| Definition | Pelobacter propionicus DSM 2379 chromosome, complete genome. |
|---|---|
| Accession | NC_008609 |
| Length | 4,008,000 |
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The map label for this gene is metH [H]
Identifier: 118579837
GI number: 118579837
Start: 1508159
End: 1510579
Strand: Direct
Name: metH [H]
Synonym: Ppro_1413
Alternate gene names: 118579837
Gene position: 1508159-1510579 (Clockwise)
Preceding gene: 118579836
Following gene: 118579838
Centisome position: 37.63
GC content: 65.55
Gene sequence:
>2421_bases ATGACACCATTTCTCGAGGCGATCCAGGAGAGGGTGCTGATTCTGGACGGGGCCATGGGTACCATGCTTCAGGAGCGGGG GCTTCGGCCGGGCCAGTCTCCCGAAGAACTCAACCTGACCATGCCCGATGTGGTGGCGTCGGTCCACCGTGAGTATATCG AGGCTGGCGCCGACATCATCATCACCAACAGCTTCGGCGGATCGCGCTTCAAGCTGGCCCACTTTGGGCTGGAAGGGCGC CTGGCCGAGATCAACGCCCGCGCCGTGGAGATTGCCCGTGGCGAGGCGCGGGGCAGGGCCTATGTGGGCGGCTCCATGGG TCCCACCGGTCAGTTCGTGGAGCCCTTGGGTGAGGTTTCCTTCGACCGTATGAAAGAATCCTTCCGTGAGCAGGCCGAGG CGCTGGTCTCGGCCGGGGTCGATCTGATCAGCCTGGAGACCTTCCTGGACATCAAGGAGTGCCGCGCAGCGGTGATCGCC ATCCGCGAGGTCTCGCCGACCATCCCGATCATCGCCATGATGACTTTCGATGACAACGGCCGTTCCGTATTGGGAACGCC GCCCGAGGCCGCCGCCATCACCCTGGCCGCTGCTGGCGTCGATATCGTCGGCTCCAACTGCGGGCTGGGGGTGGAGGGGA TCCACGACATCCTGGTGCGCATGCGCCGGGTGACCTCCCTGCCGCTCATCTCCCAGGCCAACGCGGGGCTGCCGATCCTG AAAGACGGAATCACCGTCTTCCCCGGAACCCCACGGGAGATGACCGACTACCACGACCGGCTGATCAGCCTGGGGGTGCG GGTGATCGGCGGCTGCTGCGGCACCACGCCGGCACATATCAGGGCCATGAAGGAGGCCCTTGCCGGCCGCCAGCAGGTTT GGCAGGAACCGACTCACCTGGAAGGAATGACCCTGCTCTCCTGCCGGGGCGGGTGGACCGCCGTGGGGCCCGGCCAGAAG ACCGCCATCATCGGCGAACGTATCAACCCCACCGGCAAGAAGCTCTACTCCAAGGAACTGCAGGAGGGAAAGGTCTCCTA CATCCGCCGCGAGGCCATGGAGCAGGCGCAGTTGGGGGCCACGTTGCTGGACCTGAACGTGGGGGCGCCGGGGATCGACG AGCCGGTTGCCATGGAGCGGGCCGTGTTCTGCGCCAGCGGGGCAGTCGGCGTGCCGCTGGTGCTGGATTCCTCCAGCCCT GCCGCCCTGGAGGCGGGGCTCAAGGCGGCCGACGGCAAGGTGCTGATCAACTCCGTCTCCGGCGAGGCCAAGAGCCTCTC CCGGGTGCTGCCACTGGCCAAGAAGTACGGTGCAGCCCTGATCGGCCTGACCCTGGATGCCAAGGGGATACCGGACAGCG CCGAGGGGAGGCTGGCCATCGCCCGCAGGATCCGCAACGCCGCCCGGCGGCAGGGGATTCCTGACCAGGACATCATCATC GACTGCCTGACCCTGACGGTCAGCGCCGAGCAGAAACGGGCGGCCGAGACCCTGCGCACCATCCGTCTGGTCAAGGAGAA GCTGGGGCTCTCCACGGTGCTGGGGGTCAGCAACATCTCTTTTGGCCTGCCCCAGCGGCCGCTGATCTCGTCCTCGTTCT TCTCCATGGCCATGGCCGCCGGTCTGGATGCGGCCATCATCAACCCAAGGGAAAAGGCCATGATGGATGCCTGGCGTTCG GCCATGGTGCTGCTCAACCGCGACCCACGGGCCGAAGCATTCATCCAGACCTATCGGGACGAACAGGCGGTTGCCACGCC CGCCGCCCCTGTTTCGGACGCGCCCCAGGAGATCCGGCAGCGGCTGGGCCAGGCGGTGATCAACGGCGACAGGGACGGCA TCATCGGGCTGGTGGAAGAGGCTCTGGCCCAGGGGCTGGCCCCGCTGCAGATCAGCAACGAAGGATTCCTCCCCGGCCTG GAGGAGGTGGGGCGGCGTTTCGAGAAGAACATCTTCTTCCTCCCCCAGGTGATGCAGTCGGCCGATACCATGCACGCCGG TTTCGCCCGCCTCAAGGAGGAGATGAAGGGGCAGCCTCTGGAGAGCCGCGGCCGAATCCTGATGGCCACCGTGGAGGGGG ACATCCACGATATCGGCAAGAACATCGTCTGCACCCTGCTGGAGAATCACGGCTTTGAGGTGTTCGACATCGGCAAGAAC GTGTCTGCCGCCACCATCGTCGCCAAGGCCAGGGAGTTCGGGGTGGATGCTGTGGGTCTCTCGGCGCTGATGACCACCAC CATGGCCGAGATGGACACGGTCCTCAAGAAGCTCAAGGCGGTGGGGATCAAGGCATTCACCATGATCGGCGGAGCTGTGG TGACCCAGGAGTACGCCGACCGCATCGGCGCCGATATCTACGCCCGTGACGCCATGGAGGCGGTGGCCAAGGTCAAGAAA CTGCTCCAGATAGCCGATTAG
Upstream 100 bases:
>100_bases ACCTGGACCTGTCCGCACCGCTGACCGCCGACTATGGGCTGGAGGGGCCGCCCTCCGATGCCGCGAAACCATCACATACA GACACCACAGGGGGTACACC
Downstream 100 bases:
>100_bases CTCTCTGTTGCCTGCCGGGAAGGTTTTTTCCCGCTTACCAGGTTCCTGCTGCGCTACAGTAAACAAACAACGGTGACTCG TACCTGGTCTCCAGTCGCCA
Product: homocysteine S-methyltransferase
Products: NA
Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]
Number of amino acids: Translated: 806; Mature: 805
Protein sequence:
>806_residues MTPFLEAIQERVLILDGAMGTMLQERGLRPGQSPEELNLTMPDVVASVHREYIEAGADIIITNSFGGSRFKLAHFGLEGR LAEINARAVEIARGEARGRAYVGGSMGPTGQFVEPLGEVSFDRMKESFREQAEALVSAGVDLISLETFLDIKECRAAVIA IREVSPTIPIIAMMTFDDNGRSVLGTPPEAAAITLAAAGVDIVGSNCGLGVEGIHDILVRMRRVTSLPLISQANAGLPIL KDGITVFPGTPREMTDYHDRLISLGVRVIGGCCGTTPAHIRAMKEALAGRQQVWQEPTHLEGMTLLSCRGGWTAVGPGQK TAIIGERINPTGKKLYSKELQEGKVSYIRREAMEQAQLGATLLDLNVGAPGIDEPVAMERAVFCASGAVGVPLVLDSSSP AALEAGLKAADGKVLINSVSGEAKSLSRVLPLAKKYGAALIGLTLDAKGIPDSAEGRLAIARRIRNAARRQGIPDQDIII DCLTLTVSAEQKRAAETLRTIRLVKEKLGLSTVLGVSNISFGLPQRPLISSSFFSMAMAAGLDAAIINPREKAMMDAWRS AMVLLNRDPRAEAFIQTYRDEQAVATPAAPVSDAPQEIRQRLGQAVINGDRDGIIGLVEEALAQGLAPLQISNEGFLPGL EEVGRRFEKNIFFLPQVMQSADTMHAGFARLKEEMKGQPLESRGRILMATVEGDIHDIGKNIVCTLLENHGFEVFDIGKN VSAATIVAKAREFGVDAVGLSALMTTTMAEMDTVLKKLKAVGIKAFTMIGGAVVTQEYADRIGADIYARDAMEAVAKVKK LLQIAD
Sequences:
>Translated_806_residues MTPFLEAIQERVLILDGAMGTMLQERGLRPGQSPEELNLTMPDVVASVHREYIEAGADIIITNSFGGSRFKLAHFGLEGR LAEINARAVEIARGEARGRAYVGGSMGPTGQFVEPLGEVSFDRMKESFREQAEALVSAGVDLISLETFLDIKECRAAVIA IREVSPTIPIIAMMTFDDNGRSVLGTPPEAAAITLAAAGVDIVGSNCGLGVEGIHDILVRMRRVTSLPLISQANAGLPIL KDGITVFPGTPREMTDYHDRLISLGVRVIGGCCGTTPAHIRAMKEALAGRQQVWQEPTHLEGMTLLSCRGGWTAVGPGQK TAIIGERINPTGKKLYSKELQEGKVSYIRREAMEQAQLGATLLDLNVGAPGIDEPVAMERAVFCASGAVGVPLVLDSSSP AALEAGLKAADGKVLINSVSGEAKSLSRVLPLAKKYGAALIGLTLDAKGIPDSAEGRLAIARRIRNAARRQGIPDQDIII DCLTLTVSAEQKRAAETLRTIRLVKEKLGLSTVLGVSNISFGLPQRPLISSSFFSMAMAAGLDAAIINPREKAMMDAWRS AMVLLNRDPRAEAFIQTYRDEQAVATPAAPVSDAPQEIRQRLGQAVINGDRDGIIGLVEEALAQGLAPLQISNEGFLPGL EEVGRRFEKNIFFLPQVMQSADTMHAGFARLKEEMKGQPLESRGRILMATVEGDIHDIGKNIVCTLLENHGFEVFDIGKN VSAATIVAKAREFGVDAVGLSALMTTTMAEMDTVLKKLKAVGIKAFTMIGGAVVTQEYADRIGADIYARDAMEAVAKVKK LLQIAD >Mature_805_residues TPFLEAIQERVLILDGAMGTMLQERGLRPGQSPEELNLTMPDVVASVHREYIEAGADIIITNSFGGSRFKLAHFGLEGRL AEINARAVEIARGEARGRAYVGGSMGPTGQFVEPLGEVSFDRMKESFREQAEALVSAGVDLISLETFLDIKECRAAVIAI REVSPTIPIIAMMTFDDNGRSVLGTPPEAAAITLAAAGVDIVGSNCGLGVEGIHDILVRMRRVTSLPLISQANAGLPILK DGITVFPGTPREMTDYHDRLISLGVRVIGGCCGTTPAHIRAMKEALAGRQQVWQEPTHLEGMTLLSCRGGWTAVGPGQKT AIIGERINPTGKKLYSKELQEGKVSYIRREAMEQAQLGATLLDLNVGAPGIDEPVAMERAVFCASGAVGVPLVLDSSSPA ALEAGLKAADGKVLINSVSGEAKSLSRVLPLAKKYGAALIGLTLDAKGIPDSAEGRLAIARRIRNAARRQGIPDQDIIID CLTLTVSAEQKRAAETLRTIRLVKEKLGLSTVLGVSNISFGLPQRPLISSSFFSMAMAAGLDAAIINPREKAMMDAWRSA MVLLNRDPRAEAFIQTYRDEQAVATPAAPVSDAPQEIRQRLGQAVINGDRDGIIGLVEEALAQGLAPLQISNEGFLPGLE EVGRRFEKNIFFLPQVMQSADTMHAGFARLKEEMKGQPLESRGRILMATVEGDIHDIGKNIVCTLLENHGFEVFDIGKNV SAATIVAKAREFGVDAVGLSALMTTTMAEMDTVLKKLKAVGIKAFTMIGGAVVTQEYADRIGADIYARDAMEAVAKVKKL LQIAD
Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]
COG id: COG1410
COG function: function code E; Methionine synthase I, cobalamin-binding domain
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pterin-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=879, Percent_Identity=30.3754266211604, Blast_Score=330, Evalue=2e-90, Organism=Homo sapiens, GI157266337, Length=303, Percent_Identity=30.03300330033, Blast_Score=105, Evalue=2e-22, Organism=Homo sapiens, GI13162290, Length=296, Percent_Identity=30.4054054054054, Blast_Score=102, Evalue=1e-21, Organism=Escherichia coli, GI1790450, Length=870, Percent_Identity=30.4597701149425, Blast_Score=325, Evalue=7e-90, Organism=Escherichia coli, GI1786456, Length=297, Percent_Identity=28.956228956229, Blast_Score=97, Evalue=4e-21, Organism=Caenorhabditis elegans, GI17535405, Length=852, Percent_Identity=30.9859154929577, Blast_Score=315, Evalue=7e-86, Organism=Drosophila melanogaster, GI24585077, Length=285, Percent_Identity=25.6140350877193, Blast_Score=67, Evalue=6e-11,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006158 - InterPro: IPR011005 - InterPro: IPR003759 - InterPro: IPR011822 - InterPro: IPR000489 - InterPro: IPR003726 - InterPro: IPR004223 [H]
Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]
EC number: =2.1.1.13 [H]
Molecular weight: Translated: 86170; Mature: 86039
Theoretical pI: Translated: 5.82; Mature: 5.82
Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.0 %Cys (Translated Protein) 3.5 %Met (Translated Protein) 4.5 %Cys+Met (Translated Protein) 1.0 %Cys (Mature Protein) 3.4 %Met (Mature Protein) 4.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTPFLEAIQERVLILDGAMGTMLQERGLRPGQSPEELNLTMPDVVASVHREYIEAGADII CCHHHHHHHHHHEEECCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE ITNSFGGSRFKLAHFGLEGRLAEINARAVEIARGEARGRAYVGGSMGPTGQFVEPLGEVS EEECCCCCEEEEEEECCCCEEECCCHHHEEEECCCCCCCEEECCCCCCCCHHHHHHHHHH FDRMKESFREQAEALVSAGVDLISLETFLDIKECRAAVIAIREVSPTIPIIAMMTFDDNG HHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC RSVLGTPPEAAAITLAAAGVDIVGSNCGLGVEGIHDILVRMRRVTSLPLISQANAGLPIL CEECCCCCCHHEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE KDGITVFPGTPREMTDYHDRLISLGVRVIGGCCGTTPAHIRAMKEALAGRQQVWQEPTHL ECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC EGMTLLSCRGGWTAVGPGQKTAIIGERINPTGKKLYSKELQEGKVSYIRREAMEQAQLGA CCEEEEEECCCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC TLLDLNVGAPGIDEPVAMERAVFCASGAVGVPLVLDSSSPAALEAGLKAADGKVLINSVS EEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHCCCCCCCEEEEEECC GEAKSLSRVLPLAKKYGAALIGLTLDAKGIPDSAEGRLAIARRIRNAARRQGIPDQDIII CHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHE DCLTLTVSAEQKRAAETLRTIRLVKEKLGLSTVLGVSNISFGLPQRPLISSSFFSMAMAA EHHHEEECHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH GLDAAIINPREKAMMDAWRSAMVLLNRDPRAEAFIQTYRDEQAVATPAAPVSDAPQEIRQ CCCCEEECCHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH RLGQAVINGDRDGIIGLVEEALAQGLAPLQISNEGFLPGLEEVGRRFEKNIFFLPQVMQS HHHHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCEEEHHHHHHH ADTMHAGFARLKEEMKGQPLESRGRILMATVEGDIHDIGKNIVCTLLENHGFEVFDIGKN HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHCHHHHHHHHHCCCCEEEECCCC VSAATIVAKAREFGVDAVGLSALMTTTMAEMDTVLKKLKAVGIKAFTMIGGAVVTQEYAD CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH RIGADIYARDAMEAVAKVKKLLQIAD HHCCCHHHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure TPFLEAIQERVLILDGAMGTMLQERGLRPGQSPEELNLTMPDVVASVHREYIEAGADII CHHHHHHHHHHEEECCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE ITNSFGGSRFKLAHFGLEGRLAEINARAVEIARGEARGRAYVGGSMGPTGQFVEPLGEVS EEECCCCCEEEEEEECCCCEEECCCHHHEEEECCCCCCCEEECCCCCCCCHHHHHHHHHH FDRMKESFREQAEALVSAGVDLISLETFLDIKECRAAVIAIREVSPTIPIIAMMTFDDNG HHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC RSVLGTPPEAAAITLAAAGVDIVGSNCGLGVEGIHDILVRMRRVTSLPLISQANAGLPIL CEECCCCCCHHEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE KDGITVFPGTPREMTDYHDRLISLGVRVIGGCCGTTPAHIRAMKEALAGRQQVWQEPTHL ECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC EGMTLLSCRGGWTAVGPGQKTAIIGERINPTGKKLYSKELQEGKVSYIRREAMEQAQLGA CCEEEEEECCCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC TLLDLNVGAPGIDEPVAMERAVFCASGAVGVPLVLDSSSPAALEAGLKAADGKVLINSVS EEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHCCCCCCCEEEEEECC GEAKSLSRVLPLAKKYGAALIGLTLDAKGIPDSAEGRLAIARRIRNAARRQGIPDQDIII CHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHE DCLTLTVSAEQKRAAETLRTIRLVKEKLGLSTVLGVSNISFGLPQRPLISSSFFSMAMAA EHHHEEECHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH GLDAAIINPREKAMMDAWRSAMVLLNRDPRAEAFIQTYRDEQAVATPAAPVSDAPQEIRQ CCCCEEECCHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH RLGQAVINGDRDGIIGLVEEALAQGLAPLQISNEGFLPGLEEVGRRFEKNIFFLPQVMQS HHHHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCEEEHHHHHHH ADTMHAGFARLKEEMKGQPLESRGRILMATVEGDIHDIGKNIVCTLLENHGFEVFDIGKN HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHCHHHHHHHHHCCCCEEEECCCC VSAATIVAKAREFGVDAVGLSALMTTTMAEMDTVLKKLKAVGIKAFTMIGGAVVTQEYAD CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH RIGADIYARDAMEAVAKVKKLLQIAD HHCCCHHHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9634230 [H]