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The map label for this gene is gcvH [H]

Identifier: 118580014

GI number: 118580014

Start: 1699628

End: 1700002

Strand: Direct

Name: gcvH [H]

Synonym: Ppro_1591

Alternate gene names: 118580014

Gene position: 1699628-1700002 (Clockwise)

Preceding gene: 118580013

Following gene: 118580015

Centisome position: 42.41

GC content: 58.13

Gene sequence:

>375_bases
ATGGCGGAGATCTATTTCACCAAAGAACATGAGTGGGTCAGGCTGCAGGACGGGGTGGGGGTAGTCGGCATAAGCGAACA
TGCGGCCCAGGAGTTGGGCGATGTCACCTTCGTTGAACTGCCCAAGATTGGAGTAAAGGTCGGACAGTTCGATCTGCTGG
GAAGCATCGAGTCGGTCAAGGCTGCCAGTGATATCTTCGCGCCGGTTTCCGGAACCGTGGTCAAGGTCAACGGGGAGCTG
GAGGGCGCGCCCGAACTGGTCAACGAGAGCCCCTGGGACAGGGGGTGGATGGCCTGGCTGGAACTGGATGACGCAACCGA
ACTGGAGAAACTGATGACGGAAGAACAGTACGCCTGCTACCTGAAAAGCCTGTAG

Upstream 100 bases:

>100_bases
GCGAGAACAGGGGAGGCTCCTTGAGAGTTTACGTTTGTCGATTATGATTATGAGAGTCATTTGAATGAGCTGCATGAGAT
CGGGAAGGGGAGGGGAGATC

Downstream 100 bases:

>100_bases
GAGTCAGCCATGGATGACAGCCATTATTGTCCCCACACGCCGGAGGAGAGGGGGGAGATGCTCGACCTGATCGGCGTCGC
CAGTGTGGATGATCTGTTCG

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 123

Protein sequence:

>124_residues
MAEIYFTKEHEWVRLQDGVGVVGISEHAAQELGDVTFVELPKIGVKVGQFDLLGSIESVKAASDIFAPVSGTVVKVNGEL
EGAPELVNESPWDRGWMAWLELDDATELEKLMTEEQYACYLKSL

Sequences:

>Translated_124_residues
MAEIYFTKEHEWVRLQDGVGVVGISEHAAQELGDVTFVELPKIGVKVGQFDLLGSIESVKAASDIFAPVSGTVVKVNGEL
EGAPELVNESPWDRGWMAWLELDDATELEKLMTEEQYACYLKSL
>Mature_123_residues
AEIYFTKEHEWVRLQDGVGVVGISEHAAQELGDVTFVELPKIGVKVGQFDLLGSIESVKAASDIFAPVSGTVVKVNGELE
GAPELVNESPWDRGWMAWLELDDATELEKLMTEEQYACYLKSL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=45.3781512605042, Blast_Score=119, Evalue=6e-28,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=45.3781512605042, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789271, Length=122, Percent_Identity=48.3606557377049, Blast_Score=115, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=117, Percent_Identity=52.1367521367521, Blast_Score=132, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=47.8260869565217, Blast_Score=124, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6319272, Length=122, Percent_Identity=43.4426229508197, Blast_Score=105, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17865652, Length=122, Percent_Identity=46.7213114754098, Blast_Score=118, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13701; Mature: 13569

Theoretical pI: Translated: 4.02; Mature: 4.02

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIYFTKEHEWVRLQDGVGVVGISEHAAQELGDVTFVELPKIGVKVGQFDLLGSIESVK
CCEEEEECCCCEEEECCCCEEEEECHHHHHHHCCEEEEECCCCCEEECCEEECCCHHHHH
AASDIFAPVSGTVVKVNGELEGAPELVNESPWDRGWMAWLELDDATELEKLMTEEQYACY
HHHHHCCCCCCEEEEECCCCCCCCHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHH
LKSL
CCCC
>Mature Secondary Structure 
AEIYFTKEHEWVRLQDGVGVVGISEHAAQELGDVTFVELPKIGVKVGQFDLLGSIESVK
CEEEEECCCCEEEECCCCEEEEECHHHHHHHCCEEEEECCCCCEEECCEEECCCHHHHH
AASDIFAPVSGTVVKVNGELEGAPELVNESPWDRGWMAWLELDDATELEKLMTEEQYACY
HHHHHCCCCCCEEEEECCCCCCCCHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHH
LKSL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA