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The map label for this gene is cca [H]

Identifier: 118580470

GI number: 118580470

Start: 2202465

End: 2203679

Strand: Direct

Name: cca [H]

Synonym: Ppro_2054

Alternate gene names: 118580470

Gene position: 2202465-2203679 (Clockwise)

Preceding gene: 118580468

Following gene: 118580471

Centisome position: 54.95

GC content: 63.54

Gene sequence:

>1215_bases
ATGAACGAAGTGCGATCGCAACTGGCTGAGTTGTTCCCACCGGCATGCCATGGCCGGGTCTTTCTGGTGGGAGGATGTGT
ACGGGACCACCTGCTGGGCCGCGGGTGCCGTGACATCGACCTGGTGGCGGCGCTCAGCGGGGATGAGCTCGGCAGGTGCG
GGTTCCGGCTGGTGGCCGGCAGGAGCACCGCGCCGATCTGGTTCAGGCACGATGATGCATTCGGCACCATCGAACTGACG
CCGCTGACCTGCCCGGAGGCTTTGGACGCCGACCTGCGGCGCCGCGACTTCACCATCAATAGCCTGGCCATGGATCTTCG
GGGGAACCTGCACGACCCGTTTGACGGTCGCGGCGACATCATGGGGCGTTTTTTGCGCCCCTGCTCCCCAGACACCTTCA
GGGACGACCCCCTGCGCATCTTCCGGGCATTCCGCTTCGAATCCGACGGATGGAGCATGACCGGCGAGTGCCAGTATTTG
ATCGGGCAGCACCAGTGGTCGGAAGAGCTGTCCCGCATCCCCATGGAGCGTTTCAGCCGCGAGATGCTCAAGGCCTGCTC
TGCCGCCCGACCGGAACGTTTCTTCCTGGCCATGCTGAAATTCGGCGTGGGCGCCGGATATCTGCCGGAACTCTTCCGCA
TGGCCCGTATTCCTGCCGGCCCCCTCCGTTACCATCCCGAGGGGGATCTTTTGACCCACTCCATCCAGGTGATGCAACGG
GTGGCAGAGCGCTCCACCGATCCCTTGGCCCGCTTCTGCGCCCTGTTCCACGACATCGGCAAGCTGTCCACCGACCCTGC
CCGCTACCCCAGCCACCATGGCCATGACCGGTCCGGTGTCGAACTGGCCCGCCAGCTCTGCTCCCGCCTGCGCCTGTCGG
CCAGGTACCGTAACGCCTTGGCCGCTATCTGCCGGCTGCATATGACATTCAACAACTGGGAAGAGTTACGGGACGGGACA
CGGCTGAGAGCTGCCGAGCAGGCGGTCAAATCGGGCATTACCGGAATCCTGCCCCTGGTTTCCGCGGCGGACAGGGCAAG
ATCCGAAGAACCGGCCGGATGGTCGCGGGCGGTACAGGTTGTCCGCATGTCAACAATCGAACTGGGCATGGATCAGCAAC
AGCTGGAAAAGATGCCAGCCGACCTTCGCAGCGATCTCATCCTGCAGCTTCGCATAGAGAGATTTCGTCAGGCAACGGGC
TGTTCGGCGAACTGA

Upstream 100 bases:

>100_bases
CGTTAAACTGTCCTGCTTTCTACATGAGCGCACCCTCCCTGTCAACCATGGCGCGGGGTGAGGGACTGGCACTGATACCC
ACACGGGGGGAGCATCTGCC

Downstream 100 bases:

>100_bases
GACGCCAGCGGAGAGAACCCCGAGCGCATTCCCCGTTGAGCGTCGGTTACGGGGAGTTTCTGCGCCAGCAGAGAACGACT
CCGCCGACACAACACCCCTG

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MNEVRSQLAELFPPACHGRVFLVGGCVRDHLLGRGCRDIDLVAALSGDELGRCGFRLVAGRSTAPIWFRHDDAFGTIELT
PLTCPEALDADLRRRDFTINSLAMDLRGNLHDPFDGRGDIMGRFLRPCSPDTFRDDPLRIFRAFRFESDGWSMTGECQYL
IGQHQWSEELSRIPMERFSREMLKACSAARPERFFLAMLKFGVGAGYLPELFRMARIPAGPLRYHPEGDLLTHSIQVMQR
VAERSTDPLARFCALFHDIGKLSTDPARYPSHHGHDRSGVELARQLCSRLRLSARYRNALAAICRLHMTFNNWEELRDGT
RLRAAEQAVKSGITGILPLVSAADRARSEEPAGWSRAVQVVRMSTIELGMDQQQLEKMPADLRSDLILQLRIERFRQATG
CSAN

Sequences:

>Translated_404_residues
MNEVRSQLAELFPPACHGRVFLVGGCVRDHLLGRGCRDIDLVAALSGDELGRCGFRLVAGRSTAPIWFRHDDAFGTIELT
PLTCPEALDADLRRRDFTINSLAMDLRGNLHDPFDGRGDIMGRFLRPCSPDTFRDDPLRIFRAFRFESDGWSMTGECQYL
IGQHQWSEELSRIPMERFSREMLKACSAARPERFFLAMLKFGVGAGYLPELFRMARIPAGPLRYHPEGDLLTHSIQVMQR
VAERSTDPLARFCALFHDIGKLSTDPARYPSHHGHDRSGVELARQLCSRLRLSARYRNALAAICRLHMTFNNWEELRDGT
RLRAAEQAVKSGITGILPLVSAADRARSEEPAGWSRAVQVVRMSTIELGMDQQQLEKMPADLRSDLILQLRIERFRQATG
CSAN
>Mature_404_residues
MNEVRSQLAELFPPACHGRVFLVGGCVRDHLLGRGCRDIDLVAALSGDELGRCGFRLVAGRSTAPIWFRHDDAFGTIELT
PLTCPEALDADLRRRDFTINSLAMDLRGNLHDPFDGRGDIMGRFLRPCSPDTFRDDPLRIFRAFRFESDGWSMTGECQYL
IGQHQWSEELSRIPMERFSREMLKACSAARPERFFLAMLKFGVGAGYLPELFRMARIPAGPLRYHPEGDLLTHSIQVMQR
VAERSTDPLARFCALFHDIGKLSTDPARYPSHHGHDRSGVELARQLCSRLRLSARYRNALAAICRLHMTFNNWEELRDGT
RLRAAEQAVKSGITGILPLVSAADRARSEEPAGWSRAVQVVRMSTIELGMDQQQLEKMPADLRSDLILQLRIERFRQATG
CSAN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=324, Percent_Identity=33.641975308642, Blast_Score=154, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45626; Mature: 45626

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVRSQLAELFPPACHGRVFLVGGCVRDHLLGRGCRDIDLVAALSGDELGRCGFRLVAG
CCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCHHHEEECCCCCCCCCCEEEEEC
RSTAPIWFRHDDAFGTIELTPLTCPEALDADLRRRDFTINSLAMDLRGNLHDPFDGRGDI
CCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHH
MGRFLRPCSPDTFRDDPLRIFRAFRFESDGWSMTGECQYLIGQHQWSEELSRIPMERFSR
HHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHCCHHHHHH
EMLKACSAARPERFFLAMLKFGVGAGYLPELFRMARIPAGPLRYHPEGDLLTHSIQVMQR
HHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VAERSTDPLARFCALFHDIGKLSTDPARYPSHHGHDRSGVELARQLCSRLRLSARYRNAL
HHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AAICRLHMTFNNWEELRDGTRLRAAEQAVKSGITGILPLVSAADRARSEEPAGWSRAVQV
HHHHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
VRMSTIELGMDQQQLEKMPADLRSDLILQLRIERFRQATGCSAN
HHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNEVRSQLAELFPPACHGRVFLVGGCVRDHLLGRGCRDIDLVAALSGDELGRCGFRLVAG
CCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCHHHEEECCCCCCCCCCEEEEEC
RSTAPIWFRHDDAFGTIELTPLTCPEALDADLRRRDFTINSLAMDLRGNLHDPFDGRGDI
CCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHH
MGRFLRPCSPDTFRDDPLRIFRAFRFESDGWSMTGECQYLIGQHQWSEELSRIPMERFSR
HHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHCCHHHHHH
EMLKACSAARPERFFLAMLKFGVGAGYLPELFRMARIPAGPLRYHPEGDLLTHSIQVMQR
HHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VAERSTDPLARFCALFHDIGKLSTDPARYPSHHGHDRSGVELARQLCSRLRLSARYRNAL
HHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AAICRLHMTFNNWEELRDGTRLRAAEQAVKSGITGILPLVSAADRARSEEPAGWSRAVQV
HHHHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
VRMSTIELGMDQQQLEKMPADLRSDLILQLRIERFRQATGCSAN
HHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA