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The map label for this gene is pcnB [H]

Identifier: 118580041

GI number: 118580041

Start: 1734046

End: 1735413

Strand: Reverse

Name: pcnB [H]

Synonym: Ppro_1618

Alternate gene names: 118580041

Gene position: 1735413-1734046 (Counterclockwise)

Preceding gene: 118580042

Following gene: 118580039

Centisome position: 43.3

GC content: 60.23

Gene sequence:

>1368_bases
ATGAAAAAGAAAAAGAACTGCTCGGTGATCATCCCCCGGCAGGAGCATGAGATATCCCGTAAACTGGTGAGCCCGAACGC
CCTGCGCACCCTCTACCGTTTGAAAGACAACGGCTTTATCGGCTATCTCGTGGGCGGCTGTGTGCGCGACCTCCTGCTCG
GTCGAGAGCCGAAGGATTTCGACGTGGTAACCAATGCCACCCCTAACCAGGTGAAGCGGATATTCCGCAACTGCCGCCTT
GTCGGGAGACGCTTTCGTCTTGCGCACATCCACTTCCAGGATGAGATCATCGAGGTGGCGACCTTTCGCTCCCCGGCTTC
GGACGAGCCGGATCCCGCGCCGAAAAAACCAGACGAGACGGAGCAGCGACACCCGAGGATGTTGAAGGACGATGACGGCA
TGATCCTGCGGGACAATGTCTACGGCACCCCCGAAGAGGATGCCCTGCGCCGCGACTTCACCGTCAACTCGCTTGCCTAC
AATATTGCCGACTTCTCGATCATTGACTTCACCGGCGGCCTCGCCGACCTTAGTGCCGGCATCATTCGCACCATCGGCGA
TCCCGCCGTCCGTTTCCAGGAAGACCCGGTACGGATGCTGCGAGCGGTGCGCTTTGCCGCCCAGCTCGGCTTCACCATCG
AGGAACAGACCCGGAAGGCGCTGGTCGCCGCCACGGATACCATTGTCAGGTCTGCGCCGCCCCGCCTCTACGAAGAGGTG
CTCAAGCTGTTCCTCTCGGGAGAAGGAGCCAAGTGCTACGATCACCTCAGCCGGACCGGACTCTTTGCGGCGCTTTTCCC
GGATTTTTCGAACTGGCTCGGCGTCGAAGGCGAGTGTTTTCCCCACACCCGCTTCGGGGAAATGCTCGCTCACGTCGATT
CGCGCATCCAGCAGGGTCACAAAGTCTCCCCGCCACTTCTCCTGGCACTCCTTTTCGGCGAGTACATCGATGACAAGGCG
GAGCGTTTTCGCCGCCAGAATATGCCTTGGCAGCAGAGCGTTAACGCCGCGGTCGCGGAGTTCATGGGCGAAACCTGCCC
GATCGTCATGATCGCCAACCGAGTCGGGATCGCACTCCGCGACATCATCAGCCAGCAGATGCGCCTGAAAAAGATCCCCG
GCCGTCGACCGGAGTCGCTCATCGCCCGTAACGATTTCAGTGATATCATCGAATACTGCCGGATCACCCGCGGCAACAGG
AAGGATATCGTCAAACAGCTGGACTGGTGGGAAACTCGGGCAGGGCAGCTGCCTCAGACGCCGACGGCGGACGAACCTGC
CGAAGAAACCGGGGCACCGCAACGAAAGAGAAAGAGACGCCGGCGACGACGAAACCCTGATGCGAAAAGCACGACGGAAG
GGGCATAG

Upstream 100 bases:

>100_bases
AGGGAGGAAGCAGCATAGTATTTACGCCTCCGGAATTGCGGCCGATCAATAACAGCTCAAAGGCATGACAACACATGAAT
GCACTGGTGGCGATAACAGG

Downstream 100 bases:

>100_bases
CTGAAGGGGTGTCAAGGCAGGGGAGTGGCGGCGGCGAGAACAGCTCCTCGCACCGCTGTCCAGGTTATGTCGTGTGGCTC
CAGGCAGCACGCCCCATCCT

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKKKKNCSVIIPRQEHEISRKLVSPNALRTLYRLKDNGFIGYLVGGCVRDLLLGREPKDFDVVTNATPNQVKRIFRNCRL
VGRRFRLAHIHFQDEIIEVATFRSPASDEPDPAPKKPDETEQRHPRMLKDDDGMILRDNVYGTPEEDALRRDFTVNSLAY
NIADFSIIDFTGGLADLSAGIIRTIGDPAVRFQEDPVRMLRAVRFAAQLGFTIEEQTRKALVAATDTIVRSAPPRLYEEV
LKLFLSGEGAKCYDHLSRTGLFAALFPDFSNWLGVEGECFPHTRFGEMLAHVDSRIQQGHKVSPPLLLALLFGEYIDDKA
ERFRRQNMPWQQSVNAAVAEFMGETCPIVMIANRVGIALRDIISQQMRLKKIPGRRPESLIARNDFSDIIEYCRITRGNR
KDIVKQLDWWETRAGQLPQTPTADEPAEETGAPQRKRKRRRRRRNPDAKSTTEGA

Sequences:

>Translated_455_residues
MKKKKNCSVIIPRQEHEISRKLVSPNALRTLYRLKDNGFIGYLVGGCVRDLLLGREPKDFDVVTNATPNQVKRIFRNCRL
VGRRFRLAHIHFQDEIIEVATFRSPASDEPDPAPKKPDETEQRHPRMLKDDDGMILRDNVYGTPEEDALRRDFTVNSLAY
NIADFSIIDFTGGLADLSAGIIRTIGDPAVRFQEDPVRMLRAVRFAAQLGFTIEEQTRKALVAATDTIVRSAPPRLYEEV
LKLFLSGEGAKCYDHLSRTGLFAALFPDFSNWLGVEGECFPHTRFGEMLAHVDSRIQQGHKVSPPLLLALLFGEYIDDKA
ERFRRQNMPWQQSVNAAVAEFMGETCPIVMIANRVGIALRDIISQQMRLKKIPGRRPESLIARNDFSDIIEYCRITRGNR
KDIVKQLDWWETRAGQLPQTPTADEPAEETGAPQRKRKRRRRRRNPDAKSTTEGA
>Mature_455_residues
MKKKKNCSVIIPRQEHEISRKLVSPNALRTLYRLKDNGFIGYLVGGCVRDLLLGREPKDFDVVTNATPNQVKRIFRNCRL
VGRRFRLAHIHFQDEIIEVATFRSPASDEPDPAPKKPDETEQRHPRMLKDDDGMILRDNVYGTPEEDALRRDFTVNSLAY
NIADFSIIDFTGGLADLSAGIIRTIGDPAVRFQEDPVRMLRAVRFAAQLGFTIEEQTRKALVAATDTIVRSAPPRLYEEV
LKLFLSGEGAKCYDHLSRTGLFAALFPDFSNWLGVEGECFPHTRFGEMLAHVDSRIQQGHKVSPPLLLALLFGEYIDDKA
ERFRRQNMPWQQSVNAAVAEFMGETCPIVMIANRVGIALRDIISQQMRLKKIPGRRPESLIARNDFSDIIEYCRITRGNR
KDIVKQLDWWETRAGQLPQTPTADEPAEETGAPQRKRKRRRRRRNPDAKSTTEGA

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=405, Percent_Identity=41.2345679012346, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1789436, Length=244, Percent_Identity=30.7377049180328, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=185, Percent_Identity=32.4324324324324, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281360112, Length=230, Percent_Identity=28.695652173913, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21357337, Length=230, Percent_Identity=28.695652173913, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 51852; Mature: 51852

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKKNCSVIIPRQEHEISRKLVSPNALRTLYRLKDNGFIGYLVGGCVRDLLLGREPKDF
CCCCCCCCEEECCHHHHHHHHHCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCCCC
DVVTNATPNQVKRIFRNCRLVGRRFRLAHIHFQDEIIEVATFRSPASDEPDPAPKKPDET
CEECCCCHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
EQRHPRMLKDDDGMILRDNVYGTPEEDALRRDFTVNSLAYNIADFSIIDFTGGLADLSAG
HHHCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCHHHHHHHHHCEEEEECCCCHHHHHHH
IIRTIGDPAVRFQEDPVRMLRAVRFAAQLGFTIEEQTRKALVAATDTIVRSAPPRLYEEV
HHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LKLFLSGEGAKCYDHLSRTGLFAALFPDFSNWLGVEGECFPHTRFGEMLAHVDSRIQQGH
HHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC
KVSPPLLLALLFGEYIDDKAERFRRQNMPWQQSVNAAVAEFMGETCPIVMIANRVGIALR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHH
DIISQQMRLKKIPGRRPESLIARNDFSDIIEYCRITRGNRKDIVKQLDWWETRAGQLPQT
HHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
PTADEPAEETGAPQRKRKRRRRRRNPDAKSTTEGA
CCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKKKKNCSVIIPRQEHEISRKLVSPNALRTLYRLKDNGFIGYLVGGCVRDLLLGREPKDF
CCCCCCCCEEECCHHHHHHHHHCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCCCC
DVVTNATPNQVKRIFRNCRLVGRRFRLAHIHFQDEIIEVATFRSPASDEPDPAPKKPDET
CEECCCCHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
EQRHPRMLKDDDGMILRDNVYGTPEEDALRRDFTVNSLAYNIADFSIIDFTGGLADLSAG
HHHCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCHHHHHHHHHCEEEEECCCCHHHHHHH
IIRTIGDPAVRFQEDPVRMLRAVRFAAQLGFTIEEQTRKALVAATDTIVRSAPPRLYEEV
HHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LKLFLSGEGAKCYDHLSRTGLFAALFPDFSNWLGVEGECFPHTRFGEMLAHVDSRIQQGH
HHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC
KVSPPLLLALLFGEYIDDKAERFRRQNMPWQQSVNAAVAEFMGETCPIVMIANRVGIALR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHH
DIISQQMRLKKIPGRRPESLIARNDFSDIIEYCRITRGNRKDIVKQLDWWETRAGQLPQT
HHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
PTADEPAEETGAPQRKRKRRRRRRNPDAKSTTEGA
CCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]