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The map label for this gene is thiC [H]

Identifier: 118579468

GI number: 118579468

Start: 1083519

End: 1085297

Strand: Direct

Name: thiC [H]

Synonym: Ppro_1034

Alternate gene names: 118579468

Gene position: 1083519-1085297 (Clockwise)

Preceding gene: 118579467

Following gene: 118579469

Centisome position: 27.03

GC content: 63.46

Gene sequence:

>1779_bases
ATGAACGAGAACAGGAACTGCCCCAGGGGCACGAACAGCGCGGAGAGCGCGGTAACGGGGGAGTTTCCCCATTCCCGGAG
GGCCTATCTGACCGGTTCGCGGCCTGACCTGCGGGTGCCGATGAGGGAAATCCTCCTCACCGACTCTCCAGGGGGGGATG
GAGCGGCTAAGCCCGCTTCGATCCATGTCTACGACACAGCCGGCCCCTATGGCGATGCCACGCTTTGCTGCGATATCCGC
TCAGGCTTGGCCGGCCTGCGCGAGAACTGGATTGCCGAACGCTACGACACTGAAACGTATTCGGATCCGGCACTTCTGTC
GGAAAAGAGCAACTGTGCCGGCCGCGGGTCTGGCGCTGGCGCCTTTCCCGTCAGCCGCGCCCCCCGCCGCTCCCGCTCTG
GCGGCAACGTGACCCAGATGCACTATGCCCGCCAGGGCATTGTCACCCCCGAGATGGAGTTTGTCGCCATTCGGGAAAAC
CAGCGCCGGGAGGGAGCGGATACGCAACAGCAGACTCCTCATCCCGTTCGTGAGGGGGGCATGTCACGCCCCGCAGTAAT
CACCCCGGAATACGTCAGGTCCGAAGTTGCCCGCGGCAGGGCCATTATTCCGCTCAATATCAACCACCCGGAGGCTGAGC
CGATGATCATCGGCCGTAACTTCCTGGTTAAGGTCAACGCCAATATCGGCAATTCCGCCCTGGCCTCCTCTGTCATGGAC
GAGGTGGAAAAGATGATCTGGGCCATCCGCTGGGGTGCGGATACGGTGATGGACCTCTCCACCGGCGCCCACATCCACGA
GACGCGCGAGTGGATACTGCGCAACAGCCCGGTTCCCATTGGCACGGTGCCCATCTACCAGGCCCTGGAGAAGGTAGCCG
GCGTGGCCGAGGATCTCAGCTGGGACGTCTTCCGCGATACCCTGGTGGAGCAGGCGGAGCAGGGGGTGGACTACTTCACC
ATCCATGCCGGGGTGAGGCTGGAGCATCTGCCGCTGACCTCGCGACGCCTGACCGGCATCGTGTCCCGCGGCGGCTCGAT
CATGGCCAAATGGTGTCATGCCCACAGATGCGAAAGTTTCCTGTACACCCGCTTCGAGGAGATCTGCGAGATCATGAAGG
CCTACGACGTCAGCTTCTCCCTGGGGGATGGCATGCGTCCCGGCTCGCTGCACGATGCCAATGACGAGGCCCAGTTCGCC
GAACTGAAGACCCTGGGCGAGCTGACCCGGCTGGCCTGGAAGCATGACGTGCAGACCATGATAGAGGGGCCGGGGCATGT
GCCCCTGCACCTGATCAGGGAAAACATGGAGCTGCAGCTGGAGCAGTGCCACGAAGCGCCGTTCTATACCCTGGGGCCGC
TGGTCACCGATGTGGCGCCGGGATACGACCACATCACCTCGGCCATCGGCGGGGCCATGATCGGCTGGCTGGGCACCTCC
ATGCTCTGCTACGTCACCCGCAAGGAGCACCTGGGCCTGCCGGACAAAGATGATGTCAAGGAGGGGATTGTCACCTTCAA
GATCGCCGCCCATGCCGCCGACCTGGCAAAGGGGCATCCCGGCGCCCGACTGCGGGACGATGCCCTCTCCAAGGCGCGCT
TTGAATTCCGCTGGAAGGACCAGTTCAACCTGGCCCTCGACCCGGAAATGCCGCAACGCCTGCACGACCTGACCCTGCCC
GCCGAGGCTGACAAGGCATCCCATTTCTGCTCCATGTGCGGCCCGGATTTCTGCGCCATGAGAATCACGCGCAATATCCG
TGAACAGGCGGGAGCGTAA

Upstream 100 bases:

>100_bases
AAAGGCGGCAGGGTCCGGTCGAACACTATTAAAGGAGAGTATCCATGGGGACGGGACGACGGCGGGGATGTCGATTCGGT
CACGGGAGGAGATAGCACGC

Downstream 100 bases:

>100_bases
CAACGCACAACGGAGTCGTGATGTCCCACGCTCATATTCACAAGCATCTGCTGATCAACTCCTACGGCTCCTGGCTGCGC
CGCCACTTCGGCTGCCGGGT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MNENRNCPRGTNSAESAVTGEFPHSRRAYLTGSRPDLRVPMREILLTDSPGGDGAAKPASIHVYDTAGPYGDATLCCDIR
SGLAGLRENWIAERYDTETYSDPALLSEKSNCAGRGSGAGAFPVSRAPRRSRSGGNVTQMHYARQGIVTPEMEFVAIREN
QRREGADTQQQTPHPVREGGMSRPAVITPEYVRSEVARGRAIIPLNINHPEAEPMIIGRNFLVKVNANIGNSALASSVMD
EVEKMIWAIRWGADTVMDLSTGAHIHETREWILRNSPVPIGTVPIYQALEKVAGVAEDLSWDVFRDTLVEQAEQGVDYFT
IHAGVRLEHLPLTSRRLTGIVSRGGSIMAKWCHAHRCESFLYTRFEEICEIMKAYDVSFSLGDGMRPGSLHDANDEAQFA
ELKTLGELTRLAWKHDVQTMIEGPGHVPLHLIRENMELQLEQCHEAPFYTLGPLVTDVAPGYDHITSAIGGAMIGWLGTS
MLCYVTRKEHLGLPDKDDVKEGIVTFKIAAHAADLAKGHPGARLRDDALSKARFEFRWKDQFNLALDPEMPQRLHDLTLP
AEADKASHFCSMCGPDFCAMRITRNIREQAGA

Sequences:

>Translated_592_residues
MNENRNCPRGTNSAESAVTGEFPHSRRAYLTGSRPDLRVPMREILLTDSPGGDGAAKPASIHVYDTAGPYGDATLCCDIR
SGLAGLRENWIAERYDTETYSDPALLSEKSNCAGRGSGAGAFPVSRAPRRSRSGGNVTQMHYARQGIVTPEMEFVAIREN
QRREGADTQQQTPHPVREGGMSRPAVITPEYVRSEVARGRAIIPLNINHPEAEPMIIGRNFLVKVNANIGNSALASSVMD
EVEKMIWAIRWGADTVMDLSTGAHIHETREWILRNSPVPIGTVPIYQALEKVAGVAEDLSWDVFRDTLVEQAEQGVDYFT
IHAGVRLEHLPLTSRRLTGIVSRGGSIMAKWCHAHRCESFLYTRFEEICEIMKAYDVSFSLGDGMRPGSLHDANDEAQFA
ELKTLGELTRLAWKHDVQTMIEGPGHVPLHLIRENMELQLEQCHEAPFYTLGPLVTDVAPGYDHITSAIGGAMIGWLGTS
MLCYVTRKEHLGLPDKDDVKEGIVTFKIAAHAADLAKGHPGARLRDDALSKARFEFRWKDQFNLALDPEMPQRLHDLTLP
AEADKASHFCSMCGPDFCAMRITRNIREQAGA
>Mature_592_residues
MNENRNCPRGTNSAESAVTGEFPHSRRAYLTGSRPDLRVPMREILLTDSPGGDGAAKPASIHVYDTAGPYGDATLCCDIR
SGLAGLRENWIAERYDTETYSDPALLSEKSNCAGRGSGAGAFPVSRAPRRSRSGGNVTQMHYARQGIVTPEMEFVAIREN
QRREGADTQQQTPHPVREGGMSRPAVITPEYVRSEVARGRAIIPLNINHPEAEPMIIGRNFLVKVNANIGNSALASSVMD
EVEKMIWAIRWGADTVMDLSTGAHIHETREWILRNSPVPIGTVPIYQALEKVAGVAEDLSWDVFRDTLVEQAEQGVDYFT
IHAGVRLEHLPLTSRRLTGIVSRGGSIMAKWCHAHRCESFLYTRFEEICEIMKAYDVSFSLGDGMRPGSLHDANDEAQFA
ELKTLGELTRLAWKHDVQTMIEGPGHVPLHLIRENMELQLEQCHEAPFYTLGPLVTDVAPGYDHITSAIGGAMIGWLGTS
MLCYVTRKEHLGLPDKDDVKEGIVTFKIAAHAADLAKGHPGARLRDDALSKARFEFRWKDQFNLALDPEMPQRLHDLTLP
AEADKASHFCSMCGPDFCAMRITRNIREQAGA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=576, Percent_Identity=65.625, Blast_Score=798, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 65417; Mature: 65417

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENRNCPRGTNSAESAVTGEFPHSRRAYLTGSRPDLRVPMREILLTDSPGGDGAAKPAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHEEECCCCCCCCCCCCE
IHVYDTAGPYGDATLCCDIRSGLAGLRENWIAERYDTETYSDPALLSEKSNCAGRGSGAG
EEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCC
AFPVSRAPRRSRSGGNVTQMHYARQGIVTPEMEFVAIRENQRREGADTQQQTPHPVREGG
CCCCCCCCCCCCCCCCEEHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHCC
MSRPAVITPEYVRSEVARGRAIIPLNINHPEAEPMIIGRNFLVKVNANIGNSALASSVMD
CCCCEEECHHHHHHHHHCCCEEEEEECCCCCCCCEEECCEEEEEEECCCCCHHHHHHHHH
EVEKMIWAIRWGADTVMDLSTGAHIHETREWILRNSPVPIGTVPIYQALEKVAGVAEDLS
HHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC
WDVFRDTLVEQAEQGVDYFTIHAGVRLEHLPLTSRRLTGIVSRGGSIMAKWCHAHRCESF
HHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
LYTRFEEICEIMKAYDVSFSLGDGMRPGSLHDANDEAQFAELKTLGELTRLAWKHDVQTM
HHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IEGPGHVPLHLIRENMELQLEQCHEAPFYTLGPLVTDVAPGYDHITSAIGGAMIGWLGTS
HCCCCCCCHHHHHCCHHHHHHHHCCCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
MLCYVTRKEHLGLPDKDDVKEGIVTFKIAAHAADLAKGHPGARLRDDALSKARFEFRWKD
HEEEEECHHHCCCCCHHHHHHCCEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHEEEEECC
QFNLALDPEMPQRLHDLTLPAEADKASHFCSMCGPDFCAMRITRNIREQAGA
CCCEEECCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNENRNCPRGTNSAESAVTGEFPHSRRAYLTGSRPDLRVPMREILLTDSPGGDGAAKPAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHEEECCCCCCCCCCCCE
IHVYDTAGPYGDATLCCDIRSGLAGLRENWIAERYDTETYSDPALLSEKSNCAGRGSGAG
EEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCC
AFPVSRAPRRSRSGGNVTQMHYARQGIVTPEMEFVAIRENQRREGADTQQQTPHPVREGG
CCCCCCCCCCCCCCCCEEHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHCC
MSRPAVITPEYVRSEVARGRAIIPLNINHPEAEPMIIGRNFLVKVNANIGNSALASSVMD
CCCCEEECHHHHHHHHHCCCEEEEEECCCCCCCCEEECCEEEEEEECCCCCHHHHHHHHH
EVEKMIWAIRWGADTVMDLSTGAHIHETREWILRNSPVPIGTVPIYQALEKVAGVAEDLS
HHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC
WDVFRDTLVEQAEQGVDYFTIHAGVRLEHLPLTSRRLTGIVSRGGSIMAKWCHAHRCESF
HHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
LYTRFEEICEIMKAYDVSFSLGDGMRPGSLHDANDEAQFAELKTLGELTRLAWKHDVQTM
HHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IEGPGHVPLHLIRENMELQLEQCHEAPFYTLGPLVTDVAPGYDHITSAIGGAMIGWLGTS
HCCCCCCCHHHHHCCHHHHHHHHCCCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
MLCYVTRKEHLGLPDKDDVKEGIVTFKIAAHAADLAKGHPGARLRDDALSKARFEFRWKD
HEEEEECHHHCCCCCHHHHHHCCEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHEEEEECC
QFNLALDPEMPQRLHDLTLPAEADKASHFCSMCGPDFCAMRITRNIREQAGA
CCCEEECCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA