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The map label for this gene is asnB [H]

Identifier: 118580879

GI number: 118580879

Start: 2667720

End: 2669597

Strand: Reverse

Name: asnB [H]

Synonym: Ppro_2466

Alternate gene names: 118580879

Gene position: 2669597-2667720 (Counterclockwise)

Preceding gene: 118580880

Following gene: 118580878

Centisome position: 66.61

GC content: 60.17

Gene sequence:

>1878_bases
ATGTGCGGCATAGCCGGCTTCTTCAGATCCTGCGCCCCTGGTGCCGATGAATCGGTACTTCGCCGGATGGGTGAGGCGAT
ACGTCACCGCGGGCCGGATGCCGGTGGCGAGTACCTGGACGAACATGTCGGACTGGCCCACCGTCGCCTGAGCATCATCG
ACCTCTCCAGCGATGGCAACCAGCCGATGTTTTTCGGGGATGGTCGCTACGTGATCGTCTTCAACGGAGAAATCTATAAC
TATCGCGAACTGAAAGATGAACTGGAGAGAGCGGGAACCGTCTTCAATTCCCGCAGCGACACCGAAGTGATCTTGGCCCT
GTACGCGGCAAAGGGGCGCGAATGCTTGAACGACCTGAACGGCATGTTCGCCTTCGCCCTGTGGGACAGAATGGAGAAGA
CCCTCTTTCTGGCCCGTGACCGGATCGGCAAGAAGCCGCTTTACTACTACCATGCAGGCGGCGACCGCCTGGCATTCGCC
TCGGAGATCAAGGCGCTCCTGCAGGTTCCCGGGATCTCGCGGGAGATTGATCCGACCGCGGTGGTGGATTACCTCAAGTA
CCTGTATGTGCCGGCTCCGAAATCGATTTTTCGCAATATCCACAAACTCCCGGCTGGGCACTGGCTGGAACTGCGGGTGG
GGAGTGAACCGAGGATCGGCCAGTACTGGGATGTGGACTTCTCGCGCACGAGAGGCGGAAGCATCGAGGATGCGGCCGAT
GAGCTGCTGTACCTCATGCGGGATGCCACCCGTTGCCGCATGATCTCCGACGTTCCCTTGGGTGCCTTTCTCAGTGGCGG
CATCGATTCCAGCGCCGTGGTTGCCCTGATGGCTGGCGTCTCCAACGAAAAGGTGAGGACCTGCTCCATCGGCTTTTCTG
ACCGGACACATGACGAAACTCCCTATGCGCGCGAGGTTGCGGCCCTGTTCGGCACCGATCACCGGGAGTACATGGTTCGG
GAAAACCTGCCGGAAACAGTGCTGCGTCAGGCGCGCTTTTTCGACGAACCCTTTGCCGATTCCTCCTCCGTCCCCACCTA
TCATGTCTCCAGGCTGGCCCGCCAGGACGTCACGGTGGCCCTGGCCGGCGATGGCGGTGACGAGAGCTTCGGCGGCTACG
AGAAATACACTATCGACCGGCGCGAGCACCAGGCGCGTCAACTGGTTCCGCGTCCAATTCTGAGGTTTCTGCGGGGGGTT
GCCCAGGGTCAGCCGGGAGTGGTGGCCCGCAAGGTCCGCACACTGACCGGAGGCGCCCTCTCCGATCCGGCCGTGGCTTT
CTACCGTACCAATACCTTCATAGAGGACGATCTTCTCAATGAACTGCTGGGTGAACGCCTCAGGCGCGGAACCAGCGGCT
ATGATCCGTCGCGGCACACCGTGGATGCCTGGGATAAGATGCGGGGGGCGGATCATGTTGCCTGCATGCTCTACACTGAT
CTCAAAACCTATCTTCCCGATGACATCCTGGTAAAGGTTGACCGTATGAGCATGGCCCATTCCCTGGAGGTCCGGGCTCC
GCTTCTGGATTACCGTGTCATCGAGTTCGCTGCCTCCCTGCCCAGTTCCTGGAAGATCCGCGGTGCCACCAAGAAATACC
TGTTGAAGCAATCCTTCCGTAAGGTGCTTCCCGATTCCATCCTCCACCGGCGCAAGCATGGTTTCACGGTTCCGCTGGGC
GACTGGTTCCGTCGGGACTTGCGTGGGGTGGCCGAGGAACATCTGCTGGGGCCCGGGATGGAGGAGTATTTCTCGCCGGC
CTGCCTGCGGCGCCTCTGGCAAGAGCACCAGGATAAGAAGATGGATCATGGCACACTGCTCTGGACCCTGTTTTCCTTTT
CTCTCTGGCGCATGAATTATCTGGGGACCAGCGGATGA

Upstream 100 bases:

>100_bases
CAGTGTCGCAACTGTACGTGCTGTTCAACATCTATTTTCTCGCCCGCGGGTTGGCGAGCCTGCGCTGGAGATAACTGATG
TCCGGAAAGGTTGAATCCAT

Downstream 100 bases:

>100_bases
TGACCACGACCAGGCACATCCTCGTCCGAGAGACCGCGGGCATTTCCCGCTGTGGTGAGATCGTCAGGCTGGGCGTTCCC
TGTGCCCGTGGCGAATTCCC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MCGIAGFFRSCAPGADESVLRRMGEAIRHRGPDAGGEYLDEHVGLAHRRLSIIDLSSDGNQPMFFGDGRYVIVFNGEIYN
YRELKDELERAGTVFNSRSDTEVILALYAAKGRECLNDLNGMFAFALWDRMEKTLFLARDRIGKKPLYYYHAGGDRLAFA
SEIKALLQVPGISREIDPTAVVDYLKYLYVPAPKSIFRNIHKLPAGHWLELRVGSEPRIGQYWDVDFSRTRGGSIEDAAD
ELLYLMRDATRCRMISDVPLGAFLSGGIDSSAVVALMAGVSNEKVRTCSIGFSDRTHDETPYAREVAALFGTDHREYMVR
ENLPETVLRQARFFDEPFADSSSVPTYHVSRLARQDVTVALAGDGGDESFGGYEKYTIDRREHQARQLVPRPILRFLRGV
AQGQPGVVARKVRTLTGGALSDPAVAFYRTNTFIEDDLLNELLGERLRRGTSGYDPSRHTVDAWDKMRGADHVACMLYTD
LKTYLPDDILVKVDRMSMAHSLEVRAPLLDYRVIEFAASLPSSWKIRGATKKYLLKQSFRKVLPDSILHRRKHGFTVPLG
DWFRRDLRGVAEEHLLGPGMEEYFSPACLRRLWQEHQDKKMDHGTLLWTLFSFSLWRMNYLGTSG

Sequences:

>Translated_625_residues
MCGIAGFFRSCAPGADESVLRRMGEAIRHRGPDAGGEYLDEHVGLAHRRLSIIDLSSDGNQPMFFGDGRYVIVFNGEIYN
YRELKDELERAGTVFNSRSDTEVILALYAAKGRECLNDLNGMFAFALWDRMEKTLFLARDRIGKKPLYYYHAGGDRLAFA
SEIKALLQVPGISREIDPTAVVDYLKYLYVPAPKSIFRNIHKLPAGHWLELRVGSEPRIGQYWDVDFSRTRGGSIEDAAD
ELLYLMRDATRCRMISDVPLGAFLSGGIDSSAVVALMAGVSNEKVRTCSIGFSDRTHDETPYAREVAALFGTDHREYMVR
ENLPETVLRQARFFDEPFADSSSVPTYHVSRLARQDVTVALAGDGGDESFGGYEKYTIDRREHQARQLVPRPILRFLRGV
AQGQPGVVARKVRTLTGGALSDPAVAFYRTNTFIEDDLLNELLGERLRRGTSGYDPSRHTVDAWDKMRGADHVACMLYTD
LKTYLPDDILVKVDRMSMAHSLEVRAPLLDYRVIEFAASLPSSWKIRGATKKYLLKQSFRKVLPDSILHRRKHGFTVPLG
DWFRRDLRGVAEEHLLGPGMEEYFSPACLRRLWQEHQDKKMDHGTLLWTLFSFSLWRMNYLGTSG
>Mature_625_residues
MCGIAGFFRSCAPGADESVLRRMGEAIRHRGPDAGGEYLDEHVGLAHRRLSIIDLSSDGNQPMFFGDGRYVIVFNGEIYN
YRELKDELERAGTVFNSRSDTEVILALYAAKGRECLNDLNGMFAFALWDRMEKTLFLARDRIGKKPLYYYHAGGDRLAFA
SEIKALLQVPGISREIDPTAVVDYLKYLYVPAPKSIFRNIHKLPAGHWLELRVGSEPRIGQYWDVDFSRTRGGSIEDAAD
ELLYLMRDATRCRMISDVPLGAFLSGGIDSSAVVALMAGVSNEKVRTCSIGFSDRTHDETPYAREVAALFGTDHREYMVR
ENLPETVLRQARFFDEPFADSSSVPTYHVSRLARQDVTVALAGDGGDESFGGYEKYTIDRREHQARQLVPRPILRFLRGV
AQGQPGVVARKVRTLTGGALSDPAVAFYRTNTFIEDDLLNELLGERLRRGTSGYDPSRHTVDAWDKMRGADHVACMLYTD
LKTYLPDDILVKVDRMSMAHSLEVRAPLLDYRVIEFAASLPSSWKIRGATKKYLLKQSFRKVLPDSILHRRKHGFTVPLG
DWFRRDLRGVAEEHLLGPGMEEYFSPACLRRLWQEHQDKKMDHGTLLWTLFSFSLWRMNYLGTSG

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=411, Percent_Identity=27.9805352798054, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI168229252, Length=411, Percent_Identity=27.9805352798054, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI168229250, Length=411, Percent_Identity=27.9805352798054, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI296010848, Length=383, Percent_Identity=28.1984334203655, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786889, Length=397, Percent_Identity=32.9974811083123, Blast_Score=161, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI71993933, Length=411, Percent_Identity=30.6569343065693, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25147557, Length=411, Percent_Identity=30.9002433090024, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25147560, Length=329, Percent_Identity=31.0030395136778, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560178, Length=390, Percent_Identity=25.1282051282051, Blast_Score=99, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=412, Percent_Identity=28.8834951456311, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6325403, Length=413, Percent_Identity=28.3292978208232, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45553209, Length=397, Percent_Identity=31.2342569269521, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70737; Mature: 70737

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGFFRSCAPGADESVLRRMGEAIRHRGPDAGGEYLDEHVGLAHRRLSIIDLSSDGN
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEEEECCCCC
QPMFFGDGRYVIVFNGEIYNYRELKDELERAGTVFNSRSDTEVILALYAAKGRECLNDLN
CCEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHH
GMFAFALWDRMEKTLFLARDRIGKKPLYYYHAGGDRLAFASEIKALLQVPGISREIDPTA
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHH
VVDYLKYLYVPAPKSIFRNIHKLPAGHWLELRVGSEPRIGQYWDVDFSRTRGGSIEDAAD
HHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCCCCCCCCCHHHHH
ELLYLMRDATRCRMISDVPLGAFLSGGIDSSAVVALMAGVSNEKVRTCSIGFSDRTHDET
HHHHHHHHHHHHHEECCCCHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCC
PYAREVAALFGTDHREYMVRENLPETVLRQARFFDEPFADSSSVPTYHVSRLARQDVTVA
CHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEEE
LAGDGGDESFGGYEKYTIDRREHQARQLVPRPILRFLRGVAQGQPGVVARKVRTLTGGAL
EECCCCCCCCCCCHHEECCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
SDPAVAFYRTNTFIEDDLLNELLGERLRRGTSGYDPSRHTVDAWDKMRGADHVACMLYTD
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEHH
LKTYLPDDILVKVDRMSMAHSLEVRAPLLDYRVIEFAASLPSSWKIRGATKKYLLKQSFR
HHHHCCCHHEEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHH
KVLPDSILHRRKHGFTVPLGDWFRRDLRGVAEEHLLGPGMEEYFSPACLRRLWQEHQDKK
HHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHCC
MDHGTLLWTLFSFSLWRMNYLGTSG
CCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCGIAGFFRSCAPGADESVLRRMGEAIRHRGPDAGGEYLDEHVGLAHRRLSIIDLSSDGN
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEEEEECCCCC
QPMFFGDGRYVIVFNGEIYNYRELKDELERAGTVFNSRSDTEVILALYAAKGRECLNDLN
CCEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHH
GMFAFALWDRMEKTLFLARDRIGKKPLYYYHAGGDRLAFASEIKALLQVPGISREIDPTA
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHH
VVDYLKYLYVPAPKSIFRNIHKLPAGHWLELRVGSEPRIGQYWDVDFSRTRGGSIEDAAD
HHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCCCCCCCCCHHHHH
ELLYLMRDATRCRMISDVPLGAFLSGGIDSSAVVALMAGVSNEKVRTCSIGFSDRTHDET
HHHHHHHHHHHHHEECCCCHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCC
PYAREVAALFGTDHREYMVRENLPETVLRQARFFDEPFADSSSVPTYHVSRLARQDVTVA
CHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEEE
LAGDGGDESFGGYEKYTIDRREHQARQLVPRPILRFLRGVAQGQPGVVARKVRTLTGGAL
EECCCCCCCCCCCHHEECCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
SDPAVAFYRTNTFIEDDLLNELLGERLRRGTSGYDPSRHTVDAWDKMRGADHVACMLYTD
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEHH
LKTYLPDDILVKVDRMSMAHSLEVRAPLLDYRVIEFAASLPSSWKIRGATKKYLLKQSFR
HHHHCCCHHEEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHH
KVLPDSILHRRKHGFTVPLGDWFRRDLRGVAEEHLLGPGMEEYFSPACLRRLWQEHQDKK
HHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHCC
MDHGTLLWTLFSFSLWRMNYLGTSG
CCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]