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The map label for this gene is tilS [H]

Identifier: 118580663

GI number: 118580663

Start: 2426817

End: 2428274

Strand: Direct

Name: tilS [H]

Synonym: Ppro_2248

Alternate gene names: 118580663

Gene position: 2426817-2428274 (Clockwise)

Preceding gene: 118580662

Following gene: 118580664

Centisome position: 60.55

GC content: 67.15

Gene sequence:

>1458_bases
GTGATCGCCACGAACCTCCCCGCACGCGTCAGCCGCACCATCCGTGATCAGCAACTCTTCGCCCCCGGCGACACCGTCAT
CCTGGCTCTGTCCGGAGGGGCGGACTCCTGCGTTCTGCTGGACATCCTGGCCGGGCTGACGGAGTTCTCCCCCTGCCTGG
TGATAGCCCACCTCAACCACCAGCTGCGGGGGGCTGAATCCGACGGCGACGAGTTATTCTGCCGCAGCCTGGCCGAACGC
TACGGCCTCCCCTTCCGCTCCCGCAGCCTGGACGTGGCCGCCCTGGCCCGCCGGCAGGGGCTCAACCTGGAGGATGCCGG
ACGCCGGGCTCGTCTGGAATTCCTGGAGGACGTTGCCAGGAGTTTTCATGCAACGGCCATCGCCCTGGCCCACCACGCCG
ACGACCAGGCAGAAACCGTGCTGATGCGCCTCTTGCGCGGATCGGGCATGACCGGTCTGCGCGGCATGTCCTTCCGCGGC
CCGGGCGGCCGCATCCGCCCCCTGCTGAACAGCCGCCGCTGCGAGATCGATGCCTATCTGCGGGCACGGGGGCTGGAGCA
CCGCGAGGATGCCAGCAACCGCGACACCGCCTTCCTGCGAAACCGCATCCGCCACGAACTGCTGCCGCTCTTGGAACAGT
ACAACCCCGCCATCCGCGAGCGCCTGGTCGGCACCGCCGCGCTCCTGGCCGACGAGGACGACCTGCTGGAGGGGATGGCG
GAGGATCTGGCGGCCAGGGCCTGCCATTTCGGCAGCGACAGCGTCTCCTGCTCCCTGACCCGACTGGAGGGGGAACCCCA
GGCACTCAAACGCAGGCTGGTGCGCCGGATCCTGGCCCATCTGACAGGGAACGGCGAACACGTTACCCGGCGCCATATCC
AGGCCATGGAAGATCTCATGGATTCTCCCCGGCCCAACGCGACCCTTAACCTGTTCCGAGGGATCAGGGCGGTACGGGAA
TACGGAACCTTGCGCCTCTCAAGGGGGCTGCCGCCGGAGGATCTCCCCCATGAAGCGCGGGAACTGCCCGGCCCCGGCCG
ATACATTCTCTCCGACGGTTCCCTGCTGACGCTGGCTCCGGCGACCGCTCCGCCGGATCCGGCCTCCCTGCCGGCAACCG
TGGCCCTCTTCGACCTGGAGAAAGCCCCCTTTCCCTGGAGGGTGCGCACCTTCACCCCCGGAGACCGGATCGCCCCCTTT
GCCATGGCCGGCAGCAAGAAGGTCAAGGAACTGTTCATGGAACGCAGGGTCCCCCGCGACCAACGGGGACGGATCCCGCT
AATCTTCTGTGGCGACGACCTGATCTGGGTCTGCGGCCTGCGCACATCCCGACTGGCGCAGCTTGACGCCGGATCCCGGA
ACATCATTATGGCGGTATACTCTCCACGGTCGGGGGATGATCGACTGACCGACGCCAAGGAGAGGGACAGGGAGGAAAAA
CCATGCGCATGGGAATGA

Upstream 100 bases:

>100_bases
TGAAGGCGTCGGCCGAAGAGAACGCCAGGATCATGCTCCAGTCCATGCAGGATGCCATGCAGCAGGTCATGGCCGAAGTA
AGCGCCCTTTTCGACAGAAA

Downstream 100 bases:

>100_bases
TCGGAGTGGGGAGGATGGGCGGAGACATGGTGCGGAGGCTCTTGAAGGGCGGTCACGAGTGCGTGGTCCACGCCCGCAGA
CCATCGTCCATGGAACCGCT

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MIATNLPARVSRTIRDQQLFAPGDTVILALSGGADSCVLLDILAGLTEFSPCLVIAHLNHQLRGAESDGDELFCRSLAER
YGLPFRSRSLDVAALARRQGLNLEDAGRRARLEFLEDVARSFHATAIALAHHADDQAETVLMRLLRGSGMTGLRGMSFRG
PGGRIRPLLNSRRCEIDAYLRARGLEHREDASNRDTAFLRNRIRHELLPLLEQYNPAIRERLVGTAALLADEDDLLEGMA
EDLAARACHFGSDSVSCSLTRLEGEPQALKRRLVRRILAHLTGNGEHVTRRHIQAMEDLMDSPRPNATLNLFRGIRAVRE
YGTLRLSRGLPPEDLPHEARELPGPGRYILSDGSLLTLAPATAPPDPASLPATVALFDLEKAPFPWRVRTFTPGDRIAPF
AMAGSKKVKELFMERRVPRDQRGRIPLIFCGDDLIWVCGLRTSRLAQLDAGSRNIIMAVYSPRSGDDRLTDAKERDREEK
PCAWE

Sequences:

>Translated_485_residues
MIATNLPARVSRTIRDQQLFAPGDTVILALSGGADSCVLLDILAGLTEFSPCLVIAHLNHQLRGAESDGDELFCRSLAER
YGLPFRSRSLDVAALARRQGLNLEDAGRRARLEFLEDVARSFHATAIALAHHADDQAETVLMRLLRGSGMTGLRGMSFRG
PGGRIRPLLNSRRCEIDAYLRARGLEHREDASNRDTAFLRNRIRHELLPLLEQYNPAIRERLVGTAALLADEDDLLEGMA
EDLAARACHFGSDSVSCSLTRLEGEPQALKRRLVRRILAHLTGNGEHVTRRHIQAMEDLMDSPRPNATLNLFRGIRAVRE
YGTLRLSRGLPPEDLPHEARELPGPGRYILSDGSLLTLAPATAPPDPASLPATVALFDLEKAPFPWRVRTFTPGDRIAPF
AMAGSKKVKELFMERRVPRDQRGRIPLIFCGDDLIWVCGLRTSRLAQLDAGSRNIIMAVYSPRSGDDRLTDAKERDREEK
PCAWE
>Mature_485_residues
MIATNLPARVSRTIRDQQLFAPGDTVILALSGGADSCVLLDILAGLTEFSPCLVIAHLNHQLRGAESDGDELFCRSLAER
YGLPFRSRSLDVAALARRQGLNLEDAGRRARLEFLEDVARSFHATAIALAHHADDQAETVLMRLLRGSGMTGLRGMSFRG
PGGRIRPLLNSRRCEIDAYLRARGLEHREDASNRDTAFLRNRIRHELLPLLEQYNPAIRERLVGTAALLADEDDLLEGMA
EDLAARACHFGSDSVSCSLTRLEGEPQALKRRLVRRILAHLTGNGEHVTRRHIQAMEDLMDSPRPNATLNLFRGIRAVRE
YGTLRLSRGLPPEDLPHEARELPGPGRYILSDGSLLTLAPATAPPDPASLPATVALFDLEKAPFPWRVRTFTPGDRIAPF
AMAGSKKVKELFMERRVPRDQRGRIPLIFCGDDLIWVCGLRTSRLAQLDAGSRNIIMAVYSPRSGDDRLTDAKERDREEK
PCAWE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=436, Percent_Identity=27.5229357798165, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24651972, Length=200, Percent_Identity=30.5, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 53884; Mature: 53884

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATNLPARVSRTIRDQQLFAPGDTVILALSGGADSCVLLDILAGLTEFSPCLVIAHLNH
CCCCCCCHHHHHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCH
QLRGAESDGDELFCRSLAERYGLPFRSRSLDVAALARRQGLNLEDAGRRARLEFLEDVAR
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHCCHHHHHHHHHHHHH
SFHATAIALAHHADDQAETVLMRLLRGSGMTGLRGMSFRGPGGRIRPLLNSRRCEIDAYL
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHH
RARGLEHREDASNRDTAFLRNRIRHELLPLLEQYNPAIRERLVGTAALLADEDDLLEGMA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHH
EDLAARACHFGSDSVSCSLTRLEGEPQALKRRLVRRILAHLTGNGEHVTRRHIQAMEDLM
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
DSPRPNATLNLFRGIRAVREYGTLRLSRGLPPEDLPHEARELPGPGRYILSDGSLLTLAP
HCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHCCCCCCEEEECCCEEEEEC
ATAPPDPASLPATVALFDLEKAPFPWRVRTFTPGDRIAPFAMAGSKKVKELFMERRVPRD
CCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCC
QRGRIPLIFCGDDLIWVCGLRTSRLAQLDAGSRNIIMAVYSPRSGDDRLTDAKERDREEK
CCCCEEEEEECCCEEEEECCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHCCCCC
PCAWE
CCCCC
>Mature Secondary Structure
MIATNLPARVSRTIRDQQLFAPGDTVILALSGGADSCVLLDILAGLTEFSPCLVIAHLNH
CCCCCCCHHHHHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCH
QLRGAESDGDELFCRSLAERYGLPFRSRSLDVAALARRQGLNLEDAGRRARLEFLEDVAR
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHCCHHHHHHHHHHHHH
SFHATAIALAHHADDQAETVLMRLLRGSGMTGLRGMSFRGPGGRIRPLLNSRRCEIDAYL
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHH
RARGLEHREDASNRDTAFLRNRIRHELLPLLEQYNPAIRERLVGTAALLADEDDLLEGMA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHH
EDLAARACHFGSDSVSCSLTRLEGEPQALKRRLVRRILAHLTGNGEHVTRRHIQAMEDLM
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
DSPRPNATLNLFRGIRAVREYGTLRLSRGLPPEDLPHEARELPGPGRYILSDGSLLTLAP
HCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHCCCCCCEEEECCCEEEEEC
ATAPPDPASLPATVALFDLEKAPFPWRVRTFTPGDRIAPFAMAGSKKVKELFMERRVPRD
CCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCC
QRGRIPLIFCGDDLIWVCGLRTSRLAQLDAGSRNIIMAVYSPRSGDDRLTDAKERDREEK
CCCCEEEEEECCCEEEEECCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHCCCCC
PCAWE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA