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The map label for this gene is mtbC3 [H]

Identifier: 118579855

GI number: 118579855

Start: 1530627

End: 1531295

Strand: Reverse

Name: mtbC3 [H]

Synonym: Ppro_1431

Alternate gene names: 118579855

Gene position: 1531295-1530627 (Counterclockwise)

Preceding gene: 118579857

Following gene: 118579854

Centisome position: 38.21

GC content: 59.34

Gene sequence:

>669_bases
ATGGAAAACAGATATCTGAACGACTATTTCGACGCCGTTTTCGATACCGACCGTGATCGGGCTTTAGAAGTTATCCGGCA
GGCCTTGGATGGTGGCGTTACCCCCGAAGAGGTCATTTTCGGGATCATCGTCCCCTCCATGGAGAAGATGATCGGCGGTA
TGGTGACCGATTCGCTGGTTACCCTCTCCCAGCACTTCCTGGCGTCCCAGATTGCCGAAGAGGTCACCGACATGCTGATT
CCCCGCTTTGCAACCGCGCCGGAGATGCAGGGGCATGTGATAATCGGCAGCAGTTTCGGCGATTTCCATGGTCTTGGGAA
GAAGATCGTGGGGGGCTGCCTGAGAGCCAAGATGTTTCAGGTGACCGACCTGGGAATCAATGTCCGCCCTGAGCGTTTCG
TGGAGGCGGCGGTGAACGGTGGGGCCCAGGTGATCAGCGTATCCTCCATGATGGTCCACACCGCCCTGGGGGATATGGGT
TCCAGACGAGTACGGCAGCTGCTCCGTGAGGGGGGGCTGGAAGAGCGGATCAAGCTGATAGTGGGAGGCGCCCCCTACCG
CTACGATGAGCAACTCTACCTCCAGGCGGGGGCAGATGCCTGGGCGGCAACGGCAGTGGAGGGGGCGGAGATCATCTCCC
GCCTGATCAAGGAGGTACGTGCGCAATGA

Upstream 100 bases:

>100_bases
AACGGGAATAAGGCGCCGGTTTGGCTTGTCGGCCGATTTTAGCTATACTCCTGATACTCCGGTTACATGCCGGCTTTATT
CGCCTATGCAGGAATTTATA

Downstream 100 bases:

>100_bases
CCGGAATGCAGCGGTTCCAGGCAATCATGGCCGGCCAGCTCCCCGATCGGGTACCAGTCCTGTGCAATCTGCTGGACCAG
GGAGCCCGTGAGCTCGGTAT

Product: cobalamin B12-binding domain-containing protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MENRYLNDYFDAVFDTDRDRALEVIRQALDGGVTPEEVIFGIIVPSMEKMIGGMVTDSLVTLSQHFLASQIAEEVTDMLI
PRFATAPEMQGHVIIGSSFGDFHGLGKKIVGGCLRAKMFQVTDLGINVRPERFVEAAVNGGAQVISVSSMMVHTALGDMG
SRRVRQLLREGGLEERIKLIVGGAPYRYDEQLYLQAGADAWAATAVEGAEIISRLIKEVRAQ

Sequences:

>Translated_222_residues
MENRYLNDYFDAVFDTDRDRALEVIRQALDGGVTPEEVIFGIIVPSMEKMIGGMVTDSLVTLSQHFLASQIAEEVTDMLI
PRFATAPEMQGHVIIGSSFGDFHGLGKKIVGGCLRAKMFQVTDLGINVRPERFVEAAVNGGAQVISVSSMMVHTALGDMG
SRRVRQLLREGGLEERIKLIVGGAPYRYDEQLYLQAGADAWAATAVEGAEIISRLIKEVRAQ
>Mature_222_residues
MENRYLNDYFDAVFDTDRDRALEVIRQALDGGVTPEEVIFGIIVPSMEKMIGGMVTDSLVTLSQHFLASQIAEEVTDMLI
PRFATAPEMQGHVIIGSSFGDFHGLGKKIVGGCLRAKMFQVTDLGINVRPERFVEAAVNGGAQVISVSSMMVHTALGDMG
SRRVRQLLREGGLEERIKLIVGGAPYRYDEQLYLQAGADAWAATAVEGAEIISRLIKEVRAQ

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 24303; Mature: 24303

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENRYLNDYFDAVFDTDRDRALEVIRQALDGGVTPEEVIFGIIVPSMEKMIGGMVTDSLV
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TLSQHFLASQIAEEVTDMLIPRFATAPEMQGHVIIGSSFGDFHGLGKKIVGGCLRAKMFQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
VTDLGINVRPERFVEAAVNGGAQVISVSSMMVHTALGDMGSRRVRQLLREGGLEERIKLI
HHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEE
VGGAPYRYDEQLYLQAGADAWAATAVEGAEIISRLIKEVRAQ
ECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENRYLNDYFDAVFDTDRDRALEVIRQALDGGVTPEEVIFGIIVPSMEKMIGGMVTDSLV
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TLSQHFLASQIAEEVTDMLIPRFATAPEMQGHVIIGSSFGDFHGLGKKIVGGCLRAKMFQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
VTDLGINVRPERFVEAAVNGGAQVISVSSMMVHTALGDMGSRRVRQLLREGGLEERIKLI
HHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEE
VGGAPYRYDEQLYLQAGADAWAATAVEGAEIISRLIKEVRAQ
ECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} 0.035 {S-methyltetrahydrofolate}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]