Click here to switch to the map view.

The map label for this gene is yjbJ [H]

Identifier: 118580189

GI number: 118580189

Start: 1899790

End: 1900359

Strand: Reverse

Name: yjbJ [H]

Synonym: Ppro_1769

Alternate gene names: 118580189

Gene position: 1900359-1899790 (Counterclockwise)

Preceding gene: 118580191

Following gene: 118580188

Centisome position: 47.41

GC content: 52.98

Gene sequence:

>570_bases
ATGGTGGCCCGCCAATTTTTTACTAAAAGTCAAGCAAAAAAAATGTGGCATCACGCTGATTTTGTTACTTTTTTACATTC
TCAAAGAACCATGCAAAGACCTAACCGGACGACGCTGATGCGGGATATAAAAAAGGACAAGCTGCTGCGCACCAAATTCA
GACTGATCAACGCGGCCAATCCGTCCGAATACGAACCGCTGATCAGAACCTGCTCGGAAAAATACGGTGTCAATTCATCA
CTGATCAAGGCGGTCATTCACGCCGAATCCGGTTACAATCCCAACGCGGTTTCCAGCAAGGGCGCCAGCGGCCTCATGCA
ACTCATGCCCGGCACCGCCCGGTCGCTGAACGTGGCCAACAGCCTCGATCCCAAAGACAACGTGGAGGGCGGAGTCAAGT
ATCTCCGCTTTCTGCTGGACACCTTCCACGGCGACGTCTCCCTGGCCCTAGCGGCGTACAATGCCGGACTAGGCAAAGTG
GCCAGATACGGCGGCATTCCTCCCTATACCGAGACCCGTACCTATGTAAACCGGGTACTTTCCTACATGCAATCCTACCA
GGCCAACTGA

Upstream 100 bases:

>100_bases
AACCGGGGTAAGAAGCAATTTGAAAGCACGTGCTTTTCGTCGTTGTTTCAGTCGCTTGAAAGGTCGCATGTCGTAACTCG
TGTAATATTAAGTAGTTACG

Downstream 100 bases:

>100_bases
TCGATGACGACACAGACGCAAACCCCGATTCTCAACCCGCCCAACATCCTGACCATGCTGCGCATCGCGGCCATACCGCT
CCTGGCGACGCTGCTCATGT

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MVARQFFTKSQAKKMWHHADFVTFLHSQRTMQRPNRTTLMRDIKKDKLLRTKFRLINAANPSEYEPLIRTCSEKYGVNSS
LIKAVIHAESGYNPNAVSSKGASGLMQLMPGTARSLNVANSLDPKDNVEGGVKYLRFLLDTFHGDVSLALAAYNAGLGKV
ARYGGIPPYTETRTYVNRVLSYMQSYQAN

Sequences:

>Translated_189_residues
MVARQFFTKSQAKKMWHHADFVTFLHSQRTMQRPNRTTLMRDIKKDKLLRTKFRLINAANPSEYEPLIRTCSEKYGVNSS
LIKAVIHAESGYNPNAVSSKGASGLMQLMPGTARSLNVANSLDPKDNVEGGVKYLRFLLDTFHGDVSLALAAYNAGLGKV
ARYGGIPPYTETRTYVNRVLSYMQSYQAN
>Mature_189_residues
MVARQFFTKSQAKKMWHHADFVTFLHSQRTMQRPNRTTLMRDIKKDKLLRTKFRLINAANPSEYEPLIRTCSEKYGVNSS
LIKAVIHAESGYNPNAVSSKGASGLMQLMPGTARSLNVANSLDPKDNVEGGVKYLRFLLDTFHGDVSLALAAYNAGLGKV
ARYGGIPPYTETRTYVNRVLSYMQSYQAN

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=145, Percent_Identity=33.1034482758621, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI87082191, Length=118, Percent_Identity=35.5932203389831, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 21212; Mature: 21212

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVARQFFTKSQAKKMWHHADFVTFLHSQRTMQRPNRTTLMRDIKKDKLLRTKFRLINAAN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PSEYEPLIRTCSEKYGVNSSLIKAVIHAESGYNPNAVSSKGASGLMQLMPGTARSLNVAN
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHCCCCC
SLDPKDNVEGGVKYLRFLLDTFHGDVSLALAAYNAGLGKVARYGGIPPYTETRTYVNRVL
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHH
SYMQSYQAN
HHHHHHCCC
>Mature Secondary Structure
MVARQFFTKSQAKKMWHHADFVTFLHSQRTMQRPNRTTLMRDIKKDKLLRTKFRLINAAN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PSEYEPLIRTCSEKYGVNSSLIKAVIHAESGYNPNAVSSKGASGLMQLMPGTARSLNVAN
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHCCCCC
SLDPKDNVEGGVKYLRFLLDTFHGDVSLALAAYNAGLGKVARYGGIPPYTETRTYVNRVL
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHH
SYMQSYQAN
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]