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The map label for this gene is btuB [H]

Identifier: 118579666

GI number: 118579666

Start: 1294528

End: 1296738

Strand: Reverse

Name: btuB [H]

Synonym: Ppro_1237

Alternate gene names: 118579666

Gene position: 1296738-1294528 (Counterclockwise)

Preceding gene: 118579667

Following gene: 118579665

Centisome position: 32.35

GC content: 58.62

Gene sequence:

>2211_bases
ATGCAGCAGGAAAATTTCATACGCGCGATTGCCTGCGGTGTGGCGCTGGCGGTTGCGCCGTCTGTCGCAACGGCCCAGGA
ACGGGAAACGGAACAGATGGATACGGTCATCGTGACCGCCACCAGAACCGAAAAGGAGTTGGACTCCGCGCCCGGCAGCG
CGTCCGTGGTCACGAAGCAGGAAATCGAAAAAAAAGAAGTCCTTACCTTTGACGACGCCTTGAAGGGAACGTCCGGTGTC
ATGCTTTCGCGTTCAAAAGGCCCCATGGATACGATAGCGAACATTACCCTGCGCGGCGTTCCCGGACAGAACAGGACGCT
GATCATGGTGGACGGCATCACCCTGAACAGCCCCTTCGCCGGATCGATCCAGTCAAACAGCATTGCCCCCGGCACCATCG
AGAGGATCGAGATTGTCAAGGGGGCAACCTCCAGCCTCTACGGCGGCAACGCCATGGGCGGCGTCGTCAATGTGATCACC
AAAATGCCGACCAAGCGCGAGTTCACCCTCAAGACCGGCTTCGGTTCCGCCCTGGAGGGGGATGCTCCGGAAAACACCCG
GCGTGTGGCGGTCTCCTACGGTGATGTGATTAAAGACAAATTCCATATCTACCTTTACAACGATTACACCGGAACGGACG
GTTACGTAACCGATCTGGTGACCGGCACTCCCGGCAGCGGCACCAGCGGGGCGCGACCGACGCTGACCAGTGACGAAAAG
GCTACCTTTCTGCTGGGCCACAAGGGCGAGAACGAGGCCTGGCAGGACAATCTTACCGTCAAGGCCGGGTATGATTTCAA
TTCCAGCACGAAACTGCTCTTCACCTTCCTCAAGTCCTATGGCGAATACTCCTACAACGATCCCGAGACCTATATGACCG
ACTCCTCGGGAAACCCTTACTGGAGCTCTTCCGGCTCGGAGTACTCCTTCCTGGGGACCTACGGCGCCAACAACCAAAAC
ATCTACGCTCTTTCCCTGGAAACGGAGATAGCCTCCGCCAAGATCAAGGCCGGGATCAGCTACCTGGATCAGGATACCAG
CTGGTATGCCACCGCCAGCAGCACGACCAAATCCCCGGTAGCCACCCGCTCCGACGGTCCGGGCAAACTCACCAGCACCC
CCGCCTCGGCCCTGAGCGCTGACGTGCAGGTAACCTTCCCGCTCCTATCCTGGAACATCTTCACCGTCGGAGGCGCCTTC
CGCCAGTCGGAGATCAACGGCAAGGACTATGTGCTTTCCGACTGGACGAATGAGCACAGCAAGGGGCAGCTTATCGGGGA
AGCCAGGGGAACGGACAGGATCTATGCGCTGTTCATGCAGGACGAAATTTCAATCCTGGATAACCTGACGCTCTACGCCG
GATTCCGCCAGGACTGGTGGGAAACCAGCGACGGCCATGTCCTGGCCTCCAACGCGTCCAACGTCGTCACGGTCGGTCCC
ACGTCATTCGGCTCCCGCAGCGAAAGCGCATTCAGCCCCAAGGGGGCCATCGTCTACACGCCCTTTGCGGGCACCACCCT
GAAGGTCTCCGGCGGCAAGGCTTTCCGCGCTCCCAGCAACTACGAACTCTACCGCACCACCATGATGGGAAGCTCCACCA
CCTACCAGTGCAACCCGGACTTAAAGCCGGAGCGAAGCGTCAGCTGGGACGGCGGCGTAAGCCAGGATCTGTGGCAAGGC
GCAACGGTCAAGGCCACCTATTTTGAGAACTACATCAAGGACATGATCTACAGCACCACCACCGGCAGCATCCGGGAAAA
GAAAAACGCCGGCAGAGGCGAAAGCAAGGGGGTGGAGGTGGAGCTGGAACAGAAATTCGGAAAACTGCTGCGCCTGTTCG
CCAACTACACCTACACCAACGCCAAGATAACCAAGAACAGCGCCGTCCCGGCCACGGAGGGAAAACGCATGACCGATATC
CCCCGCACCATGTACAACGCCGGCGCCGACCTGGAGTATGGGCCCGCAGGCGCGTCGGTCATCGCCCGCTACGTGGGAAA
ACGGTACGGCAACGACACCAACAACGACACTGCCAGGAATGTGAAGGGGGTCTACGACTCCTACATCACCACGGACGTCA
AACTGCGCTACAAACTGACGTCCTGGGCAACGGCATCCTTTTCGGTCAACAACATCTTCGATGAGAAATACTTCTCATCC
ACAAAATCTCCGGGGCGATCATGTTACGGCGAAGTGACGTTACAGTACTGA

Upstream 100 bases:

>100_bases
CGACATCAAGGGTGCCGGCATCGAGCGCCTGGGCATGGTGACGGAACCGGCGAAGCCGTAACGCGGGACAGCACGATTCA
ATTTTAGTGAGGGAGGATGC

Downstream 100 bases:

>100_bases
TCCGGCCAGACCAGTCCTGGCCGGTCTGGTCATCGCTCGTGCTGCACGGGGCGCTGTTCGCCTTCTGCTGCACCCTTCCC
TACACACCAAAGGTGCATGA

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MQQENFIRAIACGVALAVAPSVATAQERETEQMDTVIVTATRTEKELDSAPGSASVVTKQEIEKKEVLTFDDALKGTSGV
MLSRSKGPMDTIANITLRGVPGQNRTLIMVDGITLNSPFAGSIQSNSIAPGTIERIEIVKGATSSLYGGNAMGGVVNVIT
KMPTKREFTLKTGFGSALEGDAPENTRRVAVSYGDVIKDKFHIYLYNDYTGTDGYVTDLVTGTPGSGTSGARPTLTSDEK
ATFLLGHKGENEAWQDNLTVKAGYDFNSSTKLLFTFLKSYGEYSYNDPETYMTDSSGNPYWSSSGSEYSFLGTYGANNQN
IYALSLETEIASAKIKAGISYLDQDTSWYATASSTTKSPVATRSDGPGKLTSTPASALSADVQVTFPLLSWNIFTVGGAF
RQSEINGKDYVLSDWTNEHSKGQLIGEARGTDRIYALFMQDEISILDNLTLYAGFRQDWWETSDGHVLASNASNVVTVGP
TSFGSRSESAFSPKGAIVYTPFAGTTLKVSGGKAFRAPSNYELYRTTMMGSSTTYQCNPDLKPERSVSWDGGVSQDLWQG
ATVKATYFENYIKDMIYSTTTGSIREKKNAGRGESKGVEVELEQKFGKLLRLFANYTYTNAKITKNSAVPATEGKRMTDI
PRTMYNAGADLEYGPAGASVIARYVGKRYGNDTNNDTARNVKGVYDSYITTDVKLRYKLTSWATASFSVNNIFDEKYFSS
TKSPGRSCYGEVTLQY

Sequences:

>Translated_736_residues
MQQENFIRAIACGVALAVAPSVATAQERETEQMDTVIVTATRTEKELDSAPGSASVVTKQEIEKKEVLTFDDALKGTSGV
MLSRSKGPMDTIANITLRGVPGQNRTLIMVDGITLNSPFAGSIQSNSIAPGTIERIEIVKGATSSLYGGNAMGGVVNVIT
KMPTKREFTLKTGFGSALEGDAPENTRRVAVSYGDVIKDKFHIYLYNDYTGTDGYVTDLVTGTPGSGTSGARPTLTSDEK
ATFLLGHKGENEAWQDNLTVKAGYDFNSSTKLLFTFLKSYGEYSYNDPETYMTDSSGNPYWSSSGSEYSFLGTYGANNQN
IYALSLETEIASAKIKAGISYLDQDTSWYATASSTTKSPVATRSDGPGKLTSTPASALSADVQVTFPLLSWNIFTVGGAF
RQSEINGKDYVLSDWTNEHSKGQLIGEARGTDRIYALFMQDEISILDNLTLYAGFRQDWWETSDGHVLASNASNVVTVGP
TSFGSRSESAFSPKGAIVYTPFAGTTLKVSGGKAFRAPSNYELYRTTMMGSSTTYQCNPDLKPERSVSWDGGVSQDLWQG
ATVKATYFENYIKDMIYSTTTGSIREKKNAGRGESKGVEVELEQKFGKLLRLFANYTYTNAKITKNSAVPATEGKRMTDI
PRTMYNAGADLEYGPAGASVIARYVGKRYGNDTNNDTARNVKGVYDSYITTDVKLRYKLTSWATASFSVNNIFDEKYFSS
TKSPGRSCYGEVTLQY
>Mature_736_residues
MQQENFIRAIACGVALAVAPSVATAQERETEQMDTVIVTATRTEKELDSAPGSASVVTKQEIEKKEVLTFDDALKGTSGV
MLSRSKGPMDTIANITLRGVPGQNRTLIMVDGITLNSPFAGSIQSNSIAPGTIERIEIVKGATSSLYGGNAMGGVVNVIT
KMPTKREFTLKTGFGSALEGDAPENTRRVAVSYGDVIKDKFHIYLYNDYTGTDGYVTDLVTGTPGSGTSGARPTLTSDEK
ATFLLGHKGENEAWQDNLTVKAGYDFNSSTKLLFTFLKSYGEYSYNDPETYMTDSSGNPYWSSSGSEYSFLGTYGANNQN
IYALSLETEIASAKIKAGISYLDQDTSWYATASSTTKSPVATRSDGPGKLTSTPASALSADVQVTFPLLSWNIFTVGGAF
RQSEINGKDYVLSDWTNEHSKGQLIGEARGTDRIYALFMQDEISILDNLTLYAGFRQDWWETSDGHVLASNASNVVTVGP
TSFGSRSESAFSPKGAIVYTPFAGTTLKVSGGKAFRAPSNYELYRTTMMGSSTTYQCNPDLKPERSVSWDGGVSQDLWQG
ATVKATYFENYIKDMIYSTTTGSIREKKNAGRGESKGVEVELEQKFGKLLRLFANYTYTNAKITKNSAVPATEGKRMTDI
PRTMYNAGADLEYGPAGASVIARYVGKRYGNDTNNDTARNVKGVYDSYITTDVKLRYKLTSWATASFSVNNIFDEKYFSS
TKSPGRSCYGEVTLQY

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=161, Percent_Identity=39.1304347826087, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790405, Length=213, Percent_Identity=29.1079812206573, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1786344, Length=205, Percent_Identity=27.8048780487805, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786798, Length=232, Percent_Identity=30.1724137931034, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1787723, Length=328, Percent_Identity=24.390243902439, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 79952; Mature: 79952

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQENFIRAIACGVALAVAPSVATAQERETEQMDTVIVTATRTEKELDSAPGSASVVTKQ
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEECCHHHHHCCCCCCEEEEHH
EIEKKEVLTFDDALKGTSGVMLSRSKGPMDTIANITLRGVPGQNRTLIMVDGITLNSPFA
HHCHHHEEEHHHHCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCEEEEECCEEECCCCC
GSIQSNSIAPGTIERIEIVKGATSSLYGGNAMGGVVNVITKMPTKREFTLKTGFGSALEG
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCC
DAPENTRRVAVSYGDVIKDKFHIYLYNDYTGTDGYVTDLVTGTPGSGTSGARPTLTSDEK
CCCCCCEEEEEEECCEECCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
ATFLLGHKGENEAWQDNLTVKAGYDFNSSTKLLFTFLKSYGEYSYNDPETYMTDSSGNPY
EEEEEEECCCCCCHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCC
WSSSGSEYSFLGTYGANNQNIYALSLETEIASAKIKAGISYLDQDTSWYATASSTTKSPV
CCCCCCCEEEEEEECCCCCEEEEEEECCHHHHHHHHHCHHHHCCCCCEEEEECCCCCCCC
ATRSDGPGKLTSTPASALSADVQVTFPLLSWNIFTVGGAFRQSEINGKDYVLSDWTNEHS
CCCCCCCCCCCCCCHHHHCCCEEEEEEEEEEEEEEECCCEEECCCCCCCEEEECCCCCCC
KGQLIGEARGTDRIYALFMQDEISILDNLTLYAGFRQDWWETSDGHVLASNASNVVTVGP
CCCEEECCCCCCEEEEEEEECCHHHHHCEEEEECCCHHHCCCCCCEEEECCCCCEEEECC
TSFGSRSESAFSPKGAIVYTPFAGTTLKVSGGKAFRAPSNYELYRTTMMGSSTTYQCNPD
CCCCCCCCCCCCCCCCEEECCCCCCEEEECCCEEECCCCCCEEEEEEEECCCCEEEECCC
LKPERSVSWDGGVSQDLWQGATVKATYFENYIKDMIYSTTTGSIREKKNAGRGESKGVEV
CCCCCCCCCCCCCCHHHHCCCEEEHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEE
ELEQKFGKLLRLFANYTYTNAKITKNSAVPATEGKRMTDIPRTMYNAGADLEYGPAGASV
EHHHHHHHHHHHHHCCEECCEEEECCCCCCCCCCCCCHHCCHHHHHCCCCCCCCCCHHHH
IARYVGKRYGNDTNNDTARNVKGVYDSYITTDVKLRYKLTSWATASFSVNNIFDEKYFSS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEEEEEEEEECEEEEEEEHHHHHHHHHHHC
TKSPGRSCYGEVTLQY
CCCCCCCCEEEEEEEC
>Mature Secondary Structure
MQQENFIRAIACGVALAVAPSVATAQERETEQMDTVIVTATRTEKELDSAPGSASVVTKQ
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEECCHHHHHCCCCCCEEEEHH
EIEKKEVLTFDDALKGTSGVMLSRSKGPMDTIANITLRGVPGQNRTLIMVDGITLNSPFA
HHCHHHEEEHHHHCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCEEEEECCEEECCCCC
GSIQSNSIAPGTIERIEIVKGATSSLYGGNAMGGVVNVITKMPTKREFTLKTGFGSALEG
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCC
DAPENTRRVAVSYGDVIKDKFHIYLYNDYTGTDGYVTDLVTGTPGSGTSGARPTLTSDEK
CCCCCCEEEEEEECCEECCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
ATFLLGHKGENEAWQDNLTVKAGYDFNSSTKLLFTFLKSYGEYSYNDPETYMTDSSGNPY
EEEEEEECCCCCCHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCC
WSSSGSEYSFLGTYGANNQNIYALSLETEIASAKIKAGISYLDQDTSWYATASSTTKSPV
CCCCCCCEEEEEEECCCCCEEEEEEECCHHHHHHHHHCHHHHCCCCCEEEEECCCCCCCC
ATRSDGPGKLTSTPASALSADVQVTFPLLSWNIFTVGGAFRQSEINGKDYVLSDWTNEHS
CCCCCCCCCCCCCCHHHHCCCEEEEEEEEEEEEEEECCCEEECCCCCCCEEEECCCCCCC
KGQLIGEARGTDRIYALFMQDEISILDNLTLYAGFRQDWWETSDGHVLASNASNVVTVGP
CCCEEECCCCCCEEEEEEEECCHHHHHCEEEEECCCHHHCCCCCCEEEECCCCCEEEECC
TSFGSRSESAFSPKGAIVYTPFAGTTLKVSGGKAFRAPSNYELYRTTMMGSSTTYQCNPD
CCCCCCCCCCCCCCCCEEECCCCCCEEEECCCEEECCCCCCEEEEEEEECCCCEEEECCC
LKPERSVSWDGGVSQDLWQGATVKATYFENYIKDMIYSTTTGSIREKKNAGRGESKGVEV
CCCCCCCCCCCCCCHHHHCCCEEEHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEE
ELEQKFGKLLRLFANYTYTNAKITKNSAVPATEGKRMTDIPRTMYNAGADLEYGPAGASV
EHHHHHHHHHHHHHCCEECCEEEECCCCCCCCCCCCCHHCCHHHHHCCCCCCCCCCHHHH
IARYVGKRYGNDTNNDTARNVKGVYDSYITTDVKLRYKLTSWATASFSVNNIFDEKYFSS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEEEEEEEEECEEEEEEEHHHHHHHHHHHC
TKSPGRSCYGEVTLQY
CCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA