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The map label for this gene is recN [H]

Identifier: 118580001

GI number: 118580001

Start: 1683129

End: 1684799

Strand: Reverse

Name: recN [H]

Synonym: Ppro_1578

Alternate gene names: 118580001

Gene position: 1684799-1683129 (Counterclockwise)

Preceding gene: 118580002

Following gene: 118580000

Centisome position: 42.04

GC content: 65.11

Gene sequence:

>1671_bases
ATGCTGACCGACCTGTCCATCACCAATATAGCCATCATCGATTCGCTGCACCTCTCCCTCAAACCGGGCCTGACCATCCT
GACCGGCGAGACCGGGGCCGGCAAATCGATCATCATCGACGCCGTGGGGCTGATCATGGGGGGACGCGCCTCGGCCGATC
TGATCCGTTCCGGCTCGGACGAGGCCGTGGTGGAGGCAATCTTCGACATCTCGGATATGTCCGAGCTGTCGCAGCTGCTC
AGCGACTCCGGTTTCCCCTGCGAAGCTGAACTGCTGGTCAAACGCTCCATCTCCCGCGCCGGAAAGAACCGCATCTTCAT
CAACGGCGCCATGGCAACCCTGACCCTGCTCTCCGACATCTCCCGCCGCCTGATCAACATCTATGGACAGCATGAATCCC
AGACCCTGCTCAGACCGGAGAACCAGCTGCTGCTCCTGGACCTCTTTGCCGGAAACACTGATCTGCGCGGAGCTTTCGCC
AGCCTGTATGCAAAGCTGCAGACAGTACGGGAGCGCCTAGCCCATGCGGACGAACGGGAGCGCGAGGCGGCCCGCAGGCT
GGACCTGCTGTCGTTCCAGTCCCAGGAGATAGCCGGGGCAGAGCTTCGGGACGGTGAGGAGGAGCAGCTGGAGGAAGAAC
GCCAGCTGCTGGCCAACGCGGAGAAACTGGGGGGAGCCAGCGCCGAGGCGTTCGAACTGCTCTACGGCGGCGATGGCGCC
CTTCTGGGTCAGCTGCGCAGGATAAGCAACGCCATCCGCGAAATCGCCGCCATCGACCAGACCCTGGGGGAGACGGCAAC
CTCCTTGGAAAACTGCTACCTGCAGCTGGAGGACGCCGCCATGACCCTGCGCGGCTATGCCTCCCGGGTCGAGGCGGACC
CGCTCTCCCTGCAACGCGTCGAGGATCGTCTGGACCAGATCAACCGACTCAAGCGCAAGTACGGCCCGACCATCCGGGAC
ATCCTGGAGTTCCAACAACAGGTCGACGGAGAGCTGGAACAGATCCGCGACCAGGAGCATGACCGGGAGGCGCTGCAGGG
CCAGTGCGACCGGCTGGAGAAGGAGCTGCGCCGCTGCGGAGCGGAACTGAGCCGACAACGCGCGGCCGCGGCGGACGCGT
TGAAGCAGGCCCTGGAAAACGAGGCGCGCCAGCTTGCCATGAAGAACGCCGTCATCGAGGCCGACCTGACCCTGCTGGAC
GAACCGCGCCCCACCGGATTCGAGCGCATGGAGCTGCTCTTCTCCCCCAACGCCGGAGAGCCGCCGCGCCCCCTGGCAAA
AATCGCCTCGGGCGGCGAACTGTCGCGCCTGATGCTGGCCTTCAAGCAGGTCCTGCCCGAGGGGGACGTTCCCACCCTGG
TATTCGACGAGGTGGACAGCGGCATCGGTGGCGCCACCTCGGAAACCGTGGGCAGGAAGCTGAAGAGGGTTGCCCGGCGC
CAGCAGGTGCTTTGTATCACCCACCTCCCCCAGGTTGCGGTGTTCGCCGACCAGCATCTGCACGTTGCCAAGCGGGTCGA
CAACGGCCGCACCGCCACAGCCGTCACCCCCCTGGACAAGAAAGCCCGCATCGAGGAGATCTCCCGCATGCTGGGAGGCG
CAGTCATCACCGACACCACCCGCAGCCACGCCGGCGAGCTGCTGGCAACGGCCAGGAGTTCATCACCATGA

Upstream 100 bases:

>100_bases
ATAAATCGGTTCAATGCTCGCGGTTCAGGGTTCATGGTTGACGTCACTCATACCTCTGAACGTTGAACCTCGAACCACGA
ATCACATACCAGGTGTTATT

Downstream 100 bases:

>100_bases
CCGCTGCCATCACTATCGGCGTCAGCGCCTGTCTGCTGGGCCAGGAGGTGCGCAACGACGGCGGCCATTAGCATGACCGC
TACCTCACCGACACCCTGGG

Product: DNA repair protein RecN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MLTDLSITNIAIIDSLHLSLKPGLTILTGETGAGKSIIIDAVGLIMGGRASADLIRSGSDEAVVEAIFDISDMSELSQLL
SDSGFPCEAELLVKRSISRAGKNRIFINGAMATLTLLSDISRRLINIYGQHESQTLLRPENQLLLLDLFAGNTDLRGAFA
SLYAKLQTVRERLAHADEREREAARRLDLLSFQSQEIAGAELRDGEEEQLEEERQLLANAEKLGGASAEAFELLYGGDGA
LLGQLRRISNAIREIAAIDQTLGETATSLENCYLQLEDAAMTLRGYASRVEADPLSLQRVEDRLDQINRLKRKYGPTIRD
ILEFQQQVDGELEQIRDQEHDREALQGQCDRLEKELRRCGAELSRQRAAAADALKQALENEARQLAMKNAVIEADLTLLD
EPRPTGFERMELLFSPNAGEPPRPLAKIASGGELSRLMLAFKQVLPEGDVPTLVFDEVDSGIGGATSETVGRKLKRVARR
QQVLCITHLPQVAVFADQHLHVAKRVDNGRTATAVTPLDKKARIEEISRMLGGAVITDTTRSHAGELLATARSSSP

Sequences:

>Translated_556_residues
MLTDLSITNIAIIDSLHLSLKPGLTILTGETGAGKSIIIDAVGLIMGGRASADLIRSGSDEAVVEAIFDISDMSELSQLL
SDSGFPCEAELLVKRSISRAGKNRIFINGAMATLTLLSDISRRLINIYGQHESQTLLRPENQLLLLDLFAGNTDLRGAFA
SLYAKLQTVRERLAHADEREREAARRLDLLSFQSQEIAGAELRDGEEEQLEEERQLLANAEKLGGASAEAFELLYGGDGA
LLGQLRRISNAIREIAAIDQTLGETATSLENCYLQLEDAAMTLRGYASRVEADPLSLQRVEDRLDQINRLKRKYGPTIRD
ILEFQQQVDGELEQIRDQEHDREALQGQCDRLEKELRRCGAELSRQRAAAADALKQALENEARQLAMKNAVIEADLTLLD
EPRPTGFERMELLFSPNAGEPPRPLAKIASGGELSRLMLAFKQVLPEGDVPTLVFDEVDSGIGGATSETVGRKLKRVARR
QQVLCITHLPQVAVFADQHLHVAKRVDNGRTATAVTPLDKKARIEEISRMLGGAVITDTTRSHAGELLATARSSSP
>Mature_556_residues
MLTDLSITNIAIIDSLHLSLKPGLTILTGETGAGKSIIIDAVGLIMGGRASADLIRSGSDEAVVEAIFDISDMSELSQLL
SDSGFPCEAELLVKRSISRAGKNRIFINGAMATLTLLSDISRRLINIYGQHESQTLLRPENQLLLLDLFAGNTDLRGAFA
SLYAKLQTVRERLAHADEREREAARRLDLLSFQSQEIAGAELRDGEEEQLEEERQLLANAEKLGGASAEAFELLYGGDGA
LLGQLRRISNAIREIAAIDQTLGETATSLENCYLQLEDAAMTLRGYASRVEADPLSLQRVEDRLDQINRLKRKYGPTIRD
ILEFQQQVDGELEQIRDQEHDREALQGQCDRLEKELRRCGAELSRQRAAAADALKQALENEARQLAMKNAVIEADLTLLD
EPRPTGFERMELLFSPNAGEPPRPLAKIASGGELSRLMLAFKQVLPEGDVPTLVFDEVDSGIGGATSETVGRKLKRVARR
QQVLCITHLPQVAVFADQHLHVAKRVDNGRTATAVTPLDKKARIEEISRMLGGAVITDTTRSHAGELLATARSSSP

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=553, Percent_Identity=36.3471971066908, Blast_Score=328, Evalue=6e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 60896; Mature: 60896

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTDLSITNIAIIDSLHLSLKPGLTILTGETGAGKSIIIDAVGLIMGGRASADLIRSGSD
CCCCCCCCHHHHHHHHHEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
EAVVEAIFDISDMSELSQLLSDSGFPCEAELLVKRSISRAGKNRIFINGAMATLTLLSDI
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
SRRLINIYGQHESQTLLRPENQLLLLDLFAGNTDLRGAFASLYAKLQTVRERLAHADERE
HHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
REAARRLDLLSFQSQEIAGAELRDGEEEQLEEERQLLANAEKLGGASAEAFELLYGGDGA
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHH
LLGQLRRISNAIREIAAIDQTLGETATSLENCYLQLEDAAMTLRGYASRVEADPLSLQRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCHHHHHH
EDRLDQINRLKRKYGPTIRDILEFQQQVDGELEQIRDQEHDREALQGQCDRLEKELRRCG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
AELSRQRAAAADALKQALENEARQLAMKNAVIEADLTLLDEPRPTGFERMELLFSPNAGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHEEECCCCCC
PPRPLAKIASGGELSRLMLAFKQVLPEGDVPTLVFDEVDSGIGGATSETVGRKLKRVARR
CCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHHH
QQVLCITHLPQVAVFADQHLHVAKRVDNGRTATAVTPLDKKARIEEISRMLGGAVITDTT
HHEEHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCEEEECCC
RSHAGELLATARSSSP
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLTDLSITNIAIIDSLHLSLKPGLTILTGETGAGKSIIIDAVGLIMGGRASADLIRSGSD
CCCCCCCCHHHHHHHHHEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
EAVVEAIFDISDMSELSQLLSDSGFPCEAELLVKRSISRAGKNRIFINGAMATLTLLSDI
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
SRRLINIYGQHESQTLLRPENQLLLLDLFAGNTDLRGAFASLYAKLQTVRERLAHADERE
HHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
REAARRLDLLSFQSQEIAGAELRDGEEEQLEEERQLLANAEKLGGASAEAFELLYGGDGA
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHH
LLGQLRRISNAIREIAAIDQTLGETATSLENCYLQLEDAAMTLRGYASRVEADPLSLQRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCHHHHHH
EDRLDQINRLKRKYGPTIRDILEFQQQVDGELEQIRDQEHDREALQGQCDRLEKELRRCG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
AELSRQRAAAADALKQALENEARQLAMKNAVIEADLTLLDEPRPTGFERMELLFSPNAGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHEEECCCCCC
PPRPLAKIASGGELSRLMLAFKQVLPEGDVPTLVFDEVDSGIGGATSETVGRKLKRVARR
CCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHHH
QQVLCITHLPQVAVFADQHLHVAKRVDNGRTATAVTPLDKKARIEEISRMLGGAVITDTT
HHEEHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCEEEECCC
RSHAGELLATARSSSP
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]