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The map label for this gene is polA [C]

Identifier: 118579386

GI number: 118579386

Start: 994386

End: 995834

Strand: Direct

Name: polA [C]

Synonym: Ppro_0949

Alternate gene names: 118579386

Gene position: 994386-995834 (Clockwise)

Preceding gene: 118579385

Following gene: 118579387

Centisome position: 24.81

GC content: 67.29

Gene sequence:

>1449_bases
GTGAACGCAGCGCCTCGGCTCTATCTGCTGGACGGGTCCAGTTACATTTACCGCGCCTACTATGGCGTGCGGGATTGCGC
CACCTCGGTTGGGATGCCGACCAATGCCGTCTTCGGCTTCACCAGGATGCTGCTCAGCCTCCTGCAGGAGAACCGCCCCG
ATTACCTGGCAGTGGTATTCGATCCTCCCCGGGAAGGGACCTTTCGCCGGCAGATCTATCCCCCCTACAAGGCGCATCGG
GACGACATGCCCACGGATCTCGCGGCCCAGCTGCCGTACATCAGGCAGGTCGTGCAGGCCCTCAATATCCCGGCCCTGGA
AGCGCCTGGTTTCGAGGCTGACGATGTCATCGCCACCCTGGCCCGCCGCTCCGCGGACGAGGGTGCCCGGGTCACGGTCG
TCACCGGCGACAAGGACCTGCTGCAGATCGTCGGCGAGCGGATCGGCCTGCTGGACACCATGAAGGGTCTCCGTTCCGGG
CCCAGGGAAGTGCTGGACCGCTTCGGGGTGCCGCCGGAGCTGGTGCCGGACGTGCTGGGGCTGGCCGGGGACAGCAGCGA
CAATATCCCGGGGGTTCCCGGCATCGGCGAGAAGACCGCTGCCGAGTTGGTCCGGCGCTTCGGTTCTCTGGAGGGGGTGC
TGCGCTGGAAAAGCCTGGTGAATGGCAGGCAGCGCCGGGAGAACCTGTCCGCCTATGGCGATCAGGCCCGGCTCTCCAAA
ACCCTGGCCACTGTCCGCTATGACGTGCCCCTGGAACTGTCCCTGGCTGATCTGTGCCGCCAGCCGCCCAACCTTCCACT
GCTGATTCCCTTGCTGCGCGAGCTGGAATTCGGGGCGCTGGAGGTTGCCTTCACCCCGCCGCCAGCCGGCGTGGTGGAGA
TCTACTGCGATGGCTCGGGTCGGGACTCCGGGCCGGGTGGATATGGGGTGATCCTGCGCTATGGGGAGTCCGAGAAGGAA
CTCAGCGGTTTCGAGCCGGTCGCCACGTCCCAGCGCATGGAGTTGACCGCGGCCATCAGGGGGCTGGAGGCGTTGCACAA
GCCCAGGCAGGTGCGCGTCTTCAGCGATTCGCAGTATCTGGTGCGGGGGATGGCTGAATGGCTCGAGCGCTGGATTCGCA
GCGGTCGCCTGGAGACGCCGGATGCGCTCAAGAATCAGGATCTCTGGCAGCGGCTGGCTGGCCTGTCCGCCAAGCACCAG
GTCAGTTGGGAGTGGGTGCGCGGCCACGACGGGCATCCCTTCAACGAGCGTTGCGACCGGCTGGCGAAACGGGCGCTTGA
GGCGGGGATGGGGGCGGCCGGGGAGAACGGCCCGATGGAGCCGCGGGAACGGGAGAGGGACGAGGAACCGAGCCCGTCGC
CGGTCGCTCCGGCCAGGCCGGCTTCGCTCCCGGTCGTGGAACCGGCTGCCTATGCGGCCGATGCCGACGGGCAGCTCCTG
CTCTGTTGA

Upstream 100 bases:

>100_bases
TACTGCGCAAAAAGATTGTGTATGATCCCCGGGATGTGCGATTCTACCCCACCCGGTTGAGCGTGGCGTTCGCCGGGGAA
TATCCGCGTCGGGAGAGAAT

Downstream 100 bases:

>100_bases
GGTGGTGGTTGCGGAGGGACAGCTTGCCTTCCCTCTGGTCTGGTAGGGGCCGTGGTTTGGGAAAACCCTGTGGAAAGGAA
CGATTACATGATGTCACGCA

Product: ribonuclease H

Products: diphosphate; DNAn+1

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MNAAPRLYLLDGSSYIYRAYYGVRDCATSVGMPTNAVFGFTRMLLSLLQENRPDYLAVVFDPPREGTFRRQIYPPYKAHR
DDMPTDLAAQLPYIRQVVQALNIPALEAPGFEADDVIATLARRSADEGARVTVVTGDKDLLQIVGERIGLLDTMKGLRSG
PREVLDRFGVPPELVPDVLGLAGDSSDNIPGVPGIGEKTAAELVRRFGSLEGVLRWKSLVNGRQRRENLSAYGDQARLSK
TLATVRYDVPLELSLADLCRQPPNLPLLIPLLRELEFGALEVAFTPPPAGVVEIYCDGSGRDSGPGGYGVILRYGESEKE
LSGFEPVATSQRMELTAAIRGLEALHKPRQVRVFSDSQYLVRGMAEWLERWIRSGRLETPDALKNQDLWQRLAGLSAKHQ
VSWEWVRGHDGHPFNERCDRLAKRALEAGMGAAGENGPMEPRERERDEEPSPSPVAPARPASLPVVEPAAYAADADGQLL
LC

Sequences:

>Translated_482_residues
MNAAPRLYLLDGSSYIYRAYYGVRDCATSVGMPTNAVFGFTRMLLSLLQENRPDYLAVVFDPPREGTFRRQIYPPYKAHR
DDMPTDLAAQLPYIRQVVQALNIPALEAPGFEADDVIATLARRSADEGARVTVVTGDKDLLQIVGERIGLLDTMKGLRSG
PREVLDRFGVPPELVPDVLGLAGDSSDNIPGVPGIGEKTAAELVRRFGSLEGVLRWKSLVNGRQRRENLSAYGDQARLSK
TLATVRYDVPLELSLADLCRQPPNLPLLIPLLRELEFGALEVAFTPPPAGVVEIYCDGSGRDSGPGGYGVILRYGESEKE
LSGFEPVATSQRMELTAAIRGLEALHKPRQVRVFSDSQYLVRGMAEWLERWIRSGRLETPDALKNQDLWQRLAGLSAKHQ
VSWEWVRGHDGHPFNERCDRLAKRALEAGMGAAGENGPMEPRERERDEEPSPSPVAPARPASLPVVEPAAYAADADGQLL
LC
>Mature_482_residues
MNAAPRLYLLDGSSYIYRAYYGVRDCATSVGMPTNAVFGFTRMLLSLLQENRPDYLAVVFDPPREGTFRRQIYPPYKAHR
DDMPTDLAAQLPYIRQVVQALNIPALEAPGFEADDVIATLARRSADEGARVTVVTGDKDLLQIVGERIGLLDTMKGLRSG
PREVLDRFGVPPELVPDVLGLAGDSSDNIPGVPGIGEKTAAELVRRFGSLEGVLRWKSLVNGRQRRENLSAYGDQARLSK
TLATVRYDVPLELSLADLCRQPPNLPLLIPLLRELEFGALEVAFTPPPAGVVEIYCDGSGRDSGPGGYGVILRYGESEKE
LSGFEPVATSQRMELTAAIRGLEALHKPRQVRVFSDSQYLVRGMAEWLERWIRSGRLETPDALKNQDLWQRLAGLSAKHQ
VSWEWVRGHDGHPFNERCDRLAKRALEAGMGAAGENGPMEPRERERDEEPSPSPVAPARPASLPVVEPAAYAADADGQLL
LC

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=277, Percent_Identity=44.4043321299639, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1786408, Length=136, Percent_Identity=49.2647058823529, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI226510970, Length=216, Percent_Identity=32.8703703703704, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918
- InterPro:   IPR003583 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N [H]

EC number: 2.7.7.7

Molecular weight: Translated: 53047; Mature: 53047

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAPRLYLLDGSSYIYRAYYGVRDCATSVGMPTNAVFGFTRMLLSLLQENRPDYLAVVF
CCCCCEEEEEECCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
DPPREGTFRRQIYPPYKAHRDDMPTDLAAQLPYIRQVVQALNIPALEAPGFEADDVIATL
CCCCCCCCCCCCCCCHHHCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
ARRSADEGARVTVVTGDKDLLQIVGERIGLLDTMKGLRSGPREVLDRFGVPPELVPDVLG
HHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHH
LAGDSSDNIPGVPGIGEKTAAELVRRFGSLEGVLRWKSLVNGRQRRENLSAYGDQARLSK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
TLATVRYDVPLELSLADLCRQPPNLPLLIPLLRELEFGALEVAFTPPPAGVVEIYCDGSG
HHHHHEECCCCEECHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCC
RDSGPGGYGVILRYGESEKELSGFEPVATSQRMELTAAIRGLEALHKPRQVRVFSDSQYL
CCCCCCCEEEEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
VRGMAEWLERWIRSGRLETPDALKNQDLWQRLAGLSAKHQVSWEWVRGHDGHPFNERCDR
HHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHH
LAKRALEAGMGAAGENGPMEPRERERDEEPSPSPVAPARPASLPVVEPAAYAADADGQLL
HHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEE
LC
EC
>Mature Secondary Structure
MNAAPRLYLLDGSSYIYRAYYGVRDCATSVGMPTNAVFGFTRMLLSLLQENRPDYLAVVF
CCCCCEEEEEECCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
DPPREGTFRRQIYPPYKAHRDDMPTDLAAQLPYIRQVVQALNIPALEAPGFEADDVIATL
CCCCCCCCCCCCCCCHHHCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
ARRSADEGARVTVVTGDKDLLQIVGERIGLLDTMKGLRSGPREVLDRFGVPPELVPDVLG
HHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHH
LAGDSSDNIPGVPGIGEKTAAELVRRFGSLEGVLRWKSLVNGRQRRENLSAYGDQARLSK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
TLATVRYDVPLELSLADLCRQPPNLPLLIPLLRELEFGALEVAFTPPPAGVVEIYCDGSG
HHHHHEECCCCEECHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCC
RDSGPGGYGVILRYGESEKELSGFEPVATSQRMELTAAIRGLEALHKPRQVRVFSDSQYL
CCCCCCCEEEEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
VRGMAEWLERWIRSGRLETPDALKNQDLWQRLAGLSAKHQVSWEWVRGHDGHPFNERCDR
HHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHH
LAKRALEAGMGAAGENGPMEPRERERDEEPSPSPVAPARPASLPVVEPAAYAADADGQLL
HHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEE
LC
EC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]