Click here to switch to the map view.

The map label for this gene is lepA

Identifier: 118580746

GI number: 118580746

Start: 2517014

End: 2518810

Strand: Reverse

Name: lepA

Synonym: Ppro_2331

Alternate gene names: 118580746

Gene position: 2518810-2517014 (Counterclockwise)

Preceding gene: 118580747

Following gene: 118580745

Centisome position: 62.84

GC content: 61.55

Gene sequence:

>1797_bases
ATGGACATCAGCAGACTCCGTAATTTTTCCATCATTGCCCACATTGACCACGGCAAATCAACGCTGGCCGACCGACTGCT
GGAGTTTACCGGCGCCCTTTCCCAGCGGGAGATGCAGAACCAGTTCCTCGACAAGATGGACCTGGAGCGTGAACGGGGCA
TCACCATCAAGGCCCAGACCGTGCGGCTCACCTATCGCGCCGATGACGGCAACGACTATGTGCTCAACCTGATCGACACC
CCCGGTCACGTGGACTTCACCTACGAGGTATCCCGCTCCCTGGCCGCCTGCGAGGGGGGGCTCTTGGTGGTTGACGCCTC
CCAGGGGGTGGAGGCCCAGACCCTGGCCAACGTCTACCTGGCCCTGGACATCAACCTGGAGGTCTTTCCGGTCCTGAACA
AGATCGATCTTCCGGCCGCTGACCCGGAGCGGGTCATTCAGGAGATCGAGGAGATCATCGGCATCGATGCCCATGACGCC
GTCCTGGCCAGCGCCAAGGAGGGGATCGGCACCCACGAGATCCTGGAGGAGATCGTCAAGAAGATCCCGCCCCCTAAAGG
CGATGCGTCGGCTCCGCTAAGAGCGCTTCTGTTCGACTCCTGGTACGACCAGTACCAGGGGGTGATCATCCTGGTGCGCA
TCTTCGACGGCACCCTCAAAAAGGGGGACCGCATCCAGCTCATGGCCACCAGGAGCTCCTTCGAGGCGCTCAAGGTGGGC
GTGTTCGCCCCCACCATGGTGGAGACTCAGCAGTTGGCTGCCGGCGAGGTCGGTTTCGTCATCGCCGGCATCAAGGAAGT
GGCCGACGCCAAGGTGGGTGATACGGTCACCCTGCAGCACCGCCCCTGTTCCTCCGCCCTGGAGGGGTTCAAGGAGGTTA
AGCCGATGGTCTTCTCCGGCCTGTACCCCATCGACACCGTCCAGTACGAGCAGCTGCGTGATGCCCTGGCCAAGCTCAAG
CTGAACGATTCCTCCTTCTCCTATGACCCGGAGACCTCCCTGGCCCTGGGCTTCGGCTTCCGCTGCGGCTTCCTGGGTCT
GTTGCACATGGAGATCATCCAGGAGCGCCTGGAGCGGGAGTTCGGCCTGGACCTGATCACCACCGCGCCCACGGTTGTCT
ACCGCGTCCACCGGGTCACCGGCGAGGTGCAGAGCATCGAGAGTGCCAACCAGATGCCCGAGCTGCAGCATGTGGAGTAC
CTGGAGGAGCCCTTCATCCTGGCCCACATCCATGTGCCCAACGAGTTCGTGGGCGGGGTGCTGGCCCTGTGCGAGGACAA
GCGCGGCGTGCAGCGGGAGATCAAGTACCTGACCCAGAACCGGGTCATGATCATCTACGAGTTGCCGCTGAACGAGATCG
TGCTGGACTTCTACGACCGCCTCAAGTCCATCACCAAGGGGTATGCCTCCCTGGATTACGAGCACCTGGACTACCGCAAG
AGCGACCTAGTGCGCATGAACGTCATGATCAACGGCGAGGTGGTTGATGCCCTCTCCTTAATCCTGCACCGGGACAAGGC
CTACTACCGCGGACGGGACCTGGTCTCCAAGATGAAGGAGCTGATACCGCGCCAGATGTTCGAGGTTGCCATCCAGGCGG
CCATCGGCAACAAGATCATCGCCCGCGAAACGGTCAAGGCCATGCGCAAGGACGTGCTGGCCAAGTGCTATGGCGGCGAC
ATCACCCGCAAGCGCAAGCTTTTGGAGAAGCAGAAGGAAGGCAAGAAGCGCATGAAGAACGTGGGCAACGTGGAACTGCC
CCAGGACGCCTTTCTGGCGATTCTCAAAGTGGAGTAG

Upstream 100 bases:

>100_bases
GCCACGGTGGCCAGCCCCTTTTCCTCTTTACATTCCGGCGCTAATTATTTATTGTTCAACATTACACTTTCGAGCACCCC
AGAGCATATCCAAGGATTTC

Downstream 100 bases:

>100_bases
CGGGTCACAGCCAAAGAAAACAGTCAGGCGATTTGCGCATAGCTCGCGCGCCTCCGCCCCAAGAGGAAGCAACATGCAAG
AAAAAGTGGAAGCAGCTCAG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MDISRLRNFSIIAHIDHGKSTLADRLLEFTGALSQREMQNQFLDKMDLERERGITIKAQTVRLTYRADDGNDYVLNLIDT
PGHVDFTYEVSRSLAACEGGLLVVDASQGVEAQTLANVYLALDINLEVFPVLNKIDLPAADPERVIQEIEEIIGIDAHDA
VLASAKEGIGTHEILEEIVKKIPPPKGDASAPLRALLFDSWYDQYQGVIILVRIFDGTLKKGDRIQLMATRSSFEALKVG
VFAPTMVETQQLAAGEVGFVIAGIKEVADAKVGDTVTLQHRPCSSALEGFKEVKPMVFSGLYPIDTVQYEQLRDALAKLK
LNDSSFSYDPETSLALGFGFRCGFLGLLHMEIIQERLEREFGLDLITTAPTVVYRVHRVTGEVQSIESANQMPELQHVEY
LEEPFILAHIHVPNEFVGGVLALCEDKRGVQREIKYLTQNRVMIIYELPLNEIVLDFYDRLKSITKGYASLDYEHLDYRK
SDLVRMNVMINGEVVDALSLILHRDKAYYRGRDLVSKMKELIPRQMFEVAIQAAIGNKIIARETVKAMRKDVLAKCYGGD
ITRKRKLLEKQKEGKKRMKNVGNVELPQDAFLAILKVE

Sequences:

>Translated_598_residues
MDISRLRNFSIIAHIDHGKSTLADRLLEFTGALSQREMQNQFLDKMDLERERGITIKAQTVRLTYRADDGNDYVLNLIDT
PGHVDFTYEVSRSLAACEGGLLVVDASQGVEAQTLANVYLALDINLEVFPVLNKIDLPAADPERVIQEIEEIIGIDAHDA
VLASAKEGIGTHEILEEIVKKIPPPKGDASAPLRALLFDSWYDQYQGVIILVRIFDGTLKKGDRIQLMATRSSFEALKVG
VFAPTMVETQQLAAGEVGFVIAGIKEVADAKVGDTVTLQHRPCSSALEGFKEVKPMVFSGLYPIDTVQYEQLRDALAKLK
LNDSSFSYDPETSLALGFGFRCGFLGLLHMEIIQERLEREFGLDLITTAPTVVYRVHRVTGEVQSIESANQMPELQHVEY
LEEPFILAHIHVPNEFVGGVLALCEDKRGVQREIKYLTQNRVMIIYELPLNEIVLDFYDRLKSITKGYASLDYEHLDYRK
SDLVRMNVMINGEVVDALSLILHRDKAYYRGRDLVSKMKELIPRQMFEVAIQAAIGNKIIARETVKAMRKDVLAKCYGGD
ITRKRKLLEKQKEGKKRMKNVGNVELPQDAFLAILKVE
>Mature_598_residues
MDISRLRNFSIIAHIDHGKSTLADRLLEFTGALSQREMQNQFLDKMDLERERGITIKAQTVRLTYRADDGNDYVLNLIDT
PGHVDFTYEVSRSLAACEGGLLVVDASQGVEAQTLANVYLALDINLEVFPVLNKIDLPAADPERVIQEIEEIIGIDAHDA
VLASAKEGIGTHEILEEIVKKIPPPKGDASAPLRALLFDSWYDQYQGVIILVRIFDGTLKKGDRIQLMATRSSFEALKVG
VFAPTMVETQQLAAGEVGFVIAGIKEVADAKVGDTVTLQHRPCSSALEGFKEVKPMVFSGLYPIDTVQYEQLRDALAKLK
LNDSSFSYDPETSLALGFGFRCGFLGLLHMEIIQERLEREFGLDLITTAPTVVYRVHRVTGEVQSIESANQMPELQHVEY
LEEPFILAHIHVPNEFVGGVLALCEDKRGVQREIKYLTQNRVMIIYELPLNEIVLDFYDRLKSITKGYASLDYEHLDYRK
SDLVRMNVMINGEVVDALSLILHRDKAYYRGRDLVSKMKELIPRQMFEVAIQAAIGNKIIARETVKAMRKDVLAKCYGGD
ITRKRKLLEKQKEGKKRMKNVGNVELPQDAFLAILKVE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=614, Percent_Identity=52.442996742671, Blast_Score=658, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=42.6470588235294, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI18390331, Length=159, Percent_Identity=35.8490566037736, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI25306283, Length=152, Percent_Identity=42.1052631578947, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI19923640, Length=152, Percent_Identity=42.1052631578947, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI25306287, Length=152, Percent_Identity=42.1052631578947, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=39.3103448275862, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=39.8230088495575, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=39.8230088495575, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=39.8230088495575, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI217272894, Length=176, Percent_Identity=32.9545454545455, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI217272892, Length=176, Percent_Identity=32.9545454545455, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI223555963, Length=274, Percent_Identity=25.9124087591241, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI5729864, Length=274, Percent_Identity=25.9124087591241, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI94966752, Length=96, Percent_Identity=42.7083333333333, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788922, Length=595, Percent_Identity=62.1848739495798, Blast_Score=748, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=510, Percent_Identity=29.4117647058824, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1790835, Length=161, Percent_Identity=31.055900621118, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789738, Length=211, Percent_Identity=32.7014218009479, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789559, Length=258, Percent_Identity=29.0697674418605, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=611, Percent_Identity=42.0621931260229, Blast_Score=494, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17556745, Length=509, Percent_Identity=27.8978388998035, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17533571, Length=166, Percent_Identity=35.5421686746988, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=36.0248447204969, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.8620689655172, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71988811, Length=132, Percent_Identity=34.8484848484849, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71988819, Length=132, Percent_Identity=34.8484848484849, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71994658, Length=153, Percent_Identity=30.0653594771242, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=598, Percent_Identity=47.1571906354515, Blast_Score=561, Evalue=1e-161,
Organism=Saccharomyces cerevisiae, GI6323098, Length=182, Percent_Identity=36.8131868131868, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=40.4109589041096, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=40.4109589041096, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=41.7391304347826, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=40, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324761, Length=284, Percent_Identity=27.4647887323944, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=45.6953642384106, Blast_Score=568, Evalue=1e-162,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=37.2670807453416, Blast_Score=109, Evalue=7e-24,
Organism=Drosophila melanogaster, GI28574573, Length=255, Percent_Identity=34.9019607843137, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=41.0596026490066, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=35.9477124183007, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=35.9477124183007, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=35.9477124183007, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357743, Length=160, Percent_Identity=32.5, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281363316, Length=281, Percent_Identity=26.3345195729537, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17864358, Length=281, Percent_Identity=26.3345195729537, Blast_Score=70, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_PELPD (A1ARG8)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_901996.1
- ProteinModelPortal:   A1ARG8
- SMR:   A1ARG8
- STRING:   A1ARG8
- GeneID:   4571648
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_2331
- eggNOG:   COG0481
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- PhylomeDB:   A1ARG8
- ProtClustDB:   PRK05433
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 67227; Mature: 67227

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISRLRNFSIIAHIDHGKSTLADRLLEFTGALSQREMQNQFLDKMDLERERGITIKAQT
CCHHHCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCEEEEEE
VRLTYRADDGNDYVLNLIDTPGHVDFTYEVSRSLAACEGGLLVVDASQGVEAQTLANVYL
EEEEEEECCCCCEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHEEEEE
ALDINLEVFPVLNKIDLPAADPERVIQEIEEIIGIDAHDAVLASAKEGIGTHEILEEIVK
EEEECEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHH
KIPPPKGDASAPLRALLFDSWYDQYQGVIILVRIFDGTLKKGDRIQLMATRSSFEALKVG
HCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCHHHHEEE
VFAPTMVETQQLAAGEVGFVIAGIKEVADAKVGDTVTLQHRPCSSALEGFKEVKPMVFSG
ECCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCHHHHCC
LYPIDTVQYEQLRDALAKLKLNDSSFSYDPETSLALGFGFRCGFLGLLHMEIIQERLERE
CCCCCCCCHHHHHHHHHHEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
FGLDLITTAPTVVYRVHRVTGEVQSIESANQMPELQHVEYLEEPFILAHIHVPNEFVGGV
HCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEEECCHHHHHHH
LALCEDKRGVQREIKYLTQNRVMIIYELPLNEIVLDFYDRLKSITKGYASLDYEHLDYRK
HHHHHCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
SDLVRMNVMINGEVVDALSLILHRDKAYYRGRDLVSKMKELIPRQMFEVAIQAAIGNKII
CCEEEEEEEECCHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARETVKAMRKDVLAKCYGGDITRKRKLLEKQKEGKKRMKNVGNVELPQDAFLAILKVE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEC
>Mature Secondary Structure
MDISRLRNFSIIAHIDHGKSTLADRLLEFTGALSQREMQNQFLDKMDLERERGITIKAQT
CCHHHCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCEEEEEE
VRLTYRADDGNDYVLNLIDTPGHVDFTYEVSRSLAACEGGLLVVDASQGVEAQTLANVYL
EEEEEEECCCCCEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHEEEEE
ALDINLEVFPVLNKIDLPAADPERVIQEIEEIIGIDAHDAVLASAKEGIGTHEILEEIVK
EEEECEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHH
KIPPPKGDASAPLRALLFDSWYDQYQGVIILVRIFDGTLKKGDRIQLMATRSSFEALKVG
HCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCHHHHEEE
VFAPTMVETQQLAAGEVGFVIAGIKEVADAKVGDTVTLQHRPCSSALEGFKEVKPMVFSG
ECCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCHHHHCC
LYPIDTVQYEQLRDALAKLKLNDSSFSYDPETSLALGFGFRCGFLGLLHMEIIQERLERE
CCCCCCCCHHHHHHHHHHEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
FGLDLITTAPTVVYRVHRVTGEVQSIESANQMPELQHVEYLEEPFILAHIHVPNEFVGGV
HCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEEECCHHHHHHH
LALCEDKRGVQREIKYLTQNRVMIIYELPLNEIVLDFYDRLKSITKGYASLDYEHLDYRK
HHHHHCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
SDLVRMNVMINGEVVDALSLILHRDKAYYRGRDLVSKMKELIPRQMFEVAIQAAIGNKII
CCEEEEEEEECCHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARETVKAMRKDVLAKCYGGDITRKRKLLEKQKEGKKRMKNVGNVELPQDAFLAILKVE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA