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The map label for this gene is yjeA [H]

Identifier: 118580184

GI number: 118580184

Start: 1894689

End: 1895594

Strand: Direct

Name: yjeA [H]

Synonym: Ppro_1764

Alternate gene names: 118580184

Gene position: 1894689-1895594 (Clockwise)

Preceding gene: 118580183

Following gene: 118580186

Centisome position: 47.27

GC content: 58.72

Gene sequence:

>906_bases
ATGCACCCCCGCGACCACAACCTCTATCTGAGGGCGGACATCGTAGCCGCCATCAGGTCTTTCTTTGCTTCACGTCGTTT
TCTGGAGGTGGATACGCCCCATCTTATTCCCGCCAACGCGCCTGAAGAGTATATCGATCCCCATGGTTCTGGCGACCGGT
ACCTGCATACCTCGCCGGAGATCTGCATGAAGCGCCTGCTCTGTCGCGGCCATGAGCGGATTTTTCAGATCTGCCGTTGC
TGGCGCAGGGATGAACGGGGAAGTCGCCATCTGCCGGAGTTTGCCATGCTGGAATGGTATCGGCGCGACAGCGACTATCG
TCAACTGATGGAGGATTGTCAGGAGCTGCTGCGATGGGTAGCCTTGATTTGTGGGAAGAGTGAGGGGATCGACTATCAGG
GGCAACGTCTGGAACCGTGCCGGGAGGCACAACGGATTACGGTGCGTGATGCCTATGCCCGCTATGCTGTCAAGGATGTT
GCGGTCGCCGTGGTCGATGGAACCTTTGATGAACTGATGGTGACCCGAATCGAGTCTTCGCTGCCGACCCATGTACCGGT
AATCCTGATGGATTATCCCGCCGAAATGGCGTCCCTTGCCCGCCTGAAGGCCGATGACGAATCCGTTGCCGAGCGCTTTG
AGCTCTATCTGGGGGGGCTTGAGCTGGCCAATGGTTTTAGTGAGCTGAACGACCCCCGTGAACAACGGCAGCGTTTCCTG
GTGGCTAACGAGAGGAGGATAGAGGCTGGCCTGTCGCCCCTTCCCCTCCCCGAACCGTTCCTCGATGAACTGGCCTCCCT
GCCGCCATCGGCAGGGATCGCCCTGGGGATTGACCGGCTGGTGATGCTCTTCGCCGATGCTCCACGGATCGATGACGTAA
TCAGCTTCACGCCTGAGGAGCTGTAA

Upstream 100 bases:

>100_bases
GGGGTAAGGAGCGATTCAACGGAGCAGACCACACGCATCCATCCGGGGCGAGTCACGGTGACTCGCCCCTTTTTGCTTTA
CGCGACTTGACAGGATTAGC

Downstream 100 bases:

>100_bases
TTCAGAAAAGTATTCTGGCTTTTCTGTTTTGGATTTATGAATCTGCAACCGTTGTCAGATCCCGATACCTGACCATCTCC
CCCTGGTATGTCCTCAGAAT

Product: tRNA synthetase, class II (D, K and N)

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MHPRDHNLYLRADIVAAIRSFFASRRFLEVDTPHLIPANAPEEYIDPHGSGDRYLHTSPEICMKRLLCRGHERIFQICRC
WRRDERGSRHLPEFAMLEWYRRDSDYRQLMEDCQELLRWVALICGKSEGIDYQGQRLEPCREAQRITVRDAYARYAVKDV
AVAVVDGTFDELMVTRIESSLPTHVPVILMDYPAEMASLARLKADDESVAERFELYLGGLELANGFSELNDPREQRQRFL
VANERRIEAGLSPLPLPEPFLDELASLPPSAGIALGIDRLVMLFADAPRIDDVISFTPEEL

Sequences:

>Translated_301_residues
MHPRDHNLYLRADIVAAIRSFFASRRFLEVDTPHLIPANAPEEYIDPHGSGDRYLHTSPEICMKRLLCRGHERIFQICRC
WRRDERGSRHLPEFAMLEWYRRDSDYRQLMEDCQELLRWVALICGKSEGIDYQGQRLEPCREAQRITVRDAYARYAVKDV
AVAVVDGTFDELMVTRIESSLPTHVPVILMDYPAEMASLARLKADDESVAERFELYLGGLELANGFSELNDPREQRQRFL
VANERRIEAGLSPLPLPEPFLDELASLPPSAGIALGIDRLVMLFADAPRIDDVISFTPEEL
>Mature_301_residues
MHPRDHNLYLRADIVAAIRSFFASRRFLEVDTPHLIPANAPEEYIDPHGSGDRYLHTSPEICMKRLLCRGHERIFQICRC
WRRDERGSRHLPEFAMLEWYRRDSDYRQLMEDCQELLRWVALICGKSEGIDYQGQRLEPCREAQRITVRDAYARYAVKDV
AVAVVDGTFDELMVTRIESSLPTHVPVILMDYPAEMASLARLKADDESVAERFELYLGGLELANGFSELNDPREQRQRFL
VANERRIEAGLSPLPLPEPFLDELASLPPSAGIALGIDRLVMLFADAPRIDDVISFTPEEL

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=327, Percent_Identity=27.82874617737, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI194272210, Length=327, Percent_Identity=27.82874617737, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI87082379, Length=310, Percent_Identity=40.3225806451613, Blast_Score=194, Evalue=4e-51,
Organism=Escherichia coli, GI1789256, Length=318, Percent_Identity=33.9622641509434, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1790571, Length=317, Percent_Identity=32.1766561514196, Blast_Score=165, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI71994340, Length=338, Percent_Identity=26.9230769230769, Blast_Score=113, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17535925, Length=338, Percent_Identity=26.9230769230769, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17535927, Length=338, Percent_Identity=26.9230769230769, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320242, Length=326, Percent_Identity=29.1411042944785, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324256, Length=351, Percent_Identity=24.7863247863248, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640849, Length=327, Percent_Identity=25.9938837920489, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24640851, Length=327, Percent_Identity=25.9938837920489, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 34600; Mature: 34600

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPRDHNLYLRADIVAAIRSFFASRRFLEVDTPHLIPANAPEEYIDPHGSGDRYLHTSPE
CCCCCCCEEEHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHCCCCCCCCCEEECCHH
ICMKRLLCRGHERIFQICRCWRRDERGSRHLPEFAMLEWYRRDSDYRQLMEDCQELLRWV
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ALICGKSEGIDYQGQRLEPCREAQRITVRDAYARYAVKDVAVAVVDGTFDELMVTRIESS
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHC
LPTHVPVILMDYPAEMASLARLKADDESVAERFELYLGGLELANGFSELNDPREQRQRFL
CCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHH
VANERRIEAGLSPLPLPEPFLDELASLPPSAGIALGIDRLVMLFADAPRIDDVISFTPEE
HHCCHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCC
L
C
>Mature Secondary Structure
MHPRDHNLYLRADIVAAIRSFFASRRFLEVDTPHLIPANAPEEYIDPHGSGDRYLHTSPE
CCCCCCCEEEHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHCCCCCCCCCEEECCHH
ICMKRLLCRGHERIFQICRCWRRDERGSRHLPEFAMLEWYRRDSDYRQLMEDCQELLRWV
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ALICGKSEGIDYQGQRLEPCREAQRITVRDAYARYAVKDVAVAVVDGTFDELMVTRIESS
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHC
LPTHVPVILMDYPAEMASLARLKADDESVAERFELYLGGLELANGFSELNDPREQRQRFL
CCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHH
VANERRIEAGLSPLPLPEPFLDELASLPPSAGIALGIDRLVMLFADAPRIDDVISFTPEE
HHCCHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA