTAPHLPEDKPR
YLMGVGLPEDILESVERGIDMFDCVIPTRYARSATLFTRRGKIRLTNKNYKRDFFPVDPSCTCYTCGNFTRAYLHHLFVS
NEVLSAVLSAIHNVHFYLNMMAEIRTAIADNRFMEYKSRFLAEYLEGNRRTKQ

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=362, Percent_Identity=42.2651933701657, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI13375872, Length=355, Percent_Identity=25.3521126760563, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1786607, Length=363, Percent_Identity=49.3112947658402, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17544680, Length=353, Percent_Identity=43.6260623229462, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI25146502, Length=173, Percent_Identity=28.9017341040462, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920510, Length=369, Percent_Identity=41.1924119241192, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI21355311, Length=174, Percent_Identity=33.9080459770115, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42106; Mature: 42106

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKLIKKDKQSSARLGVVTTGRGEIQTPIFMPVATHGAMKPLTPAQITETGAQIILSNT
CCCEECCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCEEEEECE
YHLHLQPGEGLVKKAGGLHKFMAWDKPILTDSGGFQVFSLPNKRITDEGVFFRHEVTGDE
EEEEEECCCCHHHHCCCHHHHHHCCCCEEECCCCEEEEECCCCCCCCCCCEEEEECCCCE
IYLDPASATRIQQDLGADIIMAFDECIPYPCERQYAEQSTKKTIRWLKECQRAMTDNGQA
EEECCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LFGIVQGSVFEDLRAYCAREMRALDLPGYAIGGVSVGEGLELLKKVVAWTAPHLPEDKPR
EEEEHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCC
YLMGVGLPEDILESVERGIDMFDCVIPTRYARSATLFTRRGKIRLTNKNYKRDFFPVDPS
EEEECCCCHHHHHHHHHCCCHHHHHCCCHHHCCCEEEEECCEEEECCCCCCCCCCCCCCC
CTCYTCGNFTRAYLHHLFVSNEVLSAVLSAIHNVHFYLNMMAEIRTAIADNRFMEYKSRF
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEYLEGNRRTKQ
HHHHHCCCCCCCC
>Mature Secondary Structure
MEFKLIKKDKQSSARLGVVTTGRGEIQTPIFMPVATHGAMKPLTPAQITETGAQIILSNT
CCCEECCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCEEEEECE
YHLHLQPGEGLVKKAGGLHKFMAWDKPILTDSGGFQVFSLPNKRITDEGVFFRHEVTGDE
EEEEEECCCCHHHHCCCHHHHHHCCCCEEECCCCEEEEECCCCCCCCCCCEEEEECCCCE
IYLDPASATRIQQDLGADIIMAFDECIPYPCERQYAEQSTKKTIRWLKECQRAMTDNGQA
EEECCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LFGIVQGSVFEDLRAYCAREMRALDLPGYAIGGVSVGEGLELLKKVVAWTAPHLPEDKPR
EEEEHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCC
YLMGVGLPEDILESVERGIDMFDCVIPTRYARSATLFTRRGKIRLTNKNYKRDFFPVDPS
EEEECCCCHHHHHHHHHCCCHHHHHCCCHHHCCCEEEEECCEEEECCCCCCCCCCCCCCC
CTCYTCGNFTRAYLHHLFVSNEVLSAVLSAIHNVHFYLNMMAEIRTAIADNRFMEYKSRF
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEYLEGNRRTKQ
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA