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The map label for this gene is fixL [H]

Identifier: 118579820

GI number: 118579820

Start: 1488668

End: 1490893

Strand: Reverse

Name: fixL [H]

Synonym: Ppro_1396

Alternate gene names: 118579820

Gene position: 1490893-1488668 (Counterclockwise)

Preceding gene: 118579825

Following gene: 118579816

Centisome position: 37.2

GC content: 57.1

Gene sequence:

>2226_bases
GTGGCCAACTCCCCCCCCTTTTCCCGACTCGTCAATATCAATCAACTACAGCAGCTGCTGGAAGCCCAGTACAGAATCAA
CGGCACACCATCCGCCATCATGGATGAGGATGAAAACATCCTGGTGGCAGTCGGCTGGCAGGATCTCTGCGTCCGCTTCC
ACCGCGTACATCCGGACAGCTGTGTCCGTTGCCGTGAGAGTGACAGGTACATCAAGTCCCATCTGCATTCTTTTGACGGA
GAATATCTGGACTACACGTGCAAGAACGGCCTGCGGGATGTCGCCATGCCGATCAGGATCAACGGAGAGCATGTGGCCAC
ATTCTTTACCGGGCAGTTTTTCTACGACGACGACAAGCCGGACCCGGACTATTTTCGTGCCCAGGCCAGGGAATTCGGCT
TTAACGAAAACGACTATATCCGGGCCATGGGCATGATTCCCCTACGCAGCCGCGAACAGATCAGAAACATAATGGAATAT
TCACGCAATCTGGTTCAGGTAATGGCCGAACAGGGCCTGAACAACCTGAAACTGGCCCGGGAGGTGGAGGAACGGAAAAA
AGTCGAGCAGGAAGCCTCTTTTTTCAAGACCCTGATCGAGTACACGCGCGACCCGGTCTATGTGCTTGATCCGGCGGACG
GATTCCGCATGGCCTATGCCAACCAGGCGGCCTGTTCCCACTTCGGGAAAACCCTCGAAGAGCTGCTCACCATGCGCATT
CCCGACTGGGACCCCGTATTCGACATGAAAAACGTCGATGTCAGCCTGGAGCAGATGAAGCAGGGCAGGTCGCTGCTGTT
CGAGACAACCCACCGAAGCGCCTCGGGACGGCTGATTCCCGTGGAAGTGACCAGTAGCTACCTGGAGCACAACGGACGGG
GACTGACCATGGGGCACTTCCACGACATCACCGAGCGCAAGAAGGCTGAAGAGCAGATACTTCGCTTGAACCGGCTCTAC
GCGGTGCTGAGCGAAACCAACAAGGCCATTGTCCACTCCAGCGACCGCCAGATCCTGTTCCAGCGAATCTGTCGGGTTAT
CGTTGAGCATGGCGGCTTCCAGTTGGCCTGGATAGGAGTGGTGGACCAGGATACCGCCCTGGTGAAATCCGCCGCATGGA
GCAGCAAAGACGATTCCTGCCTGGAAGAGTTCAGGGTTTTGCTCAGGGAAGAGTCGCAGGCAATGGGGGGCATCGCTTCC
GCCATCCGCGGTGGAAAGCTGGAAATAGTCAACGACTTCCTGGATGATCCGGGCAGCGGGCCTTGGCACAGGGAGGCGGA
AAGCCACGGGTACCGGTCGGTGGCGGCCATCGCCCTGAAATCGAACGAAAAGGCCGTCGGTGTCCTGACCATCTACGCCG
CTGAAACGTTGTTTTTCCATGGGGGCATGGACGATCTGCTGCTGCAGTTGGCAAGCGACATCTCCTTCGCCCTGGACAAC
CTGGACCGGGAGGCCCGCAGGAGGGAGACTGAGTGGGCCTTGCGGGAGGAGACCCTGCAACGCCTACGGACGGCGGAAGA
ACTCCGCGAAAAAGAACGCCTGCTTGTGCTGCAAAGCCGCCAGGCGGCGATGGGGGAGATGATCGAGAACATAGCCCACC
AGTGGCGTCAGCCGCTCAATACGCTGGGGCTTATCCTCCAGGAACTGCCCATGACCCATGGTGACGGCGAACTGAGCAGG
GAATACCTGGACGATGCGGTGGAGAGGGCCATGGAACAGATTCTGCATATGTCCCAGACCATAAATGATTTCAGCAATTT
TTTCAGACCGGACAAGCAGAAACTCACCTTCAGGGTGCAGCAGGTCGTCACCAGAACCCTGTCCCTGATCGCGGGAAACT
TCAGAAAAACGGCTATTTCCGTAGAAATAAACGCATTGGACGATCCTGTCATCACCGGTTATCCCAATGAGTATGCACAG
GTCCTGCTGACCGTATTTCTTAACGCACGGGATGTTTTTTCGGAACGGGAGACCCCCTACCCCAGGCTCACGGTAACCAT
TGGCGTCGAAAAGGGGAGGAGCGTGGTGACCATTGCCGACAACGGCGGCGGCATTTCCAGCGACATCATCGACAGGATTT
TTGAAGCGTACGTCACCACCAAGGGGCCGGACAGGGGAACCGGCGTGGGGCTGTTCATGTCCAAGGCAATCATCGAGAAG
AACATGCACGGCTCCCTTTTCGTGCGCAACACGAAACAGGGCGCCGAATTCAGGATCGAGGTCTGA

Upstream 100 bases:

>100_bases
CGATCATTCAGCAACGGTATATTTTGTGGCCCTTCATCCGATCCGCCCGCGGGCGTTATCGGATTCGGCGGCATATGAAC
ACAAAGGCATTGAGGTTAGC

Downstream 100 bases:

>100_bases
GTGTACAGGTTCTGAGCGACCATACCCATCGGCCGTTGAGCGGCGCTGGCCTCTCGACGTAGAGCTTAGGCTCCGCGGGA
GTTAAAAACGGCTCGTTGAA

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 741; Mature: 740

Protein sequence:

>741_residues
MANSPPFSRLVNINQLQQLLEAQYRINGTPSAIMDEDENILVAVGWQDLCVRFHRVHPDSCVRCRESDRYIKSHLHSFDG
EYLDYTCKNGLRDVAMPIRINGEHVATFFTGQFFYDDDKPDPDYFRAQAREFGFNENDYIRAMGMIPLRSREQIRNIMEY
SRNLVQVMAEQGLNNLKLAREVEERKKVEQEASFFKTLIEYTRDPVYVLDPADGFRMAYANQAACSHFGKTLEELLTMRI
PDWDPVFDMKNVDVSLEQMKQGRSLLFETTHRSASGRLIPVEVTSSYLEHNGRGLTMGHFHDITERKKAEEQILRLNRLY
AVLSETNKAIVHSSDRQILFQRICRVIVEHGGFQLAWIGVVDQDTALVKSAAWSSKDDSCLEEFRVLLREESQAMGGIAS
AIRGGKLEIVNDFLDDPGSGPWHREAESHGYRSVAAIALKSNEKAVGVLTIYAAETLFFHGGMDDLLLQLASDISFALDN
LDREARRRETEWALREETLQRLRTAEELREKERLLVLQSRQAAMGEMIENIAHQWRQPLNTLGLILQELPMTHGDGELSR
EYLDDAVERAMEQILHMSQTINDFSNFFRPDKQKLTFRVQQVVTRTLSLIAGNFRKTAISVEINALDDPVITGYPNEYAQ
VLLTVFLNARDVFSERETPYPRLTVTIGVEKGRSVVTIADNGGGISSDIIDRIFEAYVTTKGPDRGTGVGLFMSKAIIEK
NMHGSLFVRNTKQGAEFRIEV

Sequences:

>Translated_741_residues
MANSPPFSRLVNINQLQQLLEAQYRINGTPSAIMDEDENILVAVGWQDLCVRFHRVHPDSCVRCRESDRYIKSHLHSFDG
EYLDYTCKNGLRDVAMPIRINGEHVATFFTGQFFYDDDKPDPDYFRAQAREFGFNENDYIRAMGMIPLRSREQIRNIMEY
SRNLVQVMAEQGLNNLKLAREVEERKKVEQEASFFKTLIEYTRDPVYVLDPADGFRMAYANQAACSHFGKTLEELLTMRI
PDWDPVFDMKNVDVSLEQMKQGRSLLFETTHRSASGRLIPVEVTSSYLEHNGRGLTMGHFHDITERKKAEEQILRLNRLY
AVLSETNKAIVHSSDRQILFQRICRVIVEHGGFQLAWIGVVDQDTALVKSAAWSSKDDSCLEEFRVLLREESQAMGGIAS
AIRGGKLEIVNDFLDDPGSGPWHREAESHGYRSVAAIALKSNEKAVGVLTIYAAETLFFHGGMDDLLLQLASDISFALDN
LDREARRRETEWALREETLQRLRTAEELREKERLLVLQSRQAAMGEMIENIAHQWRQPLNTLGLILQELPMTHGDGELSR
EYLDDAVERAMEQILHMSQTINDFSNFFRPDKQKLTFRVQQVVTRTLSLIAGNFRKTAISVEINALDDPVITGYPNEYAQ
VLLTVFLNARDVFSERETPYPRLTVTIGVEKGRSVVTIADNGGGISSDIIDRIFEAYVTTKGPDRGTGVGLFMSKAIIEK
NMHGSLFVRNTKQGAEFRIEV
>Mature_740_residues
ANSPPFSRLVNINQLQQLLEAQYRINGTPSAIMDEDENILVAVGWQDLCVRFHRVHPDSCVRCRESDRYIKSHLHSFDGE
YLDYTCKNGLRDVAMPIRINGEHVATFFTGQFFYDDDKPDPDYFRAQAREFGFNENDYIRAMGMIPLRSREQIRNIMEYS
RNLVQVMAEQGLNNLKLAREVEERKKVEQEASFFKTLIEYTRDPVYVLDPADGFRMAYANQAACSHFGKTLEELLTMRIP
DWDPVFDMKNVDVSLEQMKQGRSLLFETTHRSASGRLIPVEVTSSYLEHNGRGLTMGHFHDITERKKAEEQILRLNRLYA
VLSETNKAIVHSSDRQILFQRICRVIVEHGGFQLAWIGVVDQDTALVKSAAWSSKDDSCLEEFRVLLREESQAMGGIASA
IRGGKLEIVNDFLDDPGSGPWHREAESHGYRSVAAIALKSNEKAVGVLTIYAAETLFFHGGMDDLLLQLASDISFALDNL
DREARRRETEWALREETLQRLRTAEELREKERLLVLQSRQAAMGEMIENIAHQWRQPLNTLGLILQELPMTHGDGELSRE
YLDDAVERAMEQILHMSQTINDFSNFFRPDKQKLTFRVQQVVTRTLSLIAGNFRKTAISVEINALDDPVITGYPNEYAQV
LLTVFLNARDVFSERETPYPRLTVTIGVEKGRSVVTIADNGGGISSDIIDRIFEAYVTTKGPDRGTGVGLFMSKAIIEKN
MHGSLFVRNTKQGAEFRIEV

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=235, Percent_Identity=25.1063829787234, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 84483; Mature: 84352

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSPPFSRLVNINQLQQLLEAQYRINGTPSAIMDEDENILVAVGWQDLCVRFHRVHPDS
CCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCHHCCCCCCEEEEECHHHHHHHHHHCCCHH
CVRCRESDRYIKSHLHSFDGEYLDYTCKNGLRDVAMPIRINGEHVATFFTGQFFYDDDKP
HHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCHHCEEEEEECCCEEEEEEEEEEEECCCCC
DPDYFRAQAREFGFNENDYIRAMGMIPLRSREQIRNIMEYSRNLVQVMAEQGLNNLKLAR
CHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
EVEERKKVEQEASFFKTLIEYTRDPVYVLDPADGFRMAYANQAACSHFGKTLEELLTMRI
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEHHCCHHHHHHHHHHHHHHHHHCC
PDWDPVFDMKNVDVSLEQMKQGRSLLFETTHRSASGRLIPVEVTSSYLEHNGRGLTMGHF
CCCCCCCCCCCCCCCHHHHHCCHHHEEHHHCCCCCCCEEEEHHHHHHHHCCCCEEEEHHH
HDITERKKAEEQILRLNRLYAVLSETNKAIVHSSDRQILFQRICRVIVEHGGFQLAWIGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHCCCEEEEEEEE
VDQDTALVKSAAWSSKDDSCLEEFRVLLREESQAMGGIASAIRGGKLEIVNDFLDDPGSG
ECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCC
PWHREAESHGYRSVAAIALKSNEKAVGVLTIYAAETLFFHGGMDDLLLQLASDISFALDN
CCCCCHHCCCCHHEEEEEEECCCCEEEEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHH
LDREARRRETEWALREETLQRLRTAEELREKERLLVLQSRQAAMGEMIENIAHQWRQPLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHH
TLGLILQELPMTHGDGELSREYLDDAVERAMEQILHMSQTINDFSNFFRPDKQKLTFRVQ
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QVVTRTLSLIAGNFRKTAISVEINALDDPVITGYPNEYAQVLLTVFLNARDVFSERETPY
HHHHHHHHHHHCCCEEEEEEEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCC
PRLTVTIGVEKGRSVVTIADNGGGISSDIIDRIFEAYVTTKGPDRGTGVGLFMSKAIIEK
CEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
NMHGSLFVRNTKQGAEFRIEV
CCCCCEEEEECCCCCCEEEEC
>Mature Secondary Structure 
ANSPPFSRLVNINQLQQLLEAQYRINGTPSAIMDEDENILVAVGWQDLCVRFHRVHPDS
CCCCCHHHHCCHHHHHHHHHHHHHCCCCCCHHCCCCCCEEEEECHHHHHHHHHHCCCHH
CVRCRESDRYIKSHLHSFDGEYLDYTCKNGLRDVAMPIRINGEHVATFFTGQFFYDDDKP
HHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCHHCEEEEEECCCEEEEEEEEEEEECCCCC
DPDYFRAQAREFGFNENDYIRAMGMIPLRSREQIRNIMEYSRNLVQVMAEQGLNNLKLAR
CHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
EVEERKKVEQEASFFKTLIEYTRDPVYVLDPADGFRMAYANQAACSHFGKTLEELLTMRI
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEHHCCHHHHHHHHHHHHHHHHHCC
PDWDPVFDMKNVDVSLEQMKQGRSLLFETTHRSASGRLIPVEVTSSYLEHNGRGLTMGHF
CCCCCCCCCCCCCCCHHHHHCCHHHEEHHHCCCCCCCEEEEHHHHHHHHCCCCEEEEHHH
HDITERKKAEEQILRLNRLYAVLSETNKAIVHSSDRQILFQRICRVIVEHGGFQLAWIGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHCCCEEEEEEEE
VDQDTALVKSAAWSSKDDSCLEEFRVLLREESQAMGGIASAIRGGKLEIVNDFLDDPGSG
ECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCC
PWHREAESHGYRSVAAIALKSNEKAVGVLTIYAAETLFFHGGMDDLLLQLASDISFALDN
CCCCCHHCCCCHHEEEEEEECCCCEEEEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHH
LDREARRRETEWALREETLQRLRTAEELREKERLLVLQSRQAAMGEMIENIAHQWRQPLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHH
TLGLILQELPMTHGDGELSREYLDDAVERAMEQILHMSQTINDFSNFFRPDKQKLTFRVQ
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QVVTRTLSLIAGNFRKTAISVEINALDDPVITGYPNEYAQVLLTVFLNARDVFSERETPY
HHHHHHHHHHHCCCEEEEEEEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCC
PRLTVTIGVEKGRSVVTIADNGGGISSDIIDRIFEAYVTTKGPDRGTGVGLFMSKAIIEK
CEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
NMHGSLFVRNTKQGAEFRIEV
CCCCCEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]