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The map label for this gene is dfrA [H]

Identifier: 118579620

GI number: 118579620

Start: 1241699

End: 1242766

Strand: Direct

Name: dfrA [H]

Synonym: Ppro_1190

Alternate gene names: 118579620

Gene position: 1241699-1242766 (Clockwise)

Preceding gene: 118579619

Following gene: 118579621

Centisome position: 30.98

GC content: 63.58

Gene sequence:

>1068_bases
TTGAGCGGCCTGTTTTGGTACTACCGCGATTGTCCGCGGCAATCCCGTCCACGGCTGGTCCGTGCGTCGGATACACAGGA
GCATATGAAGACTTTCATCACCGGCGCCACCGGGTTCATAGGCGCAAGTATTGTCAGGGAACTATTGAAGGAGGGGCGCC
AGGTGCGCGCCCTGGTGCGCCCCAGTTCGGATACCTCCAACCTGGCCGGACTGGACGTGGAGTTCTGGAAGGGGGACCTG
CGCGACCGGGACAGCCTGGTGAGCGGTTTGAAGGGGTGCGACGTGCTTTACCACGCGGCCGCCGATTACCGCCTCTGGAC
GCGCAACCCCGCCGAGATGTACCGCATCAACGTGGATGGCACGGCCGCCATTCTGGACGCTGCCCTGAAAAACGGCCTCT
CCCGCGTGGTCTACACCAGCAGCGTGGGGACCCTGGGCAATCCGGGGGACGGCACTCCGGGAAGCGAGGATGCGCCGGTC
ACCCTGAACGACATGGTCGGACATTACAAGAAGAGCAAGTTCCTGGCCGAGCGCGAGGCGGATACGTTCGTGGCCAGGGG
GTTGCCGTTGGTCATCGTCAATCCCTCAACCCCCATCGGCCCTCTGGACATCAAGCCGACCCCCACCGGCAAGATCATCG
TCGACTTCCTGAACCGCAGGATGCCGGCCTATCTGGATACGGGACTCAACATCATCGCGGTGGAGGATTGCGCCCGTGGC
CACCTCTTGGCGGAGAAGCGGGGCCAGGTGGGCAGAAAGTACATCCTGGGCAACAGCAACCTGACCCTGGCCGACATCTT
CAAGCTGCTGAACAGGATAACCGGACTGCCCGCCCCCCGGTTGCGGCTTCCCTATACGCCGATCCTGCTGGCCGCTTATG
TCAATGAGGGACTCTCCCGGATTACGGGCAGGGAACCGCTGATCCCTCTGGCCGGGGTGCAGATGGCGGCGAAGTTCATG
TTCTTCGACTCATCCCGTGCGGTCAGGGAGCTGGGGCTGCCCCAGTCGTCGGTGGAGGATGCCCTGCGGCGCGCCGTGGA
GTGGTTCAGGGCCAACCGGTATGCGTGA

Upstream 100 bases:

>100_bases
CAGGCTGACCGCCGCCAAGGGGTAAGGCCGCAGGGCAGCACATTCGGCATTTATTTGCAGGAGGCCCCGAGTTCGCCGCA
ATCCGGGGCTTTCCCCTTTT

Downstream 100 bases:

>100_bases
CGTCAGCCGGCGATTGCTCGCAACCACTGCCGATTGAAACGCGCCCGACTGGCCTGTGATGGAGAAACAGTAAAATGTAT
CAGTATTTGAACCATATAGA

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSGLFWYYRDCPRQSRPRLVRASDTQEHMKTFITGATGFIGASIVRELLKEGRQVRALVRPSSDTSNLAGLDVEFWKGDL
RDRDSLVSGLKGCDVLYHAAADYRLWTRNPAEMYRINVDGTAAILDAALKNGLSRVVYTSSVGTLGNPGDGTPGSEDAPV
TLNDMVGHYKKSKFLAEREADTFVARGLPLVIVNPSTPIGPLDIKPTPTGKIIVDFLNRRMPAYLDTGLNIIAVEDCARG
HLLAEKRGQVGRKYILGNSNLTLADIFKLLNRITGLPAPRLRLPYTPILLAAYVNEGLSRITGREPLIPLAGVQMAAKFM
FFDSSRAVRELGLPQSSVEDALRRAVEWFRANRYA

Sequences:

>Translated_355_residues
MSGLFWYYRDCPRQSRPRLVRASDTQEHMKTFITGATGFIGASIVRELLKEGRQVRALVRPSSDTSNLAGLDVEFWKGDL
RDRDSLVSGLKGCDVLYHAAADYRLWTRNPAEMYRINVDGTAAILDAALKNGLSRVVYTSSVGTLGNPGDGTPGSEDAPV
TLNDMVGHYKKSKFLAEREADTFVARGLPLVIVNPSTPIGPLDIKPTPTGKIIVDFLNRRMPAYLDTGLNIIAVEDCARG
HLLAEKRGQVGRKYILGNSNLTLADIFKLLNRITGLPAPRLRLPYTPILLAAYVNEGLSRITGREPLIPLAGVQMAAKFM
FFDSSRAVRELGLPQSSVEDALRRAVEWFRANRYA
>Mature_354_residues
SGLFWYYRDCPRQSRPRLVRASDTQEHMKTFITGATGFIGASIVRELLKEGRQVRALVRPSSDTSNLAGLDVEFWKGDLR
DRDSLVSGLKGCDVLYHAAADYRLWTRNPAEMYRINVDGTAAILDAALKNGLSRVVYTSSVGTLGNPGDGTPGSEDAPVT
LNDMVGHYKKSKFLAEREADTFVARGLPLVIVNPSTPIGPLDIKPTPTGKIIVDFLNRRMPAYLDTGLNIIAVEDCARGH
LLAEKRGQVGRKYILGNSNLTLADIFKLLNRITGLPAPRLRLPYTPILLAAYVNEGLSRITGREPLIPLAGVQMAAKFMF
FDSSRAVRELGLPQSSVEDALRRAVEWFRANRYA

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=340, Percent_Identity=26.1764705882353, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI8393516, Length=340, Percent_Identity=26.1764705882353, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI310132178, Length=335, Percent_Identity=24.4776119402985, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI310113012, Length=335, Percent_Identity=24.4776119402985, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI239745448, Length=335, Percent_Identity=24.4776119402985, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI116268111, Length=351, Percent_Identity=24.5014245014245, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71987463, Length=336, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 39164; Mature: 39032

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLFWYYRDCPRQSRPRLVRASDTQEHMKTFITGATGFIGASIVRELLKEGRQVRALVR
CCCCEEHHHCCCCCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHEEEEEC
PSSDTSNLAGLDVEFWKGDLRDRDSLVSGLKGCDVLYHAAADYRLWTRNPAEMYRINVDG
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCHHHHHHHHCCCCEEEECCCCCEEEEECCC
TAAILDAALKNGLSRVVYTSSVGTLGNPGDGTPGSEDAPVTLNDMVGHYKKSKFLAEREA
HHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
DTFVARGLPLVIVNPSTPIGPLDIKPTPTGKIIVDFLNRRMPAYLDTGLNIIAVEDCARG
HHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCEEEEECCCCCC
HLLAEKRGQVGRKYILGNSNLTLADIFKLLNRITGLPAPRLRLPYTPILLAAYVNEGLSR
CHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
ITGREPLIPLAGVQMAAKFMFFDSSRAVRELGLPQSSVEDALRRAVEWFRANRYA
HCCCCCCCCHHHHHHHHHHHEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGLFWYYRDCPRQSRPRLVRASDTQEHMKTFITGATGFIGASIVRELLKEGRQVRALVR
CCCEEHHHCCCCCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHEEEEEC
PSSDTSNLAGLDVEFWKGDLRDRDSLVSGLKGCDVLYHAAADYRLWTRNPAEMYRINVDG
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCHHHHHHHHCCCCEEEECCCCCEEEEECCC
TAAILDAALKNGLSRVVYTSSVGTLGNPGDGTPGSEDAPVTLNDMVGHYKKSKFLAEREA
HHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
DTFVARGLPLVIVNPSTPIGPLDIKPTPTGKIIVDFLNRRMPAYLDTGLNIIAVEDCARG
HHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCEEEEECCCCCC
HLLAEKRGQVGRKYILGNSNLTLADIFKLLNRITGLPAPRLRLPYTPILLAAYVNEGLSR
CHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
ITGREPLIPLAGVQMAAKFMFFDSSRAVRELGLPQSSVEDALRRAVEWFRANRYA
HCCCCCCCCHHHHHHHHHHHEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]