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The map label for this gene is mdeA [H]

Identifier: 118580207

GI number: 118580207

Start: 1914055

End: 1915341

Strand: Reverse

Name: mdeA [H]

Synonym: Ppro_1787

Alternate gene names: 118580207

Gene position: 1915341-1914055 (Counterclockwise)

Preceding gene: 118580208

Following gene: 118580206

Centisome position: 47.79

GC content: 61.93

Gene sequence:

>1287_bases
ATGGAATCTCAGCAAAAATCCATAGAAACGCTTGCGCTTCATGCAGGGCAGTCTCCGGACTCGGAAACCCTATCCCGGGC
AGTGCCGATTTACCAAACCTCGTCCTATGTGTTCAGGAACTCTGAGCATGCCGCCAACCTCTTCGGACTAAAGGAACCGG
GCAACATCTACACCCGGCTGATGAACCCTACCACCGACGTGCTGGAACGGCGCATGGCGGCCCTTGACGGCGGCGTCGGC
GCACTGGCGGTAGCCTCCGGCCAGGCAGCCATCACCTACGCGGTGCTGAACATCACCAGTGCCGGGCAGAATATCGTCTC
CACCAGCTTTCTCTACGGCGGCACCTACAACCTGTTCCACTACACCCTCCCCAGGCTCGGCATCCAGGTAAAATTCGTCG
ACTCCTCGGATCCGGAGAACATCCGCCGGGCCATCGACCAGGACACGCGCCTGGTGTACAGCGAATCGGTGGGCAACCCC
AAGAACAACGTGGATGATTTCGAGGCCATCGCAGCCATCGCCCACGAGGCCGGCATACCGCTGGTCGTGGACAACACGGT
GACGACTCCCTGGCTCTTCAAGCCGCTGGACCATGGGGCGGACATCGTGGTCTATTCGCTGACAAAATTCATCGCCGGCC
ACGGCACCAGCATCGGCGGCGCCATCGTGGACGGCGGAACGTTCAACTGGGATAACGGGAGATTCCCCGAACTCACCGAG
CCCGATCCCTCCTACCATGGCCTGAAGTACTGGGAGGCGCTGGGCAGCCAGGCCTACATCCTCAAGATGAGGGTCACGCT
GCTGCGCGACATGGGGGCCTGTCTCTCCCCCTTCAACTCCTTCCAGATCCTGCAGGGGCTGGAGACGCTGCACGTGCGCA
TGCCACGCCATGTGGAAAACGCCCGTTGCGTCGCATCCTGGCTGGAGCGCCACCCCCTGGTGAGCTGGGTCAACTACCCG
GGCCTCGCCAGCCATCGGGATCACGCCAATGCACGCAAATACCTGCCCAAGGGGGAGGGGGCCATCATCGGCTTCGGAAT
CAAGGGGGGAGTGAAGGCAGGCAGCAAATTCATCGACAACGTGAAGCTGCTCTCCCACCTGGCCAACATCGGCGATGCCA
AGTCGCTGGTCATCCACCCCGCCTCCACCACCCACCAGCAGCTGTCCGAGGAGGAACAGCTGGCCAGCGGCGTGTCGCCG
GACTTCATCCGCCTTTCCATCGGCCTGGAGAACATCGACGACATCATCGCCGATATCAGCCAGGCGCTGGAGGCGTCACA
GCTCTAG

Upstream 100 bases:

>100_bases
CCCTTATCCGGTGTCAGGTGTCAGTTGCCGGTCCGCCAGAAGCAGCCAACAATCAGCAACGTCATCCATCGCATCAAGAT
GCACACTTCTCGGAGGAACC

Downstream 100 bases:

>100_bases
CCGCCACGTCATTCACGACAGCTGCTGTTGTGTGTAGTAGTGCCTCATTGCATGCCATCACCCTACCTATTGGAATCAGG
AGCATTTCATGTACAAGTTG

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MESQQKSIETLALHAGQSPDSETLSRAVPIYQTSSYVFRNSEHAANLFGLKEPGNIYTRLMNPTTDVLERRMAALDGGVG
ALAVASGQAAITYAVLNITSAGQNIVSTSFLYGGTYNLFHYTLPRLGIQVKFVDSSDPENIRRAIDQDTRLVYSESVGNP
KNNVDDFEAIAAIAHEAGIPLVVDNTVTTPWLFKPLDHGADIVVYSLTKFIAGHGTSIGGAIVDGGTFNWDNGRFPELTE
PDPSYHGLKYWEALGSQAYILKMRVTLLRDMGACLSPFNSFQILQGLETLHVRMPRHVENARCVASWLERHPLVSWVNYP
GLASHRDHANARKYLPKGEGAIIGFGIKGGVKAGSKFIDNVKLLSHLANIGDAKSLVIHPASTTHQQLSEEEQLASGVSP
DFIRLSIGLENIDDIIADISQALEASQL

Sequences:

>Translated_428_residues
MESQQKSIETLALHAGQSPDSETLSRAVPIYQTSSYVFRNSEHAANLFGLKEPGNIYTRLMNPTTDVLERRMAALDGGVG
ALAVASGQAAITYAVLNITSAGQNIVSTSFLYGGTYNLFHYTLPRLGIQVKFVDSSDPENIRRAIDQDTRLVYSESVGNP
KNNVDDFEAIAAIAHEAGIPLVVDNTVTTPWLFKPLDHGADIVVYSLTKFIAGHGTSIGGAIVDGGTFNWDNGRFPELTE
PDPSYHGLKYWEALGSQAYILKMRVTLLRDMGACLSPFNSFQILQGLETLHVRMPRHVENARCVASWLERHPLVSWVNYP
GLASHRDHANARKYLPKGEGAIIGFGIKGGVKAGSKFIDNVKLLSHLANIGDAKSLVIHPASTTHQQLSEEEQLASGVSP
DFIRLSIGLENIDDIIADISQALEASQL
>Mature_428_residues
MESQQKSIETLALHAGQSPDSETLSRAVPIYQTSSYVFRNSEHAANLFGLKEPGNIYTRLMNPTTDVLERRMAALDGGVG
ALAVASGQAAITYAVLNITSAGQNIVSTSFLYGGTYNLFHYTLPRLGIQVKFVDSSDPENIRRAIDQDTRLVYSESVGNP
KNNVDDFEAIAAIAHEAGIPLVVDNTVTTPWLFKPLDHGADIVVYSLTKFIAGHGTSIGGAIVDGGTFNWDNGRFPELTE
PDPSYHGLKYWEALGSQAYILKMRVTLLRDMGACLSPFNSFQILQGLETLHVRMPRHVENARCVASWLERHPLVSWVNYP
GLASHRDHANARKYLPKGEGAIIGFGIKGGVKAGSKFIDNVKLLSHLANIGDAKSLVIHPASTTHQQLSEEEQLASGVSP
DFIRLSIGLENIDDIIADISQALEASQL

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=420, Percent_Identity=34.7619047619048, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI299473759, Length=420, Percent_Identity=31.6666666666667, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI25453487, Length=419, Percent_Identity=30.0715990453461, Blast_Score=152, Evalue=8e-37,
Organism=Escherichia coli, GI1790375, Length=418, Percent_Identity=30.622009569378, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1789383, Length=421, Percent_Identity=27.0783847980998, Blast_Score=135, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71980708, Length=407, Percent_Identity=31.2039312039312, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17538055, Length=420, Percent_Identity=34.0476190476191, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17560056, Length=420, Percent_Identity=34.5238095238095, Blast_Score=198, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=53.7037037037037, Blast_Score=423, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319307, Length=422, Percent_Identity=33.4123222748815, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321170, Length=353, Percent_Identity=26.3456090651558, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321254, Length=444, Percent_Identity=26.8018018018018, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=428, Percent_Identity=24.7663551401869, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922554, Length=425, Percent_Identity=35.2941176470588, Blast_Score=218, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46565; Mature: 46565

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESQQKSIETLALHAGQSPDSETLSRAVPIYQTSSYVFRNSEHAANLFGLKEPGNIYTRL
CCCHHHHHHHHHHCCCCCCCHHHHHHCCCCEECCCCEEECCCCHHHCCCCCCCCHHHEEE
MNPTTDVLERRMAALDGGVGALAVASGQAAITYAVLNITSAGQNIVSTSFLYGGTYNLFH
CCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEEECCCCCCHHHHHEEECCCHHHHH
YTLPRLGIQVKFVDSSDPENIRRAIDQDTRLVYSESVGNPKNNVDDFEAIAAIAHEAGIP
HHHHHCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC
LVVDNTVTTPWLFKPLDHGADIVVYSLTKFIAGHGTSIGGAIVDGGTFNWDNGRFPELTE
EEECCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCEECCCCCCCCCCCC
PDPSYHGLKYWEALGSQAYILKMRVTLLRDMGACLSPFNSFQILQGLETLHVRMPRHVEN
CCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHH
ARCVASWLERHPLVSWVNYPGLASHRDHANARKYLPKGEGAIIGFGIKGGVKAGSKFIDN
HHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHCCCCCCCCEEEEECCCCHHHHHHHHHH
VKLLSHLANIGDAKSLVIHPASTTHQQLSEEEQLASGVSPDFIRLSIGLENIDDIIADIS
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
QALEASQL
HHHHHCCC
>Mature Secondary Structure
MESQQKSIETLALHAGQSPDSETLSRAVPIYQTSSYVFRNSEHAANLFGLKEPGNIYTRL
CCCHHHHHHHHHHCCCCCCCHHHHHHCCCCEECCCCEEECCCCHHHCCCCCCCCHHHEEE
MNPTTDVLERRMAALDGGVGALAVASGQAAITYAVLNITSAGQNIVSTSFLYGGTYNLFH
CCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEEECCCCCCHHHHHEEECCCHHHHH
YTLPRLGIQVKFVDSSDPENIRRAIDQDTRLVYSESVGNPKNNVDDFEAIAAIAHEAGIP
HHHHHCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC
LVVDNTVTTPWLFKPLDHGADIVVYSLTKFIAGHGTSIGGAIVDGGTFNWDNGRFPELTE
EEECCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCEECCCCCCCCCCCC
PDPSYHGLKYWEALGSQAYILKMRVTLLRDMGACLSPFNSFQILQGLETLHVRMPRHVEN
CCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHH
ARCVASWLERHPLVSWVNYPGLASHRDHANARKYLPKGEGAIIGFGIKGGVKAGSKFIDN
HHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHCCCCCCCCEEEEECCCCHHHHHHHHHH
VKLLSHLANIGDAKSLVIHPASTTHQQLSEEEQLASGVSPDFIRLSIGLENIDDIIADIS
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
QALEASQL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]