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The map label for this gene is pnp

Identifier: 118579404

GI number: 118579404

Start: 1010734

End: 1012854

Strand: Direct

Name: pnp

Synonym: Ppro_0968

Alternate gene names: 118579404

Gene position: 1010734-1012854 (Clockwise)

Preceding gene: 118579403

Following gene: 118579405

Centisome position: 25.22

GC content: 61.95

Gene sequence:

>2121_bases
ATGGAACACGTTGTAGATGTTGAGTTTGGCGGCAAGATGGTAACAATCTCCACCGGCAAGATGGCCAAGCAGGCCAACGG
TGCGGTGGTGGTGAAGAGTGGCGACACAATGGTTCTGGTGACCGCCGTGGCCCAGAAAGAGGCCAAAGAGGGGCAGGATT
TCTTTCCCCTGACCGTCAACTATACCGAGAAGGCCTATGCCGGCGGGAAGATCCCGGGCAGCTTCTTCAGGCGCGAGGCG
CGCCCCTCCGACCCGGAGACGCTGACCTGCCGCTTGATCGACCGTCCCATCCGCCCGCTGTTTCCCGAAAATTTCTTGAA
CGACACCCAGATCATTGCAACGGTGGTCTCCGCTGATAAGGACCACGATCCCCGGATACTCTCCATGCTGGGCGCATCGG
CCGCGCTGGAGGTCTCCGACATTCCCTTCCAGGGGCCCATCGCCGGCGTCAAAGTGGGGCGGGTGGATGGTAGACTGATC
TGCAACCCGACTGCTGACGAACTTGAACTGAGCGATATGGAGATCGTGGTCGCCGCCAGCCGGGATGCCATCATCATGGT
TGAGGGTGAGGCCAGATTCGTCTCCGAGGATGATATGCTGGATGCCATCTTCTTCGGCCATGCGGCGGTACAGCCGGTTC
TGGAGGCCCAGGAAAAGCTGAAGCAGCTTGCCGGCGTTGCCAAGCGTGACGTGCCTCCTCCCGTCGTCGACCAGGCCCTG
CTGGCCAGGGTGCGCGAACTGGCCAGCGAGCGCATGTCAGCCGCGGTCAAGATCAAGAGCAAGCAGGAACGCCACAACCA
GATCGACCTGATCACGGCCGAGACGACCGCGTCCCTGGCCGCTGAGTTCGAGGGGAACGAGAAGCAGATTCGCGCTTTTC
TGGGAGACCTGGAGTACGAATGCGTTCGCGCCGATGTTCTGAACAGCGGGGTGCGCATCGATGGCCGCGACACGGTCACC
ATCCGTCCCATCGCCACCGAGGCGGGCCTGCTCCCCCGCACCCACGGCTCGGCCCTGTTCACCCGCGGCGAGACCCAGGC
CCTGGTGGTCACCACCCTGGGCACCTCCAGCGACGAACAGCGCATGGACTCGCTCTACGGTGAATACCGCAAGCGTTTCC
TGTTACACTACAATTTCCCTCCCTTCTCCGTGGGCGAAACCAGCTTCCGCCTCGGACCGGGGCGTCGCGAGATCGGCCAC
GGCATGCTCGCCGAGCGGGCGCTCTCGGCGATCCTCCCCAAGCATGACGATTTCCCCTACACCATCCGGATCGTATCCGA
GACCCTGGAGAGCAACGGCTCCTCCTCCATGGCGGCAGTCTGCGGCGGCTGCATGTCGCTGATGGATGCCGGTGTGCCCA
TCTCCGCTCCGGTTGCCGGCATCGCCATGGGGTTGATCAAGGAGGGCGAGAAGGTCGCCATCCTCTCCGACATCCTGGGT
GACGAGGACCATCTGGGCGACATGGACTTCAAGGTGGCCGGCAGCAGCGACGGGATCACCGCTCTGCAGATGGATATCAA
GATCGGCGGCGTCACCAGGGAGATCATGCAGAAGGCCCTGGCCCAGGCCCGCGAGGGGCGATTGCACATCCTGGGCAAGA
TGGCCGAGACCCTGGGTGCTCCCCGGCCGGAGATGTCGTCCTTTGCTCCGCGCATCACCACCATTTGGGTCAAGACCGAC
AAGATCAGGGACGTTATCGGTACGGGCGGCAAGAATATCCGCAACATCACCGAGACTACCGGCGTTACGGTGGATATCGA
GGATACGGGTCGCATCAACATCGCCAGTACCAGCAAGGAAGCCTGTGATCTCGCCATCCAGATGATCCGCGGGCTGACCG
ACGAGGCCGAAGAAGGCAAGCTGTACATGGGTATTGTCAAGAAGATCATGGATTTCGGTGCTTTTGTGGAAATCCTGCCC
GGTACCGATGGACTCGTGCACATCTCCGAACTGGACACCAAGCGTGTCAAGACGGTTACCGAGGTGCTCAACGAGGGTGA
CCGGGTACTGGTCAAATGCATCGGTGTGGACAAGAACGGCAAGGTCAAGCTCTCCCGCAAGGAGGCTCTTGGGCTGAACC
CGGACGGCACACCGGCAACGGACGCGCCTGCGGGCGAGTAG

Upstream 100 bases:

>100_bases
CGCCTCCTTCCTGAAACGGCTACTGCTGCTGATTGAGGAGGGAGACGGCGGGGACATTGTAGCGACGGCCCCAACGCAAC
AGTCAACAGGGAGGTACTCC

Downstream 100 bases:

>100_bases
TCCTCAGTTGTATCTGTTTGCTCACACGGGCGAAGCGCTTGCTTCGCCCTTTTTTAAGTCGGGACGGGATGGCTGTCCCG
ATTCTGTTCCTGGTTGCTAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MEHVVDVEFGGKMVTISTGKMAKQANGAVVVKSGDTMVLVTAVAQKEAKEGQDFFPLTVNYTEKAYAGGKIPGSFFRREA
RPSDPETLTCRLIDRPIRPLFPENFLNDTQIIATVVSADKDHDPRILSMLGASAALEVSDIPFQGPIAGVKVGRVDGRLI
CNPTADELELSDMEIVVAASRDAIIMVEGEARFVSEDDMLDAIFFGHAAVQPVLEAQEKLKQLAGVAKRDVPPPVVDQAL
LARVRELASERMSAAVKIKSKQERHNQIDLITAETTASLAAEFEGNEKQIRAFLGDLEYECVRADVLNSGVRIDGRDTVT
IRPIATEAGLLPRTHGSALFTRGETQALVVTTLGTSSDEQRMDSLYGEYRKRFLLHYNFPPFSVGETSFRLGPGRREIGH
GMLAERALSAILPKHDDFPYTIRIVSETLESNGSSSMAAVCGGCMSLMDAGVPISAPVAGIAMGLIKEGEKVAILSDILG
DEDHLGDMDFKVAGSSDGITALQMDIKIGGVTREIMQKALAQAREGRLHILGKMAETLGAPRPEMSSFAPRITTIWVKTD
KIRDVIGTGGKNIRNITETTGVTVDIEDTGRINIASTSKEACDLAIQMIRGLTDEAEEGKLYMGIVKKIMDFGAFVEILP
GTDGLVHISELDTKRVKTVTEVLNEGDRVLVKCIGVDKNGKVKLSRKEALGLNPDGTPATDAPAGE

Sequences:

>Translated_706_residues
MEHVVDVEFGGKMVTISTGKMAKQANGAVVVKSGDTMVLVTAVAQKEAKEGQDFFPLTVNYTEKAYAGGKIPGSFFRREA
RPSDPETLTCRLIDRPIRPLFPENFLNDTQIIATVVSADKDHDPRILSMLGASAALEVSDIPFQGPIAGVKVGRVDGRLI
CNPTADELELSDMEIVVAASRDAIIMVEGEARFVSEDDMLDAIFFGHAAVQPVLEAQEKLKQLAGVAKRDVPPPVVDQAL
LARVRELASERMSAAVKIKSKQERHNQIDLITAETTASLAAEFEGNEKQIRAFLGDLEYECVRADVLNSGVRIDGRDTVT
IRPIATEAGLLPRTHGSALFTRGETQALVVTTLGTSSDEQRMDSLYGEYRKRFLLHYNFPPFSVGETSFRLGPGRREIGH
GMLAERALSAILPKHDDFPYTIRIVSETLESNGSSSMAAVCGGCMSLMDAGVPISAPVAGIAMGLIKEGEKVAILSDILG
DEDHLGDMDFKVAGSSDGITALQMDIKIGGVTREIMQKALAQAREGRLHILGKMAETLGAPRPEMSSFAPRITTIWVKTD
KIRDVIGTGGKNIRNITETTGVTVDIEDTGRINIASTSKEACDLAIQMIRGLTDEAEEGKLYMGIVKKIMDFGAFVEILP
GTDGLVHISELDTKRVKTVTEVLNEGDRVLVKCIGVDKNGKVKLSRKEALGLNPDGTPATDAPAGE
>Mature_706_residues
MEHVVDVEFGGKMVTISTGKMAKQANGAVVVKSGDTMVLVTAVAQKEAKEGQDFFPLTVNYTEKAYAGGKIPGSFFRREA
RPSDPETLTCRLIDRPIRPLFPENFLNDTQIIATVVSADKDHDPRILSMLGASAALEVSDIPFQGPIAGVKVGRVDGRLI
CNPTADELELSDMEIVVAASRDAIIMVEGEARFVSEDDMLDAIFFGHAAVQPVLEAQEKLKQLAGVAKRDVPPPVVDQAL
LARVRELASERMSAAVKIKSKQERHNQIDLITAETTASLAAEFEGNEKQIRAFLGDLEYECVRADVLNSGVRIDGRDTVT
IRPIATEAGLLPRTHGSALFTRGETQALVVTTLGTSSDEQRMDSLYGEYRKRFLLHYNFPPFSVGETSFRLGPGRREIGH
GMLAERALSAILPKHDDFPYTIRIVSETLESNGSSSMAAVCGGCMSLMDAGVPISAPVAGIAMGLIKEGEKVAILSDILG
DEDHLGDMDFKVAGSSDGITALQMDIKIGGVTREIMQKALAQAREGRLHILGKMAETLGAPRPEMSSFAPRITTIWVKTD
KIRDVIGTGGKNIRNITETTGVTVDIEDTGRINIASTSKEACDLAIQMIRGLTDEAEEGKLYMGIVKKIMDFGAFVEILP
GTDGLVHISELDTKRVKTVTEVLNEGDRVLVKCIGVDKNGKVKLSRKEALGLNPDGTPATDAPAGE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=712, Percent_Identity=38.623595505618, Blast_Score=458, Evalue=1e-129,
Organism=Escherichia coli, GI145693187, Length=683, Percent_Identity=56.8081991215227, Blast_Score=761, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=724, Percent_Identity=35.3591160220994, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI281362905, Length=711, Percent_Identity=37.6933895921238, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651641, Length=711, Percent_Identity=37.6933895921238, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651643, Length=711, Percent_Identity=37.6933895921238, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI161079377, Length=659, Percent_Identity=36.4188163884674, Blast_Score=404, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_PELPD (A1AMM6)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_900654.1
- ProteinModelPortal:   A1AMM6
- STRING:   A1AMM6
- GeneID:   4574253
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_0968
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   A1AMM6
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 76129; Mature: 76129

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHVVDVEFGGKMVTISTGKMAKQANGAVVVKSGDTMVLVTAVAQKEAKEGQDFFPLTVN
CCCEEEEECCCEEEEEECCCHHHCCCCEEEEECCCCEEEEEEHHHHHHHCCCCEEEEEEE
YTEKAYAGGKIPGSFFRREARPSDPETLTCRLIDRPIRPLFPENFLNDTQIIATVVSADK
CCHHHCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCHHHHHHHHHCCC
DHDPRILSMLGASAALEVSDIPFQGPIAGVKVGRVDGRLICNPTADELELSDMEIVVAAS
CCCCHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCEEEECCCCCCEECCCEEEEEEEC
RDAIIMVEGEARFVSEDDMLDAIFFGHAAVQPVLEAQEKLKQLAGVAKRDVPPPVVDQAL
CCEEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LARVRELASERMSAAVKIKSKQERHNQIDLITAETTASLAAEFEGNEKQIRAFLGDLEYE
HHHHHHHHHHHHHHEEEECCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCHHH
CVRADVLNSGVRIDGRDTVTIRPIATEAGLLPRTHGSALFTRGETQALVVTTLGTSSDEQ
HHHHHHHCCCEEECCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCHHH
RMDSLYGEYRKRFLLHYNFPPFSVGETSFRLGPGRREIGHGMLAERALSAILPKHDDFPY
HHHHHHHHHHHEEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCE
TIRIVSETLESNGSSSMAAVCGGCMSLMDAGVPISAPVAGIAMGLIKEGEKVAILSDILG
EEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEHHHCC
DEDHLGDMDFKVAGSSDGITALQMDIKIGGVTREIMQKALAQAREGRLHILGKMAETLGA
CCCCCCCCCEEEECCCCCEEEEEEEEEECCHHHHHHHHHHHHHHCCCEEEHHHHHHHHCC
PRPEMSSFAPRITTIWVKTDKIRDVIGTGGKNIRNITETTGVTVDIEDTGRINIASTSKE
CCCCHHHCCCCEEEEEEEHHHHHHHHCCCCCCCCCHHHHCCEEEEECCCCEEEEECCCHH
ACDLAIQMIRGLTDEAEEGKLYMGIVKKIMDFGAFVEILPGTDGLVHISELDTKRVKTVT
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEECCCHHHHHHHH
EVLNEGDRVLVKCIGVDKNGKVKLSRKEALGLNPDGTPATDAPAGE
HHHCCCCEEEEEEEECCCCCCEEEEHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEHVVDVEFGGKMVTISTGKMAKQANGAVVVKSGDTMVLVTAVAQKEAKEGQDFFPLTVN
CCCEEEEECCCEEEEEECCCHHHCCCCEEEEECCCCEEEEEEHHHHHHHCCCCEEEEEEE
YTEKAYAGGKIPGSFFRREARPSDPETLTCRLIDRPIRPLFPENFLNDTQIIATVVSADK
CCHHHCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCHHHHHHHHHCCC
DHDPRILSMLGASAALEVSDIPFQGPIAGVKVGRVDGRLICNPTADELELSDMEIVVAAS
CCCCHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCEEEECCCCCCEECCCEEEEEEEC
RDAIIMVEGEARFVSEDDMLDAIFFGHAAVQPVLEAQEKLKQLAGVAKRDVPPPVVDQAL
CCEEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LARVRELASERMSAAVKIKSKQERHNQIDLITAETTASLAAEFEGNEKQIRAFLGDLEYE
HHHHHHHHHHHHHHEEEECCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCHHH
CVRADVLNSGVRIDGRDTVTIRPIATEAGLLPRTHGSALFTRGETQALVVTTLGTSSDEQ
HHHHHHHCCCEEECCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCHHH
RMDSLYGEYRKRFLLHYNFPPFSVGETSFRLGPGRREIGHGMLAERALSAILPKHDDFPY
HHHHHHHHHHHEEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCE
TIRIVSETLESNGSSSMAAVCGGCMSLMDAGVPISAPVAGIAMGLIKEGEKVAILSDILG
EEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEHHHCC
DEDHLGDMDFKVAGSSDGITALQMDIKIGGVTREIMQKALAQAREGRLHILGKMAETLGA
CCCCCCCCCEEEECCCCCEEEEEEEEEECCHHHHHHHHHHHHHHCCCEEEHHHHHHHHCC
PRPEMSSFAPRITTIWVKTDKIRDVIGTGGKNIRNITETTGVTVDIEDTGRINIASTSKE
CCCCHHHCCCCEEEEEEEHHHHHHHHCCCCCCCCCHHHHCCEEEEECCCCEEEEECCCHH
ACDLAIQMIRGLTDEAEEGKLYMGIVKKIMDFGAFVEILPGTDGLVHISELDTKRVKTVT
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEECCCHHHHHHHH
EVLNEGDRVLVKCIGVDKNGKVKLSRKEALGLNPDGTPATDAPAGE
HHHCCCCEEEEEEEECCCCCCEEEEHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA