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The map label for this gene is purF [H]

Identifier: 118579584

GI number: 118579584

Start: 1201603

End: 1203027

Strand: Direct

Name: purF [H]

Synonym: Ppro_1153

Alternate gene names: 118579584

Gene position: 1201603-1203027 (Clockwise)

Preceding gene: 118579583

Following gene: 118579585

Centisome position: 29.98

GC content: 61.82

Gene sequence:

>1425_bases
ATGAATTTTACCCGGCCACACGAAGAATGCGGCGTTTTCGGCATCTATAACCACAAGGAAGCGGCCAACCTGACCTATCT
GGGGCTGTATGCCCTGCAGCACCGCGGACAGGAGAGCTGCGGCATCGTCTCCTCCGACGGCCTGCACCTGCACGCCCACA
AGCGCATGGGGCTGGTTGCCGACGTGTTCGGCAACCCGGCGGTGTTCAAGAAGCTGCCCGGCAGCGCGGCCATCGGCCAT
GTGCGCTATTCCACCGCCGGGGCATCGGTGGAGAAGAACGTCCAGCCGATCATGGTGGACTACTCCCGCGGCTCCATCGC
CGTGGCCCACAACGGCAACCTGGTCAATGCCCAGATTCTCAAGGCCGAGTTGGAGGCCTACGGCTCCATTTTCCAGACCA
CCATGGATACGGAGATCATCATCCACCTCTTGGCCGTGGCCCGCACCAACTCCCTGGTGGATCGCATCGTGGAGGCGCTC
AACCGTATCAAGGGCGCCTACTGCCTGCTGTTTCTGACCAAGAGCCGCATGATCGCGGTGCGCGACCCCAATGGGTTCCG
TCCGCTCTGCCTGGGCAAGCTTGGTAACGCCTGGGTGGTTGCTTCGGAAAGCTGCGCCCTGGACCTGATCGAGGCAACCT
TCGTGCGCGAGATCGAACCGGGCGAGATGGTCATCTGCACTAGGGATGGCGGGATTAAATCGCTGTTCCCCTTCAAGAAG
GTGGCGCCGACCCCCTGCATTTTCGAGTTCGTCTACTTCGCCCGTCCCGATTCCCAGATCTTCGGCAAGAACGTCTACCT
GATCCGCAAGGAACTGGGCCGCCAGCTGGCCCGGGAGTACCCGGTGGAGGCGGATGTGGTCATCTCCGTGCCCGACTCGG
GCATGCCGGCCGCCATGGGCTATGCCGAGGAGGCGGGCATACCGTTCGAACTGGGGTTGATCCGCAATCACTACGTGGGC
AGGACCTTTATCGAGCCGGCCCAGTCAATCCGCCACTTCGGGGTCAAGATCAAGCTCAACCCGGTCAAGGAACTGCTCAA
GGGGAAGCGGGTGGTGGTGATCGACGACTCCATCGTGCGGGGAACCACCTCGCGCAAGATCGTCAAGATGGTGCGCCAGG
CCGGGGCCACGGAGGTGCACATGCGCATCTCTTCGCCCCCCACCAGCTATCCTTGCTACTACGGCATCGATACCCCCAAC
CGCAAGGAGCTGATCTCTTCCTCCCATACCGTGGAGGAGGTCTGCCGTTACATCACCGCCGATTCCCTGGGCTACCTGTC
GTCGGAGGGGCTGGTGGAGGCGGTGGGGCTGAGCAACGAGGTCTGCAAGGCCTGTTTCTCCGGGGCGTACCCGATCGCCT
TTCCGAAACCGATGGACAATAACCAGAGAGAGCTGTTCGAAGAGGGAGACGCCCATAATGAATGA

Upstream 100 bases:

>100_bases
CGGAAGAGGGGGACGGGTTGATCCTGTACAAGAACGCGGCCGAGTATGTGCGCAAAAATCTGGTGTAGTCCGTTGTTTTC
AAGCCTAAGGAGCATCTCCC

Downstream 100 bases:

>100_bases
CCGTGAACGACTGAAACAGATTATCCAGGAGCTTTCCTATGAAAACCGGAAGGTGACCCTGGCCTCGGGGCGGGAGAGCG
ATTTCTACTTCGACGGCAAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MNFTRPHEECGVFGIYNHKEAANLTYLGLYALQHRGQESCGIVSSDGLHLHAHKRMGLVADVFGNPAVFKKLPGSAAIGH
VRYSTAGASVEKNVQPIMVDYSRGSIAVAHNGNLVNAQILKAELEAYGSIFQTTMDTEIIIHLLAVARTNSLVDRIVEAL
NRIKGAYCLLFLTKSRMIAVRDPNGFRPLCLGKLGNAWVVASESCALDLIEATFVREIEPGEMVICTRDGGIKSLFPFKK
VAPTPCIFEFVYFARPDSQIFGKNVYLIRKELGRQLAREYPVEADVVISVPDSGMPAAMGYAEEAGIPFELGLIRNHYVG
RTFIEPAQSIRHFGVKIKLNPVKELLKGKRVVVIDDSIVRGTTSRKIVKMVRQAGATEVHMRISSPPTSYPCYYGIDTPN
RKELISSSHTVEEVCRYITADSLGYLSSEGLVEAVGLSNEVCKACFSGAYPIAFPKPMDNNQRELFEEGDAHNE

Sequences:

>Translated_474_residues
MNFTRPHEECGVFGIYNHKEAANLTYLGLYALQHRGQESCGIVSSDGLHLHAHKRMGLVADVFGNPAVFKKLPGSAAIGH
VRYSTAGASVEKNVQPIMVDYSRGSIAVAHNGNLVNAQILKAELEAYGSIFQTTMDTEIIIHLLAVARTNSLVDRIVEAL
NRIKGAYCLLFLTKSRMIAVRDPNGFRPLCLGKLGNAWVVASESCALDLIEATFVREIEPGEMVICTRDGGIKSLFPFKK
VAPTPCIFEFVYFARPDSQIFGKNVYLIRKELGRQLAREYPVEADVVISVPDSGMPAAMGYAEEAGIPFELGLIRNHYVG
RTFIEPAQSIRHFGVKIKLNPVKELLKGKRVVVIDDSIVRGTTSRKIVKMVRQAGATEVHMRISSPPTSYPCYYGIDTPN
RKELISSSHTVEEVCRYITADSLGYLSSEGLVEAVGLSNEVCKACFSGAYPIAFPKPMDNNQRELFEEGDAHNE
>Mature_474_residues
MNFTRPHEECGVFGIYNHKEAANLTYLGLYALQHRGQESCGIVSSDGLHLHAHKRMGLVADVFGNPAVFKKLPGSAAIGH
VRYSTAGASVEKNVQPIMVDYSRGSIAVAHNGNLVNAQILKAELEAYGSIFQTTMDTEIIIHLLAVARTNSLVDRIVEAL
NRIKGAYCLLFLTKSRMIAVRDPNGFRPLCLGKLGNAWVVASESCALDLIEATFVREIEPGEMVICTRDGGIKSLFPFKK
VAPTPCIFEFVYFARPDSQIFGKNVYLIRKELGRQLAREYPVEADVVISVPDSGMPAAMGYAEEAGIPFELGLIRNHYVG
RTFIEPAQSIRHFGVKIKLNPVKELLKGKRVVVIDDSIVRGTTSRKIVKMVRQAGATEVHMRISSPPTSYPCYYGIDTPN
RKELISSSHTVEEVCRYITADSLGYLSSEGLVEAVGLSNEVCKACFSGAYPIAFPKPMDNNQRELFEEGDAHNE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=40.6311637080868, Blast_Score=352, Evalue=4e-97,
Organism=Escherichia coli, GI1788651, Length=467, Percent_Identity=39.186295503212, Blast_Score=295, Evalue=5e-81,
Organism=Escherichia coli, GI1790167, Length=223, Percent_Identity=28.2511210762332, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=42.7974947807933, Blast_Score=356, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6323958, Length=473, Percent_Identity=36.7864693446089, Blast_Score=252, Evalue=8e-68,
Organism=Drosophila melanogaster, GI28573187, Length=495, Percent_Identity=45.4545454545455, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24659598, Length=492, Percent_Identity=44.1056910569106, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24659604, Length=468, Percent_Identity=44.6581196581197, Blast_Score=374, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52188; Mature: 52188

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFTRPHEECGVFGIYNHKEAANLTYLGLYALQHRGQESCGIVSSDGLHLHAHKRMGLVA
CCCCCCCHHCCEEEEECCCCCCCEEEHHHHHHHHCCCCCCCEEECCCEEEEECCCCCEEE
DVFGNPAVFKKLPGSAAIGHVRYSTAGASVEKNVQPIMVDYSRGSIAVAHNGNLVNAQIL
HHCCCCHHHHHCCCCCEECEEEEECCCCCHHCCCCEEEEEECCCCEEEEECCCEEEHHHH
KAELEAYGSIFQTTMDTEIIIHLLAVARTNSLVDRIVEALNRIKGAYCLLFLTKSRMIAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCEEEE
RDPNGFRPLCLGKLGNAWVVASESCALDLIEATFVREIEPGEMVICTRDGGIKSLFPFKK
ECCCCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHH
VAPTPCIFEFVYFARPDSQIFGKNVYLIRKELGRQLAREYPVEADVVISVPDSGMPAAMG
CCCCHHHHHHHHHCCCCHHHHCCCCEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHCC
YAEEAGIPFELGLIRNHYVGRTFIEPAQSIRHFGVKIKLNPVKELLKGKRVVVIDDSIVR
CHHHCCCCEEEEEHHCCCCCCHHHHHHHHHHHCCEEEEECHHHHHHCCCEEEEECCCHHC
GTTSRKIVKMVRQAGATEVHMRISSPPTSYPCYYGIDTPNRKELISSSHTVEEVCRYITA
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHCCCCCHHHHHHHHHH
DSLGYLSSEGLVEAVGLSNEVCKACFSGAYPIAFPKPMDNNQRELFEEGDAHNE
CCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MNFTRPHEECGVFGIYNHKEAANLTYLGLYALQHRGQESCGIVSSDGLHLHAHKRMGLVA
CCCCCCCHHCCEEEEECCCCCCCEEEHHHHHHHHCCCCCCCEEECCCEEEEECCCCCEEE
DVFGNPAVFKKLPGSAAIGHVRYSTAGASVEKNVQPIMVDYSRGSIAVAHNGNLVNAQIL
HHCCCCHHHHHCCCCCEECEEEEECCCCCHHCCCCEEEEEECCCCEEEEECCCEEEHHHH
KAELEAYGSIFQTTMDTEIIIHLLAVARTNSLVDRIVEALNRIKGAYCLLFLTKSRMIAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCEEEE
RDPNGFRPLCLGKLGNAWVVASESCALDLIEATFVREIEPGEMVICTRDGGIKSLFPFKK
ECCCCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHH
VAPTPCIFEFVYFARPDSQIFGKNVYLIRKELGRQLAREYPVEADVVISVPDSGMPAAMG
CCCCHHHHHHHHHCCCCHHHHCCCCEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHCC
YAEEAGIPFELGLIRNHYVGRTFIEPAQSIRHFGVKIKLNPVKELLKGKRVVVIDDSIVR
CHHHCCCCEEEEEHHCCCCCCHHHHHHHHHHHCCEEEEECHHHHHHCCCEEEEECCCHHC
GTTSRKIVKMVRQAGATEVHMRISSPPTSYPCYYGIDTPNRKELISSSHTVEEVCRYITA
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHCCCCCHHHHHHHHHH
DSLGYLSSEGLVEAVGLSNEVCKACFSGAYPIAFPKPMDNNQRELFEEGDAHNE
CCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]