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The map label for this gene is pleD [H]

Identifier: 118579804

GI number: 118579804

Start: 1468303

End: 1469667

Strand: Direct

Name: pleD [H]

Synonym: Ppro_1380

Alternate gene names: 118579804

Gene position: 1468303-1469667 (Clockwise)

Preceding gene: 118579803

Following gene: 118579805

Centisome position: 36.63

GC content: 60.29

Gene sequence:

>1365_bases
ATGCGCAGAATACTGGTAGTCGAAGATGACCGGTTCTTCCGTGACCTGTTTTACGACCTGCTGGTCGGCCAGGGATACGA
TGTGGATCGTGCCTCAAGCGGGGAGGAGGGGCTCGACAGGCTATCGACGTATGCCTTCGACCTGGTCGTCACCGATCTGG
TCATGCCCGGTGTGGACGGCATGGACATCCTTGCCAGGGCCCGGGAGAACGATCCTTCCGCGGACGTGATCATGGTTACC
GGCAATGCCAACCTGGAATCGGCCATCTTCGCCCTCAAGCATGGCGCCCGCGATTATTTCGTCAAACCGATCAACCCCGA
CGAGTTCCTCCACTCCGTGGCCCAGTGCCTGGAACAACGCCGCATTCTGGACGAGAACGAAGAGCTGAAAAGCATGCTCA
ACCTGTACCAGATCAGCCAGGCCATTGCCGGCTGCCTGGACATGGAGCGTCTTCAGCACCTTATCTTTGATGCCTTCACC
CGTGAAATCGGTACCAGCCGCGGCATGTGCCTCTTTGCCACGGAAACTGGCCTGGAGCTGTGCGAGGTCAAGGGAGTCGA
GACCGCCGTGGCGGAGCGCTGCATCGCCTCTGTCCTGGAACGACTGTCAGAGGATCACCCTGATGAGTGCAACTCCCTGC
GGATAAGCTTCCAGGGCGGTGGCGATGATTCGGGGATCGAGGCGGCAATCCTGATTCCGCTGCGCGGCAAAGGGAGCCAG
CGGGGGGTTGTGGTGGCGTTCAACGAGCCGGGGCTGGGCCTTCCCGAATTAGGCGCCAGGAAGAAGAATATCCTCTTCCT
GCTGGAACAGTCGCTGCTGGCGCTGGAGAATGCCAGCAGCTATTCCCTGGCAAAGGACATGCTGTTCATCGACGACCTGA
GCGGGCTCTACAACCAGCGCTATCTGGAGGTGGCGCTGGAACGGGAGATGAAGCGCATCGGGCGCTTTTCCTCCCAGCTG
GCCGTTCTCTTCCTGGATATGGATTCGTTCAAGCAGGTCAACGACACCCATGGGCATCTGGTGGGGAGCCGCGTGCTCAA
GGAGATGGGCACGCTTTTGAGGCTGTCGGTGCGCGATGTGGACGTGGTCATCCGCTATGGCGGGGACGAGTACACCGCCA
TCCTGGTGGAGACATCGCCGGCCATTGCCGCCAATGTCGCCGAGCGTATACGATCCATGGTCGCGTCCCACGTCTTCCTG
GCTGACGAGGGGTACGACATCCGTCTGACCTGCAGCATCGGCTATTCCTGCTGCCCCGAAGACGCCCTGACCAAGGAAGA
ACTGCTGGAGATGGCCGACCAAGCCATGTACACCGGCAAGGGGCGCGGAAAAAACTGCGTTGTCCGCTTCACCAAGACCT
CCTGA

Upstream 100 bases:

>100_bases
AATTGATCCGTGCGGCTTAATTGCCGTTTGACAGTGGTTTCATAACGCGGTACTAATACATTCTTGACTGATTTCAAACC
CATGGGCAGAGATTGATCGC

Downstream 100 bases:

>100_bases
TTTTCCCGTTTTTCCCCCACATCACATCAGATCGAGGAATTCTATGGCCATCACGGCAATCAAGGGTTGTAACGATATCC
TGCCGGAGGAGTCCGGCAGA

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MRRILVVEDDRFFRDLFYDLLVGQGYDVDRASSGEEGLDRLSTYAFDLVVTDLVMPGVDGMDILARARENDPSADVIMVT
GNANLESAIFALKHGARDYFVKPINPDEFLHSVAQCLEQRRILDENEELKSMLNLYQISQAIAGCLDMERLQHLIFDAFT
REIGTSRGMCLFATETGLELCEVKGVETAVAERCIASVLERLSEDHPDECNSLRISFQGGGDDSGIEAAILIPLRGKGSQ
RGVVVAFNEPGLGLPELGARKKNILFLLEQSLLALENASSYSLAKDMLFIDDLSGLYNQRYLEVALEREMKRIGRFSSQL
AVLFLDMDSFKQVNDTHGHLVGSRVLKEMGTLLRLSVRDVDVVIRYGGDEYTAILVETSPAIAANVAERIRSMVASHVFL
ADEGYDIRLTCSIGYSCCPEDALTKEELLEMADQAMYTGKGRGKNCVVRFTKTS

Sequences:

>Translated_454_residues
MRRILVVEDDRFFRDLFYDLLVGQGYDVDRASSGEEGLDRLSTYAFDLVVTDLVMPGVDGMDILARARENDPSADVIMVT
GNANLESAIFALKHGARDYFVKPINPDEFLHSVAQCLEQRRILDENEELKSMLNLYQISQAIAGCLDMERLQHLIFDAFT
REIGTSRGMCLFATETGLELCEVKGVETAVAERCIASVLERLSEDHPDECNSLRISFQGGGDDSGIEAAILIPLRGKGSQ
RGVVVAFNEPGLGLPELGARKKNILFLLEQSLLALENASSYSLAKDMLFIDDLSGLYNQRYLEVALEREMKRIGRFSSQL
AVLFLDMDSFKQVNDTHGHLVGSRVLKEMGTLLRLSVRDVDVVIRYGGDEYTAILVETSPAIAANVAERIRSMVASHVFL
ADEGYDIRLTCSIGYSCCPEDALTKEELLEMADQAMYTGKGRGKNCVVRFTKTS
>Mature_454_residues
MRRILVVEDDRFFRDLFYDLLVGQGYDVDRASSGEEGLDRLSTYAFDLVVTDLVMPGVDGMDILARARENDPSADVIMVT
GNANLESAIFALKHGARDYFVKPINPDEFLHSVAQCLEQRRILDENEELKSMLNLYQISQAIAGCLDMERLQHLIFDAFT
REIGTSRGMCLFATETGLELCEVKGVETAVAERCIASVLERLSEDHPDECNSLRISFQGGGDDSGIEAAILIPLRGKGSQ
RGVVVAFNEPGLGLPELGARKKNILFLLEQSLLALENASSYSLAKDMLFIDDLSGLYNQRYLEVALEREMKRIGRFSSQL
AVLFLDMDSFKQVNDTHGHLVGSRVLKEMGTLLRLSVRDVDVVIRYGGDEYTAILVETSPAIAANVAERIRSMVASHVFL
ADEGYDIRLTCSIGYSCCPEDALTKEELLEMADQAMYTGKGRGKNCVVRFTKTS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=209, Percent_Identity=33.9712918660287, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=35.6687898089172, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786584, Length=212, Percent_Identity=27.8301886792453, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=32.5581395348837, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081974, Length=100, Percent_Identity=39, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=35, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787541, Length=161, Percent_Identity=29.8136645962733, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1790437, Length=121, Percent_Identity=34.7107438016529, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788381, Length=159, Percent_Identity=32.7044025157233, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=39.4957983193277, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87081881, Length=159, Percent_Identity=32.0754716981132, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790299, Length=105, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788550, Length=134, Percent_Identity=30.5970149253731, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790863, Length=106, Percent_Identity=36.7924528301887, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788905, Length=116, Percent_Identity=33.6206896551724, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1789402, Length=101, Percent_Identity=36.6336633663366, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788956, Length=160, Percent_Identity=32.5, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788085, Length=160, Percent_Identity=28.75, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788713, Length=127, Percent_Identity=33.0708661417323, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI87082012, Length=141, Percent_Identity=29.7872340425532, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787802, Length=160, Percent_Identity=30.625, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1787375, Length=108, Percent_Identity=30.5555555555556, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50391; Mature: 50391

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRILVVEDDRFFRDLFYDLLVGQGYDVDRASSGEEGLDRLSTYAFDLVVTDLVMPGVDG
CCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
MDILARARENDPSADVIMVTGNANLESAIFALKHGARDYFVKPINPDEFLHSVAQCLEQR
HHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHH
RILDENEELKSMLNLYQISQAIAGCLDMERLQHLIFDAFTREIGTSRGMCLFATETGLEL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHH
CEVKGVETAVAERCIASVLERLSEDHPDECNSLRISFQGGGDDSGIEAAILIPLRGKGSQ
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCC
RGVVVAFNEPGLGLPELGARKKNILFLLEQSLLALENASSYSLAKDMLFIDDLSGLYNQR
CCEEEEECCCCCCCCCCCCCCCHHHEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
YLEVALEREMKRIGRFSSQLAVLFLDMDSFKQVNDTHGHLVGSRVLKEMGTLLRLSVRDV
HHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECCE
DVVIRYGGDEYTAILVETSPAIAANVAERIRSMVASHVFLADEGYDIRLTCSIGYSCCPE
EEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEECCCCCCCC
DALTKEELLEMADQAMYTGKGRGKNCVVRFTKTS
CCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MRRILVVEDDRFFRDLFYDLLVGQGYDVDRASSGEEGLDRLSTYAFDLVVTDLVMPGVDG
CCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
MDILARARENDPSADVIMVTGNANLESAIFALKHGARDYFVKPINPDEFLHSVAQCLEQR
HHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHH
RILDENEELKSMLNLYQISQAIAGCLDMERLQHLIFDAFTREIGTSRGMCLFATETGLEL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHH
CEVKGVETAVAERCIASVLERLSEDHPDECNSLRISFQGGGDDSGIEAAILIPLRGKGSQ
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCC
RGVVVAFNEPGLGLPELGARKKNILFLLEQSLLALENASSYSLAKDMLFIDDLSGLYNQR
CCEEEEECCCCCCCCCCCCCCCHHHEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
YLEVALEREMKRIGRFSSQLAVLFLDMDSFKQVNDTHGHLVGSRVLKEMGTLLRLSVRDV
HHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECCE
DVVIRYGGDEYTAILVETSPAIAANVAERIRSMVASHVFLADEGYDIRLTCSIGYSCCPE
EEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEECCCCCCCC
DALTKEELLEMADQAMYTGKGRGKNCVVRFTKTS
CCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA