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The map label for this gene is lipA [H]

Identifier: 118580654

GI number: 118580654

Start: 2416983

End: 2417825

Strand: Direct

Name: lipA [H]

Synonym: Ppro_2239

Alternate gene names: 118580654

Gene position: 2416983-2417825 (Clockwise)

Preceding gene: 118580653

Following gene: 118580655

Centisome position: 60.3

GC content: 62.51

Gene sequence:

>843_bases
TTGAAGATTGAGCGCAAGCCGGAGTGGCTGCAAAAGAGGATCAATCCCGGAGGCCAGATCCCCATGCTGCGCCTTCTGGA
GGAGCTGAATCTGAACACGGTCTGCCGCCAGGCGCTCTGCCCCAACATCTCCGAGTGCTTCGCCCGCAACCAGGCGACCT
TCCTGATTCTGGGCAAAAACTGCACCCGCCACTGCTCCTTTTGCAATGTGGACAGCTGCGAACCAGAGGAGGTCGACCAA
GAGGAACCGCAACGCGTGGCCCAGGCGGTGGCCAGACTGGGGCTCTCCCACGTGGTCATCACCAGCCCGACCCGCGACGA
CCTTGATGACGGAGGTGCTAGCCTCTTTGCCCAGACCGTCAGGGCGATCCGCGCCGTCACGCCCTCTACGCGCATCGAAC
TGCTGATACCCGATTTCCTGGGAGACATCCCCAGCCTGGACAGGGTCATCGCCTCAGCACCGGACATCCTGGCCCACAAC
CTGGAAACAGTGCCACGGCTCTACCACATCCGCGCCGGCGCCGATTACAACCGCTCCCTGGAACTGCTGCGCCACTGCGG
CGAAACAGCTCCGGCTATCCGGCTCAAGTCCGGGATCATGCTCGGCCTGGGTGAGAAGGAACAGGAAATTCTGGAGCTGT
TTCGGGACCTGCGTGAGGTCGGCTGCGTGTACCTCAGCATCGGCCAGTACCTCGCCCCCAGCAAACGGCACCACCCGGTT
CGGGAGTACATCCATCCCGACCGGTTCGAATATCTGCGTGAACAGGCTCTCCAGATGGGCTTTGTCCACCTGGAGAGCGG
CCCCTACGTGCGCAGCTCCTACCACGCCGGTAGCTACAGCTGA

Upstream 100 bases:

>100_bases
GCCGTGAAGAACCTCTCTCACAACAGGAGCAGTCATGGGCCAAGGCGCTGATTGCAAACAAATATTCAACTGACACCTGG
AATCTGGAGGGGGTGGAAAA

Downstream 100 bases:

>100_bases
TAAGACACAAAAAAAGCCGGCCAAAATGGCCGGCTTTTTTTGTGAAAGGAAAGAGATCTGTTCAGGCGTAGATCGAGAAA
GCGTACGAACTGTCAGGAAC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKIERKPEWLQKRINPGGQIPMLRLLEELNLNTVCRQALCPNISECFARNQATFLILGKNCTRHCSFCNVDSCEPEEVDQ
EEPQRVAQAVARLGLSHVVITSPTRDDLDDGGASLFAQTVRAIRAVTPSTRIELLIPDFLGDIPSLDRVIASAPDILAHN
LETVPRLYHIRAGADYNRSLELLRHCGETAPAIRLKSGIMLGLGEKEQEILELFRDLREVGCVYLSIGQYLAPSKRHHPV
REYIHPDRFEYLREQALQMGFVHLESGPYVRSSYHAGSYS

Sequences:

>Translated_280_residues
MKIERKPEWLQKRINPGGQIPMLRLLEELNLNTVCRQALCPNISECFARNQATFLILGKNCTRHCSFCNVDSCEPEEVDQ
EEPQRVAQAVARLGLSHVVITSPTRDDLDDGGASLFAQTVRAIRAVTPSTRIELLIPDFLGDIPSLDRVIASAPDILAHN
LETVPRLYHIRAGADYNRSLELLRHCGETAPAIRLKSGIMLGLGEKEQEILELFRDLREVGCVYLSIGQYLAPSKRHHPV
REYIHPDRFEYLREQALQMGFVHLESGPYVRSSYHAGSYS
>Mature_280_residues
MKIERKPEWLQKRINPGGQIPMLRLLEELNLNTVCRQALCPNISECFARNQATFLILGKNCTRHCSFCNVDSCEPEEVDQ
EEPQRVAQAVARLGLSHVVITSPTRDDLDDGGASLFAQTVRAIRAVTPSTRIELLIPDFLGDIPSLDRVIASAPDILAHN
LETVPRLYHIRAGADYNRSLELLRHCGETAPAIRLKSGIMLGLGEKEQEILELFRDLREVGCVYLSIGQYLAPSKRHHPV
REYIHPDRFEYLREQALQMGFVHLESGPYVRSSYHAGSYS

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=284, Percent_Identity=41.5492957746479, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=40.8163265306122, Blast_Score=188, Evalue=4e-48,
Organism=Escherichia coli, GI1786846, Length=276, Percent_Identity=48.9130434782609, Blast_Score=269, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=40.418118466899, Blast_Score=214, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6324770, Length=280, Percent_Identity=44.2857142857143, Blast_Score=241, Evalue=6e-65,
Organism=Drosophila melanogaster, GI221513272, Length=284, Percent_Identity=40.1408450704225, Blast_Score=221, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 31599; Mature: 31599

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIERKPEWLQKRINPGGQIPMLRLLEELNLNTVCRQALCPNISECFARNQATFLILGKN
CCCCCCHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHCCCCEEEEECCC
CTRHCSFCNVDSCEPEEVDQEEPQRVAQAVARLGLSHVVITSPTRDDLDDGGASLFAQTV
CCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHH
RAIRAVTPSTRIELLIPDFLGDIPSLDRVIASAPDILAHNLETVPRLYHIRAGADYNRSL
HHHHHCCCCCCEEEEECHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCHHHH
ELLRHCGETAPAIRLKSGIMLGLGEKEQEILELFRDLREVGCVYLSIGQYLAPSKRHHPV
HHHHHCCCCCCCEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
REYIHPDRFEYLREQALQMGFVHLESGPYVRSSYHAGSYS
HHHCCCHHHHHHHHHHHHHHHEEECCCCCEECCCCCCCCC
>Mature Secondary Structure
MKIERKPEWLQKRINPGGQIPMLRLLEELNLNTVCRQALCPNISECFARNQATFLILGKN
CCCCCCHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHCCCCEEEEECCC
CTRHCSFCNVDSCEPEEVDQEEPQRVAQAVARLGLSHVVITSPTRDDLDDGGASLFAQTV
CCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHH
RAIRAVTPSTRIELLIPDFLGDIPSLDRVIASAPDILAHNLETVPRLYHIRAGADYNRSL
HHHHHCCCCCCEEEEECHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCHHHH
ELLRHCGETAPAIRLKSGIMLGLGEKEQEILELFRDLREVGCVYLSIGQYLAPSKRHHPV
HHHHHCCCCCCCEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
REYIHPDRFEYLREQALQMGFVHLESGPYVRSSYHAGSYS
HHHCCCHHHHHHHHHHHHHHHEEECCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA