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The map label for this gene is pilT [H]

Identifier: 118580447

GI number: 118580447

Start: 2180689

End: 2181759

Strand: Direct

Name: pilT [H]

Synonym: Ppro_2031

Alternate gene names: 118580447

Gene position: 2180689-2181759 (Clockwise)

Preceding gene: 118580446

Following gene: 118580449

Centisome position: 54.41

GC content: 58.08

Gene sequence:

>1071_bases
ATGGACATCAACATACTGCACCAGATCCTGGAAATCGCCTTTCAGAAACGGGTTTCCGACCTCCATTTCGAGGTCGACAA
TCCCCCCTTTTTCCGGGGACGCGGGCAACTGATCCGCTCCAAGATGCAGAATCTGACCCCCGGGGACACGGAGTTCATAG
CAGCACGCGTCCTGGAGCAGAACAATCGCCAGATCAATAGTGATTTCAAGGAACTTGACGCGTCATACGCTTTACCCAGC
GGAGGGCGCTTCCGTGCCAGCGTCTTCAAGCAGCGCGGACACTTCGGCATCGTCATGCGTGTCATCCCTCCCCACGTCAG
TACCTTCCAGGAGCTGAACCTGCCGCCGGTGCTGGAGGAGATTGCCAAGGCGCCCAACGGCCTGATCCTGGTCACCGGCC
CCACCGGTAACGGCAAATCCACCACCCTGGCAGCCATGATCAGATGCATCAACGAACACAACAACTACAACATCATAACC
ATAGAAGATCCCATTGAATTCCTCTTCACCTCCCAGAGGAGTTGCATCATCCAGAGGGAGGTCGGCATCGACACCGCCAG
CTTCAACAGCGCCCTGAAGGCGGCTCTGCGCATGGATCCGGATGTAATCATGGTGGGAGAAATGCGCGACACGGAAACCA
TCGACGCCTGCATCAAGGCGGCGGAGACCGGCCATCTGGTCCTGACCACCCTGCACACCCAGGGTGCCGTCTCCACCATA
AACCGCATCGTGGGCAACTTCCCGCCCGACACGCAGGAGATCATCCGCCAGCGGTTGGCCGATATCCTCGTGGCCACCAT
CTCGCTGCGACTGGTAAAGGACAAAACCGGCGAGAGCATCCTGCCGGTGGTGGAGATCATGCGCGCCACCACGACCATCC
AGGCCTGCATCCGCGAGGGGCGGCTGAGCGAACTGGAAAAGCATATCGAAAACGGCAGTTCCCAGTACCAGATGCAGTCT
TTGGACCAACACCTGCTGCAGCTCTACAAACAGGGGCAGATCACCATCGAGGATGCCAAGCGTCTGACCCACTCCATGGA
CCTGGAACGGAAGCTTATGTTCGATTCATAG

Upstream 100 bases:

>100_bases
TGCCGGACTGCTGCTGGACAACAGCCTGTACCGGAAGAGATATCTCGCCTCGGCCCAGAAGAGCAACCAGAACACCCCGC
AGTCATAACAAGGAGAATCC

Downstream 100 bases:

>100_bases
GGAAGCAGGGCCTAAACGGGGAAGGAGTTTTATCTGCTAGCCGCGTGCTGCGGGCAGGTACTCGCCGTTGATCACATTGA
CCGGCGCAGCGGCCAGAAAC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MDINILHQILEIAFQKRVSDLHFEVDNPPFFRGRGQLIRSKMQNLTPGDTEFIAARVLEQNNRQINSDFKELDASYALPS
GGRFRASVFKQRGHFGIVMRVIPPHVSTFQELNLPPVLEEIAKAPNGLILVTGPTGNGKSTTLAAMIRCINEHNNYNIIT
IEDPIEFLFTSQRSCIIQREVGIDTASFNSALKAALRMDPDVIMVGEMRDTETIDACIKAAETGHLVLTTLHTQGAVSTI
NRIVGNFPPDTQEIIRQRLADILVATISLRLVKDKTGESILPVVEIMRATTTIQACIREGRLSELEKHIENGSSQYQMQS
LDQHLLQLYKQGQITIEDAKRLTHSMDLERKLMFDS

Sequences:

>Translated_356_residues
MDINILHQILEIAFQKRVSDLHFEVDNPPFFRGRGQLIRSKMQNLTPGDTEFIAARVLEQNNRQINSDFKELDASYALPS
GGRFRASVFKQRGHFGIVMRVIPPHVSTFQELNLPPVLEEIAKAPNGLILVTGPTGNGKSTTLAAMIRCINEHNNYNIIT
IEDPIEFLFTSQRSCIIQREVGIDTASFNSALKAALRMDPDVIMVGEMRDTETIDACIKAAETGHLVLTTLHTQGAVSTI
NRIVGNFPPDTQEIIRQRLADILVATISLRLVKDKTGESILPVVEIMRATTTIQACIREGRLSELEKHIENGSSQYQMQS
LDQHLLQLYKQGQITIEDAKRLTHSMDLERKLMFDS
>Mature_356_residues
MDINILHQILEIAFQKRVSDLHFEVDNPPFFRGRGQLIRSKMQNLTPGDTEFIAARVLEQNNRQINSDFKELDASYALPS
GGRFRASVFKQRGHFGIVMRVIPPHVSTFQELNLPPVLEEIAKAPNGLILVTGPTGNGKSTTLAAMIRCINEHNNYNIIT
IEDPIEFLFTSQRSCIIQREVGIDTASFNSALKAALRMDPDVIMVGEMRDTETIDACIKAAETGHLVLTTLHTQGAVSTI
NRIVGNFPPDTQEIIRQRLADILVATISLRLVKDKTGESILPVVEIMRATTTIQACIREGRLSELEKHIENGSSQYQMQS
LDQHLLQLYKQGQITIEDAKRLTHSMDLERKLMFDS

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=307, Percent_Identity=41.6938110749186, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1786296, Length=280, Percent_Identity=33.5714285714286, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789723, Length=126, Percent_Identity=47.6190476190476, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39959; Mature: 39959

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDINILHQILEIAFQKRVSDLHFEVDNPPFFRGRGQLIRSKMQNLTPGDTEFIAARVLEQ
CCHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
NNRQINSDFKELDASYALPSGGRFRASVFKQRGHFGIVMRVIPPHVSTFQELNLPPVLEE
CCCHHHCHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCHHHHH
IAKAPNGLILVTGPTGNGKSTTLAAMIRCINEHNNYNIITIEDPIEFLFTSQRSCIIQRE
HHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHCCCCCEEEEHH
VGIDTASFNSALKAALRMDPDVIMVGEMRDTETIDACIKAAETGHLVLTTLHTQGAVSTI
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHH
NRIVGNFPPDTQEIIRQRLADILVATISLRLVKDKTGESILPVVEIMRATTTIQACIREG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
RLSELEKHIENGSSQYQMQSLDQHLLQLYKQGQITIEDAKRLTHSMDLERKLMFDS
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCHHHHHCCCC
>Mature Secondary Structure
MDINILHQILEIAFQKRVSDLHFEVDNPPFFRGRGQLIRSKMQNLTPGDTEFIAARVLEQ
CCHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
NNRQINSDFKELDASYALPSGGRFRASVFKQRGHFGIVMRVIPPHVSTFQELNLPPVLEE
CCCHHHCHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCHHHHH
IAKAPNGLILVTGPTGNGKSTTLAAMIRCINEHNNYNIITIEDPIEFLFTSQRSCIIQRE
HHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHCCCCCEEEEHH
VGIDTASFNSALKAALRMDPDVIMVGEMRDTETIDACIKAAETGHLVLTTLHTQGAVSTI
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHH
NRIVGNFPPDTQEIIRQRLADILVATISLRLVKDKTGESILPVVEIMRATTTIQACIREG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
RLSELEKHIENGSSQYQMQSLDQHLLQLYKQGQITIEDAKRLTHSMDLERKLMFDS
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]