Click here to switch to the map view.

The map label for this gene is btuB [H]

Identifier: 118579689

GI number: 118579689

Start: 1322278

End: 1324023

Strand: Direct

Name: btuB [H]

Synonym: Ppro_1260

Alternate gene names: 118579689

Gene position: 1322278-1324023 (Clockwise)

Preceding gene: 118579688

Following gene: 118579690

Centisome position: 32.99

GC content: 62.2

Gene sequence:

>1746_bases
GTGCCCTTTCCTGATCGGCATGGCGTTGGTGGGCGCGCTTTTTTCCTCTGTCCGCTGATCGCGGTTGTGTCGGTCCTCGG
GCCCGTGACCGCGTCGGCCCAGGAGCGGGACGACATGATCGTGATCACGGCCGATGAGATCGCGGCCATGAAAGCCAACA
GGATGGAGGAGCTGCTCAACCACGTGCCGGGGGTTTCGGCCGGAGACTCGTCCATCAGCATCCACGGCTCCTCCAAGGTC
AGGGTGTTCCTGGACGGCAGGCCGATCAACGACCCGACCTCCAGCACGGGAGGGGTCACCTGGTACCTGGTGACCCCCCA
GGACGTGGAGCGGATCGAGATCATCAGGGACAAGGGGGCCATGAAGTACGGCCAGGACGCCAGCGCCGGGGTGATACTCA
TAACCACCCGCACGGTTCAGACCCTGAGCGGCAACGTCAAGGCCTATGGCGGCAACCAGGAGACCGGCTACGGTCAGGCC
AACCTGCGGTACAGGCGCGGCGGACTGGGGGTGGGGGTCAACGGCGGGGGCGAGACGACCGGAGGGTATACGGTCAACAA
CGACAAGACGCGCTACCAGGGCGGGGGCAGACTCTCCTACGACTTCACGGACAGCCGCTCCCTGGCGCTGGCGGGCAGCT
ACATCCTGGACGAGCGCGGCCTGGCCGGTCAACCCGCGTATCCGACCCCCCATTCCCGGAAGGAGAGCCGCAACCTGGGA
CTCTCCCTGCGGGGCGTGTATGACCGGACGACCAGCACGACCTATTACAACCGGGGGAGCCAGCACAATACCGACGTCAG
CAGGAATCTGGACCAGAAGCTGCGGGTCGCGGAGATGGGGGAGGAACTCTCCACCACCCTGAAGAGCGGCCGTTTCGGGG
AGGCCCAGCTGGGAGGGGCGTTCAAGATGTTCAGGGCCTCGGGGAGCTCCTTCGACGCCACCCACGAGGAGACCTATTCC
CTGTATGCAGCCCAGTCGCTGCGCTGCGCGGAGCTGAGTGATTTCTCCCTCTATGTCGGCGTCAGGGTCAACTTCAACAC
CGCCTTCGACAACTCCTATAATCCGGAGATCAAGCTGGTCTATACGGGAAACAATTGGAACGCCTCCGCCGGGTACGGCC
GGGCCAACAACACCCCCTCCTTCTACCAGCGCTACAACCGGACCAGCTCGACCATCCCCAACCCCTCGCTGGACATGGAG
GTGTCGGACAACTTCAACCTGTCGTTCTTCGCCAGGCCGCACGAGGCCCTCTCCGGCAGCGTCTCCCTGTTCTACAACCG
TCTGAAAGACCGTATCACTTACGTCAGTCTCGGCGGAGTCGGCCAGTATCAGAACTTCGGCCGCGTGACCTACCGGGGGG
GGGATCTGGCCCTGGATTGGAAGCCGCATCAGGCCGTCAATCTGAAAGCCAGTTACATCTACATGGAGGCAAAGGACCAG
GAGAGCGGCAAGACGGTTACCTGCAAGCCGAGGCACACCGTCAAGGCGGATCTCTACCTCAAACCGTTTCAGGCCCTGAC
CGCCGTGATTTCCGGAAAATACACCTCCATGGCCTATATCGACAAGAACAACACCAGGAGTATTCCCGGTTACTTCCTGC
TGGACCTGCGGGGAGAGTACTCCGTCTCCAAAACGATCAGCCTGTTCGCGGAGATAGAAAACCTGCTGGACAAGGACTAC
CTGTACGTGGACGGGCTTCAGGGGCCGTCGCGTACCTGGATCCTGGGGATGAACGTGAAATTTTAG

Upstream 100 bases:

>100_bases
AGACGAGATGCAGGAGGAACTGCGCAACGCCTATCTGGAGATGGAAGGGGAGATCGAGGAATGGACCGAGTAGCCGGCCG
GAAGGACAGGGGGGGCTCAG

Downstream 100 bases:

>100_bases
CCGCGGTTCCCGCTTCCCCGACGTGCGACGGAGGAGCCGGGAACCGGGAAGGGACGATGTGCATATGTACGGAAACGTGT
ACCCGATGGCCGCTCTGGTG

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MPFPDRHGVGGRAFFLCPLIAVVSVLGPVTASAQERDDMIVITADEIAAMKANRMEELLNHVPGVSAGDSSISIHGSSKV
RVFLDGRPINDPTSSTGGVTWYLVTPQDVERIEIIRDKGAMKYGQDASAGVILITTRTVQTLSGNVKAYGGNQETGYGQA
NLRYRRGGLGVGVNGGGETTGGYTVNNDKTRYQGGGRLSYDFTDSRSLALAGSYILDERGLAGQPAYPTPHSRKESRNLG
LSLRGVYDRTTSTTYYNRGSQHNTDVSRNLDQKLRVAEMGEELSTTLKSGRFGEAQLGGAFKMFRASGSSFDATHEETYS
LYAAQSLRCAELSDFSLYVGVRVNFNTAFDNSYNPEIKLVYTGNNWNASAGYGRANNTPSFYQRYNRTSSTIPNPSLDME
VSDNFNLSFFARPHEALSGSVSLFYNRLKDRITYVSLGGVGQYQNFGRVTYRGGDLALDWKPHQAVNLKASYIYMEAKDQ
ESGKTVTCKPRHTVKADLYLKPFQALTAVISGKYTSMAYIDKNNTRSIPGYFLLDLRGEYSVSKTISLFAEIENLLDKDY
LYVDGLQGPSRTWILGMNVKF

Sequences:

>Translated_581_residues
MPFPDRHGVGGRAFFLCPLIAVVSVLGPVTASAQERDDMIVITADEIAAMKANRMEELLNHVPGVSAGDSSISIHGSSKV
RVFLDGRPINDPTSSTGGVTWYLVTPQDVERIEIIRDKGAMKYGQDASAGVILITTRTVQTLSGNVKAYGGNQETGYGQA
NLRYRRGGLGVGVNGGGETTGGYTVNNDKTRYQGGGRLSYDFTDSRSLALAGSYILDERGLAGQPAYPTPHSRKESRNLG
LSLRGVYDRTTSTTYYNRGSQHNTDVSRNLDQKLRVAEMGEELSTTLKSGRFGEAQLGGAFKMFRASGSSFDATHEETYS
LYAAQSLRCAELSDFSLYVGVRVNFNTAFDNSYNPEIKLVYTGNNWNASAGYGRANNTPSFYQRYNRTSSTIPNPSLDME
VSDNFNLSFFARPHEALSGSVSLFYNRLKDRITYVSLGGVGQYQNFGRVTYRGGDLALDWKPHQAVNLKASYIYMEAKDQ
ESGKTVTCKPRHTVKADLYLKPFQALTAVISGKYTSMAYIDKNNTRSIPGYFLLDLRGEYSVSKTISLFAEIENLLDKDY
LYVDGLQGPSRTWILGMNVKF
>Mature_580_residues
PFPDRHGVGGRAFFLCPLIAVVSVLGPVTASAQERDDMIVITADEIAAMKANRMEELLNHVPGVSAGDSSISIHGSSKVR
VFLDGRPINDPTSSTGGVTWYLVTPQDVERIEIIRDKGAMKYGQDASAGVILITTRTVQTLSGNVKAYGGNQETGYGQAN
LRYRRGGLGVGVNGGGETTGGYTVNNDKTRYQGGGRLSYDFTDSRSLALAGSYILDERGLAGQPAYPTPHSRKESRNLGL
SLRGVYDRTTSTTYYNRGSQHNTDVSRNLDQKLRVAEMGEELSTTLKSGRFGEAQLGGAFKMFRASGSSFDATHEETYSL
YAAQSLRCAELSDFSLYVGVRVNFNTAFDNSYNPEIKLVYTGNNWNASAGYGRANNTPSFYQRYNRTSSTIPNPSLDMEV
SDNFNLSFFARPHEALSGSVSLFYNRLKDRITYVSLGGVGQYQNFGRVTYRGGDLALDWKPHQAVNLKASYIYMEAKDQE
SGKTVTCKPRHTVKADLYLKPFQALTAVISGKYTSMAYIDKNNTRSIPGYFLLDLRGEYSVSKTISLFAEIENLLDKDYL
YVDGLQGPSRTWILGMNVKF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4771

COG function: function code P; Outer membrane receptor for ferrienterochelin and colicins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=622, Percent_Identity=23.7942122186495, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 63892; Mature: 63761

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFPDRHGVGGRAFFLCPLIAVVSVLGPVTASAQERDDMIVITADEIAAMKANRMEELLN
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHH
HVPGVSAGDSSISIHGSSKVRVFLDGRPINDPTSSTGGVTWYLVTPQDVERIEIIRDKGA
HCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCC
MKYGQDASAGVILITTRTVQTLSGNVKAYGGNQETGYGQANLRYRRGGLGVGVNGGGETT
CCCCCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCC
GGYTVNNDKTRYQGGGRLSYDFTDSRSLALAGSYILDERGLAGQPAYPTPHSRKESRNLG
CCEEECCCCEEECCCCEEEEECCCCCCEEEECCEEEECCCCCCCCCCCCCCCCCHHCCCC
LSLRGVYDRTTSTTYYNRGSQHNTDVSRNLDQKLRVAEMGEELSTTLKSGRFGEAQLGGA
EEEEEEECCCCCCEEECCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCE
FKMFRASGSSFDATHEETYSLYAAQSLRCAELSDFSLYVGVRVNFNTAFDNSYNPEIKLV
EEEEECCCCCCCCCCHHHEEEEECCCCCEEECCCEEEEEEEEEEEEEECCCCCCCEEEEE
YTGNNWNASAGYGRANNTPSFYQRYNRTSSTIPNPSLDMEVSDNFNLSFFARPHEALSGS
EECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEECCHHHHCCH
VSLFYNRLKDRITYVSLGGVGQYQNFGRVTYRGGDLALDWKPHQAVNLKASYIYMEAKDQ
HHHHHHHHHHCEEEEEECCCCCCCCCCEEEEECCCEEEECCCCCEEEEEEEEEEEECCCC
ESGKTVTCKPRHTVKADLYLKPFQALTAVISGKYTSMAYIDKNNTRSIPGYFLLDLRGEY
CCCCEEEECCCCCEEEEEEECCHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEECCCC
SVSKTISLFAEIENLLDKDYLYVDGLQGPSRTWILGMNVKF
CHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEECC
>Mature Secondary Structure 
PFPDRHGVGGRAFFLCPLIAVVSVLGPVTASAQERDDMIVITADEIAAMKANRMEELLN
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHH
HVPGVSAGDSSISIHGSSKVRVFLDGRPINDPTSSTGGVTWYLVTPQDVERIEIIRDKGA
HCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCC
MKYGQDASAGVILITTRTVQTLSGNVKAYGGNQETGYGQANLRYRRGGLGVGVNGGGETT
CCCCCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCC
GGYTVNNDKTRYQGGGRLSYDFTDSRSLALAGSYILDERGLAGQPAYPTPHSRKESRNLG
CCEEECCCCEEECCCCEEEEECCCCCCEEEECCEEEECCCCCCCCCCCCCCCCCHHCCCC
LSLRGVYDRTTSTTYYNRGSQHNTDVSRNLDQKLRVAEMGEELSTTLKSGRFGEAQLGGA
EEEEEEECCCCCCEEECCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCE
FKMFRASGSSFDATHEETYSLYAAQSLRCAELSDFSLYVGVRVNFNTAFDNSYNPEIKLV
EEEEECCCCCCCCCCHHHEEEEECCCCCEEECCCEEEEEEEEEEEEEECCCCCCCEEEEE
YTGNNWNASAGYGRANNTPSFYQRYNRTSSTIPNPSLDMEVSDNFNLSFFARPHEALSGS
EECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEECCHHHHCCH
VSLFYNRLKDRITYVSLGGVGQYQNFGRVTYRGGDLALDWKPHQAVNLKASYIYMEAKDQ
HHHHHHHHHHCEEEEEECCCCCCCCCCEEEEECCCEEEECCCCCEEEEEEEEEEEECCCC
ESGKTVTCKPRHTVKADLYLKPFQALTAVISGKYTSMAYIDKNNTRSIPGYFLLDLRGEY
CCCCEEEECCCCCEEEEEEECCHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEECCCC
SVSKTISLFAEIENLLDKDYLYVDGLQGPSRTWILGMNVKF
CHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA