Definition Pelobacter propionicus DSM 2379 chromosome, complete genome.
Accession NC_008609
Length 4,008,000

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The map label for this gene is purT

Identifier: 118579814

GI number: 118579814

Start: 1482408

End: 1483592

Strand: Direct

Name: purT

Synonym: Ppro_1390

Alternate gene names: 118579814

Gene position: 1482408-1483592 (Clockwise)

Preceding gene: 118579810

Following gene: 118579815

Centisome position: 36.99

GC content: 65.99

Gene sequence:

>1185_bases
ATGAATCTGGGAACACCATTGAAACCGGGCGCAACCAGGGTCCTGCTGCTGGGGTCCGGGGAACTGGGCAAGGAAGTCGC
CATCGAGGCCCAGCGCCTGGGGGTTGAGGTGATTGCCGTTGACCGCTATGCCGATGCTCCGGCCATGCAGGTGGCGCATC
GCAGCCATGTCATCAGCATGCAGGACCGTGAGGAGCTGAAAAGGGTGATCGGCCTGGAACGCCCCGACCTGATCGTGCCG
GAGATCGAGGCCATCGACACCGAATTCCTGCTGGAGCTTGAGCAGGGAGGTCAACGGGTGATCCCCACCGCCCGGGCCAC
CAACCTGACCATGAACCGGGAGGGGATCCGGCGGCTGGCAGCCGAAGAACTGGGTTTGCCCACGGCGCGCTACGCTTTTG
CCGCCAGCCTGGAGGAGATGCGCGGTCATGTGGCCCAGATCGGCTGCCCCTGCGTCATCAAGCCGATTATGAGCTCCTCG
GGCAAGGGGCAGAGCGTGCTGCGCGACCCCTCACAGGTGGACGAGGCCTGGCGCTATGCCATGGAGGGGGCGCGGGGCGC
CTCGGATACGGTGATCATCGAGGAGTTCATTCAGTTCGACTACGAGATCACCCTGCTGACAGTGCGCCACTGCAACGGCA
CTTCCTTCTGCCCTCCCATTGGCCATGTGCAGATCAAGGGGGACTACCACGAGTCCTGGCAGCCCATGGCCATGAGTCCC
GCGGCCCTGGCCGAGGCCAGGCGCCAGGCCGAGGCGGTCACCACCGCCCTGGGGGGCTACGGTCTTTTCGGCGTGGAGCT
GTTCATCAGCAACGACCGGGTCCTGTTCAGCGAGGTCTCGCCCCGTCCCCACGACACCGGCATGGTCACCATGATCTCCC
AGAACCTGTCCCAGTTTGAGCTGCACGTGCGCGCCATCCTGGGGCTGCCGGTGGGCGAGATCGTCAACCTGGCCCCCTCG
GCCAGTCATGTGATCCTGGCCGACGCCAGCTTTGATGCGGTGCGCTTCGAGGGGCTGGAGCAGGCCTTGGAGGTAGCCAC
CAGCAAGCTGCGCCTGTTCGGCAAACCGGATACCCGCCCCGGCCGGCGCATGGGGGTCGCCCTGGTGCAGGGGAAAGACA
CGGACGAGGCCCGCGGCCGCGCCGAAGCCTGCGCCCACGCCGTACGCATGGTTCCATTGTCGTAG

Upstream 100 bases:

>100_bases
TGGTCTGGGGCGTATTTGGGCTTGATTTTTCGGATGGTTGATGTAGTTCTAACAGCGATTGTTCGATTATTTTAACAGTA
CATGTGGAGGAAGCGAGATT

Downstream 100 bases:

>100_bases
CATTTGTAAAATACATTCCGTTGCACACGAAGCAGGCCGCATCCCCACCGGGTGCGGTCTGCTTCGTCTGAGTCGGGACT
TCGGTGCCTGGAGTGCTCCG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNLGTPLKPGATRVLLLGSGELGKEVAIEAQRLGVEVIAVDRYADAPAMQVAHRSHVISMQDREELKRVIGLERPDLIVP
EIEAIDTEFLLELEQGGQRVIPTARATNLTMNREGIRRLAAEELGLPTARYAFAASLEEMRGHVAQIGCPCVIKPIMSSS
GKGQSVLRDPSQVDEAWRYAMEGARGASDTVIIEEFIQFDYEITLLTVRHCNGTSFCPPIGHVQIKGDYHESWQPMAMSP
AALAEARRQAEAVTTALGGYGLFGVELFISNDRVLFSEVSPRPHDTGMVTMISQNLSQFELHVRAILGLPVGEIVNLAPS
ASHVILADASFDAVRFEGLEQALEVATSKLRLFGKPDTRPGRRMGVALVQGKDTDEARGRAEACAHAVRMVPLS

Sequences:

>Translated_394_residues
MNLGTPLKPGATRVLLLGSGELGKEVAIEAQRLGVEVIAVDRYADAPAMQVAHRSHVISMQDREELKRVIGLERPDLIVP
EIEAIDTEFLLELEQGGQRVIPTARATNLTMNREGIRRLAAEELGLPTARYAFAASLEEMRGHVAQIGCPCVIKPIMSSS
GKGQSVLRDPSQVDEAWRYAMEGARGASDTVIIEEFIQFDYEITLLTVRHCNGTSFCPPIGHVQIKGDYHESWQPMAMSP
AALAEARRQAEAVTTALGGYGLFGVELFISNDRVLFSEVSPRPHDTGMVTMISQNLSQFELHVRAILGLPVGEIVNLAPS
ASHVILADASFDAVRFEGLEQALEVATSKLRLFGKPDTRPGRRMGVALVQGKDTDEARGRAEACAHAVRMVPLS
>Mature_394_residues
MNLGTPLKPGATRVLLLGSGELGKEVAIEAQRLGVEVIAVDRYADAPAMQVAHRSHVISMQDREELKRVIGLERPDLIVP
EIEAIDTEFLLELEQGGQRVIPTARATNLTMNREGIRRLAAEELGLPTARYAFAASLEEMRGHVAQIGCPCVIKPIMSSS
GKGQSVLRDPSQVDEAWRYAMEGARGASDTVIIEEFIQFDYEITLLTVRHCNGTSFCPPIGHVQIKGDYHESWQPMAMSP
AALAEARRQAEAVTTALGGYGLFGVELFISNDRVLFSEVSPRPHDTGMVTMISQNLSQFELHVRAILGLPVGEIVNLAPS
ASHVILADASFDAVRFEGLEQALEVATSKLRLFGKPDTRPGRRMGVALVQGKDTDEARGRAEACAHAVRMVPLS

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=388, Percent_Identity=60.5670103092783, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786733, Length=374, Percent_Identity=26.2032085561497, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324702, Length=365, Percent_Identity=25.4794520547945, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_PELPD (A1ANT6)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_901064.1
- ProteinModelPortal:   A1ANT6
- SMR:   A1ANT6
- STRING:   A1ANT6
- GeneID:   4573606
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_1390
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- PhylomeDB:   A1ANT6
- ProtClustDB:   PRK09288
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42867; Mature: 42867

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 81-81 BINDING 113-113 BINDING 154-154 BINDING 202-202 BINDING 266-266 BINDING 278-278 BINDING 285-285 BINDING 355-355

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MNLGTPLKPGATRVLLLGSGELGKEVAIEAQRLGVEVIAVDRYADAPAMQVAHRSHVISM
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHEECC
QDREELKRVIGLERPDLIVPEIEAIDTEFLLELEQGGQRVIPTARATNLTMNREGIRRLA
CCHHHHHHHHCCCCCCEECCCHHHCCHHHHHHHHCCCCEECCCCCCCEEEECHHHHHHHH
AEELGLPTARYAFAASLEEMRGHVAQIGCPCVIKPIMSSSGKGQSVLRDPSQVDEAWRYA
HHHHCCCHHHHHHHHHHHHHCCHHHHCCCCCEEHHHHCCCCCCCHHHCCHHHHHHHHHHH
MEGARGASDTVIIEEFIQFDYEITLLTVRHCNGTSFCPPIGHVQIKGDYHESWQPMAMSP
HHCCCCCCCHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCH
AALAEARRQAEAVTTALGGYGLFGVELFISNDRVLFSEVSPRPHDTGMVTMISQNLSQFE
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCEEEEECCCHHHHH
LHVRAILGLPVGEIVNLAPSASHVILADASFDAVRFEGLEQALEVATSKLRLFGKPDTRP
HHHHHHHCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCCCC
GRRMGVALVQGKDTDEARGRAEACAHAVRMVPLS
CHHEEEEEEECCCCHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNLGTPLKPGATRVLLLGSGELGKEVAIEAQRLGVEVIAVDRYADAPAMQVAHRSHVISM
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHEECC
QDREELKRVIGLERPDLIVPEIEAIDTEFLLELEQGGQRVIPTARATNLTMNREGIRRLA
CCHHHHHHHHCCCCCCEECCCHHHCCHHHHHHHHCCCCEECCCCCCCEEEECHHHHHHHH
AEELGLPTARYAFAASLEEMRGHVAQIGCPCVIKPIMSSSGKGQSVLRDPSQVDEAWRYA
HHHHCCCHHHHHHHHHHHHHCCHHHHCCCCCEEHHHHCCCCCCCHHHCCHHHHHHHHHHH
MEGARGASDTVIIEEFIQFDYEITLLTVRHCNGTSFCPPIGHVQIKGDYHESWQPMAMSP
HHCCCCCCCHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCH
AALAEARRQAEAVTTALGGYGLFGVELFISNDRVLFSEVSPRPHDTGMVTMISQNLSQFE
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCEEEEECCCHHHHH
LHVRAILGLPVGEIVNLAPSASHVILADASFDAVRFEGLEQALEVATSKLRLFGKPDTRP
HHHHHHHCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCCCC
GRRMGVALVQGKDTDEARGRAEACAHAVRMVPLS
CHHEEEEEEECCCCHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA